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The map label for this gene is pilD [H]

Identifier: 116624254

GI number: 116624254

Start: 6495918

End: 6496727

Strand: Direct

Name: pilD [H]

Synonym: Acid_5172

Alternate gene names: 116624254

Gene position: 6495918-6496727 (Clockwise)

Preceding gene: 116624253

Following gene: 116624258

Centisome position: 65.18

GC content: 62.1

Gene sequence:

>810_bases
ATGATTGAAGCCATCCTCGCCCTCATCTTCGGCCTCTTGATCGGCAGCTTCCTCAATGTCTGCATTCACCGCTGGCCGCG
CAATCGCAGCGTGGTGAAGCCGCGCTCGCATTGCGTGCGCTGCCGCAAGACCATCGCCTGGTACGACAACATTCCGCTGC
TCAGCTACCTCGTACTCGGCGGCAAGTGCCGGCACTGCGGCGCCAGGATCTCGTGGCGCTACCCGCTGGTCGAATTCCTC
ACCGGCCTGTTCTTCTTCTATTTCGTCCGAGTCAATGGATTAACCCCGCTCGCCGCCAAGATGTGCGTCTTCAGCGCCAT
CGTGATCGCGCTCATCTTCAGCGACCTCGAAAAACGCATCCTGCCCGATGAATTCACGAAAGGGGGCATCGTGCTCGGGC
TAATCTTTTCGCTCTTCGTCCCCGTGCCCGACATCACCGCGCAGGCCATGTTCTGGATGGCCGGCGGCGAACTGACCGGC
TGGAAACAATCCTTGGCCGAATCCGCCGTCGGCGCGTTCCTGCCCGCGTTTTTCCTGTGGTTCGGCGGCTGGCTCTACTT
CAAGGTGCGCCATCGCGAAGGGCTCGGCTTCGGCGACGTAAAGCTGATCGCGATGGTCGGAAGTTTCCTGGGACTGCGCG
GCGCGCTCCTCACTCTGATCCTCGGCTCCATCGCCGGCTCCGTCATCGGCTACAGCTACATCAAGCTCACCGGCAAGGAC
CCTTCGGAGTACGAACTCCCGTTCGGCACCTTCCTCGGCTTCGCCGCCCTCTTCGCCGGCATCGCGGGCGCCCAACTGCT
GGCTATCTAG

Upstream 100 bases:

>100_bases
GTTGCCGGGTTGCTTGCGGCTTGCTGCAATGCGGGGCAGCCAATCCCTCGCGCTCGACTATTCTTCCACCTCGGCGTTGG
TTCCAGGAGTTCGTCCGTAA

Downstream 100 bases:

>100_bases
GCTCGGTCCCTGATCCTTTGAGTCTTATTCCGCTGCCCCGCGTGTACGGCTACCGCTTTCCAGCACCTCGCGGACTTTTT
GCGTGAAGGCTGCTGGCGTG

Product: type 4 prepilin peptidase 1

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MIEAILALIFGLLIGSFLNVCIHRWPRNRSVVKPRSHCVRCRKTIAWYDNIPLLSYLVLGGKCRHCGARISWRYPLVEFL
TGLFFFYFVRVNGLTPLAAKMCVFSAIVIALIFSDLEKRILPDEFTKGGIVLGLIFSLFVPVPDITAQAMFWMAGGELTG
WKQSLAESAVGAFLPAFFLWFGGWLYFKVRHREGLGFGDVKLIAMVGSFLGLRGALLTLILGSIAGSVIGYSYIKLTGKD
PSEYELPFGTFLGFAALFAGIAGAQLLAI

Sequences:

>Translated_269_residues
MIEAILALIFGLLIGSFLNVCIHRWPRNRSVVKPRSHCVRCRKTIAWYDNIPLLSYLVLGGKCRHCGARISWRYPLVEFL
TGLFFFYFVRVNGLTPLAAKMCVFSAIVIALIFSDLEKRILPDEFTKGGIVLGLIFSLFVPVPDITAQAMFWMAGGELTG
WKQSLAESAVGAFLPAFFLWFGGWLYFKVRHREGLGFGDVKLIAMVGSFLGLRGALLTLILGSIAGSVIGYSYIKLTGKD
PSEYELPFGTFLGFAALFAGIAGAQLLAI
>Mature_269_residues
MIEAILALIFGLLIGSFLNVCIHRWPRNRSVVKPRSHCVRCRKTIAWYDNIPLLSYLVLGGKCRHCGARISWRYPLVEFL
TGLFFFYFVRVNGLTPLAAKMCVFSAIVIALIFSDLEKRILPDEFTKGGIVLGLIFSLFVPVPDITAQAMFWMAGGELTG
WKQSLAESAVGAFLPAFFLWFGGWLYFKVRHREGLGFGDVKLIAMVGSFLGLRGALLTLILGSIAGSVIGYSYIKLTGKD
PSEYELPFGTFLGFAALFAGIAGAQLLAI

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=276, Percent_Identity=35.5072463768116, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 29662; Mature: 29662

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEAILALIFGLLIGSFLNVCIHRWPRNRSVVKPRSHCVRCRKTIAWYDNIPLLSYLVLG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHC
GKCRHCGARISWRYPLVEFLTGLFFFYFVRVNGLTPLAAKMCVFSAIVIALIFSDLEKRI
CCHHHCCCCCEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPDEFTKGGIVLGLIFSLFVPVPDITAQAMFWMAGGELTGWKQSLAESAVGAFLPAFFLW
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
FGGWLYFKVRHREGLGFGDVKLIAMVGSFLGLRGALLTLILGSIAGSVIGYSYIKLTGKD
HCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
PSEYELPFGTFLGFAALFAGIAGAQLLAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIEAILALIFGLLIGSFLNVCIHRWPRNRSVVKPRSHCVRCRKTIAWYDNIPLLSYLVLG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHC
GKCRHCGARISWRYPLVEFLTGLFFFYFVRVNGLTPLAAKMCVFSAIVIALIFSDLEKRI
CCHHHCCCCCEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPDEFTKGGIVLGLIFSLFVPVPDITAQAMFWMAGGELTGWKQSLAESAVGAFLPAFFLW
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
FGGWLYFKVRHREGLGFGDVKLIAMVGSFLGLRGALLTLILGSIAGSVIGYSYIKLTGKD
HCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
PSEYELPFGTFLGFAALFAGIAGAQLLAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA