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The map label for this gene is glnA [H]

Identifier: 116623919

GI number: 116623919

Start: 6103963

End: 6105393

Strand: Direct

Name: glnA [H]

Synonym: Acid_4831

Alternate gene names: 116623919

Gene position: 6103963-6105393 (Clockwise)

Preceding gene: 116623918

Following gene: 116623920

Centisome position: 61.25

GC content: 59.19

Gene sequence:

>1431_bases
ATGGCAAACGAACTCCAAGACTTTCTGAAGCTTTCTTACACGGAACTGGAAGAGTTGAATCTCGAGGCGAAAGCCGACCG
GATGGGCCGCAAGCCCATGCACGAGATTCAGGAGAAGCGCCTTAAGTACCTCACGGACGAGAAGCGCATCAAGGCCGTCA
CGGTCCTTTTTAGCGATCTCGAAGGCCGTCTCCACATGCTCGATTACGACAAGAAGTTCCTTGTTAAGAGCTGGGACAAT
CTGACTTTTGACGGCTCCTCCATCCGCGGCTTCACCGCCCAGCGCGAGAGCGATCTTCGCCTGAATATGGATTGGAGCGC
CTTCTACTGGGCGCCTTCCGATATCTTCGGCGCCGGAAAGGTGCTGGTCTTCGGCGAGGTCATCGATAAAGACGGCACGC
CTTACAGCGCCGACATCCGCGGCGTGCTCAAGGCATACGCGCAGAGCCTTTTCGATAAACAGGGCTACACGCTGAACGCC
GCCAACGAAATCGAAGGCTTCCTTTTCAACGGCTCCGACGCCGAGCGCCGCTACCACGAAACCACGAAGTTCGATTACGT
CAACACCGGCGGCTACTATCACTCGCTGCCCGGCGACCCCCTGCGCAAATTCATCGACACTTGCGCCGAGGTGCAGCGCG
CCATGGGCTTCCAGAATGAAAAGGATCACCCTGAAGTCGCGCCCTCACAGTTCGAAATCAACTACAGCTATGCCGAAGTG
GTGGCCGCGGCGGACCAGATTCAGCTCTACAAGCTGATCTGCCGCCAGGTCGCGACCGGCATGGGGCTCACCGCCAGCTT
CCTGCCCAAACCCGTTGTCGGCGTCAACGGCAGCGGCATGCACACGAACGTCTCCATCACCAAGAACGGCAAGAACCTGT
TCTGGGATCCCTCCGGCCAGGAGAAGATGAGCAAGTTCGCATGGCAGTTCGTGGACCGCATTCTCACCCACGGCAACGAT
CTCTGCCTGCTGCTCAACGCCAGCGTCAACGCCTACCGCCGCCTCGATCCGCACTTCGAAGCTCCCAACCAGATCAAGGC
GTCGGCCACCGATCGCGGTTCCATGATCCGCATCCCGATCGGCAACGAGCGCAGCTCGCGCGTCGAAGTCCGCTCGGTGG
GGCCGGACGCCAACCCGTACATGGTGATGCTTTCCGTGTTCCGCACCGGGCTCGATGGCGAGACCGCGAAGATCAAGAAC
TTGCGCTCCGCCGAACGCTACCTGCCCGACAACGTCTATGACGCTCTGGCCAATTTTAAGAACGCGGAGTGGACCACCAA
ACTGCTCGGCGAAGATGTGAAGGGCCGTTATGCCGACCTGAAGCAATCTTCCGCCGACCGCTGTCCGCGGCTCCTGGGGA
CCTTCGTGAAGGCGCCCGAAGTGCAGTATCACCACGAAGTCTACAACCAGTTCCTCTGGAATATGTTCTAG

Upstream 100 bases:

>100_bases
ATCTATTGCCAACGCCGAAGAAACCGTTGCCAGAAGCGGTCCCGGTCATTCACAATCGATTATCCCCCGTCAGTAACGGA
AATATCGGATTGGAAGACTA

Downstream 100 bases:

>100_bases
GCTCAAAAAACACGCGGAGACGGGCCACCCCCGCCTCCGCGTTCCTATCGTGCTTCGCCCCCGTCGATGCGAAGACAACC
TCAACGGAGGCGGTCATGCT

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MANELQDFLKLSYTELEELNLEAKADRMGRKPMHEIQEKRLKYLTDEKRIKAVTVLFSDLEGRLHMLDYDKKFLVKSWDN
LTFDGSSIRGFTAQRESDLRLNMDWSAFYWAPSDIFGAGKVLVFGEVIDKDGTPYSADIRGVLKAYAQSLFDKQGYTLNA
ANEIEGFLFNGSDAERRYHETTKFDYVNTGGYYHSLPGDPLRKFIDTCAEVQRAMGFQNEKDHPEVAPSQFEINYSYAEV
VAAADQIQLYKLICRQVATGMGLTASFLPKPVVGVNGSGMHTNVSITKNGKNLFWDPSGQEKMSKFAWQFVDRILTHGND
LCLLLNASVNAYRRLDPHFEAPNQIKASATDRGSMIRIPIGNERSSRVEVRSVGPDANPYMVMLSVFRTGLDGETAKIKN
LRSAERYLPDNVYDALANFKNAEWTTKLLGEDVKGRYADLKQSSADRCPRLLGTFVKAPEVQYHHEVYNQFLWNMF

Sequences:

>Translated_476_residues
MANELQDFLKLSYTELEELNLEAKADRMGRKPMHEIQEKRLKYLTDEKRIKAVTVLFSDLEGRLHMLDYDKKFLVKSWDN
LTFDGSSIRGFTAQRESDLRLNMDWSAFYWAPSDIFGAGKVLVFGEVIDKDGTPYSADIRGVLKAYAQSLFDKQGYTLNA
ANEIEGFLFNGSDAERRYHETTKFDYVNTGGYYHSLPGDPLRKFIDTCAEVQRAMGFQNEKDHPEVAPSQFEINYSYAEV
VAAADQIQLYKLICRQVATGMGLTASFLPKPVVGVNGSGMHTNVSITKNGKNLFWDPSGQEKMSKFAWQFVDRILTHGND
LCLLLNASVNAYRRLDPHFEAPNQIKASATDRGSMIRIPIGNERSSRVEVRSVGPDANPYMVMLSVFRTGLDGETAKIKN
LRSAERYLPDNVYDALANFKNAEWTTKLLGEDVKGRYADLKQSSADRCPRLLGTFVKAPEVQYHHEVYNQFLWNMF
>Mature_475_residues
ANELQDFLKLSYTELEELNLEAKADRMGRKPMHEIQEKRLKYLTDEKRIKAVTVLFSDLEGRLHMLDYDKKFLVKSWDNL
TFDGSSIRGFTAQRESDLRLNMDWSAFYWAPSDIFGAGKVLVFGEVIDKDGTPYSADIRGVLKAYAQSLFDKQGYTLNAA
NEIEGFLFNGSDAERRYHETTKFDYVNTGGYYHSLPGDPLRKFIDTCAEVQRAMGFQNEKDHPEVAPSQFEINYSYAEVV
AAADQIQLYKLICRQVATGMGLTASFLPKPVVGVNGSGMHTNVSITKNGKNLFWDPSGQEKMSKFAWQFVDRILTHGNDL
CLLLNASVNAYRRLDPHFEAPNQIKASATDRGSMIRIPIGNERSSRVEVRSVGPDANPYMVMLSVFRTGLDGETAKIKNL
RSAERYLPDNVYDALANFKNAEWTTKLLGEDVKGRYADLKQSSADRCPRLLGTFVKAPEVQYHHEVYNQFLWNMF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=402, Percent_Identity=32.089552238806, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI87081870, Length=344, Percent_Identity=29.3604651162791, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 54109; Mature: 53978

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANELQDFLKLSYTELEELNLEAKADRMGRKPMHEIQEKRLKYLTDEKRIKAVTVLFSDL
CCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
EGRLHMLDYDKKFLVKSWDNLTFDGSSIRGFTAQRESDLRLNMDWSAFYWAPSDIFGAGK
CCCEEEECCCHHHHHHCCCCCEECCCCCCCCCCCCCCCCEEECCCCEEEECCHHHCCCCE
VLVFGEVIDKDGTPYSADIRGVLKAYAQSLFDKQGYTLNAANEIEGFLFNGSDAERRYHE
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCCHHHHHHH
TTKFDYVNTGGYYHSLPGDPLRKFIDTCAEVQRAMGFQNEKDHPEVAPSQFEINYSYAEV
CCCCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHH
VAAADQIQLYKLICRQVATGMGLTASFLPKPVVGVNGSGMHTNVSITKNGKNLFWDPSGQ
HHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCEEEEEEEECCCEEEECCCCH
EKMSKFAWQFVDRILTHGNDLCLLLNASVNAYRRLDPHFEAPNQIKASATDRGSMIRIPI
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCCCEECCCCCCCCEEEEEC
GNERSSRVEVRSVGPDANPYMVMLSVFRTGLDGETAKIKNLRSAERYLPDNVYDALANFK
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC
NAEWTTKLLGEDVKGRYADLKQSSADRCPRLLGTFVKAPEVQYHHEVYNQFLWNMF
CCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANELQDFLKLSYTELEELNLEAKADRMGRKPMHEIQEKRLKYLTDEKRIKAVTVLFSDL
CHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
EGRLHMLDYDKKFLVKSWDNLTFDGSSIRGFTAQRESDLRLNMDWSAFYWAPSDIFGAGK
CCCEEEECCCHHHHHHCCCCCEECCCCCCCCCCCCCCCCEEECCCCEEEECCHHHCCCCE
VLVFGEVIDKDGTPYSADIRGVLKAYAQSLFDKQGYTLNAANEIEGFLFNGSDAERRYHE
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCCHHHHHHH
TTKFDYVNTGGYYHSLPGDPLRKFIDTCAEVQRAMGFQNEKDHPEVAPSQFEINYSYAEV
CCCCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHH
VAAADQIQLYKLICRQVATGMGLTASFLPKPVVGVNGSGMHTNVSITKNGKNLFWDPSGQ
HHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCEEEEEEEECCCEEEECCCCH
EKMSKFAWQFVDRILTHGNDLCLLLNASVNAYRRLDPHFEAPNQIKASATDRGSMIRIPI
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCCCEECCCCCCCCEEEEEC
GNERSSRVEVRSVGPDANPYMVMLSVFRTGLDGETAKIKNLRSAERYLPDNVYDALANFK
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC
NAEWTTKLLGEDVKGRYADLKQSSADRCPRLLGTFVKAPEVQYHHEVYNQFLWNMF
CCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2572584 [H]