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The map label for this gene is fcl [H]

Identifier: 116623719

GI number: 116623719

Start: 5859415

End: 5860371

Strand: Reverse

Name: fcl [H]

Synonym: Acid_4631

Alternate gene names: 116623719

Gene position: 5860371-5859415 (Counterclockwise)

Preceding gene: 116623720

Following gene: 116623717

Centisome position: 58.81

GC content: 59.35

Gene sequence:

>957_bases
ATGCATGAAGAGAGCCGGATCTTTGTAGCTGGACACCGGGGTCTGGCCGGGTCCGCCCTGGTTCGCGCGCTCCAGCGGTC
GGGGTATAGGAATCTGCTCTTGCGCAGCCGCGAGGAATTGGACCTTATGGACCAGCCCGCGGTCTTTCGCTTCTTCGAAA
TCGAAAGACCCGAATACGTCTTCCTGGCCGCTGCCAAGGTCGGCGGCATTCTTGCGAACTCCACGTTCCCCGTTGATTTC
CTGCGGGACAATCTTGCGGTTCAAGCGAACGTCACGGAAGCCGCTTTTCGATTCCGCGTCGAGAAGCTCCTGTTTCTCGG
ATCTTCCTGCATTTATCCGAGAATGGCGGCGCAGCCGATCTCGGAATCCGCTCTGCTAACCGGGCCCCTGGAGCCCACCA
ATGAATGGTACGCGATCGCCAAAATCGCGGGCATCAAGCTGATTCAGGCCTATCGAAAACAGTATGGCATGAAAGGAATC
TGCCTAATGCCGACGAACCTCTACGGACCAGGTGACAATTTCGATCTCGCCAGCTCCCACGTCTTGCCGGCCTTGCTCCG
AAAATTTCACGAGGCCAAGCTGAACGGCGCGGACGAAGTGGTCGTATGGGGTAGCGGCAAGGTGCTTCGCGAATTCCTGC
ACGCGGACGATTTTGCGGACGCGGCGGTGCACTTGATGCTTCACTATGACAGCGCCGAGATCATCAACGTCGGAACCGGG
GAAGACCTCACGATCGCGGCCTTGGCGGAGTTGATCGGAAAAGTCGTCGGCTACCCCGGCCGCATTACCTTCGACGCCAC
CAAGCCTGATGGTACGCCCCGCAAACTCCTCGACGTTACGCGGTTGCGTGCCGCCGGATGGCGAGCGCGCATCACCCTCG
AAGAGGGACTCCAAAGCACCTACGAGTGGTACCTGGAGCAGTCTGCTGGAAAGCTATCGGCCGTCCTGCGGCAGTAG

Upstream 100 bases:

>100_bases
CCGGAGGTCACGTTCGATCAATTGGTGAAGCGCATGGTCGAGCACGATCTGGAGTTGGCGCACCGGGAGCAATTGCTGGC
GGCCCGTGTGAGGGTTGGGC

Downstream 100 bases:

>100_bases
CGGGCGCCTTCAGCCCTTTCCCGCCTTCAAAGCGCCACGCAAGCACACATCCCAACGATCCAACGCCTGCCCACGCGAGA
ACTTCTCCATATACACCTTG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MHEESRIFVAGHRGLAGSALVRALQRSGYRNLLLRSREELDLMDQPAVFRFFEIERPEYVFLAAAKVGGILANSTFPVDF
LRDNLAVQANVTEAAFRFRVEKLLFLGSSCIYPRMAAQPISESALLTGPLEPTNEWYAIAKIAGIKLIQAYRKQYGMKGI
CLMPTNLYGPGDNFDLASSHVLPALLRKFHEAKLNGADEVVVWGSGKVLREFLHADDFADAAVHLMLHYDSAEIINVGTG
EDLTIAALAELIGKVVGYPGRITFDATKPDGTPRKLLDVTRLRAAGWRARITLEEGLQSTYEWYLEQSAGKLSAVLRQ

Sequences:

>Translated_318_residues
MHEESRIFVAGHRGLAGSALVRALQRSGYRNLLLRSREELDLMDQPAVFRFFEIERPEYVFLAAAKVGGILANSTFPVDF
LRDNLAVQANVTEAAFRFRVEKLLFLGSSCIYPRMAAQPISESALLTGPLEPTNEWYAIAKIAGIKLIQAYRKQYGMKGI
CLMPTNLYGPGDNFDLASSHVLPALLRKFHEAKLNGADEVVVWGSGKVLREFLHADDFADAAVHLMLHYDSAEIINVGTG
EDLTIAALAELIGKVVGYPGRITFDATKPDGTPRKLLDVTRLRAAGWRARITLEEGLQSTYEWYLEQSAGKLSAVLRQ
>Mature_318_residues
MHEESRIFVAGHRGLAGSALVRALQRSGYRNLLLRSREELDLMDQPAVFRFFEIERPEYVFLAAAKVGGILANSTFPVDF
LRDNLAVQANVTEAAFRFRVEKLLFLGSSCIYPRMAAQPISESALLTGPLEPTNEWYAIAKIAGIKLIQAYRKQYGMKGI
CLMPTNLYGPGDNFDLASSHVLPALLRKFHEAKLNGADEVVVWGSGKVLREFLHADDFADAAVHLMLHYDSAEIINVGTG
EDLTIAALAELIGKVVGYPGRITFDATKPDGTPRKLLDVTRLRAAGWRARITLEEGLQSTYEWYLEQSAGKLSAVLRQ

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=308, Percent_Identity=31.8181818181818, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=23.1927710843373, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788365, Length=313, Percent_Identity=57.8274760383387, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1788353, Length=351, Percent_Identity=24.7863247863248, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=22.1264367816092, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=307, Percent_Identity=29.3159609120521, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17568069, Length=281, Percent_Identity=24.5551601423488, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922778, Length=313, Percent_Identity=29.3929712460064, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21356223, Length=324, Percent_Identity=27.4691358024691, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 35255; Mature: 35255

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEESRIFVAGHRGLAGSALVRALQRSGYRNLLLRSREELDLMDQPAVFRFFEIERPEYV
CCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHCCHHHCCCCCCCCEEEEEECCCCCEE
FLAAAKVGGILANSTFPVDFLRDNLAVQANVTEAAFRFRVEKLLFLGSSCIYPRMAAQPI
EEEEHHHCCEEECCCCCHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
SESALLTGPLEPTNEWYAIAKIAGIKLIQAYRKQYGMKGICLMPTNLYGPGDNFDLASSH
CCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHH
VLPALLRKFHEAKLNGADEVVVWGSGKVLREFLHADDFADAAVHLMLHYDSAEIINVGTG
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCHHHHEEEEEEEECCCEEEECCCC
EDLTIAALAELIGKVVGYPGRITFDATKPDGTPRKLLDVTRLRAAGWRARITLEEGLQST
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHH
YEWYLEQSAGKLSAVLRQ
HHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MHEESRIFVAGHRGLAGSALVRALQRSGYRNLLLRSREELDLMDQPAVFRFFEIERPEYV
CCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHCCHHHCCCCCCCCEEEEEECCCCCEE
FLAAAKVGGILANSTFPVDFLRDNLAVQANVTEAAFRFRVEKLLFLGSSCIYPRMAAQPI
EEEEHHHCCEEECCCCCHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
SESALLTGPLEPTNEWYAIAKIAGIKLIQAYRKQYGMKGICLMPTNLYGPGDNFDLASSH
CCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHH
VLPALLRKFHEAKLNGADEVVVWGSGKVLREFLHADDFADAAVHLMLHYDSAEIINVGTG
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCHHHHEEEEEEEECCCEEEECCCC
EDLTIAALAELIGKVVGYPGRITFDATKPDGTPRKLLDVTRLRAAGWRARITLEEGLQST
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHH
YEWYLEQSAGKLSAVLRQ
HHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]