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The map label for this gene is gutB [H]

Identifier: 116624463

GI number: 116624463

Start: 6746239

End: 6747270

Strand: Direct

Name: gutB [H]

Synonym: Acid_5385

Alternate gene names: 116624463

Gene position: 6746239-6747270 (Clockwise)

Preceding gene: 116624457

Following gene: 116624464

Centisome position: 67.69

GC content: 65.21

Gene sequence:

>1032_bases
ATGCTCGTAGCCGAACTTATCGCGCAACGTCAGTTCCGATTCACCGAAATTCCGATCGAAGACCCCGGCCCCGGCGAAGT
CCAGGTGCGGGTGAATGCCGTAGGCATTTGCGGAAGCGATCTCCATTCCTACGCCGAAGGTGCGATCGGCGACACTCCGT
GCGAGTACCCCATGGTGCTCGGCCACGAGCCTGCCGGCACCGTCGTGAAGACCGGCACCGGCGTCGGCGGCTGGTCCCGC
GGCGATCGCGCGGCCCTTGAACCGGCTCTCTATTGCTACCACTGCGAGTTCTGCCGCTCCGGCCGCCACAATATCTGCGC
CAACATCCGCTTCCTGAGCAATCCTGGAACTCCCGGTTTCTTCCGCGAGTTCGTCAATCTTCCCGTCTCCAATCTGCTGG
CCATTCCACCCGAGCTTTCACTCGAGCTGGCCACCATCGTCGAACCTCTCGCGGTCGCGCTGCATTCCCTGAAATTCGCC
GCCATCCAGCCCCGCGAAACCGTTGCCGTCTTCGGCGCCGGTCCCATCGGACTCCTCACCATCGCCTGCCTGAAGGACGC
CGGCGCGGGACGCATCTGGGTCGTCGAACCCGTCTCTCACCGCCGCGAACTCGCCCTCCGGATGGGCGCCGCCGCCGCGC
TCGACCCCACTCACCTCGATGCAGCCCGCCAGATCCTCTCCGATACCGCGCGTCGCGGCGTCGATTGCGCCATCGATTGC
GCCGCCCGTGAAGACACCACCAATGCCGCCATCCGCGCCGCTCGCAACGGCGGCCGCGTCGTCCTCACCGGCATCCACTC
CGCCGCCTTCGTCCCCTTCGAAGTCTCCCCCATGCGTCGCAAGGAATTGGCCATCTTCAACGTGCGCCGCTCCTGCCACG
AATCGCACGAGGCCCTCTCTATGCTCACCAGCCAGATGTCGTCATTCGCCCCCCTGGTGACCCACACTCGTCCCCTGCAC
GCTCTGCCCGACGCCTTTACCCTCACCGAACACTACGCCGACGGCGTCGCCAAAATGGTTGTAGTCCCATAG

Upstream 100 bases:

>100_bases
AGCCACTTGAAATACACCTGGAACTTGCGGCCGAAAGGATCCGGGCCCGCTTCAAACTCACGAAAATTCAGCATTCCTCG
CTATTTTACAATGGCTGTAG

Downstream 100 bases:

>100_bases
TATTCCACTGCTCTAGTTTCCTCGCAGCCCGCAAAGACTTCGTCCAAACGAGCCGCGACCGCGAGGGAGCGCAACGTGCA
CTTGATCTTCGGCATCGCCC

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MLVAELIAQRQFRFTEIPIEDPGPGEVQVRVNAVGICGSDLHSYAEGAIGDTPCEYPMVLGHEPAGTVVKTGTGVGGWSR
GDRAALEPALYCYHCEFCRSGRHNICANIRFLSNPGTPGFFREFVNLPVSNLLAIPPELSLELATIVEPLAVALHSLKFA
AIQPRETVAVFGAGPIGLLTIACLKDAGAGRIWVVEPVSHRRELALRMGAAAALDPTHLDAARQILSDTARRGVDCAIDC
AAREDTTNAAIRAARNGGRVVLTGIHSAAFVPFEVSPMRRKELAIFNVRRSCHESHEALSMLTSQMSSFAPLVTHTRPLH
ALPDAFTLTEHYADGVAKMVVVP

Sequences:

>Translated_343_residues
MLVAELIAQRQFRFTEIPIEDPGPGEVQVRVNAVGICGSDLHSYAEGAIGDTPCEYPMVLGHEPAGTVVKTGTGVGGWSR
GDRAALEPALYCYHCEFCRSGRHNICANIRFLSNPGTPGFFREFVNLPVSNLLAIPPELSLELATIVEPLAVALHSLKFA
AIQPRETVAVFGAGPIGLLTIACLKDAGAGRIWVVEPVSHRRELALRMGAAAALDPTHLDAARQILSDTARRGVDCAIDC
AAREDTTNAAIRAARNGGRVVLTGIHSAAFVPFEVSPMRRKELAIFNVRRSCHESHEALSMLTSQMSSFAPLVTHTRPLH
ALPDAFTLTEHYADGVAKMVVVP
>Mature_343_residues
MLVAELIAQRQFRFTEIPIEDPGPGEVQVRVNAVGICGSDLHSYAEGAIGDTPCEYPMVLGHEPAGTVVKTGTGVGGWSR
GDRAALEPALYCYHCEFCRSGRHNICANIRFLSNPGTPGFFREFVNLPVSNLLAIPPELSLELATIVEPLAVALHSLKFA
AIQPRETVAVFGAGPIGLLTIACLKDAGAGRIWVVEPVSHRRELALRMGAAAALDPTHLDAARQILSDTARRGVDCAIDC
AAREDTTNAAIRAARNGGRVVLTGIHSAAFVPFEVSPMRRKELAIFNVRRSCHESHEALSMLTSQMSSFAPLVTHTRPLH
ALPDAFTLTEHYADGVAKMVVVP

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=336, Percent_Identity=33.9285714285714, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI71565152, Length=354, Percent_Identity=25.9887005649718, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI4501929, Length=349, Percent_Identity=26.0744985673352, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI156523966, Length=347, Percent_Identity=26.2247838616715, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI4501939, Length=347, Percent_Identity=26.2247838616715, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI4501933, Length=348, Percent_Identity=25.2873563218391, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI34577061, Length=349, Percent_Identity=24.9283667621776, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI71743840, Length=355, Percent_Identity=24.7887323943662, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI262073058, Length=355, Percent_Identity=24.7887323943662, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788073, Length=332, Percent_Identity=28.9156626506024, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790045, Length=331, Percent_Identity=28.0966767371601, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1787863, Length=326, Percent_Identity=30.6748466257669, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI226510992, Length=329, Percent_Identity=29.483282674772, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790718, Length=324, Percent_Identity=27.4691358024691, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786825, Length=376, Percent_Identity=29.5212765957447, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788407, Length=314, Percent_Identity=27.7070063694268, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786552, Length=350, Percent_Identity=29.7142857142857, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI87082125, Length=358, Percent_Identity=26.536312849162, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788075, Length=335, Percent_Identity=24.7761194029851, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081918, Length=244, Percent_Identity=28.6885245901639, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786518, Length=112, Percent_Identity=33.0357142857143, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=332, Percent_Identity=32.8313253012048, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17562878, Length=334, Percent_Identity=33.5329341317365, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25146526, Length=347, Percent_Identity=24.207492795389, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17562584, Length=296, Percent_Identity=26.6891891891892, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71988145, Length=285, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562582, Length=277, Percent_Identity=26.7148014440433, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71997431, Length=349, Percent_Identity=24.0687679083095, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17565904, Length=307, Percent_Identity=24.4299674267101, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323099, Length=323, Percent_Identity=30.3405572755418, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322619, Length=325, Percent_Identity=29.2307692307692, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319955, Length=325, Percent_Identity=28.9230769230769, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319257, Length=308, Percent_Identity=32.7922077922078, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319258, Length=284, Percent_Identity=31.3380281690141, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319621, Length=331, Percent_Identity=27.7945619335347, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323729, Length=270, Percent_Identity=28.8888888888889, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320033, Length=351, Percent_Identity=24.5014245014245, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323961, Length=280, Percent_Identity=28.2142857142857, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=281, Percent_Identity=28.1138790035587, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17737897, Length=343, Percent_Identity=32.0699708454811, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17137530, Length=344, Percent_Identity=31.9767441860465, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17737895, Length=357, Percent_Identity=26.890756302521, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI45550770, Length=336, Percent_Identity=25.297619047619, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI45551930, Length=336, Percent_Identity=25.297619047619, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI221457811, Length=336, Percent_Identity=25.297619047619, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 36828; Mature: 36828

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVAELIAQRQFRFTEIPIEDPGPGEVQVRVNAVGICGSDLHSYAEGAIGDTPCEYPMVL
CCHHHHHHHCCCEEEECCCCCCCCCEEEEEEEEEEECCHHHHHHHCCCCCCCCCCCCEEE
GHEPAGTVVKTGTGVGGWSRGDRAALEPALYCYHCEFCRSGRHNICANIRFLSNPGTPGF
CCCCCCCEEECCCCCCCCCCCCCHHHCHHHHEEEEHHHHCCCCCEEEEEEEECCCCCCHH
FREFVNLPVSNLLAIPPELSLELATIVEPLAVALHSLKFAAIQPRETVAVFGAGPIGLLT
HHHHHHCCHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCHHHHHH
IACLKDAGAGRIWVVEPVSHRRELALRMGAAAALDPTHLDAARQILSDTARRGVDCAIDC
HHHHHCCCCCEEEEECCHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHCCCCEEEEE
AAREDTTNAAIRAARNGGRVVLTGIHSAAFVPFEVSPMRRKELAIFNVRRSCHESHEALS
CCCCCCCHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
MLTSQMSSFAPLVTHTRPLHALPDAFTLTEHYADGVAKMVVVP
HHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEEC
>Mature Secondary Structure
MLVAELIAQRQFRFTEIPIEDPGPGEVQVRVNAVGICGSDLHSYAEGAIGDTPCEYPMVL
CCHHHHHHHCCCEEEECCCCCCCCCEEEEEEEEEEECCHHHHHHHCCCCCCCCCCCCEEE
GHEPAGTVVKTGTGVGGWSRGDRAALEPALYCYHCEFCRSGRHNICANIRFLSNPGTPGF
CCCCCCCEEECCCCCCCCCCCCCHHHCHHHHEEEEHHHHCCCCCEEEEEEEECCCCCCHH
FREFVNLPVSNLLAIPPELSLELATIVEPLAVALHSLKFAAIQPRETVAVFGAGPIGLLT
HHHHHHCCHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCHHHHHH
IACLKDAGAGRIWVVEPVSHRRELALRMGAAAALDPTHLDAARQILSDTARRGVDCAIDC
HHHHHCCCCCEEEEECCHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHCCCCEEEEE
AAREDTTNAAIRAARNGGRVVLTGIHSAAFVPFEVSPMRRKELAIFNVRRSCHESHEALS
CCCCCCCHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
MLTSQMSSFAPLVTHTRPLHALPDAFTLTEHYADGVAKMVVVP
HHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]