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The map label for this gene is macA [H]

Identifier: 116623649

GI number: 116623649

Start: 5764733

End: 5766076

Strand: Reverse

Name: macA [H]

Synonym: Acid_4561

Alternate gene names: 116623649

Gene position: 5766076-5764733 (Counterclockwise)

Preceding gene: 116623650

Following gene: 116623648

Centisome position: 57.86

GC content: 64.96

Gene sequence:

>1344_bases
ATGAACGACACTCATTCCACCGAAGACCGCTTGCGCTCTGAGATCGAGGATCTCAAGCGGCAGTTGGAAAGCGCGAAGCA
TCCGGGGGCGCCCGCTGACCGGCGTCCTTCGGCCGGCACGCTGATCCTGATCGCCCTATTGCTCACCGTGCTCATCGTCG
CCGGCTTTTTCGTCGGCTACCTGCCAAGGCAGAAACGCGAGGACGTCCTCGCCGCCGAATCGAAGGAGACCAGCAGCAGC
CTGCCGCTGGTTAACGTCTCCAAGGTCACGCGCGCCGCTGGGCGCAGCAACCTTGTTCTGCCCGGCAACATTCAGGCAGT
CACGGAAGCCCCCATTCTGGCCCGCGCCACGGGATACATCAAGAAGCGCTATGTCGATATCGGCGACCGCGTGAAGTCCG
GCCAGGTGGTGGCCGACATCGAAGCGCCGGAGCTGGACCAGCAGATTCTGCAAGCCCGCGCGGTCATCGAACAGGCCAAG
AGCAGCGTGCAGCAGGCCGACGCCGCCATTCAACAGGCACGCGCCAATGAGAACCTCGCCAAGGTCACCGCCGGACGCAT
GAGCAACCTGCTGGGGCGCGGCGTTATTTCGAAACAGGACAACGATACCGCCCAGGCGCAGTACGCGGCGCAGCAGGCCA
ACGTCCAGGCCCTGGAACAGGCATCCGCGGCCGCGCGCAGCAACGTCTCCGCGGCCGAAGCCAACCTCTCGCGGCTCAAT
CAGCTCAAGACGTACCAGACTGTGCGCGCCCCCTTCGATGGCGTCATCACGCTGCGCAACGTGGATACCGGCGCGCTGGT
CAACGAAGGCAGCACGCTGCTCTTCCGCATCGCCCAGACCGGGACGCTGCGAATCTATGTAAACCTTCCGCAGTCCGAAG
CCGACTCGATCAAGCCGGGGCAGCGCGCCGATATCTCGATCGCGGAGCTCATCGGCAAGAAATTCACCGGCGTGGTGACG
CGCACGTCGAACGCGCTCGACCCCGCCACCCGGACGCTGCTCACCGAAGTCCAGGTGCCGAATTCGGGCGGCGCGCTGAT
GCCGGGTATGTATGCCAACGTGGATCTCATCGTGCCGCGCAAAGATCCGCCGCTGCTGATTCCCGGCGATACCCTGGTAG
TGCGCGCCGACGGACCGCAGGTCGCGGTAGTGCAACCCGACTCGACCGTCCATTTCACGCGCATCACGCTGGGCCGCGAT
TTCGGCGATCGGCTGGAAGTGCTCGGCGGCGGCCTGGAACTGGGCCAGCAGCTCGCCGTCAACCCGAGCGATGTAGTTCG
TGAAGGCGCCAAGGTCAAGCCTGTGGCGGCCCCTGAAAAAGCCCCGGCGGGCAAGAAGCAGTAG

Upstream 100 bases:

>100_bases
GCCTGCTGCGTAAGAATCCCCCGGTGGATTTCGAACGCAGGATTGTGGAAGAGGAGCACGAATATTTGCGCGACGAAATG
ATGCTGCGTGAGGACCTTTC

Downstream 100 bases:

>100_bases
GGACCCGAAAATGTTTTACAAGCCTGCCTGCGCCGCCCTGGCGCTGTTCCTCGCCGCCGATCTCGGCCTCGCCCAGACCG
GCATACCGCGCATGACCCCG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNDTHSTEDRLRSEIEDLKRQLESAKHPGAPADRRPSAGTLILIALLLTVLIVAGFFVGYLPRQKREDVLAAESKETSSS
LPLVNVSKVTRAAGRSNLVLPGNIQAVTEAPILARATGYIKKRYVDIGDRVKSGQVVADIEAPELDQQILQARAVIEQAK
SSVQQADAAIQQARANENLAKVTAGRMSNLLGRGVISKQDNDTAQAQYAAQQANVQALEQASAAARSNVSAAEANLSRLN
QLKTYQTVRAPFDGVITLRNVDTGALVNEGSTLLFRIAQTGTLRIYVNLPQSEADSIKPGQRADISIAELIGKKFTGVVT
RTSNALDPATRTLLTEVQVPNSGGALMPGMYANVDLIVPRKDPPLLIPGDTLVVRADGPQVAVVQPDSTVHFTRITLGRD
FGDRLEVLGGGLELGQQLAVNPSDVVREGAKVKPVAAPEKAPAGKKQ

Sequences:

>Translated_447_residues
MNDTHSTEDRLRSEIEDLKRQLESAKHPGAPADRRPSAGTLILIALLLTVLIVAGFFVGYLPRQKREDVLAAESKETSSS
LPLVNVSKVTRAAGRSNLVLPGNIQAVTEAPILARATGYIKKRYVDIGDRVKSGQVVADIEAPELDQQILQARAVIEQAK
SSVQQADAAIQQARANENLAKVTAGRMSNLLGRGVISKQDNDTAQAQYAAQQANVQALEQASAAARSNVSAAEANLSRLN
QLKTYQTVRAPFDGVITLRNVDTGALVNEGSTLLFRIAQTGTLRIYVNLPQSEADSIKPGQRADISIAELIGKKFTGVVT
RTSNALDPATRTLLTEVQVPNSGGALMPGMYANVDLIVPRKDPPLLIPGDTLVVRADGPQVAVVQPDSTVHFTRITLGRD
FGDRLEVLGGGLELGQQLAVNPSDVVREGAKVKPVAAPEKAPAGKKQ
>Mature_447_residues
MNDTHSTEDRLRSEIEDLKRQLESAKHPGAPADRRPSAGTLILIALLLTVLIVAGFFVGYLPRQKREDVLAAESKETSSS
LPLVNVSKVTRAAGRSNLVLPGNIQAVTEAPILARATGYIKKRYVDIGDRVKSGQVVADIEAPELDQQILQARAVIEQAK
SSVQQADAAIQQARANENLAKVTAGRMSNLLGRGVISKQDNDTAQAQYAAQQANVQALEQASAAARSNVSAAEANLSRLN
QLKTYQTVRAPFDGVITLRNVDTGALVNEGSTLLFRIAQTGTLRIYVNLPQSEADSIKPGQRADISIAELIGKKFTGVVT
RTSNALDPATRTLLTEVQVPNSGGALMPGMYANVDLIVPRKDPPLLIPGDTLVVRADGPQVAVVQPDSTVHFTRITLGRD
FGDRLEVLGGGLELGQQLAVNPSDVVREGAKVKPVAAPEKAPAGKKQ

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=386, Percent_Identity=26.4248704663212, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082036, Length=343, Percent_Identity=24.4897959183673, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081796, Length=274, Percent_Identity=23.7226277372263, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786668, Length=378, Percent_Identity=26.984126984127, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 47651; Mature: 47651

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTHSTEDRLRSEIEDLKRQLESAKHPGAPADRRPSAGTLILIALLLTVLIVAGFFVGY
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LPRQKREDVLAAESKETSSSLPLVNVSKVTRAAGRSNLVLPGNIQAVTEAPILARATGYI
CCCHHHHHHHHCCCCCCCCCCCEEEHHHHHHHCCCCCEEECCCCCCHHCCCHHHHHHHHH
KKRYVDIGDRVKSGQVVADIEAPELDQQILQARAVIEQAKSSVQQADAAIQQARANENLA
HHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KVTAGRMSNLLGRGVISKQDNDTAQAQYAAQQANVQALEQASAAARSNVSAAEANLSRLN
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QLKTYQTVRAPFDGVITLRNVDTGALVNEGSTLLFRIAQTGTLRIYVNLPQSEADSIKPG
HHHHHHHHHCCCCCEEEEEECCCCCEECCCCEEEEEEECCCEEEEEEECCCCCCCCCCCC
QRADISIAELIGKKFTGVVTRTSNALDPATRTLLTEVQVPNSGGALMPGMYANVDLIVPR
CCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEEC
KDPPLLIPGDTLVVRADGPQVAVVQPDSTVHFTRITLGRDFGDRLEVLGGGLELGQQLAV
CCCCEEECCCEEEEEECCCEEEEECCCCCEEEEEEEECCCCHHHHHHHCCCHHHCHHHCC
NPSDVVREGAKVKPVAAPEKAPAGKKQ
CHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNDTHSTEDRLRSEIEDLKRQLESAKHPGAPADRRPSAGTLILIALLLTVLIVAGFFVGY
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LPRQKREDVLAAESKETSSSLPLVNVSKVTRAAGRSNLVLPGNIQAVTEAPILARATGYI
CCCHHHHHHHHCCCCCCCCCCCEEEHHHHHHHCCCCCEEECCCCCCHHCCCHHHHHHHHH
KKRYVDIGDRVKSGQVVADIEAPELDQQILQARAVIEQAKSSVQQADAAIQQARANENLA
HHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KVTAGRMSNLLGRGVISKQDNDTAQAQYAAQQANVQALEQASAAARSNVSAAEANLSRLN
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QLKTYQTVRAPFDGVITLRNVDTGALVNEGSTLLFRIAQTGTLRIYVNLPQSEADSIKPG
HHHHHHHHHCCCCCEEEEEECCCCCEECCCCEEEEEEECCCEEEEEEECCCCCCCCCCCC
QRADISIAELIGKKFTGVVTRTSNALDPATRTLLTEVQVPNSGGALMPGMYANVDLIVPR
CCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEEC
KDPPLLIPGDTLVVRADGPQVAVVQPDSTVHFTRITLGRDFGDRLEVLGGGLELGQQLAV
CCCCEEECCCEEEEEECCCEEEEECCCCCEEEEEEEECCCCHHHHHHHCCCHHHCHHHCC
NPSDVVREGAKVKPVAAPEKAPAGKKQ
CHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]