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The map label for this gene is amsH [H]

Identifier: 116623726

GI number: 116623726

Start: 5866541

End: 5867593

Strand: Direct

Name: amsH [H]

Synonym: Acid_4638

Alternate gene names: 116623726

Gene position: 5866541-5867593 (Clockwise)

Preceding gene: 116623725

Following gene: 116623727

Centisome position: 58.87

GC content: 57.93

Gene sequence:

>1053_bases
ATGAAAATAACAATGAAGATCCCAAGCTTGATGCTGATTGGAGTTGCGGTGCATGTGTCTGCACTCGTGGCCCAAGTGCC
TCCGGCACAACCTGGAGGTCAGCCGCGGCAGCAGCTGCCGGCGTCGCAGCAGTCGGTGAATGTGGCTTCGGCTCCGATGG
AGCGTCTTCGGCCCAACTACGTGCTGCGGCCGGGAGACCAGATCCTGATTCGCGCCTTCGAGATGGAGGAGATCAGTGAA
CGGCCTTTCCGAATTGACGGCGATGGATACGTGAACCTTCCCGTCCTGGGCAGGGTGAAGTGCGGTGGGATTACGGTCGA
AAAGTTTGAATCGACGTTGCTGGATGCGCTGCGAAAATACGTCCGGCAACCGCAGGTGACGGTCACGGTGGTGCAGTTCA
GCAGCGAGCCGGTATTTTTCGTGGGAGCATTCAAGGCGCCTGGAATTTATCCCCTGGAGGGCCGCCGGACTATGGTGGAA
ATGATGGCATCCATCGGAGGATTACAGCCCACGGCGAGCCGCCGGATCAAATTGACCCGTCGCAAGGAATTCGGCGAGAT
TCCGCTCGCGAGCGCAGTTCCGACGCCGGACGGGAGCGCTTCCACCTTAGAGATCAACATGGCGAGCCTCCGGGATAACG
TAAACCCTGCCGAGGACCTGGTACTGCAGCCTTTTGACGTGATCAGTGTCGAGCGCGCCGAGATGGTGTACGTAACTGGG
GAAGTGAATAAAGTTGGGGCTTTTGAGTTGCAGGAGCGCGATTCGATTTCGGTGATCCAGGCGCTGACCCTGGCCGGTGG
GTTGAACCCAAATGCGAACACCAAGACAGCACGGATTCTTCGACCGGTGATGAACACGTCCAGGCGCGCTGAGATCCAAC
TCAATCTGCAGAGCATTTTGGAAGGACACGAGAGTGACCAACCGCTGCTCCCGAACGACGTACTCTACATACCCAAAGGA
ACGGGACTGAAGCACAACCTGGGCAAGACCATGCTAGTGGTGCTGCCGGTGGCAGGCACGATCGCCAGCCTTGCTATCGC
GTTTACACGTTAG

Upstream 100 bases:

>100_bases
ACGATGTCACGGCCGATATTGTCGAGCGGCTTCAACAGTTGGAGAATTCGGTGGAACGTCTCAACCACACAATGACGCAA
AACAGACCGGAGCGGCAGGC

Downstream 100 bases:

>100_bases
GATCTCTAGAATGAACGATCATTACGAGCCCGAGTCGAACGGCTCAGGGCCGCATGGACTCCCTGTCGGGCCTGATGTTC
GCATGCTCCCGAGCCCGAGC

Product: polysaccharide export protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKITMKIPSLMLIGVAVHVSALVAQVPPAQPGGQPRQQLPASQQSVNVASAPMERLRPNYVLRPGDQILIRAFEMEEISE
RPFRIDGDGYVNLPVLGRVKCGGITVEKFESTLLDALRKYVRQPQVTVTVVQFSSEPVFFVGAFKAPGIYPLEGRRTMVE
MMASIGGLQPTASRRIKLTRRKEFGEIPLASAVPTPDGSASTLEINMASLRDNVNPAEDLVLQPFDVISVERAEMVYVTG
EVNKVGAFELQERDSISVIQALTLAGGLNPNANTKTARILRPVMNTSRRAEIQLNLQSILEGHESDQPLLPNDVLYIPKG
TGLKHNLGKTMLVVLPVAGTIASLAIAFTR

Sequences:

>Translated_350_residues
MKITMKIPSLMLIGVAVHVSALVAQVPPAQPGGQPRQQLPASQQSVNVASAPMERLRPNYVLRPGDQILIRAFEMEEISE
RPFRIDGDGYVNLPVLGRVKCGGITVEKFESTLLDALRKYVRQPQVTVTVVQFSSEPVFFVGAFKAPGIYPLEGRRTMVE
MMASIGGLQPTASRRIKLTRRKEFGEIPLASAVPTPDGSASTLEINMASLRDNVNPAEDLVLQPFDVISVERAEMVYVTG
EVNKVGAFELQERDSISVIQALTLAGGLNPNANTKTARILRPVMNTSRRAEIQLNLQSILEGHESDQPLLPNDVLYIPKG
TGLKHNLGKTMLVVLPVAGTIASLAIAFTR
>Mature_350_residues
MKITMKIPSLMLIGVAVHVSALVAQVPPAQPGGQPRQQLPASQQSVNVASAPMERLRPNYVLRPGDQILIRAFEMEEISE
RPFRIDGDGYVNLPVLGRVKCGGITVEKFESTLLDALRKYVRQPQVTVTVVQFSSEPVFFVGAFKAPGIYPLEGRRTMVE
MMASIGGLQPTASRRIKLTRRKEFGEIPLASAVPTPDGSASTLEINMASLRDNVNPAEDLVLQPFDVISVERAEMVYVTG
EVNKVGAFELQERDSISVIQALTLAGGLNPNANTKTARILRPVMNTSRRAEIQLNLQSILEGHESDQPLLPNDVLYIPKG
TGLKHNLGKTMLVVLPVAGTIASLAIAFTR

Specific function: Involved in the biosynthesis of amylovoran which functions as a virulence factor. Might be involved in the translocation of polysaccharides across the outer membrane [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1787218, Length=291, Percent_Identity=23.7113402061856, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 38110; Mature: 38110

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITMKIPSLMLIGVAVHVSALVAQVPPAQPGGQPRQQLPASQQSVNVASAPMERLRPNY
CEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHCCCHHHCCHHHCCCCE
VLRPGDQILIRAFEMEEISERPFRIDGDGYVNLPVLGRVKCGGITVEKFESTLLDALRKY
EECCCCEEEEEEECHHHHCCCCEEECCCCEECCCEEEEEEECCEEHHHHHHHHHHHHHHH
VRQPQVTVTVVQFSSEPVFFVGAFKAPGIYPLEGRRTMVEMMASIGGLQPTASRRIKLTR
HCCCCEEEEEEEECCCCEEEEEECCCCCEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEE
RKEFGEIPLASAVPTPDGSASTLEINMASLRDNVNPAEDLVLQPFDVISVERAEMVYVTG
HHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCHHHHHHCCCCCCEEECCEEEEEEEC
EVNKVGAFELQERDSISVIQALTLAGGLNPNANTKTARILRPVMNTSRRAEIQLNLQSIL
CCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHH
EGHESDQPLLPNDVLYIPKGTGLKHNLGKTMLVVLPVAGTIASLAIAFTR
CCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECHHHHHHHHEEECC
>Mature Secondary Structure
MKITMKIPSLMLIGVAVHVSALVAQVPPAQPGGQPRQQLPASQQSVNVASAPMERLRPNY
CEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHCCCHHHCCHHHCCCCE
VLRPGDQILIRAFEMEEISERPFRIDGDGYVNLPVLGRVKCGGITVEKFESTLLDALRKY
EECCCCEEEEEEECHHHHCCCCEEECCCCEECCCEEEEEEECCEEHHHHHHHHHHHHHHH
VRQPQVTVTVVQFSSEPVFFVGAFKAPGIYPLEGRRTMVEMMASIGGLQPTASRRIKLTR
HCCCCEEEEEEEECCCCEEEEEECCCCCEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEE
RKEFGEIPLASAVPTPDGSASTLEINMASLRDNVNPAEDLVLQPFDVISVERAEMVYVTG
HHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCHHHHHHCCCCCCEEECCEEEEEEEC
EVNKVGAFELQERDSISVIQALTLAGGLNPNANTKTARILRPVMNTSRRAEIQLNLQSIL
CCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHH
EGHESDQPLLPNDVLYIPKGTGLKHNLGKTMLVVLPVAGTIASLAIAFTR
CCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]