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The map label for this gene is gmd [H]

Identifier: 116623720

GI number: 116623720

Start: 5860364

End: 5861392

Strand: Reverse

Name: gmd [H]

Synonym: Acid_4632

Alternate gene names: 116623720

Gene position: 5861392-5860364 (Counterclockwise)

Preceding gene: 116623721

Following gene: 116623719

Centisome position: 58.82

GC content: 58.89

Gene sequence:

>1029_bases
ATGAAGAAGGCATTGATCACAGGTATCACCGGCCAGGATGGCTCGTTTTTGGCCGAGTTCCTCCTCGAAAAAGGCTACGA
AGTTCACGGGCTCGTCCGTCGATCGTCAACGTTTACCACCGGGCGTCTCGAGCACCTGTACATCGATCCCCACACTCCTC
ATGCCCGGCTCCTCCTGCACTACGGCGACTTGAGCGACGGAGGAGGAATTCGGCGGCTCCTGGAACAGATTCAGCCGGAC
GAAGTCTACAATCTCGCAGCCCAGTCCCACGTACGCGTCAGCTTCGACCAGGCCGAGTTTACGGCTGATGTGGTCGGTCT
TGGAGCCTTGCGGATTTTGGAAGCCGTCCGAAATGCGGCGGAAGCAGGCAATAACCCCATCCGCTTTTATCAGGCTGGCT
CGTCGGAGATGTTCGGCGCCGCGGCGCCTCCTCAGAACGAAGCCACTCCCTTTTACCCCCGCAGTCCGTACGCTGCCTCC
AAAGTCGCGGCACACTGGTACGCCATCAATTACCGCGAGTCGTATGGCCTCTTCATTGCGAACGGTATTCTCTTTAACCA
TGAATCCGAACGGCGCGGCGAGACTTTCGTAACTCGCAAAATCACCCGTGCTCTGACGCGCATTCACCTCGGCCTGCAAG
ACAAGCTCTACCTCGGAAATCTTGATGCACGCCGCGATTGGGGTCACGCAGCGGACTATGTGCGCGCTATGTGGCTCATG
CTGCAGCAGGACCGGCCCGACGACTACGTGATCGCCACAGGGGAATCTTACTCGGTGCGGGAATTCCTCGAACGTGCGGC
CGCTCATCTTGGAATGGACTGGCAGAAGGTCGTCGAACAGGATCCACGCTACCTGCGCCCCACCGAAGTGGATTTCCTGA
AAGGTGATCCCTCCAAGGCGCGCGAACAACTCGGTTGGAAGCCGGAGGTCACGTTCGATCAATTGGTGAAGCGCATGGTC
GAGCACGATCTGGAGTTGGCGCACCGGGAGCAATTGCTGGCGGCCCGTGTGAGGGTTGGGCATGCATGA

Upstream 100 bases:

>100_bases
TGTACCGATTCGCGTACTGTAACCCCTGTACATTGACAAATTGTTGCAGGGAGTCAATCATAGGTAGCTTCGGCGTAATA
CGATCACGAAAAAGGGTGAC

Downstream 100 bases:

>100_bases
AGAGAGCCGGATCTTTGTAGCTGGACACCGGGGTCTGGCCGGGTCCGCCCTGGTTCGCGCGCTCCAGCGGTCGGGGTATA
GGAATCTGCTCTTGCGCAGC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKKALITGITGQDGSFLAEFLLEKGYEVHGLVRRSSTFTTGRLEHLYIDPHTPHARLLLHYGDLSDGGGIRRLLEQIQPD
EVYNLAAQSHVRVSFDQAEFTADVVGLGALRILEAVRNAAEAGNNPIRFYQAGSSEMFGAAAPPQNEATPFYPRSPYAAS
KVAAHWYAINYRESYGLFIANGILFNHESERRGETFVTRKITRALTRIHLGLQDKLYLGNLDARRDWGHAADYVRAMWLM
LQQDRPDDYVIATGESYSVREFLERAAAHLGMDWQKVVEQDPRYLRPTEVDFLKGDPSKAREQLGWKPEVTFDQLVKRMV
EHDLELAHREQLLAARVRVGHA

Sequences:

>Translated_342_residues
MKKALITGITGQDGSFLAEFLLEKGYEVHGLVRRSSTFTTGRLEHLYIDPHTPHARLLLHYGDLSDGGGIRRLLEQIQPD
EVYNLAAQSHVRVSFDQAEFTADVVGLGALRILEAVRNAAEAGNNPIRFYQAGSSEMFGAAAPPQNEATPFYPRSPYAAS
KVAAHWYAINYRESYGLFIANGILFNHESERRGETFVTRKITRALTRIHLGLQDKLYLGNLDARRDWGHAADYVRAMWLM
LQQDRPDDYVIATGESYSVREFLERAAAHLGMDWQKVVEQDPRYLRPTEVDFLKGDPSKAREQLGWKPEVTFDQLVKRMV
EHDLELAHREQLLAARVRVGHA
>Mature_342_residues
MKKALITGITGQDGSFLAEFLLEKGYEVHGLVRRSSTFTTGRLEHLYIDPHTPHARLLLHYGDLSDGGGIRRLLEQIQPD
EVYNLAAQSHVRVSFDQAEFTADVVGLGALRILEAVRNAAEAGNNPIRFYQAGSSEMFGAAAPPQNEATPFYPRSPYAAS
KVAAHWYAINYRESYGLFIANGILFNHESERRGETFVTRKITRALTRIHLGLQDKLYLGNLDARRDWGHAADYVRAMWLM
LQQDRPDDYVIATGESYSVREFLERAAAHLGMDWQKVVEQDPRYLRPTEVDFLKGDPSKAREQLGWKPEVTFDQLVKRMV
EHDLELAHREQLLAARVRVGHA

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=57.3099415204678, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=24.3243243243243, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788366, Length=362, Percent_Identity=55.524861878453, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=25, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=24.7838616714697, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=54.3604651162791, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI133901790, Length=344, Percent_Identity=53.7790697674419, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17539424, Length=344, Percent_Identity=53.7790697674419, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI133901788, Length=344, Percent_Identity=53.7790697674419, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17539422, Length=344, Percent_Identity=53.7790697674419, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI133901786, Length=344, Percent_Identity=53.7790697674419, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24158427, Length=350, Percent_Identity=54.5714285714286, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38774; Mature: 38774

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALITGITGQDGSFLAEFLLEKGYEVHGLVRRSSTFTTGRLEHLYIDPHTPHARLLLH
CCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCEEEEEE
YGDLSDGGGIRRLLEQIQPDEVYNLAAQSHVRVSFDQAEFTADVVGLGALRILEAVRNAA
ECCCCCCHHHHHHHHHCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
EAGNNPIRFYQAGSSEMFGAAAPPQNEATPFYPRSPYAASKVAAHWYAINYRESYGLFIA
HCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEEECCCCEEEE
NGILFNHESERRGETFVTRKITRALTRIHLGLQDKLYLGNLDARRDWGHAADYVRAMWLM
CCEEECCCCHHCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
LQQDRPDDYVIATGESYSVREFLERAAAHLGMDWQKVVEQDPRYLRPTEVDFLKGDPSKA
HHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEECCCHHHH
REQLGWKPEVTFDQLVKRMVEHDLELAHREQLLAARVRVGHA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKALITGITGQDGSFLAEFLLEKGYEVHGLVRRSSTFTTGRLEHLYIDPHTPHARLLLH
CCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCEEEEEE
YGDLSDGGGIRRLLEQIQPDEVYNLAAQSHVRVSFDQAEFTADVVGLGALRILEAVRNAA
ECCCCCCHHHHHHHHHCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
EAGNNPIRFYQAGSSEMFGAAAPPQNEATPFYPRSPYAASKVAAHWYAINYRESYGLFIA
HCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEEECCCCEEEE
NGILFNHESERRGETFVTRKITRALTRIHLGLQDKLYLGNLDARRDWGHAADYVRAMWLM
CCEEECCCCHHCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
LQQDRPDDYVIATGESYSVREFLERAAAHLGMDWQKVVEQDPRYLRPTEVDFLKGDPSKA
HHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEECCCHHHH
REQLGWKPEVTFDQLVKRMVEHDLELAHREQLLAARVRVGHA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]