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The map label for this gene is nuoB2

Identifier: 116624397

GI number: 116624397

Start: 6666593

End: 6667147

Strand: Direct

Name: nuoB2

Synonym: Acid_5318

Alternate gene names: 116624397

Gene position: 6666593-6667147 (Clockwise)

Preceding gene: 116624394

Following gene: 116624409

Centisome position: 66.9

GC content: 59.64

Gene sequence:

>555_bases
ATGGAAAAACCCATGGCCAGCAGCGGTCCCGCGGAGCTGTTTACGCGCGTAGACGACTATTCGGTCGCCAATGCCGCGGC
GGCAGCAAACGAAGGGATCGTGATGACGACGCTGGATGCCGCCGTCAATTGGGCCCGCAAGAACTCAATCTGGCCGATGA
CCTTTGGCCTTGCCTGCTGCGCGATCGAGATGATGTCGATGAGCGCTTCACGGTTCGATATCGCGCGCTTCGGCGCCGAG
GTCTTCCGCGGCTCGCCGCGGCAGAGCGACCTCATGATCATCGCCGGCCGCGTCAGCAACAAGATGGCCCCGGTCATCCG
CCACCTCTATCAACAGATGCCCGAGCCCAAATGGGCTATCTCAATGGGGGCCTGTGCTACCTCGACGGGCGTGTTCAACA
ACTATGCGCTGATCCCGGTAAACCAGGTGATCCCTATCGACGTCTTCGTCCCCGGCTGCCCGCCGCGCCCCGAACAGTTG
ATCTACGCACTCATGATGCTGCAGGAGAAGATCAAGAATCAGAGCGGCACCGTAAAGTCTGTATTGAATTTGTAA

Upstream 100 bases:

>100_bases
TTCCGTACCAGGAAGCGGCTCGGTCCAGGAGTTGCTCGAATGTGTCGGTGGGCAATCTTTGATTTTACAGGACTACTGCC
GCTCATGCGAAAATGAAGAT

Downstream 100 bases:

>100_bases
CGCCGGACGCTCCCTTACTTTACGGTCGCGGCTTCCATGAGAAGGCCCCCCTCTGTCAAGAAAATAATCTACAAGGGCTG
AGGGGGTTTCAGGGGGCTTG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MEKPMASSGPAELFTRVDDYSVANAAAAANEGIVMTTLDAAVNWARKNSIWPMTFGLACCAIEMMSMSASRFDIARFGAE
VFRGSPRQSDLMIIAGRVSNKMAPVIRHLYQQMPEPKWAISMGACATSTGVFNNYALIPVNQVIPIDVFVPGCPPRPEQL
IYALMMLQEKIKNQSGTVKSVLNL

Sequences:

>Translated_184_residues
MEKPMASSGPAELFTRVDDYSVANAAAAANEGIVMTTLDAAVNWARKNSIWPMTFGLACCAIEMMSMSASRFDIARFGAE
VFRGSPRQSDLMIIAGRVSNKMAPVIRHLYQQMPEPKWAISMGACATSTGVFNNYALIPVNQVIPIDVFVPGCPPRPEQL
IYALMMLQEKIKNQSGTVKSVLNL
>Mature_184_residues
MEKPMASSGPAELFTRVDDYSVANAAAAANEGIVMTTLDAAVNWARKNSIWPMTFGLACCAIEMMSMSASRFDIARFGAE
VFRGSPRQSDLMIIAGRVSNKMAPVIRHLYQQMPEPKWAISMGACATSTGVFNNYALIPVNQVIPIDVFVPGCPPRPEQL
IYALMMLQEKIKNQSGTVKSVLNL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=53.8461538461538, Blast_Score=182, Evalue=1e-46,
Organism=Escherichia coli, GI1788624, Length=146, Percent_Identity=56.1643835616438, Blast_Score=185, Evalue=2e-48,
Organism=Escherichia coli, GI1789074, Length=124, Percent_Identity=37.0967741935484, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788834, Length=124, Percent_Identity=36.2903225806452, Blast_Score=80, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17509685, Length=143, Percent_Identity=53.8461538461538, Blast_Score=172, Evalue=9e-44,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=52.4822695035461, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=52.4822695035461, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24651058, Length=138, Percent_Identity=52.1739130434783, Blast_Score=173, Evalue=6e-44,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB2_SOLUE (Q01VP7)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_826553.1
- ProteinModelPortal:   Q01VP7
- SMR:   Q01VP7
- STRING:   Q01VP7
- GeneID:   4426472
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_5318
- NMPDR:   fig|234267.9.peg.5047
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   PEAMLDA
- PhylomeDB:   Q01VP7
- ProtClustDB:   PRK06411
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20078; Mature: 20078

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
7.1 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
7.1 %Met     (Mature Protein)
9.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPMASSGPAELFTRVDDYSVANAAAAANEGIVMTTLDAAVNWARKNSIWPMTFGLACC
CCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHH
AIEMMSMSASRFDIARFGAEVFRGSPRQSDLMIIAGRVSNKMAPVIRHLYQQMPEPKWAI
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
SMGACATSTGVFNNYALIPVNQVIPIDVFVPGCPPRPEQLIYALMMLQEKIKNQSGTVKS
EECCHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
VLNL
HHCC
>Mature Secondary Structure
MEKPMASSGPAELFTRVDDYSVANAAAAANEGIVMTTLDAAVNWARKNSIWPMTFGLACC
CCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHH
AIEMMSMSASRFDIARFGAEVFRGSPRQSDLMIIAGRVSNKMAPVIRHLYQQMPEPKWAI
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
SMGACATSTGVFNNYALIPVNQVIPIDVFVPGCPPRPEQLIYALMMLQEKIKNQSGTVKS
EECCHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
VLNL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA