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The map label for this gene is yfeJ [H]

Identifier: 116623673

GI number: 116623673

Start: 5802125

End: 5802841

Strand: Reverse

Name: yfeJ [H]

Synonym: Acid_4585

Alternate gene names: 116623673

Gene position: 5802841-5802125 (Counterclockwise)

Preceding gene: 116623674

Following gene: 116623672

Centisome position: 58.23

GC content: 62.34

Gene sequence:

>717_bases
ATGCGCGTGCTCGCGTTTCGCCACGTTCCTTTCGAAGGCCTCGGCCTGCTGGAGGCGGCGTTGCGCGAGCGGTGCGTAGC
GATAGACTACGTGGATGCCTACCAAGCGAAGGCCATAATCCCGGCCGCCGAAGCGTATACGGCGCTGGTATTTCTCGGCG
GACCGATGTCGGTCAACGACGATCTGGACTATCTCCGGCGCGAGATGGAGATCATCCGCCAGGCCGCGGCGCGCCGGCAA
CCGGTGCTCGGCATCTGCCTGGGGGCGCAGCTGATTGCGAAGGCGATGGGCGCCCGCGTGCAGCCGAACCCAAAGAAGGA
AATCGGGTGGTTTGAAGTGGAGTTTACCGACGCGGCCGCGCGCGACGCTCTGTTCGCGGGATTCTCCAAAGAGACCGTGT
TCCACTGGCATGGAGAGACCTTCGATCTGCCGGAAGGCGCGGAGCTGCTGGCGTCCTCGCGTGACTGCAAGCACCAGGCG
TACCGTCTCGGATCACATCTGTACGGATTCCAGTTTCATCTAGAGGTTACTCCCTCGATGGTGGCGGACTGGTACTCCCA
GGACCAGAACTGCGGTGACGTGCGCGAGTTGACAGATCCCATTGACCCGATGGCAAATTCCTCGCGCATGGCCGAACTCG
CGAGAGTGGTGTTCGGCCGCTGGTGCGATTCCTTGCGGCCGGAAGTGACTACGCAGGCGCAACTTGCCATACTATGA

Upstream 100 bases:

>100_bases
TCGGATTACCGCCAGAAGGTCGCCGACGCGCTTTATCGCGGCGTGGCCCGCTACGCCGAAGGTCTCAGCCATTTTCAAAT
GGCGCAGAATTAATTTTCGC

Downstream 100 bases:

>100_bases
AGTATCGAAAAGTGATATGGCCAAAGATCCGGAACGCAATTCCGAATACGAAGACCAGATCGACGCCGAAGAGTTGGACG
GGTTCGAGGCGGAGGAAGAA

Product: glutamine amidotransferase class-I

Products: AMP; L-Glutamate; GMP; H+; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRVLAFRHVPFEGLGLLEAALRERCVAIDYVDAYQAKAIIPAAEAYTALVFLGGPMSVNDDLDYLRREMEIIRQAAARRQ
PVLGICLGAQLIAKAMGARVQPNPKKEIGWFEVEFTDAAARDALFAGFSKETVFHWHGETFDLPEGAELLASSRDCKHQA
YRLGSHLYGFQFHLEVTPSMVADWYSQDQNCGDVRELTDPIDPMANSSRMAELARVVFGRWCDSLRPEVTTQAQLAIL

Sequences:

>Translated_238_residues
MRVLAFRHVPFEGLGLLEAALRERCVAIDYVDAYQAKAIIPAAEAYTALVFLGGPMSVNDDLDYLRREMEIIRQAAARRQ
PVLGICLGAQLIAKAMGARVQPNPKKEIGWFEVEFTDAAARDALFAGFSKETVFHWHGETFDLPEGAELLASSRDCKHQA
YRLGSHLYGFQFHLEVTPSMVADWYSQDQNCGDVRELTDPIDPMANSSRMAELARVVFGRWCDSLRPEVTTQAQLAIL
>Mature_238_residues
MRVLAFRHVPFEGLGLLEAALRERCVAIDYVDAYQAKAIIPAAEAYTALVFLGGPMSVNDDLDYLRREMEIIRQAAARRQ
PVLGICLGAQLIAKAMGARVQPNPKKEIGWFEVEFTDAAARDALFAGFSKETVFHWHGETFDLPEGAELLASSRDCKHQA
YRLGSHLYGFQFHLEVTPSMVADWYSQDQNCGDVRELTDPIDPMANSSRMAELARVVFGRWCDSLRPEVTTQAQLAIL

Specific function: Catalyzes The Synthesis Of Gmp From Xmp. [C]

COG id: COG0518

COG function: function code F; GMP synthase - Glutamine amidotransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 6.3.5.2 [C]

Molecular weight: Translated: 26608; Mature: 26608

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLAFRHVPFEGLGLLEAALRERCVAIDYVDAYQAKAIIPAAEAYTALVFLGGPMSVND
CCEEEEECCCCCCHHHHHHHHHHHHEEEEHHHHHHHHHHCCHHHHHEEEEEECCCCCCCC
DLDYLRREMEIIRQAAARRQPVLGICLGAQLIAKAMGARVQPNPKKEIGWFEVEFTDAAA
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHH
RDALFAGFSKETVFHWHGETFDLPEGAELLASSRDCKHQAYRLGSHLYGFQFHLEVTPSM
HHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHCHHEEEEEEEEECHHH
VADWYSQDQNCGDVRELTDPIDPMANSSRMAELARVVFGRWCDSLRPEVTTQAQLAIL
HHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
>Mature Secondary Structure
MRVLAFRHVPFEGLGLLEAALRERCVAIDYVDAYQAKAIIPAAEAYTALVFLGGPMSVND
CCEEEEECCCCCCHHHHHHHHHHHHEEEEHHHHHHHHHHCCHHHHHEEEEEECCCCCCCC
DLDYLRREMEIIRQAAARRQPVLGICLGAQLIAKAMGARVQPNPKKEIGWFEVEFTDAAA
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHH
RDALFAGFSKETVFHWHGETFDLPEGAELLASSRDCKHQAYRLGSHLYGFQFHLEVTPSM
HHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHCHHEEEEEEEEECHHH
VADWYSQDQNCGDVRELTDPIDPMANSSRMAELARVVFGRWCDSLRPEVTTQAQLAIL
HHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1 {NH4+}} 0.42 {6-thioXMP}} 0.53 {ATP}} 2 {1-ribosyl-4,6-dihydroxypyrazolo[3,4-d]-pyrimidine} 0.058 {XMP}} 0.029 {XMP}} 0.35 {8-azaXMP}} [C]

Substrates: ATP; L-Glutamine; H2O; Xanthosine 5'-phosphate [C]

Specific reaction: ATP + L-Glutamine + H2O + Xanthosine 5'-phosphate --> AMP + L-Glutamate + GMP + (2) H+ + Diphosphate [C]

General reaction: Amination [C]

Inhibitor: 1, N6-Ethenoadenosine; 1-Methyl adenosine 5'-phosphate 5'-phosphate; 2', 3'-Dihydroadenosine; 2'-Deoxyadenosine; 2, 6-Diaminopurine ribonucleoside; 2-Amino-6-methyl thiopurine ribonucleoside; 2-Fluoroadenosine; 3'-Deoxyadenosine; 5'-Azido-5'-deoxyadenosin

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7612925; 11677609 [H]