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The map label for this gene is metK [H]

Identifier: 116624354

GI number: 116624354

Start: 6612082

End: 6613251

Strand: Direct

Name: metK [H]

Synonym: Acid_5274

Alternate gene names: 116624354

Gene position: 6612082-6613251 (Clockwise)

Preceding gene: 116624353

Following gene: 116624356

Centisome position: 66.35

GC content: 63.25

Gene sequence:

>1170_bases
TTGGCAGACACTCAAAGACATATCTTCACCTCGGAATCGGTGACCGAAGGCCACCCCGACAAAATTGCCGACCAGATCTC
GGACGCGGTCCTGGATGCCGTACTGGCGGACGACCCGACCGGACGCGTGGCGTGCGAAGTCCTGGTCACGACCGGCACTT
GCATCGTCGCCGGCGAGATCACCACCAAGACGTACGTGGACGTGCAGCGCATCGCGCGCTCCACCATCGAATACGTGGGC
TACAACGACGCCACTTACGGTTTCGACTCCAACACCTGCGGCGTTCACAACCTGATCCAGTCCCAGTCGCCCGATATTTC
GATGGGCGTGGATACCGGCGGCGCCGGCGACCAGGGTCTCATGTTCGGCTACGCGTGCGACGAAACTCCCGAGCTCATGC
CGCTGCCGATCATGATGGCGCACAAGCTCTGCCAGCGCCTCAGCGATGCCCGCCGCGATGGAACCCTGAACTTCCTGCGT
CCCGACGGCAAGAGCCAGGTCTCCGTGGAATACGTGGACGGCAAGCCGAAGCGCATCGAAGCCGTCGTCATCTCCACCCA
GCACAGCCCCGATGTCTCCACCGAAGACCTGCGCCGTGAAGTGCGCAAGCACATCATCGACGCCGTGGTCCCGGCCAACA
TGGTGGATGCCGACACCAAGTACCACATCAACCCGACCGGCCGCTTCGTGGTCGGCGGACCGCACGGCGATACCGGGCTT
ACCGGCCGCAAGATCATCGTCGATACCTATGGCGGCATGGGCCGTCACGGCGGCGGCGCTTTCAGCGGCAAGGATCCGAC
GAAGGTCGATCGCTCGGCCTGCTACATGGCGCGCTATATCGCCAAGAATATCGTGGCCTCCAAGCTGGCTTCGCGCTGCG
AAGTGCAGCTCGCCTACGCGATCGGCGTGGCCGAGCCGGTATCGGTCATGGTCAACACGTTCGGCACCGGCACCATCCCC
GATGCCCGCTTCACCGAGCTGGTCCGCAAGAATTTCAAGCTGACCCCGGCCGGAATCATCGAATCGCTCAACCTGCGTCG
TCCGATCTACCGCAAGACGGCCGCCTTCGGCCACTTCGGGCGCACGGACGAAACCTTTACCTGGGAAAAGACGGACAAGG
CCGAGGCACTGAAAGATCAGGCCGGAGTCGCTGCCGCCGCAGCCAAGTAA

Upstream 100 bases:

>100_bases
GCTGACGCCCGCGCAGGCCGAATACCTCGGTGTGCCGGTCGAGGGGCCGTACAAGCCCGATCATTATCGGTACTAATAGT
CAGGAACTGGAGAAATAACC

Downstream 100 bases:

>100_bases
GTAACTTCACTGCTGTAAAAAGGCCTCGCGAGTTTCGGCTCGCGAGGCCTTTTTGTTTTTACGCTCCACCTCGCACCTTT
TCGGCCGATTCCGCGCCAAA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MADTQRHIFTSESVTEGHPDKIADQISDAVLDAVLADDPTGRVACEVLVTTGTCIVAGEITTKTYVDVQRIARSTIEYVG
YNDATYGFDSNTCGVHNLIQSQSPDISMGVDTGGAGDQGLMFGYACDETPELMPLPIMMAHKLCQRLSDARRDGTLNFLR
PDGKSQVSVEYVDGKPKRIEAVVISTQHSPDVSTEDLRREVRKHIIDAVVPANMVDADTKYHINPTGRFVVGGPHGDTGL
TGRKIIVDTYGGMGRHGGGAFSGKDPTKVDRSACYMARYIAKNIVASKLASRCEVQLAYAIGVAEPVSVMVNTFGTGTIP
DARFTELVRKNFKLTPAGIIESLNLRRPIYRKTAAFGHFGRTDETFTWEKTDKAEALKDQAGVAAAAAK

Sequences:

>Translated_389_residues
MADTQRHIFTSESVTEGHPDKIADQISDAVLDAVLADDPTGRVACEVLVTTGTCIVAGEITTKTYVDVQRIARSTIEYVG
YNDATYGFDSNTCGVHNLIQSQSPDISMGVDTGGAGDQGLMFGYACDETPELMPLPIMMAHKLCQRLSDARRDGTLNFLR
PDGKSQVSVEYVDGKPKRIEAVVISTQHSPDVSTEDLRREVRKHIIDAVVPANMVDADTKYHINPTGRFVVGGPHGDTGL
TGRKIIVDTYGGMGRHGGGAFSGKDPTKVDRSACYMARYIAKNIVASKLASRCEVQLAYAIGVAEPVSVMVNTFGTGTIP
DARFTELVRKNFKLTPAGIIESLNLRRPIYRKTAAFGHFGRTDETFTWEKTDKAEALKDQAGVAAAAAK
>Mature_388_residues
ADTQRHIFTSESVTEGHPDKIADQISDAVLDAVLADDPTGRVACEVLVTTGTCIVAGEITTKTYVDVQRIARSTIEYVGY
NDATYGFDSNTCGVHNLIQSQSPDISMGVDTGGAGDQGLMFGYACDETPELMPLPIMMAHKLCQRLSDARRDGTLNFLRP
DGKSQVSVEYVDGKPKRIEAVVISTQHSPDVSTEDLRREVRKHIIDAVVPANMVDADTKYHINPTGRFVVGGPHGDTGLT
GRKIIVDTYGGMGRHGGGAFSGKDPTKVDRSACYMARYIAKNIVASKLASRCEVQLAYAIGVAEPVSVMVNTFGTGTIPD
ARFTELVRKNFKLTPAGIIESLNLRRPIYRKTAAFGHFGRTDETFTWEKTDKAEALKDQAGVAAAAAK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=55.3763440860215, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI5174529, Length=372, Percent_Identity=54.0322580645161, Blast_Score=401, Evalue=1e-112,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=61.0966057441253, Blast_Score=488, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI25145633, Length=385, Percent_Identity=55.3246753246753, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=55.3191489361702, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=55.3191489361702, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17551082, Length=377, Percent_Identity=53.8461538461538, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=51.063829787234, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=335, Percent_Identity=54.0298507462687, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=53.9877300613497, Blast_Score=348, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=55.1155115511551, Blast_Score=323, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=92, Percent_Identity=51.0869565217391, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320710, Length=385, Percent_Identity=55.0649350649351, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6323209, Length=373, Percent_Identity=55.7640750670241, Blast_Score=419, Evalue=1e-118,
Organism=Drosophila melanogaster, GI45552159, Length=385, Percent_Identity=51.4285714285714, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580529, Length=385, Percent_Identity=51.4285714285714, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580537, Length=385, Percent_Identity=51.4285714285714, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580533, Length=385, Percent_Identity=51.4285714285714, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580535, Length=385, Percent_Identity=51.4285714285714, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580531, Length=385, Percent_Identity=51.4285714285714, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580527, Length=385, Percent_Identity=51.1688311688312, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580525, Length=385, Percent_Identity=51.1688311688312, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580541, Length=366, Percent_Identity=50.5464480874317, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580539, Length=124, Percent_Identity=49.1935483870968, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42023; Mature: 41892

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTQRHIFTSESVTEGHPDKIADQISDAVLDAVLADDPTGRVACEVLVTTGTCIVAGEI
CCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCC
TTKTYVDVQRIARSTIEYVGYNDATYGFDSNTCGVHNLIQSQSPDISMGVDTGGAGDQGL
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCE
MFGYACDETPELMPLPIMMAHKLCQRLSDARRDGTLNFLRPDGKSQVSVEYVDGKPKRIE
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCEEE
AVVISTQHSPDVSTEDLRREVRKHIIDAVVPANMVDADTKYHINPTGRFVVGGPHGDTGL
EEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCCCCC
TGRKIIVDTYGGMGRHGGGAFSGKDPTKVDRSACYMARYIAKNIVASKLASRCEVQLAYA
CCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
IGVAEPVSVMVNTFGTGTIPDARFTELVRKNFKLTPAGIIESLNLRRPIYRKTAAFGHFG
ECCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHCCCC
RTDETFTWEKTDKAEALKDQAGVAAAAAK
CCCCCEEECCCCHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
ADTQRHIFTSESVTEGHPDKIADQISDAVLDAVLADDPTGRVACEVLVTTGTCIVAGEI
CCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCC
TTKTYVDVQRIARSTIEYVGYNDATYGFDSNTCGVHNLIQSQSPDISMGVDTGGAGDQGL
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCE
MFGYACDETPELMPLPIMMAHKLCQRLSDARRDGTLNFLRPDGKSQVSVEYVDGKPKRIE
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCEEE
AVVISTQHSPDVSTEDLRREVRKHIIDAVVPANMVDADTKYHINPTGRFVVGGPHGDTGL
EEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCCCCC
TGRKIIVDTYGGMGRHGGGAFSGKDPTKVDRSACYMARYIAKNIVASKLASRCEVQLAYA
CCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
IGVAEPVSVMVNTFGTGTIPDARFTELVRKNFKLTPAGIIESLNLRRPIYRKTAAFGHFG
ECCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHCCCC
RTDETFTWEKTDKAEALKDQAGVAAAAAK
CCCCCEEECCCCHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA