Click here to switch to the map view.

The map label for this gene is rpoA

Identifier: 116624174

GI number: 116624174

Start: 6426796

End: 6427839

Strand: Reverse

Name: rpoA

Synonym: Acid_5090

Alternate gene names: 116624174

Gene position: 6427839-6426796 (Counterclockwise)

Preceding gene: 116624175

Following gene: 116624173

Centisome position: 64.5

GC content: 59.0

Gene sequence:

>1044_bases
ATGTTTAAGGGCTTTCAAAAACCCAAACGATTGGTCGCCAATACCGAGACCCTCACAGAGCGGTATGGCATGTTTACGGC
CCAGCCTTTTCAACGCGGGTTCGGCACCACGATCGGCAATAGCCTGCGCCGCGTGCTGTTGAGCTCGATCGAAGGCGCGG
CCATCACAGCGGTTCGCATCGAAGGCGTGGAACACGAATTCAGCCCCATCCCGGGCGTAGTGGAAGACGCTACCGATATC
ATTCTCAACCTCAAGCAGATCCCGTTCAAAATTTCGAGCGACGGCATCAAGACCGTGCGCCTCTCCGTGGATTCCCACGG
CGACGTGCGCAGCGGCCAGATCGAAACCGACGCCGATGTCGAAGTGCTGGATCGCGATGTGCACGTCGCCACCGTGAGTG
AAGGCGGCAAGCTGAACATCGAAATGCGCCTCAAGAGCGGCCGCGGCTATGTCAGCGCGGACAAGAATTTCGATGAAGAC
CTGGCGCTCGGCTACATTCCGATCGATTCGGTGCATTCGCCGGTGCGCAAGGTCAACTTCGCCGTGGAGGCAGCGCGCCT
CGGGCAGATGACGGATTACGACAAACTGACGCTCGAAGTCTGGACCAACGGCGCCGTGTCGCCGCAGGATTCCATCGGCT
ACGCCGCGAAGCTGCTGAAAGATCACATGACGATCTTCATCAACTTCGAGGAAGTGCCGGAGCAGACCGAAGAGATCAGC
GAGCGCGGCATGGACAAGATGAACGAAGTCCTGAACCGCTCGGTGGAAGAACTGGAATTGAGCGTTCGTTCTTACAACTG
CCTGAAGAACGCCAACATCCAGAGCATCGGCGAACTGGTGCAGAAGACCGAAGCCGAAATGCTGCGGACCAAGAACTTTG
GCCGCAAGTCGCTCAACGAAATTAAGGAAATTCTGGCAAACATGGGCCTCTCGCTGGGCATGCGGATCGACCAGCATGGT
CGGCTCGTGGCTCCTCCGCCGTCGGCCGGGGGCGGCCCCGACTTCGGACCCGAGGACGACGGCCAGGACCAGATTGGGGA
GTAG

Upstream 100 bases:

>100_bases
TGGTGCAGATCCAATTGAACGAGCAGCTCATCGTCGAGTTGTACTCGAAGTAAGCGGACTTGGACCGAGCCCGCGGAGAC
GCGGGTTCAAGGGAGAATTT

Downstream 100 bases:

>100_bases
CGCATGAGACACAAAGTTGCCGGATATAAATTAAAGCGCGACACGGCCGGGCGAAACTCGCTCCTGCGCAATCTCGCCAC
GAGCGTGATCGAGGCCGAGC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MFKGFQKPKRLVANTETLTERYGMFTAQPFQRGFGTTIGNSLRRVLLSSIEGAAITAVRIEGVEHEFSPIPGVVEDATDI
ILNLKQIPFKISSDGIKTVRLSVDSHGDVRSGQIETDADVEVLDRDVHVATVSEGGKLNIEMRLKSGRGYVSADKNFDED
LALGYIPIDSVHSPVRKVNFAVEAARLGQMTDYDKLTLEVWTNGAVSPQDSIGYAAKLLKDHMTIFINFEEVPEQTEEIS
ERGMDKMNEVLNRSVEELELSVRSYNCLKNANIQSIGELVQKTEAEMLRTKNFGRKSLNEIKEILANMGLSLGMRIDQHG
RLVAPPPSAGGGPDFGPEDDGQDQIGE

Sequences:

>Translated_347_residues
MFKGFQKPKRLVANTETLTERYGMFTAQPFQRGFGTTIGNSLRRVLLSSIEGAAITAVRIEGVEHEFSPIPGVVEDATDI
ILNLKQIPFKISSDGIKTVRLSVDSHGDVRSGQIETDADVEVLDRDVHVATVSEGGKLNIEMRLKSGRGYVSADKNFDED
LALGYIPIDSVHSPVRKVNFAVEAARLGQMTDYDKLTLEVWTNGAVSPQDSIGYAAKLLKDHMTIFINFEEVPEQTEEIS
ERGMDKMNEVLNRSVEELELSVRSYNCLKNANIQSIGELVQKTEAEMLRTKNFGRKSLNEIKEILANMGLSLGMRIDQHG
RLVAPPPSAGGGPDFGPEDDGQDQIGE
>Mature_347_residues
MFKGFQKPKRLVANTETLTERYGMFTAQPFQRGFGTTIGNSLRRVLLSSIEGAAITAVRIEGVEHEFSPIPGVVEDATDI
ILNLKQIPFKISSDGIKTVRLSVDSHGDVRSGQIETDADVEVLDRDVHVATVSEGGKLNIEMRLKSGRGYVSADKNFDED
LALGYIPIDSVHSPVRKVNFAVEAARLGQMTDYDKLTLEVWTNGAVSPQDSIGYAAKLLKDHMTIFINFEEVPEQTEEIS
ERGMDKMNEVLNRSVEELELSVRSYNCLKNANIQSIGELVQKTEAEMLRTKNFGRKSLNEIKEILANMGLSLGMRIDQHG
RLVAPPPSAGGGPDFGPEDDGQDQIGE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=330, Percent_Identity=39.6969696969697, Blast_Score=235, Evalue=3e-63,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_SOLUE (Q01WC0)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_826330.1
- ProteinModelPortal:   Q01WC0
- STRING:   Q01WC0
- GeneID:   4426681
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_5090
- NMPDR:   fig|234267.9.peg.4833
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 38240; Mature: 38240

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGFQKPKRLVANTETLTERYGMFTAQPFQRGFGTTIGNSLRRVLLSSIEGAAITAVRI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEE
EGVEHEFSPIPGVVEDATDIILNLKQIPFKISSDGIKTVRLSVDSHGDVRSGQIETDADV
ECCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCEEEEEEEECCCCCCCCCCCCCCCCH
EVLDRDVHVATVSEGGKLNIEMRLKSGRGYVSADKNFDEDLALGYIPIDSVHSPVRKVNF
HHHCCCEEEEEECCCCEEEEEEEEECCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHHH
AVEAARLGQMTDYDKLTLEVWTNGAVSPQDSIGYAAKLLKDHMTIFINFEEVPEQTEEIS
HHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEHHHCCHHHHHHH
ERGMDKMNEVLNRSVEELELSVRSYNCLKNANIQSIGELVQKTEAEMLRTKNFGRKSLNE
HHCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IKEILANMGLSLGMRIDQHGRLVAPPPSAGGGPDFGPEDDGQDQIGE
HHHHHHHCCCCCCCEECCCCCEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFKGFQKPKRLVANTETLTERYGMFTAQPFQRGFGTTIGNSLRRVLLSSIEGAAITAVRI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEE
EGVEHEFSPIPGVVEDATDIILNLKQIPFKISSDGIKTVRLSVDSHGDVRSGQIETDADV
ECCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCEEEEEEEECCCCCCCCCCCCCCCCH
EVLDRDVHVATVSEGGKLNIEMRLKSGRGYVSADKNFDEDLALGYIPIDSVHSPVRKVNF
HHHCCCEEEEEECCCCEEEEEEEEECCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHHH
AVEAARLGQMTDYDKLTLEVWTNGAVSPQDSIGYAAKLLKDHMTIFINFEEVPEQTEEIS
HHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEHHHCCHHHHHHH
ERGMDKMNEVLNRSVEELELSVRSYNCLKNANIQSIGELVQKTEAEMLRTKNFGRKSLNE
HHCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IKEILANMGLSLGMRIDQHGRLVAPPPSAGGGPDFGPEDDGQDQIGE
HHHHHHHCCCCCCCEECCCCCEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA