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The map label for this gene is fixL [H]

Identifier: 116624169

GI number: 116624169

Start: 6422247

End: 6423674

Strand: Direct

Name: fixL [H]

Synonym: Acid_5085

Alternate gene names: 116624169

Gene position: 6422247-6423674 (Clockwise)

Preceding gene: 116624162

Following gene: 116624170

Centisome position: 64.44

GC content: 64.29

Gene sequence:

>1428_bases
ATGATGGTAACAAGCGCAGCGATGACCCGACTCGACAGGTTCCTGGCATACTCCGAATCGCACCGGCTCACGGTGTGCAT
GACCGCGGCGCTCTGGATCAGCCTCATCGCGTGGTGGGATTCCCTGCTGCCCGATGTCTCGATCGGCTTCCTCTACTTGT
TCCCGGTGCTCTTCTCCGCGGCGGCGCTCAACAACCTGCAGATTCTGGGAGTCGCGCTGCTCTGCGGCTTTCTTCGCAAG
ATCTTCGATCCTCTCGGAGACGCGCCTGGCGCCGCCGAGCGATTCGGAGTAGCAGTGGCCAGCTATGCGATGACCGGCTT
TTTCGTAAACGCGCTCAACCAGAGGCGGCGCCTGCTGGTGCGCCACCTGGACGAATTGGAGCGGCAGATCCGCCTTCGGA
AGGAGGCCGAGCAGCAGGTGCGAATTCTGATCGAGACCAGCCCGCTGGCGATCCTCACGCTGAACCATTCGGGACAGATC
GCCCTGGCCAACGAATCGGCGCGCAACCTGTTTGGCTTCGGCGACGACTCGCTGCAGGGCGCGGACGTCAGCCCGTACCT
GCCGATTTTGAATCGCATGCTGCACAACGCGCGGCCCGGCGCGAACCTAAGGACCAACGTGGAATGCAAGGCGCACCGCC
AGAACGGCGAAGTATTTCTGGCCAACGTGTGGCTCTCCACTTATCGCACCTCCGAAGGTCCGGGACTTGCGGCGGTGGTG
TGGGACGCGAGCGAGAATCTGCGCGACCGCGAAGGCGCGGGTTTCGATTCGATGATGGCCACCTCGCGCGTTCTGATCGG
CGCCATTTCGCACGAGATTCGCAATTTGGCGTCAGCGGCCGCCTCCGCGCACGCGCGCCTTGCCGCCGCCTGCGACGTGA
AGAACGACGAGCAGTACCAGGTGCTGGGCACGCTGATCCACGGGCTGGAGAAGATCGCATCGTCGGGACTGCGCGCGGCG
TCGGATCGCGCGGCGGCGGTAGCGGACCTCAGCACGGTGCTCGATGAAACGCGCATCGTCATCGAGCCGGCCTTGCGCGA
GGCCGGCATCTCGCTGATCTGGGAGGTGGGCGACGCACTGCCGCTGGTGCAGGCCGATCATCACAGCCTGCTCCAGGTCT
TCGTTAATCTCTCGCGGAACAGCGTGCGCGCGCTGGAGGACCGTCCGGTGCGCGAGGTGCGTATCTCGGCGGAAGCGGAG
AGAGACCTCGTGGTGGTGCGCTTTCGGGATTCGGGGCCGGGTGTGGCGAACCCGGACGAGCTCTTTCGTCCGTTCCAGGC
CGGAGCCCATTCAGTGGGGCTCGGGCTCTATATTTCACGCGCAATTCTGCGCGCTCACGGCGGGGGGCTGCGTTACGATT
CCGGGCAGGGTCCAGGCAGCTGCTTTACAGTAGAGCTATGGCCGGTGGAGAATGCATTAGAAGGGTGA

Upstream 100 bases:

>100_bases
GGCAACCGAGATCAAATAGAAACCGAGGACCAGCGTCACCAGAAAGATCAGTATGGAGCGGAACCAACCGCGTTTTCGCT
TCCGGCTTGGGAGCACGACT

Downstream 100 bases:

>100_bases
GTTGCCACTAGTGACAGATCAGGCGCCGATCCGCATCCTGCTGGTGGACGATCATTCCTTGTTTCGGGAGAGCCTGGCGC
AAGCCTTGTCGTCGGAGGAG

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MMVTSAAMTRLDRFLAYSESHRLTVCMTAALWISLIAWWDSLLPDVSIGFLYLFPVLFSAAALNNLQILGVALLCGFLRK
IFDPLGDAPGAAERFGVAVASYAMTGFFVNALNQRRRLLVRHLDELERQIRLRKEAEQQVRILIETSPLAILTLNHSGQI
ALANESARNLFGFGDDSLQGADVSPYLPILNRMLHNARPGANLRTNVECKAHRQNGEVFLANVWLSTYRTSEGPGLAAVV
WDASENLRDREGAGFDSMMATSRVLIGAISHEIRNLASAAASAHARLAAACDVKNDEQYQVLGTLIHGLEKIASSGLRAA
SDRAAAVADLSTVLDETRIVIEPALREAGISLIWEVGDALPLVQADHHSLLQVFVNLSRNSVRALEDRPVREVRISAEAE
RDLVVVRFRDSGPGVANPDELFRPFQAGAHSVGLGLYISRAILRAHGGGLRYDSGQGPGSCFTVELWPVENALEG

Sequences:

>Translated_475_residues
MMVTSAAMTRLDRFLAYSESHRLTVCMTAALWISLIAWWDSLLPDVSIGFLYLFPVLFSAAALNNLQILGVALLCGFLRK
IFDPLGDAPGAAERFGVAVASYAMTGFFVNALNQRRRLLVRHLDELERQIRLRKEAEQQVRILIETSPLAILTLNHSGQI
ALANESARNLFGFGDDSLQGADVSPYLPILNRMLHNARPGANLRTNVECKAHRQNGEVFLANVWLSTYRTSEGPGLAAVV
WDASENLRDREGAGFDSMMATSRVLIGAISHEIRNLASAAASAHARLAAACDVKNDEQYQVLGTLIHGLEKIASSGLRAA
SDRAAAVADLSTVLDETRIVIEPALREAGISLIWEVGDALPLVQADHHSLLQVFVNLSRNSVRALEDRPVREVRISAEAE
RDLVVVRFRDSGPGVANPDELFRPFQAGAHSVGLGLYISRAILRAHGGGLRYDSGQGPGSCFTVELWPVENALEG
>Mature_475_residues
MMVTSAAMTRLDRFLAYSESHRLTVCMTAALWISLIAWWDSLLPDVSIGFLYLFPVLFSAAALNNLQILGVALLCGFLRK
IFDPLGDAPGAAERFGVAVASYAMTGFFVNALNQRRRLLVRHLDELERQIRLRKEAEQQVRILIETSPLAILTLNHSGQI
ALANESARNLFGFGDDSLQGADVSPYLPILNRMLHNARPGANLRTNVECKAHRQNGEVFLANVWLSTYRTSEGPGLAAVV
WDASENLRDREGAGFDSMMATSRVLIGAISHEIRNLASAAASAHARLAAACDVKNDEQYQVLGTLIHGLEKIASSGLRAA
SDRAAAVADLSTVLDETRIVIEPALREAGISLIWEVGDALPLVQADHHSLLQVFVNLSRNSVRALEDRPVREVRISAEAE
RDLVVVRFRDSGPGVANPDELFRPFQAGAHSVGLGLYISRAILRAHGGGLRYDSGQGPGSCFTVELWPVENALEG

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=213, Percent_Identity=30.0469483568075, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788549, Length=350, Percent_Identity=23.1428571428571, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51749; Mature: 51749

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVTSAAMTRLDRFLAYSESHRLTVCMTAALWISLIAWWDSLLPDVSIGFLYLFPVLFSA
CCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AALNNLQILGVALLCGFLRKIFDPLGDAPGAAERFGVAVASYAMTGFFVNALNQRRRLLV
HHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLDELERQIRLRKEAEQQVRILIETSPLAILTLNHSGQIALANESARNLFGFGDDSLQG
HHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCC
ADVSPYLPILNRMLHNARPGANLRTNVECKAHRQNGEVFLANVWLSTYRTSEGPGLAAVV
CCCCCHHHHHHHHHHCCCCCCCCEECCEEEEECCCCCEEEEEEEHHHHCCCCCCCEEEEE
WDASENLRDREGAGFDSMMATSRVLIGAISHEIRNLASAAASAHARLAAACDVKNDEQYQ
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VLGTLIHGLEKIASSGLRAASDRAAAVADLSTVLDETRIVIEPALREAGISLIWEVGDAL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHCCHHEEEHHCCCC
PLVQADHHSLLQVFVNLSRNSVRALEDRPVREVRISAEAERDLVVVRFRDSGPGVANPDE
CCCCCCHHHHHHHHHHHCCCHHHHHHCCCCHHEEECCCCCCCEEEEEEECCCCCCCCHHH
LFRPFQAGAHSVGLGLYISRAILRAHGGGLRYDSGQGPGSCFTVELWPVENALEG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MMVTSAAMTRLDRFLAYSESHRLTVCMTAALWISLIAWWDSLLPDVSIGFLYLFPVLFSA
CCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AALNNLQILGVALLCGFLRKIFDPLGDAPGAAERFGVAVASYAMTGFFVNALNQRRRLLV
HHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLDELERQIRLRKEAEQQVRILIETSPLAILTLNHSGQIALANESARNLFGFGDDSLQG
HHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCC
ADVSPYLPILNRMLHNARPGANLRTNVECKAHRQNGEVFLANVWLSTYRTSEGPGLAAVV
CCCCCHHHHHHHHHHCCCCCCCCEECCEEEEECCCCCEEEEEEEHHHHCCCCCCCEEEEE
WDASENLRDREGAGFDSMMATSRVLIGAISHEIRNLASAAASAHARLAAACDVKNDEQYQ
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VLGTLIHGLEKIASSGLRAASDRAAAVADLSTVLDETRIVIEPALREAGISLIWEVGDAL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHCCHHEEEHHCCCC
PLVQADHHSLLQVFVNLSRNSVRALEDRPVREVRISAEAERDLVVVRFRDSGPGVANPDE
CCCCCCHHHHHHHHHHHCCCHHHHHHCCCCHHEEECCCCCCCEEEEEEECCCCCCCCHHH
LFRPFQAGAHSVGLGLYISRAILRAHGGGLRYDSGQGPGSCFTVELWPVENALEG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]