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The map label for this gene is cca [H]

Identifier: 116623706

GI number: 116623706

Start: 5836953

End: 5838221

Strand: Reverse

Name: cca [H]

Synonym: Acid_4618

Alternate gene names: 116623706

Gene position: 5838221-5836953 (Counterclockwise)

Preceding gene: 116623710

Following gene: 116623705

Centisome position: 58.58

GC content: 63.75

Gene sequence:

>1269_bases
TTGCGCGGCGCGGGACATCAGGCATATCTGGTCGGCGGCTGCGTCCGCGATCACCTCCTCGGCTGCAAGCCGAAGGACTA
CGATATCGCCACGGACGCGCGCCCCGACCGCATCATGGACCTCTTCCCGAGTTCCGGCCGGGTCGGCGCCCACTTCGGCG
TAATCCTGGTGCGCGACGTCTTCGATCAGGTCGAAGTCGCCACCTTCCGCAGCGATCACGATTACCTGGATGGGCGGCAT
CCGGAGCAGGTGCGCTTCGAGTGCGATCCTCGCGAAGACGTTCTGCGCCGCGACTTCACCATCAACGGCCTGATGCTCGA
TCCCGACACTGGCCGCGTGCTCGATTTTGTGGATGGGCAGGGCGACCTCAAACGCGGCATCGTGCGTGCCATAGGCGACC
CTTACGCCCGGTTCCGCGAAGATCACCTGCGGTTGCTGCGCGCCGTGCGCTTCGCGGCTCGCCTGGGCTTTGAAATCGAG
CCCGCCACCTTCGACGCCATCCGCCGCGACCACGCCTTGATCCTCAAGGTCGCTATGGAGCGGATCCGTGAGGAACTGGT
GCGGATCCTCACCGAAGGCGGCGCGCGCCGCGGCTTCGAACTGCTCGACGCCGCCGGCATGCTCGCCGACCTTTTGCCCG
AGGTGGCGGCCATGAAAGGCGTGGACCAGCCTCCGCAATTTCATCCCGAGGGCGATGTGTGGGCCCACACGCTCCTCCTC
CTGGAGCAGTTGCGCAATCCCACGCCGACGCTCGCCCTCGGAGCGCTGCTGCACGATGTCGGTAAGCCGCCGACCTTCCG
CGTGGCGGACCGCATCCGCTTCGACGGCCATGTCGAAGAAGGTGTGCGAATCGCCCATGCCATTCTCACGCGCCTGCGTT
TTTCCCGGGAGGAGATCGACCAGGTCGAAGCGCTCGTCGAAAATCACATGCGCTTTAAAGACGTGCACCACATGAAGGAG
AGCACGTTGAAGCGCTTCTTGCGCCTGCCCGATTTCGAAGAGCATCTCGAACTCCACCGCCTCGACGTGATGAGCAGCAA
CCGCCGGCTGGAGAATTACGAACTGGCCAGGCGCAAGCTCGCCGAGTACAGCGAAAAACACCTGCAGCCGGAGCCTTTGG
TAACCGGCGCCGATTTGATCGGGATGGGCTACCAGCCCGGACCGCAATTCTCGCGGATCCTTTCGGTCATTGAAGACGCC
CAGCTCGAAGGCACCATTCAGACGCGGGGCGAAGCGATTCATCTGATCCGCGACACCTTTCCCCTTTAA

Upstream 100 bases:

>100_bases
AAAAAAACTTGGGTCCCCCGTCTCCAGGTGGAGCTCGGCGACTTCATATTAGCATAGGAAGGTGGAATCGGAAGCGCTTG
CACGCCGGGTAATCGCCAAG

Downstream 100 bases:

>100_bases
CTACGTCTGTTAGTCCGACAGGGGGACAAGCATCATGTTACGTAGTTGTCTTTCGATCGGTATCCTGCTGGCCGCCGGCT
CCGCCGCGCTGCCCGCGCAA

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MRGAGHQAYLVGGCVRDHLLGCKPKDYDIATDARPDRIMDLFPSSGRVGAHFGVILVRDVFDQVEVATFRSDHDYLDGRH
PEQVRFECDPREDVLRRDFTINGLMLDPDTGRVLDFVDGQGDLKRGIVRAIGDPYARFREDHLRLLRAVRFAARLGFEIE
PATFDAIRRDHALILKVAMERIREELVRILTEGGARRGFELLDAAGMLADLLPEVAAMKGVDQPPQFHPEGDVWAHTLLL
LEQLRNPTPTLALGALLHDVGKPPTFRVADRIRFDGHVEEGVRIAHAILTRLRFSREEIDQVEALVENHMRFKDVHHMKE
STLKRFLRLPDFEEHLELHRLDVMSSNRRLENYELARRKLAEYSEKHLQPEPLVTGADLIGMGYQPGPQFSRILSVIEDA
QLEGTIQTRGEAIHLIRDTFPL

Sequences:

>Translated_422_residues
MRGAGHQAYLVGGCVRDHLLGCKPKDYDIATDARPDRIMDLFPSSGRVGAHFGVILVRDVFDQVEVATFRSDHDYLDGRH
PEQVRFECDPREDVLRRDFTINGLMLDPDTGRVLDFVDGQGDLKRGIVRAIGDPYARFREDHLRLLRAVRFAARLGFEIE
PATFDAIRRDHALILKVAMERIREELVRILTEGGARRGFELLDAAGMLADLLPEVAAMKGVDQPPQFHPEGDVWAHTLLL
LEQLRNPTPTLALGALLHDVGKPPTFRVADRIRFDGHVEEGVRIAHAILTRLRFSREEIDQVEALVENHMRFKDVHHMKE
STLKRFLRLPDFEEHLELHRLDVMSSNRRLENYELARRKLAEYSEKHLQPEPLVTGADLIGMGYQPGPQFSRILSVIEDA
QLEGTIQTRGEAIHLIRDTFPL
>Mature_422_residues
MRGAGHQAYLVGGCVRDHLLGCKPKDYDIATDARPDRIMDLFPSSGRVGAHFGVILVRDVFDQVEVATFRSDHDYLDGRH
PEQVRFECDPREDVLRRDFTINGLMLDPDTGRVLDFVDGQGDLKRGIVRAIGDPYARFREDHLRLLRAVRFAARLGFEIE
PATFDAIRRDHALILKVAMERIREELVRILTEGGARRGFELLDAAGMLADLLPEVAAMKGVDQPPQFHPEGDVWAHTLLL
LEQLRNPTPTLALGALLHDVGKPPTFRVADRIRFDGHVEEGVRIAHAILTRLRFSREEIDQVEALVENHMRFKDVHHMKE
STLKRFLRLPDFEEHLELHRLDVMSSNRRLENYELARRKLAEYSEKHLQPEPLVTGADLIGMGYQPGPQFSRILSVIEDA
QLEGTIQTRGEAIHLIRDTFPL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=230, Percent_Identity=35.6521739130435, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI87081691, Length=226, Percent_Identity=38.9380530973451, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789436, Length=318, Percent_Identity=34.2767295597484, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71995920, Length=194, Percent_Identity=34.020618556701, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321016, Length=210, Percent_Identity=30.952380952381, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281360112, Length=194, Percent_Identity=36.5979381443299, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357337, Length=194, Percent_Identity=36.5979381443299, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 48103; Mature: 48103

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGAGHQAYLVGGCVRDHLLGCKPKDYDIATDARPDRIMDLFPSSGRVGAHFGVILVRDV
CCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
FDQVEVATFRSDHDYLDGRHPEQVRFECDPREDVLRRDFTINGLMLDPDTGRVLDFVDGQ
HHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHCCCEECCEEECCCCCCEEEECCCC
GDLKRGIVRAIGDPYARFREDHLRLLRAVRFAARLGFEIEPATFDAIRRDHALILKVAME
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
RIREELVRILTEGGARRGFELLDAAGMLADLLPEVAAMKGVDQPPQFHPEGDVWAHTLLL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
LEQLRNPTPTLALGALLHDVGKPPTFRVADRIRFDGHVEEGVRIAHAILTRLRFSREEID
HHHHCCCCCHHHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHH
QVEALVENHMRFKDVHHMKESTLKRFLRLPDFEEHLELHRLDVMSSNRRLENYELARRKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AEYSEKHLQPEPLVTGADLIGMGYQPGPQFSRILSVIEDAQLEGTIQTRGEAIHLIRDTF
HHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCEEEHHHHCC
PL
CC
>Mature Secondary Structure
MRGAGHQAYLVGGCVRDHLLGCKPKDYDIATDARPDRIMDLFPSSGRVGAHFGVILVRDV
CCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
FDQVEVATFRSDHDYLDGRHPEQVRFECDPREDVLRRDFTINGLMLDPDTGRVLDFVDGQ
HHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHCCCEECCEEECCCCCCEEEECCCC
GDLKRGIVRAIGDPYARFREDHLRLLRAVRFAARLGFEIEPATFDAIRRDHALILKVAME
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
RIREELVRILTEGGARRGFELLDAAGMLADLLPEVAAMKGVDQPPQFHPEGDVWAHTLLL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
LEQLRNPTPTLALGALLHDVGKPPTFRVADRIRFDGHVEEGVRIAHAILTRLRFSREEID
HHHHCCCCCHHHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHH
QVEALVENHMRFKDVHHMKESTLKRFLRLPDFEEHLELHRLDVMSSNRRLENYELARRKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AEYSEKHLQPEPLVTGADLIGMGYQPGPQFSRILSVIEDAQLEGTIQTRGEAIHLIRDTF
HHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCEEEHHHHCC
PL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA