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The map label for this gene is pspA [C]

Identifier: 116623658

GI number: 116623658

Start: 5784436

End: 5785095

Strand: Reverse

Name: pspA [C]

Synonym: Acid_4570

Alternate gene names: 116623658

Gene position: 5785095-5784436 (Counterclockwise)

Preceding gene: 116623659

Following gene: 116623657

Centisome position: 58.05

GC content: 59.09

Gene sequence:

>660_bases
ATGGCACTTTTGGAACGCGTAGCAACTCTGGTAAGGGCAAATCTCAACGATCTCGTGGACAAGGCCGAAGACCCGGAGAA
AATGATCAAGCAGGTGATCCTGGACATGCAGAATCAGCTCCTGCAGGTCAAAACCCAGGTCGCGATCACCATCGCCGACC
AACACGTGCTCGAGAAGAAGCAGCAGGAAGCGGAAGAGAACGAGCGTCAGTGGATGCACCGCGCCGGTCTCGCGGTGGAC
AAGAAGGACGACGCCCTGGCGCGCGCCGCCGTGGAACGCAGCATGAGCTACAAGGCCACCGCCGCCAATTTCCGCCAGCA
GGTGGAGGATCAGAAGGGTCAGGTCGAAAACCTGAAATCCGCGCTCCTCAAACTGCAGACCAAATTGGCCGAAGCGCAAT
CGAAGAGCGATGTGCTGATCGCGCAACACCGGCGCGCCCGGGCCATGAACCGGGCCAACCAGGCCACCAGCGGGCTCGGC
GATAACGCCAACTCCGAGGCGTTCGACCGCATGAAGAGCAAGGTCCAGCACCACGAAGCCACCGCGCAGGCGCATGCCGA
ACTGGTCAGCGACGACGTAAACGATAAATTCGCTGCGATGGAAAAGCAGGAGGAAATCGATCGCCTGCTCAGTGATCTGA
AATCGAAGCGTAAAGTCTGA

Upstream 100 bases:

>100_bases
CAAGGTGAGAACCATCGGCGAACAAGCGCCCAAACCGGACGACAAAGTGAAAGGCGCAGGAGCGTAGTCAGGCAATCGCA
GGGTAATAAGGAGCAAGACA

Downstream 100 bases:

>100_bases
ACCCGCGGGGCAGGCCATCCGGCCTGTCCCGCCCCAACCACAAAATCAAAATTTATATGAGACATACATCGAAGGTCGAA
GTGTGGCTGTTCGCGGCGAT

Product: phage shock protein PspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MALLERVATLVRANLNDLVDKAEDPEKMIKQVILDMQNQLLQVKTQVAITIADQHVLEKKQQEAEENERQWMHRAGLAVD
KKDDALARAAVERSMSYKATAANFRQQVEDQKGQVENLKSALLKLQTKLAEAQSKSDVLIAQHRRARAMNRANQATSGLG
DNANSEAFDRMKSKVQHHEATAQAHAELVSDDVNDKFAAMEKQEEIDRLLSDLKSKRKV

Sequences:

>Translated_219_residues
MALLERVATLVRANLNDLVDKAEDPEKMIKQVILDMQNQLLQVKTQVAITIADQHVLEKKQQEAEENERQWMHRAGLAVD
KKDDALARAAVERSMSYKATAANFRQQVEDQKGQVENLKSALLKLQTKLAEAQSKSDVLIAQHRRARAMNRANQATSGLG
DNANSEAFDRMKSKVQHHEATAQAHAELVSDDVNDKFAAMEKQEEIDRLLSDLKSKRKV
>Mature_218_residues
ALLERVATLVRANLNDLVDKAEDPEKMIKQVILDMQNQLLQVKTQVAITIADQHVLEKKQQEAEENERQWMHRAGLAVDK
KDDALARAAVERSMSYKATAANFRQQVEDQKGQVENLKSALLKLQTKLAEAQSKSDVLIAQHRRARAMNRANQATSGLGD
NANSEAFDRMKSKVQHHEATAQAHAELVSDDVNDKFAAMEKQEEIDRLLSDLKSKRKV

Specific function: The Phage Shock Protein (Psp) Operon (Pspabce) May Play A Significant Role In The Competition For Survival Under Nutrient- Or Energy-Limited Conditions. Pspa Has A Role As A Negative Regulator, Is Required For Efficient Translocation And Has An Unknown Fu

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=221, Percent_Identity=28.5067873303167, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 24678; Mature: 24547

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLERVATLVRANLNDLVDKAEDPEKMIKQVILDMQNQLLQVKTQVAITIADQHVLEKK
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQEAEENERQWMHRAGLAVDKKDDALARAAVERSMSYKATAANFRQQVEDQKGQVENLKS
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLKLQTKLAEAQSKSDVLIAQHRRARAMNRANQATSGLGDNANSEAFDRMKSKVQHHEA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TAQAHAELVSDDVNDKFAAMEKQEEIDRLLSDLKSKRKV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALLERVATLVRANLNDLVDKAEDPEKMIKQVILDMQNQLLQVKTQVAITIADQHVLEKK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQEAEENERQWMHRAGLAVDKKDDALARAAVERSMSYKATAANFRQQVEDQKGQVENLKS
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLKLQTKLAEAQSKSDVLIAQHRRARAMNRANQATSGLGDNANSEAFDRMKSKVQHHEA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TAQAHAELVSDDVNDKFAAMEKQEEIDRLLSDLKSKRKV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]