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The map label for this gene is 116624010

Identifier: 116624010

GI number: 116624010

Start: 6214966

End: 6217119

Strand: Reverse

Name: 116624010

Synonym: Acid_4922

Alternate gene names: NA

Gene position: 6217119-6214966 (Counterclockwise)

Preceding gene: 116624011

Following gene: 116624007

Centisome position: 62.39

GC content: 63.0

Gene sequence:

>2154_bases
ATGAAAAAATTCGTTCTCCTTCTTGCCCTGGCCTGCGCCGCGTTGGCGGCCGATTCCGATCTCGCCCGCTGGAAGCAGCA
AGCCGCCGGCATCACCATCCTGCGCGACGATTGGGGCATTGCCCACGTCCACGGCAAGACCGACGCCGACGCCGTCTTCG
GCATGATCTACGCCCAGGCCGAGGACGATTTCAACCGCGTCGAGACCAACTACCTCAATTCCATGGGCCGCCTCGCCGAG
GCCGAAGGCGAATCGCGCATCTATCAGGACCTGCGAATGAAGCTCTTCATCGATCCCGCCGCGCTCAAGCAGCAGTACGC
CGCCAGCCCCGGCTGGCTCCAGGAACTGATGAATGCCTTCGCCGATGGCCTCAACTTTTATCTGGCCACCCACCCCAACA
CCAAACCGCGCGTGATCCAACATTTCGAACCCTGGATGGCGCTCTCTTTCACCGAGGGCAGCATCGGCGGCGATATCGAG
CGCATCAATCTGAACCAGCTCCAGGCCTTCTATGGTAAGACGCCCGTTGCCCAGTTGCCGCCGCCCGCCGACCCCTTCGC
CGAGCCCGAGCCCACCGGCTCCAACGGCATCGCCATCGCGCCCTCCAACACCGCCGGCAAGCACGCCCTGCTGCTCATCA
ATCCGCACACATCCTTCTTCTTCCGCTCTGAATTGCAGATGACCAGCGATCAGGGGCTCAACGCCTACGGCGCCGCCACC
TGGGGACAGTTCTTCATCTATCAGGGCTTCAACGAGCATGCCGGCTGGATGCACACTTCGAGCGGCGTCGATGCGGTGGA
CGAATATCTCGAAACCGTGACGAAGAGTGGCGATGGCTACACATACAAGTTTGGCGCCGAGCAGCGTCCGGTCACGGCCA
GTGAGATCGTGGTTCCCTACCGCACGCCGCAAGGCATGGCGGAGAAAAAGTTCACCGTCTACCGCACGCAGCACGGCCCC
ATCGTCCGCGAACTCAACGGCAAATGGGTCGCCATCCGCTTGATGCAGGAGCCGATCAAGGCGCTCACGCAGTCTTACAC
CCGCACCAAAGCGACCAATTACAAGGCCTATCTGGAGACCATGGAGCTCAAGGCGAACTCCTCCAACAACACGATCTACG
CCGACGATTCCGGCACCATCGCCTACTTCCACGGCAACTACATCCCGCGTCGCGACACCTCGTTCGATTGGACCAAGCCC
GTGGACGGCAGCAATCCCGCGACCGAGTACAAAGGCTTGCTCGAAGTCAACGAGGCGCCGAACCTGCTCAATCCCAAGGT
CGGCTGGCTCTACAACACCAACAACTGGCCCTGGTCCGCCGCCGGCCCCGACAGCCTGAAGAAGGAAAACTTCCCCGCCT
ACGTGGAAAGCGGCGGTGAATCCGCGCGCGGCCTCCACGCCATTCGCGTCCTCCAGAATCGCAAGGATTTCACGCTCGAT
TCCCTGATCGCCGCCGCCTACGATAGCTACCTTCCCTGGTTTGAGAAGCCCATGCCCGCGCTGATCAAAGCCTGGGACGA
AGCCACCGCCACTGACCCCTTGAAATCCAAACTCGCCGCGCAGATCGAGCTGCTTCGCAAATGGGACTACCGCTGGAACG
TGAACTCCGTGGCCACCTCGCTCGCCGTCTTCTGGGCCGAGGATCTCCGCCGCCGTGTCGGTGCAGGAGGCCGCGGCGGC
CGTGGCGACGATGCCATCGCTACCGCGCCCCCAGCGCAACTCCTGCAATCGCTCGACGCCGCCAGCGAAAAGATCGCCGC
GGATTTCGGCGGCTGGCAGACCAAGTGGGGCGACATCAATCGCTATCAACGCCTCACCGGCGACATCGTGCAGCCGTTCA
ATGACGCCGGCCCCAGCATCCCGGTCGGCTTCACGTCTTCGCAGTGGGGCTCGCTCGCCTCATTCGGCGCGCGCGCGTAT
CCCGGCACGAAGAAGTGGTATGGCACCAGCGGCAACAGCTTCGTCGCGGTTGTCGAGTTCGGCAAAACCGTCCGCGCCCG
CGCTGTCACGGCCGGCGGTGAAAGCGGCGACCCCAAGTCGCCCCACTTCAACGACCAGGCCGCACGCTACTCCACCGGCA
CCTTGCGCGAGGTCTATTTCTACCGCACCCAACTGCAGGGCCACACCCAGCGCGAATACCATCCGGGAGAGTGA

Upstream 100 bases:

>100_bases
GTGCAGCGCGGGCACGGCAACGTCACCAACATGACGGGGGGCATCGATCTGTGGTCCACTCAAGTGGATCCCACTGTTCC
CCGGTACTGAGATTATCCCC

Downstream 100 bases:

>100_bases
CGGCCTATTGTCGAATGCGAAGATTCACAGTCGGTACTGATGTGCCGTTCGCCCGGACAACGATGCCAACGTACCGAATG
GAGTCACCCATCGGCGTCCC

Product: peptidase S45, penicillin amidase

Products: NA

Alternate protein names: Glutaryl-7-ACA acylase; 7-beta-(4-carboxybutanamido)cephalosporanic acid acylase; CAD; GL-7-ACA acylase; GCA; Glutaryl-7-ACA acylase subunit alpha; Glutaryl-7-ACA acylase subunit beta [H]

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MKKFVLLLALACAALAADSDLARWKQQAAGITILRDDWGIAHVHGKTDADAVFGMIYAQAEDDFNRVETNYLNSMGRLAE
AEGESRIYQDLRMKLFIDPAALKQQYAASPGWLQELMNAFADGLNFYLATHPNTKPRVIQHFEPWMALSFTEGSIGGDIE
RINLNQLQAFYGKTPVAQLPPPADPFAEPEPTGSNGIAIAPSNTAGKHALLLINPHTSFFFRSELQMTSDQGLNAYGAAT
WGQFFIYQGFNEHAGWMHTSSGVDAVDEYLETVTKSGDGYTYKFGAEQRPVTASEIVVPYRTPQGMAEKKFTVYRTQHGP
IVRELNGKWVAIRLMQEPIKALTQSYTRTKATNYKAYLETMELKANSSNNTIYADDSGTIAYFHGNYIPRRDTSFDWTKP
VDGSNPATEYKGLLEVNEAPNLLNPKVGWLYNTNNWPWSAAGPDSLKKENFPAYVESGGESARGLHAIRVLQNRKDFTLD
SLIAAAYDSYLPWFEKPMPALIKAWDEATATDPLKSKLAAQIELLRKWDYRWNVNSVATSLAVFWAEDLRRRVGAGGRGG
RGDDAIATAPPAQLLQSLDAASEKIAADFGGWQTKWGDINRYQRLTGDIVQPFNDAGPSIPVGFTSSQWGSLASFGARAY
PGTKKWYGTSGNSFVAVVEFGKTVRARAVTAGGESGDPKSPHFNDQAARYSTGTLREVYFYRTQLQGHTQREYHPGE

Sequences:

>Translated_717_residues
MKKFVLLLALACAALAADSDLARWKQQAAGITILRDDWGIAHVHGKTDADAVFGMIYAQAEDDFNRVETNYLNSMGRLAE
AEGESRIYQDLRMKLFIDPAALKQQYAASPGWLQELMNAFADGLNFYLATHPNTKPRVIQHFEPWMALSFTEGSIGGDIE
RINLNQLQAFYGKTPVAQLPPPADPFAEPEPTGSNGIAIAPSNTAGKHALLLINPHTSFFFRSELQMTSDQGLNAYGAAT
WGQFFIYQGFNEHAGWMHTSSGVDAVDEYLETVTKSGDGYTYKFGAEQRPVTASEIVVPYRTPQGMAEKKFTVYRTQHGP
IVRELNGKWVAIRLMQEPIKALTQSYTRTKATNYKAYLETMELKANSSNNTIYADDSGTIAYFHGNYIPRRDTSFDWTKP
VDGSNPATEYKGLLEVNEAPNLLNPKVGWLYNTNNWPWSAAGPDSLKKENFPAYVESGGESARGLHAIRVLQNRKDFTLD
SLIAAAYDSYLPWFEKPMPALIKAWDEATATDPLKSKLAAQIELLRKWDYRWNVNSVATSLAVFWAEDLRRRVGAGGRGG
RGDDAIATAPPAQLLQSLDAASEKIAADFGGWQTKWGDINRYQRLTGDIVQPFNDAGPSIPVGFTSSQWGSLASFGARAY
PGTKKWYGTSGNSFVAVVEFGKTVRARAVTAGGESGDPKSPHFNDQAARYSTGTLREVYFYRTQLQGHTQREYHPGE
>Mature_717_residues
MKKFVLLLALACAALAADSDLARWKQQAAGITILRDDWGIAHVHGKTDADAVFGMIYAQAEDDFNRVETNYLNSMGRLAE
AEGESRIYQDLRMKLFIDPAALKQQYAASPGWLQELMNAFADGLNFYLATHPNTKPRVIQHFEPWMALSFTEGSIGGDIE
RINLNQLQAFYGKTPVAQLPPPADPFAEPEPTGSNGIAIAPSNTAGKHALLLINPHTSFFFRSELQMTSDQGLNAYGAAT
WGQFFIYQGFNEHAGWMHTSSGVDAVDEYLETVTKSGDGYTYKFGAEQRPVTASEIVVPYRTPQGMAEKKFTVYRTQHGP
IVRELNGKWVAIRLMQEPIKALTQSYTRTKATNYKAYLETMELKANSSNNTIYADDSGTIAYFHGNYIPRRDTSFDWTKP
VDGSNPATEYKGLLEVNEAPNLLNPKVGWLYNTNNWPWSAAGPDSLKKENFPAYVESGGESARGLHAIRVLQNRKDFTLD
SLIAAAYDSYLPWFEKPMPALIKAWDEATATDPLKSKLAAQIELLRKWDYRWNVNSVATSLAVFWAEDLRRRVGAGGRGG
RGDDAIATAPPAQLLQSLDAASEKIAADFGGWQTKWGDINRYQRLTGDIVQPFNDAGPSIPVGFTSSQWGSLASFGARAY
PGTKKWYGTSGNSFVAVVEFGKTVRARAVTAGGESGDPKSPHFNDQAARYSTGTLREVYFYRTQLQGHTQREYHPGE

Specific function: Catalyzes the deacylation of 7 beta-(4- carboxybutanamido)cephalosporanic acid (glutaryl-7- aminocephalosporanic acid or GL-7-ACA) to 7-aminocephalosporanic acid (7-ACA). Can not efficiently use cephalosporin C (CPC), penicillin G, or ampicillin as substr

COG id: COG2366

COG function: function code R; Protein related to penicillin acylase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S45 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014395
- InterPro:   IPR002692 [H]

Pfam domain/function: PF01804 Penicil_amidase [H]

EC number: =3.5.1.93 [H]

Molecular weight: Translated: 79418; Mature: 79418

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFVLLLALACAALAADSDLARWKQQAAGITILRDDWGIAHVHGKTDADAVFGMIYAQA
CHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCEEEECCCCCHHHHHHHHEEEC
EDDFNRVETNYLNSMGRLAEAEGESRIYQDLRMKLFIDPAALKQQYAASPGWLQELMNAF
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECHHHHHHHHCCCCHHHHHHHHHH
ADGLNFYLATHPNTKPRVIQHFEPWMALSFTEGSIGGDIERINLNQLQAFYGKTPVAQLP
HCCCEEEEEECCCCCCHHHHHCCCCEEEEECCCCCCCCEEEECHHHHHHHHCCCCCCCCC
PPADPFAEPEPTGSNGIAIAPSNTAGKHALLLINPHTSFFFRSELQMTSDQGLNAYGAAT
CCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCEEEHHHHHCCCCCCCCCCCCHH
WGQFFIYQGFNEHAGWMHTSSGVDAVDEYLETVTKSGDGYTYKFGAEQRPVTASEIVVPY
HCEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHEEEEE
RTPQGMAEKKFTVYRTQHGPIVRELNGKWVAIRLMQEPIKALTQSYTRTKATNYKAYLET
CCCCCCCCCCEEEEEECCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHH
MELKANSSNNTIYADDSGTIAYFHGNYIPRRDTSFDWTKPVDGSNPATEYKGLLEVNEAP
HHEEECCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCC
NLLNPKVGWLYNTNNWPWSAAGPDSLKKENFPAYVESGGESARGLHAIRVLQNRKDFTLD
CCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCHH
SLIAAAYDSYLPWFEKPMPALIKAWDEATATDPLKSKLAAQIELLRKWDYRWNVNSVATS
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LAVFWAEDLRRRVGAGGRGGRGDDAIATAPPAQLLQSLDAASEKIAADFGGWQTKWGDIN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
RYQRLTGDIVQPFNDAGPSIPVGFTSSQWGSLASFGARAYPGTKKWYGTSGNSFVAVVEF
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEC
GKTVRARAVTAGGESGDPKSPHFNDQAARYSTGTLREVYFYRTQLQGHTQREYHPGE
CCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKKFVLLLALACAALAADSDLARWKQQAAGITILRDDWGIAHVHGKTDADAVFGMIYAQA
CHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCEEEECCCCCHHHHHHHHEEEC
EDDFNRVETNYLNSMGRLAEAEGESRIYQDLRMKLFIDPAALKQQYAASPGWLQELMNAF
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECHHHHHHHHCCCCHHHHHHHHHH
ADGLNFYLATHPNTKPRVIQHFEPWMALSFTEGSIGGDIERINLNQLQAFYGKTPVAQLP
HCCCEEEEEECCCCCCHHHHHCCCCEEEEECCCCCCCCEEEECHHHHHHHHCCCCCCCCC
PPADPFAEPEPTGSNGIAIAPSNTAGKHALLLINPHTSFFFRSELQMTSDQGLNAYGAAT
CCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCEEEHHHHHCCCCCCCCCCCCHH
WGQFFIYQGFNEHAGWMHTSSGVDAVDEYLETVTKSGDGYTYKFGAEQRPVTASEIVVPY
HCEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHEEEEE
RTPQGMAEKKFTVYRTQHGPIVRELNGKWVAIRLMQEPIKALTQSYTRTKATNYKAYLET
CCCCCCCCCCEEEEEECCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHH
MELKANSSNNTIYADDSGTIAYFHGNYIPRRDTSFDWTKPVDGSNPATEYKGLLEVNEAP
HHEEECCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCC
NLLNPKVGWLYNTNNWPWSAAGPDSLKKENFPAYVESGGESARGLHAIRVLQNRKDFTLD
CCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCHH
SLIAAAYDSYLPWFEKPMPALIKAWDEATATDPLKSKLAAQIELLRKWDYRWNVNSVATS
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LAVFWAEDLRRRVGAGGRGGRGDDAIATAPPAQLLQSLDAASEKIAADFGGWQTKWGDIN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
RYQRLTGDIVQPFNDAGPSIPVGFTSSQWGSLASFGARAYPGTKKWYGTSGNSFVAVVEF
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEC
GKTVRARAVTAGGESGDPKSPHFNDQAARYSTGTLREVYFYRTQLQGHTQREYHPGE
CCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11080627 [H]