Click here to switch to the map view.

The map label for this gene is lipA [H]

Identifier: 116623793

GI number: 116623793

Start: 5957599

End: 5958471

Strand: Direct

Name: lipA [H]

Synonym: Acid_4705

Alternate gene names: 116623793

Gene position: 5957599-5958471 (Clockwise)

Preceding gene: 116623792

Following gene: 116623794

Centisome position: 59.78

GC content: 65.86

Gene sequence:

>873_bases
ATGGAATCAGTGCTGGTTTCCATTTCCCCCAAGCTCGATCGTCCGCAGTGGCTGCGTGCGCCCGCACCCGCAGGCGAGAA
TTATCGCGATCTCAAACAGTTGATCGATCGCCTGCGCCTGCACACCGTCTGCGAAAGCGCCGCCTGTCCCAACGTCGGCG
AATGCTGGAATCATCGCACCGCGACCTTCATGATGCTGGGCAATGTCTGCACCCGCCGCTGCGGCTTCTGCGCCGTGCAG
AAGGGCGCGCCCCTGCCGGTGGACTACGACGAGCCCTCGCGAATCGCCGAGGCCGTGGCCGCGATGGGCCTCAAGTTCGC
CGTGATCACCAGCGTCAATCGCGATGATCGCCGCGATGGCGGCGCGGAACTCTTCGCTATGGTGATCCGCGCGATTCGCG
ACCGCGTGCCCGGTTGCGGCGTGGAAGTGCTGATCCCCGATTTCCAGGGCAATCGCGAGGCCGTGGAGACCGTCATGGAA
GCGCGTCCCGACGTGCTCAATCACAATACCGAGACTGTGCCCCGCCTCTACCGGCAGGTGCGCCTCGGCGCGCGCTACGA
GCGCTCGCTCGACGTGCTCGCCCACGCCAAGCGCGTGCAGCCCGCCACTCCGACCAAGTCCGGCCTCATGCTCGGGCTCG
GCGAAACCGCCGACGAAGTCTTGCAGGTAATGCGCGATCTCCGCGCCCACAACGTCGATATCCTGACGCTGGGTCAATAC
CTGAGGCCGTCGCCCAAGCACCTGCCCATCGTCCGCTACGTAACTCCCGCGGAGTTCGAAGAGTTCCGCGACGCGGGCCG
CGCCATGGGCTTCGCCCACGTGGAATCCGGCCCCCTGGTCCGCAGTTCCTATCACGCCTCCAACGCCGTGTAG

Upstream 100 bases:

>100_bases
CGCGAGAACGGCATTCCGATCGCCGGCGGCAATTGGGACCAGCTGGTGAAGGCGGCCGCGAAGGTCCACGTGTCGGCTCC
CGCGGTCTGATCCCGCTACA

Downstream 100 bases:

>100_bases
GCCTGCTCTTGTTTGCTCGCAGCCCGCACACATCCGCACCCTAAACTTCGAATCGTGACCGCCTTGTGGGGCAGCCAATC
CTGGCTGCAGCCGCCTTTCA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MESVLVSISPKLDRPQWLRAPAPAGENYRDLKQLIDRLRLHTVCESAACPNVGECWNHRTATFMMLGNVCTRRCGFCAVQ
KGAPLPVDYDEPSRIAEAVAAMGLKFAVITSVNRDDRRDGGAELFAMVIRAIRDRVPGCGVEVLIPDFQGNREAVETVME
ARPDVLNHNTETVPRLYRQVRLGARYERSLDVLAHAKRVQPATPTKSGLMLGLGETADEVLQVMRDLRAHNVDILTLGQY
LRPSPKHLPIVRYVTPAEFEEFRDAGRAMGFAHVESGPLVRSSYHASNAV

Sequences:

>Translated_290_residues
MESVLVSISPKLDRPQWLRAPAPAGENYRDLKQLIDRLRLHTVCESAACPNVGECWNHRTATFMMLGNVCTRRCGFCAVQ
KGAPLPVDYDEPSRIAEAVAAMGLKFAVITSVNRDDRRDGGAELFAMVIRAIRDRVPGCGVEVLIPDFQGNREAVETVME
ARPDVLNHNTETVPRLYRQVRLGARYERSLDVLAHAKRVQPATPTKSGLMLGLGETADEVLQVMRDLRAHNVDILTLGQY
LRPSPKHLPIVRYVTPAEFEEFRDAGRAMGFAHVESGPLVRSSYHASNAV
>Mature_290_residues
MESVLVSISPKLDRPQWLRAPAPAGENYRDLKQLIDRLRLHTVCESAACPNVGECWNHRTATFMMLGNVCTRRCGFCAVQ
KGAPLPVDYDEPSRIAEAVAAMGLKFAVITSVNRDDRRDGGAELFAMVIRAIRDRVPGCGVEVLIPDFQGNREAVETVME
ARPDVLNHNTETVPRLYRQVRLGARYERSLDVLAHAKRVQPATPTKSGLMLGLGETADEVLQVMRDLRAHNVDILTLGQY
LRPSPKHLPIVRYVTPAEFEEFRDAGRAMGFAHVESGPLVRSSYHASNAV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=283, Percent_Identity=46.9964664310954, Blast_Score=265, Evalue=3e-71,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=46.9635627530364, Blast_Score=228, Evalue=7e-60,
Organism=Escherichia coli, GI1786846, Length=277, Percent_Identity=53.0685920577617, Blast_Score=308, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI32564533, Length=252, Percent_Identity=46.031746031746, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=282, Percent_Identity=44.6808510638298, Blast_Score=249, Evalue=4e-67,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=46.9964664310954, Blast_Score=266, Evalue=9e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 32210; Mature: 32210

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESVLVSISPKLDRPQWLRAPAPAGENYRDLKQLIDRLRLHTVCESAACPNVGECWNHRT
CCCEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCH
ATFMMLGNVCTRRCGFCAVQKGAPLPVDYDEPSRIAEAVAAMGLKFAVITSVNRDDRRDG
HHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCH
GAELFAMVIRAIRDRVPGCGVEVLIPDFQGNREAVETVMEARPDVLNHNTETVPRLYRQV
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHH
RLGARYERSLDVLAHAKRVQPATPTKSGLMLGLGETADEVLQVMRDLRAHNVDILTLGQY
HHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCHH
LRPSPKHLPIVRYVTPAEFEEFRDAGRAMGFAHVESGPLVRSSYHASNAV
HCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MESVLVSISPKLDRPQWLRAPAPAGENYRDLKQLIDRLRLHTVCESAACPNVGECWNHRT
CCCEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCH
ATFMMLGNVCTRRCGFCAVQKGAPLPVDYDEPSRIAEAVAAMGLKFAVITSVNRDDRRDG
HHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCH
GAELFAMVIRAIRDRVPGCGVEVLIPDFQGNREAVETVMEARPDVLNHNTETVPRLYRQV
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHH
RLGARYERSLDVLAHAKRVQPATPTKSGLMLGLGETADEVLQVMRDLRAHNVDILTLGQY
HHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCHH
LRPSPKHLPIVRYVTPAEFEEFRDAGRAMGFAHVESGPLVRSSYHASNAV
HCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA