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The map label for this gene is pfp [H]

Identifier: 116623827

GI number: 116623827

Start: 5997985

End: 5999169

Strand: Reverse

Name: pfp [H]

Synonym: Acid_4739

Alternate gene names: 116623827

Gene position: 5999169-5997985 (Counterclockwise)

Preceding gene: 116623828

Following gene: 116623826

Centisome position: 60.2

GC content: 61.6

Gene sequence:

>1185_bases
ATGGCAGGCAAGCGTATCGGCATACTCACGGGCGGCGGCGATGTTCCGGGACTGAATTCCGTGATCAAAACGGTGGTATA
CCGTGGAAGCGAAATCGGCTGCGAGGTGATCGGAATTCGCCGCGGCTGGGAAGGGCTGACCCACGTCAACCTGCAGGATC
CGGCGAGCCGCACCCGCTACGTGCTGCCGTTGAATCGCGAGAATACCCGCACCATCGACCGCACCGGCGGTACCTACCTC
CACAGTTCGCGCACCAACCCCTCCAAAATGAAGAAGCTGCCCCCGGTGCTCGAAGGGCAGGATTTCCCCAAGGTCACGTC
CACCAAGAAGGGCGTCACTTCCGAAGTCTTCGACGTCTCCAAGGCCGTCCTGAAGAACATCGAAACCTTGAACCTGGATT
ACCTGATCGCGATCGGCGGCGACGACACGCTGAGCTATGCCGCCGAGTTGGACCGCCAGGGCTTCCGCGTAATCGCCGTG
CCCAAAACGATGGATAACGACGTGCAGAATACCGAGTACTGCATCGGTTTCTCCACCGCCATCACCCGCGCCATGGATGC
CATCGAGCGCCAGCGCACCACCGTCGGCTCGCACGAGCGCATCGGCCTCTTCCGCGTCTTCGGCCGCGACGCCGGCTATA
CTGCCCTCTACACCGCGTATGTCACCTCCGTTCGCTGCTGCATTCCCGAGCACAAGGTGGACCTCCCCAAGCTGATCGAT
CTCCTGGTCACCGACAAGAAGAACAATCCGAGCAACTACTCCCTCGCCATTCTGAGCGAAGGCGCCGAGTGGGTTGGTTA
CACCGTGAAGGAATACGGGGAAGCCGACGCTTTCGGACACCGCAAGAAGATGAGCGTGGCCGAAGACCTCAGCAATGAGA
TCAAGAACTCCACCGGCGAGGAGACCATCGTCAGCGACCTGACTTACGACCTCCGCTCCGGCAGCGCCGATTTCGTGGAC
CGCATGGTCGCCTCTACCTTCGCCGGCATGGCCATGGACGCCATCCAGGAGGGCAAACACGGCCTCATGACCGCGATCTC
CAACGGCAGCTACACCATGGTCGGTATCCCCGACCCCAAACTCGGCCCGCGCCGCGTCGATGTCGCCTCCATGTACAACA
CCGACCGCTACCGCCCCGTCTACGATCACAAACTGGGCCTGCCGATCTTCCTGACGCGCGCCTAA

Upstream 100 bases:

>100_bases
GGGAGACCCGCTTTTGCTTTCACGGGCGAAATCGCACGCCATCCTTTATAATGAGATTTTCCAATCTGAAAGCACCCTTA
CTTAACCAGAGGAGTCATTG

Downstream 100 bases:

>100_bases
ATGCTAAAAGCCTCTCGCAGAGACGCAGAGCCGCAGAGAACCGCAGAGAAGAATTGGCCCTTGTCTTCTTTGCGTTACTT
TGCGGCTTTGCGGCTTTGCG

Product: pyrophosphate-dependent phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAGKRIGILTGGGDVPGLNSVIKTVVYRGSEIGCEVIGIRRGWEGLTHVNLQDPASRTRYVLPLNRENTRTIDRTGGTYL
HSSRTNPSKMKKLPPVLEGQDFPKVTSTKKGVTSEVFDVSKAVLKNIETLNLDYLIAIGGDDTLSYAAELDRQGFRVIAV
PKTMDNDVQNTEYCIGFSTAITRAMDAIERQRTTVGSHERIGLFRVFGRDAGYTALYTAYVTSVRCCIPEHKVDLPKLID
LLVTDKKNNPSNYSLAILSEGAEWVGYTVKEYGEADAFGHRKKMSVAEDLSNEIKNSTGEETIVSDLTYDLRSGSADFVD
RMVASTFAGMAMDAIQEGKHGLMTAISNGSYTMVGIPDPKLGPRRVDVASMYNTDRYRPVYDHKLGLPIFLTRA

Sequences:

>Translated_394_residues
MAGKRIGILTGGGDVPGLNSVIKTVVYRGSEIGCEVIGIRRGWEGLTHVNLQDPASRTRYVLPLNRENTRTIDRTGGTYL
HSSRTNPSKMKKLPPVLEGQDFPKVTSTKKGVTSEVFDVSKAVLKNIETLNLDYLIAIGGDDTLSYAAELDRQGFRVIAV
PKTMDNDVQNTEYCIGFSTAITRAMDAIERQRTTVGSHERIGLFRVFGRDAGYTALYTAYVTSVRCCIPEHKVDLPKLID
LLVTDKKNNPSNYSLAILSEGAEWVGYTVKEYGEADAFGHRKKMSVAEDLSNEIKNSTGEETIVSDLTYDLRSGSADFVD
RMVASTFAGMAMDAIQEGKHGLMTAISNGSYTMVGIPDPKLGPRRVDVASMYNTDRYRPVYDHKLGLPIFLTRA
>Mature_393_residues
AGKRIGILTGGGDVPGLNSVIKTVVYRGSEIGCEVIGIRRGWEGLTHVNLQDPASRTRYVLPLNRENTRTIDRTGGTYLH
SSRTNPSKMKKLPPVLEGQDFPKVTSTKKGVTSEVFDVSKAVLKNIETLNLDYLIAIGGDDTLSYAAELDRQGFRVIAVP
KTMDNDVQNTEYCIGFSTAITRAMDAIERQRTTVGSHERIGLFRVFGRDAGYTALYTAYVTSVRCCIPEHKVDLPKLIDL
LVTDKKNNPSNYSLAILSEGAEWVGYTVKEYGEADAFGHRKKMSVAEDLSNEIKNSTGEETIVSDLTYDLRSGSADFVDR
MVASTFAGMAMDAIQEGKHGLMTAISNGSYTMVGIPDPKLGPRRVDVASMYNTDRYRPVYDHKLGLPIFLTRA

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=364, Percent_Identity=24.1758241758242, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI266453768, Length=368, Percent_Identity=25, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI266453748, Length=368, Percent_Identity=25, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI4505749, Length=368, Percent_Identity=25, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI266453619, Length=368, Percent_Identity=25, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI48762920, Length=360, Percent_Identity=25, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1790350, Length=346, Percent_Identity=28.6127167630058, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25147584, Length=344, Percent_Identity=22.6744186046512, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321679, Length=263, Percent_Identity=24.7148288973384, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17647809, Length=402, Percent_Identity=24.1293532338308, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652337, Length=402, Percent_Identity=24.1293532338308, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28573326, Length=402, Percent_Identity=23.8805970149254, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 43355; Mature: 43224

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKRIGILTGGGDVPGLNSVIKTVVYRGSEIGCEVIGIRRGWEGLTHVNLQDPASRTRY
CCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCCCCEE
VLPLNRENTRTIDRTGGTYLHSSRTNPSKMKKLPPVLEGQDFPKVTSTKKGVTSEVFDVS
EEEECCCCCEEEECCCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KAVLKNIETLNLDYLIAIGGDDTLSYAAELDRQGFRVIAVPKTMDNDVQNTEYCIGFSTA
HHHHHCCCEECEEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEHHHH
ITRAMDAIERQRTTVGSHERIGLFRVFGRDAGYTALYTAYVTSVRCCIPEHKVDLPKLID
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LLVTDKKNNPSNYSLAILSEGAEWVGYTVKEYGEADAFGHRKKMSVAEDLSNEIKNSTGE
HHHCCCCCCCCCEEEEEEECCCHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
ETIVSDLTYDLRSGSADFVDRMVASTFAGMAMDAIQEGKHGLMTAISNGSYTMVGIPDPK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCC
LGPRRVDVASMYNTDRYRPVYDHKLGLPIFLTRA
CCCCEEEHHHHHCCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
AGKRIGILTGGGDVPGLNSVIKTVVYRGSEIGCEVIGIRRGWEGLTHVNLQDPASRTRY
CCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCCCCEE
VLPLNRENTRTIDRTGGTYLHSSRTNPSKMKKLPPVLEGQDFPKVTSTKKGVTSEVFDVS
EEEECCCCCEEEECCCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KAVLKNIETLNLDYLIAIGGDDTLSYAAELDRQGFRVIAVPKTMDNDVQNTEYCIGFSTA
HHHHHCCCEECEEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEHHHH
ITRAMDAIERQRTTVGSHERIGLFRVFGRDAGYTALYTAYVTSVRCCIPEHKVDLPKLID
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LLVTDKKNNPSNYSLAILSEGAEWVGYTVKEYGEADAFGHRKKMSVAEDLSNEIKNSTGE
HHHCCCCCCCCCEEEEEEECCCHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
ETIVSDLTYDLRSGSADFVDRMVASTFAGMAMDAIQEGKHGLMTAISNGSYTMVGIPDPK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCC
LGPRRVDVASMYNTDRYRPVYDHKLGLPIFLTRA
CCCCEEEHHHHHCCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]