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The map label for this gene is pbpE [H]

Identifier: 116623668

GI number: 116623668

Start: 5795466

End: 5796956

Strand: Direct

Name: pbpE [H]

Synonym: Acid_4580

Alternate gene names: 116623668

Gene position: 5795466-5796956 (Clockwise)

Preceding gene: 116623666

Following gene: 116623669

Centisome position: 58.15

GC content: 65.93

Gene sequence:

>1491_bases
ATGACTCGACGCACCCTGGCACTGCTCTGCCTTCTCTCCGCGGCCGCCGCCGGCCAGAAGCCCCCCGTCGACACGCGCGC
CCGCATCGATCGCGTCTTCGCGCAATTCAACCGCACCGATAGTCCGGGCTGCGCCGTGGGCGTGTCGCTGGATGGCGCCA
CTGTCGCGAGTCTGGCCTATGGCATGGCGGACCTGGAGCACGATGTGCCCCTGCGGCCGGATTCCATCTTCGAACCCGGC
TCCGTCACCAAACAGTTCACCGCCGCCGCCGTTCTGCTGCTCGCTCAGCAGGGCAAACTCTCGCTCGACGATCCCGCGCG
CAAGTATCTTCCGGAGCTGCCGGATTATGGCGCGCCGCTTACCATTCGTCACCTGCTCAACCACACCAGCGGACTGCGCG
ACTGGGGAAGCGTGGAAGCCATCGCCGGCTGGCCGCGCACCACGCGCGCCTACACCCACGCGCACGTGCTCGACATCCTG
AGCCGGCAGCGCTCGCTGAATTACACGCCGGGCGCCGAGTATTCATACACCAATAGCGGCTACAACCTGGCGGCCATCCT
GGTGGGCCGCGTCTCCGGCAAGCCGCTCGCCGAATTCACCCGCGACGCGCTGTTCACGCCGCTCGGCATGACCTCCACCA
GTTGGCGCGATGATTTCCGCCGCATTGTCAAAGACCGCGCTATCGCTTATTCGCAGAATGGCGGCGTCGTCCGGCAGCTG
ATGCCCTTCGAGGACGTCCACGGCAACGGCGGTCTTCTCACTACCGTGGGCGACCTGCTCCGCTGGAATCAGAAGTTCAC
CATCGCGCACCAGTTGGACCAGGGCAAGCTCAACGACGGGCGCACCATCGCCTACGCCGCGGGACTGATGGTGCTGCACT
ACAAAGGGCTCAACGAAATCAGCCACAGCGGCACCACCGCGGGCTACAACGCGTGGCTGGGCCGCTATCCCGACCAGAAT
CTCTCGGTCGCCGTGCTCTGCAATTCGAGCGCAGCCAACGGCACGCAACTGGGCCATGCGGTGGCCGATATCTTCCTCGG
CGATGCCGACAAGGCTGAACCGCCGCGCACGATGGCGCCCGCGAAGTCCGGATTGTATCGCAGCCTGCGCGACCACTCAA
CCATCGAGGCAGGCGCCGGTTCACGCAATGAATTCGTGGGCGACCGCATGCGCGTTCCGAGCGAGATGGATGGCGGCACG
TGGTACGAGCGCGTGGAGCCGTGGACGCCCTCACCCGGCGAACTGGCCGCGCTCACCGGCGAGTATACCAGCGATGAAGC
CGAGGTCACCTTTCGCGTCGCGGTGGAGCAGGGCCGCCTGGTGATCCATCGCAGGCCGGATGCCGTGATCGCGCTCACGC
CGGCGTACAAGGACGCGTTCCGTTCGTCGCTCGGCACCGTCCGCTTTTTGCGCAATGCCGCGGGCGGCGTCAATGAGCTC
AGCGTGAGCGAGTCACGCGTCTGGGATCTCCGCTTTCGCAAACTGCGGTAA

Upstream 100 bases:

>100_bases
GCAAGAGAACCGAGCCTGACTTTCATAGGGTCACCTGTGGTTTCGGACATCATATCACCGGCTTCATTGGAGTGGGTGGA
AGTAATGTACGCTGGTCCAA

Downstream 100 bases:

>100_bases
TGGATGCGGAGCAGATTCACGGCAGATGCCGGTTTCGATAACATCGTCTTGCACAGGCCTGTAACTTCCGATCTATTCGC
GTAGCGCCGCGTCAAAACTT

Product: beta-lactamase

Products: NA

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTRRTLALLCLLSAAAAGQKPPVDTRARIDRVFAQFNRTDSPGCAVGVSLDGATVASLAYGMADLEHDVPLRPDSIFEPG
SVTKQFTAAAVLLLAQQGKLSLDDPARKYLPELPDYGAPLTIRHLLNHTSGLRDWGSVEAIAGWPRTTRAYTHAHVLDIL
SRQRSLNYTPGAEYSYTNSGYNLAAILVGRVSGKPLAEFTRDALFTPLGMTSTSWRDDFRRIVKDRAIAYSQNGGVVRQL
MPFEDVHGNGGLLTTVGDLLRWNQKFTIAHQLDQGKLNDGRTIAYAAGLMVLHYKGLNEISHSGTTAGYNAWLGRYPDQN
LSVAVLCNSSAANGTQLGHAVADIFLGDADKAEPPRTMAPAKSGLYRSLRDHSTIEAGAGSRNEFVGDRMRVPSEMDGGT
WYERVEPWTPSPGELAALTGEYTSDEAEVTFRVAVEQGRLVIHRRPDAVIALTPAYKDAFRSSLGTVRFLRNAAGGVNEL
SVSESRVWDLRFRKLR

Sequences:

>Translated_496_residues
MTRRTLALLCLLSAAAAGQKPPVDTRARIDRVFAQFNRTDSPGCAVGVSLDGATVASLAYGMADLEHDVPLRPDSIFEPG
SVTKQFTAAAVLLLAQQGKLSLDDPARKYLPELPDYGAPLTIRHLLNHTSGLRDWGSVEAIAGWPRTTRAYTHAHVLDIL
SRQRSLNYTPGAEYSYTNSGYNLAAILVGRVSGKPLAEFTRDALFTPLGMTSTSWRDDFRRIVKDRAIAYSQNGGVVRQL
MPFEDVHGNGGLLTTVGDLLRWNQKFTIAHQLDQGKLNDGRTIAYAAGLMVLHYKGLNEISHSGTTAGYNAWLGRYPDQN
LSVAVLCNSSAANGTQLGHAVADIFLGDADKAEPPRTMAPAKSGLYRSLRDHSTIEAGAGSRNEFVGDRMRVPSEMDGGT
WYERVEPWTPSPGELAALTGEYTSDEAEVTFRVAVEQGRLVIHRRPDAVIALTPAYKDAFRSSLGTVRFLRNAAGGVNEL
SVSESRVWDLRFRKLR
>Mature_495_residues
TRRTLALLCLLSAAAAGQKPPVDTRARIDRVFAQFNRTDSPGCAVGVSLDGATVASLAYGMADLEHDVPLRPDSIFEPGS
VTKQFTAAAVLLLAQQGKLSLDDPARKYLPELPDYGAPLTIRHLLNHTSGLRDWGSVEAIAGWPRTTRAYTHAHVLDILS
RQRSLNYTPGAEYSYTNSGYNLAAILVGRVSGKPLAEFTRDALFTPLGMTSTSWRDDFRRIVKDRAIAYSQNGGVVRQLM
PFEDVHGNGGLLTTVGDLLRWNQKFTIAHQLDQGKLNDGRTIAYAAGLMVLHYKGLNEISHSGTTAGYNAWLGRYPDQNL
SVAVLCNSSAANGTQLGHAVADIFLGDADKAEPPRTMAPAKSGLYRSLRDHSTIEAGAGSRNEFVGDRMRVPSEMDGGTW
YERVEPWTPSPGELAALTGEYTSDEAEVTFRVAVEQGRLVIHRRPDAVIALTPAYKDAFRSSLGTVRFLRNAAGGVNELS
VSESRVWDLRFRKLR

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI26051231, Length=100, Percent_Identity=39, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI26051233, Length=100, Percent_Identity=39, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1786574, Length=230, Percent_Identity=28.695652173913, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082101, Length=278, Percent_Identity=24.8201438848921, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115533026, Length=354, Percent_Identity=27.1186440677966, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17550934, Length=335, Percent_Identity=24.7761194029851, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: NA

Molecular weight: Translated: 54130; Mature: 53998

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRTLALLCLLSAAAAGQKPPVDTRARIDRVFAQFNRTDSPGCAVGVSLDGATVASLAY
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHH
GMADLEHDVPLRPDSIFEPGSVTKQFTAAAVLLLAQQGKLSLDDPARKYLPELPDYGAPL
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCH
TIRHLLNHTSGLRDWGSVEAIAGWPRTTRAYTHAHVLDILSRQRSLNYTPGAEYSYTNSG
HHHHHHHHCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCC
YNLAAILVGRVSGKPLAEFTRDALFTPLGMTSTSWRDDFRRIVKDRAIAYSQNGGVVRQL
CEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEEC
MPFEDVHGNGGLLTTVGDLLRWNQKFTIAHQLDQGKLNDGRTIAYAAGLMVLHYKGLNEI
CCHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEHHHCEEEEEECCCHHH
SHSGTTAGYNAWLGRYPDQNLSVAVLCNSSAANGTQLGHAVADIFLGDADKAEPPRTMAP
HCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
AKSGLYRSLRDHSTIEAGAGSRNEFVGDRMRVPSEMDGGTWYERVEPWTPSPGELAALTG
HHHHHHHHHHHCCCEECCCCCCCHHCCHHHCCCCCCCCCCHHHHCCCCCCCCCCEEEEEC
EYTSDEAEVTFRVAVEQGRLVIHRRPDAVIALTPAYKDAFRSSLGTVRFLRNAAGGVNEL
CCCCCCCEEEEEEEEECCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEE
SVSESRVWDLRFRKLR
CCCCHHEEEHHHHCCC
>Mature Secondary Structure 
TRRTLALLCLLSAAAAGQKPPVDTRARIDRVFAQFNRTDSPGCAVGVSLDGATVASLAY
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHH
GMADLEHDVPLRPDSIFEPGSVTKQFTAAAVLLLAQQGKLSLDDPARKYLPELPDYGAPL
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCH
TIRHLLNHTSGLRDWGSVEAIAGWPRTTRAYTHAHVLDILSRQRSLNYTPGAEYSYTNSG
HHHHHHHHCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCC
YNLAAILVGRVSGKPLAEFTRDALFTPLGMTSTSWRDDFRRIVKDRAIAYSQNGGVVRQL
CEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEEC
MPFEDVHGNGGLLTTVGDLLRWNQKFTIAHQLDQGKLNDGRTIAYAAGLMVLHYKGLNEI
CCHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEHHHCEEEEEECCCHHH
SHSGTTAGYNAWLGRYPDQNLSVAVLCNSSAANGTQLGHAVADIFLGDADKAEPPRTMAP
HCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
AKSGLYRSLRDHSTIEAGAGSRNEFVGDRMRVPSEMDGGTWYERVEPWTPSPGELAALTG
HHHHHHHHHHHCCCEECCCCCCCHHCCHHHCCCCCCCCCCHHHHCCCCCCCCCCEEEEEC
EYTSDEAEVTFRVAVEQGRLVIHRRPDAVIALTPAYKDAFRSSLGTVRFLRNAAGGVNEL
CCCCCCCEEEEEEEEECCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEE
SVSESRVWDLRFRKLR
CCCCHHEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]