Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 116623849

GI number: 116623849

Start: 6024095

End: 6025399

Strand: Reverse

Name: gdhA [H]

Synonym: Acid_4761

Alternate gene names: 116623849

Gene position: 6025399-6024095 (Counterclockwise)

Preceding gene: 116623851

Following gene: 116623848

Centisome position: 60.46

GC content: 62.15

Gene sequence:

>1305_bases
ATGAGCTTCGGAGGCTCGAGAGTGGCCACGTCGACATTGCCTGTTTCCATTTCCCTCGAAAACGAAAAGAACCCCTGGCT
CGCCGCCGCCGCGCGCTTTGAAGAAGCCGCGAAGCGGCTCAAGCTGGACGACGGTATGCGTAAAGTGCTCGGCACCCCGT
CCAAGGAACTCACCGTTCACATCCCCGTGCAGCTCGATGACGGCCGGATCGAAGTCTTCACCGGATACCGCGTTCAGCAT
TCCGTCGCTCGCGGCCCGGCCAAGGGCGGTATCCGCTTCGCGCCGGACGTCACGCTCGATGAAGTCCGCGCCCTCGCCTC
CTGGATGACCTGGAAGTGCGCCGTCGTCAACATCCCCTTCGGCGGCGGCAAAGGCGGCGTCATCTGCGATCCGCACATCC
TCTCCGATACCGAGCTCGAAAAACTCACCCGCCGGTACACCGCCGAGATCATCGATTTCATAGGACCGGAGCGCGACGTG
CCCGCCCCGGATGTCAACACCAACGAAAAGGTCATGGCATGGATGATGGACACCTATTCCATGCACGCGCGCCACACGGT
CACCGCCATAGTCACCGGAAAACCCATGGCGCTCGGCGGGTCCCGCGGCCGTCCCGAAGCTACCGGACGCGGCTGCATGA
TGGTCACCCAGCGCGCTCTGAACCGCATGGGCAAACGCCCCGAAGATACCTCCGTCGTCATTCAGGGCTTCGGCAATGTC
GGCGGCATGGCGGCCAAACTGATGAGCGCCGTCGGCTTCAAGATCATCGCTATTGTCGAGTATGATGGCGCCGCCTACAA
TCCCAATGGCCTCGATATCGCCGCGCTCCAGCTTCATCGGAAGGAGACGGGTTCCATCACGGGCTTCTCCGGCGGCGAAG
ATATGGATAAGACCGAGGCCATGTTCCTCGAATGCGACGTCCTCATCCCTGCCGCCACCGAGAACGTCATCACCTCCCAG
AACGCGCACCGCGTGCGCTGCAGGATCCTCTGCGAAGGCGCCAACGGTCCCACTACTCCGCTGGCCGACGATATTCTCGC
CGAGAAGAAGGTCTTCGTGATCCCCGACATCCTCGCCAACGCCGGTGGCGTCACCGTCTCCTACTTCGAGTGGGTGCAGG
ACCGCCAGGGCTTCTTCTGGAACGAACAGCTCGTCAACGAACGCCTCCAGGAGATCATGGACGAAAGCTTCGACGCCATC
GTCGCCTATGCTGAGGCCCACCAGGTTAACAATCGCATCGCCGCCTACATGGTCGCCCTCGATCGCGTTGCCCAGGCCAT
CAAACTCCGCGGCATCTACGCATAG

Upstream 100 bases:

>100_bases
CCCGTTTTTGCTGCTCGACTCTTACTTAAGCCATTTCTCATCAGCCGATCAATCCGCAGAATTGGGTACAAATAGCCCGT
TTTTCGTGCGGGGTGTAATA

Downstream 100 bases:

>100_bases
GCGATCTCTCGCAAACCGCGACCGTCTGGGAGCGATCTAAGATGCGACACTGTACCTAGCGAATGTCCGACGCCTCCACG
CCGCTGATGCGGCAGTACAA

Product: Glu/Leu/Phe/Val dehydrogenase, C terminal

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSFGGSRVATSTLPVSISLENEKNPWLAAAARFEEAAKRLKLDDGMRKVLGTPSKELTVHIPVQLDDGRIEVFTGYRVQH
SVARGPAKGGIRFAPDVTLDEVRALASWMTWKCAVVNIPFGGGKGGVICDPHILSDTELEKLTRRYTAEIIDFIGPERDV
PAPDVNTNEKVMAWMMDTYSMHARHTVTAIVTGKPMALGGSRGRPEATGRGCMMVTQRALNRMGKRPEDTSVVIQGFGNV
GGMAAKLMSAVGFKIIAIVEYDGAAYNPNGLDIAALQLHRKETGSITGFSGGEDMDKTEAMFLECDVLIPAATENVITSQ
NAHRVRCRILCEGANGPTTPLADDILAEKKVFVIPDILANAGGVTVSYFEWVQDRQGFFWNEQLVNERLQEIMDESFDAI
VAYAEAHQVNNRIAAYMVALDRVAQAIKLRGIYA

Sequences:

>Translated_434_residues
MSFGGSRVATSTLPVSISLENEKNPWLAAAARFEEAAKRLKLDDGMRKVLGTPSKELTVHIPVQLDDGRIEVFTGYRVQH
SVARGPAKGGIRFAPDVTLDEVRALASWMTWKCAVVNIPFGGGKGGVICDPHILSDTELEKLTRRYTAEIIDFIGPERDV
PAPDVNTNEKVMAWMMDTYSMHARHTVTAIVTGKPMALGGSRGRPEATGRGCMMVTQRALNRMGKRPEDTSVVIQGFGNV
GGMAAKLMSAVGFKIIAIVEYDGAAYNPNGLDIAALQLHRKETGSITGFSGGEDMDKTEAMFLECDVLIPAATENVITSQ
NAHRVRCRILCEGANGPTTPLADDILAEKKVFVIPDILANAGGVTVSYFEWVQDRQGFFWNEQLVNERLQEIMDESFDAI
VAYAEAHQVNNRIAAYMVALDRVAQAIKLRGIYA
>Mature_433_residues
SFGGSRVATSTLPVSISLENEKNPWLAAAARFEEAAKRLKLDDGMRKVLGTPSKELTVHIPVQLDDGRIEVFTGYRVQHS
VARGPAKGGIRFAPDVTLDEVRALASWMTWKCAVVNIPFGGGKGGVICDPHILSDTELEKLTRRYTAEIIDFIGPERDVP
APDVNTNEKVMAWMMDTYSMHARHTVTAIVTGKPMALGGSRGRPEATGRGCMMVTQRALNRMGKRPEDTSVVIQGFGNVG
GMAAKLMSAVGFKIIAIVEYDGAAYNPNGLDIAALQLHRKETGSITGFSGGEDMDKTEAMFLECDVLIPAATENVITSQN
AHRVRCRILCEGANGPTTPLADDILAEKKVFVIPDILANAGGVTVSYFEWVQDRQGFFWNEQLVNERLQEIMDESFDAIV
AYAEAHQVNNRIAAYMVALDRVAQAIKLRGIYA

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=343, Percent_Identity=46.9387755102041, Blast_Score=315, Evalue=6e-86,
Organism=Homo sapiens, GI4885281, Length=356, Percent_Identity=46.0674157303371, Blast_Score=311, Evalue=8e-85,
Organism=Escherichia coli, GI1788059, Length=381, Percent_Identity=31.2335958005249, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17544676, Length=401, Percent_Identity=41.3965087281795, Blast_Score=290, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6324951, Length=415, Percent_Identity=27.710843373494, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319256, Length=391, Percent_Identity=28.9002557544757, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI45553475, Length=397, Percent_Identity=41.3098236775819, Blast_Score=308, Evalue=4e-84,
Organism=Drosophila melanogaster, GI45549226, Length=367, Percent_Identity=42.7792915531335, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24649283, Length=402, Percent_Identity=39.5522388059701, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=44.8028673835125, Blast_Score=252, Evalue=4e-67,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 47333; Mature: 47202

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFGGSRVATSTLPVSISLENEKNPWLAAAARFEEAAKRLKLDDGMRKVLGTPSKELTVH
CCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEE
IPVQLDDGRIEVFTGYRVQHSVARGPAKGGIRFAPDVTLDEVRALASWMTWKCAVVNIPF
EEEEECCCEEEEEECCEEEHHHHCCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEEEECC
GGGKGGVICDPHILSDTELEKLTRRYTAEIIDFIGPERDVPAPDVNTNEKVMAWMMDTYS
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHH
MHARHTVTAIVTGKPMALGGSRGRPEATGRGCMMVTQRALNRMGKRPEDTSVVIQGFGNV
HCCCEEEEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCC
GGMAAKLMSAVGFKIIAIVEYDGAAYNPNGLDIAALQLHRKETGSITGFSGGEDMDKTEA
CHHHHHHHHHCCEEEEEEEEECCCEECCCCCEEEEEEEEHHCCCCEECCCCCCCCCCHHE
MFLECDVLIPAATENVITSQNAHRVRCRILCEGANGPTTPLADDILAEKKVFVIPDILAN
EEEEEEEEEECCCCHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHCCCEEEECHHHHC
AGGVTVSYFEWVQDRQGFFWNEQLVNERLQEIMDESFDAIVAYAEAHQVNNRIAAYMVAL
CCCCEEHHHHHHHHCCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DRVAQAIKLRGIYA
HHHHHHHHHCCCCC
>Mature Secondary Structure 
SFGGSRVATSTLPVSISLENEKNPWLAAAARFEEAAKRLKLDDGMRKVLGTPSKELTVH
CCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEE
IPVQLDDGRIEVFTGYRVQHSVARGPAKGGIRFAPDVTLDEVRALASWMTWKCAVVNIPF
EEEEECCCEEEEEECCEEEHHHHCCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEEEECC
GGGKGGVICDPHILSDTELEKLTRRYTAEIIDFIGPERDVPAPDVNTNEKVMAWMMDTYS
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHH
MHARHTVTAIVTGKPMALGGSRGRPEATGRGCMMVTQRALNRMGKRPEDTSVVIQGFGNV
HCCCEEEEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCC
GGMAAKLMSAVGFKIIAIVEYDGAAYNPNGLDIAALQLHRKETGSITGFSGGEDMDKTEA
CHHHHHHHHHCCEEEEEEEEECCCEECCCCCEEEEEEEEHHCCCCEECCCCCCCCCCHHE
MFLECDVLIPAATENVITSQNAHRVRCRILCEGANGPTTPLADDILAEKKVFVIPDILAN
EEEEEEEEEECCCCHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHCCCEEEECHHHHC
AGGVTVSYFEWVQDRQGFFWNEQLVNERLQEIMDESFDAIVAYAEAHQVNNRIAAYMVAL
CCCCEEHHHHHHHHCCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DRVAQAIKLRGIYA
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]