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The map label for this gene is argG

Identifier: 116623538

GI number: 116623538

Start: 5630146

End: 5631507

Strand: Reverse

Name: argG

Synonym: Acid_4448

Alternate gene names: 116623538

Gene position: 5631507-5630146 (Counterclockwise)

Preceding gene: 116623539

Following gene: 116623537

Centisome position: 56.51

GC content: 62.04

Gene sequence:

>1362_bases
ATGGGAAACATTCTGCAGAACCTGCCCGCCGGCGAAAAGGTCGGTCTGGCCTTCTCCGGCGGCCTCGATACCAGCGCCGC
GATTCATTGGATGCGCGCTAAAGGCGCGATCCCGTATTCGTACACCGCCAATCTCGGACAGCCCGACGAGCCGGATTACG
ACGAGATCCCCCGCCGCGCGCTGCTCTACGGCGCCGAGAAAGCGCGGCTCATCGATTGCCGCAAGCAGCTTGTGGCCGAA
GGCATCGCCGCCCTGCAATCCGGCGCTTTCCACATCTCGACCGCCGGAGTCCACTACTTCAATACGACACCCATTGGCCG
CGCGGTCACGGGCACCATGCTGGTGATCGCGATGAAGGAGGACGACGTCCACATCTGGGGCGATGGCAGCACCTTCAAGG
GCAACGATATCGAGCGTTTCTACCGCTACGGCCTGCTGGCCAATCCCGCGCTGCGCATCTACAAGCCGTGGCTTGACCAG
ACCTTCATCGACGAACTCGGCGGCCGCAAGGAAATGTCGGAGTACATGATCTCGCACGGGCTCACCTATAAGATGAGCGT
GGAGAAGGCTTACTCGACCGATTCCAACATCTGGGGCGCAACTCACGAAGCCAAGGATCTCGAGCACCTCGATAAGGGCG
TCAAGATCGTGGAGCCCATCATGGGCGTCGCCTCGTGGCGCGAAGACGTCGCGATTAAACCCGAAACTGTCACTATCCGT
TTCGAAGAGGGCCAGCCCGTGGCGCTCAACCACGTGACGTATGACGACCCGGTGCAACTCGTGCTCGAAGCCAACGCGAT
CGGCGGCCGCCACGGCCTCGGAGTATCCGACCAGATCGAGAATCGCATCATCGAAGCCAAGAGCCGGGGCATATACGAAG
CTCCCGGCCTTGCGCTGCTTTTCATTGCCTACGAGCGCCTGGTCTCCGGCATTCACAATGAGGACACGATCGAGCAGTAC
CGCATCAACGGCCTTCGCCTGGGGCGCCTGCTGTATCAGGGCCGCTGGTTCGATTCGCAATCCATGATGCTGCGGGAAAC
CGCACAGCGCTGGGTCGCGCGCGCGATCACGGGCGAGGTCACGGTCGAACTCCGCCGCGGCAACGACTACTCCATCCTCG
ACACCACCAGCCCCAACCTCACCTACAAGCCCGAGCGCCTCACGATGGAGAAAGGCGAATCGACTTTCTCTCCCCAGGAC
CGCATCGGCCAGCTCACAATGCGCCACCTGGACATCACCGACACGCGCGACAAGCTCCTGCTGTATATAAAGACCGGCCT
GCTCGGTCCATCCAGCGGCAATTCGACGACGCTGCTCCCCGAGCCCGCCGACAGTGGGACAGAGAAGCCGCCGAGCCGTT
GA

Upstream 100 bases:

>100_bases
ATGGGGATGGAAGTCGCACTCTCCGCCGACCGCGCGCCGCTTACGTTCGGCTATCTCGAAGTCCGCTGACTGCCGGAGTC
ACGCTATCCTAAATAATTCC

Downstream 100 bases:

>100_bases
TTGGCAGGTCATGATCAGAGTCGCACTGCCTTTTGGGAGCACGCCGGAAAGCAAACGCAGCCCCTACCGCCAGGCACTCG
CTCTCGCGGGCATTCAACCT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MGNILQNLPAGEKVGLAFSGGLDTSAAIHWMRAKGAIPYSYTANLGQPDEPDYDEIPRRALLYGAEKARLIDCRKQLVAE
GIAALQSGAFHISTAGVHYFNTTPIGRAVTGTMLVIAMKEDDVHIWGDGSTFKGNDIERFYRYGLLANPALRIYKPWLDQ
TFIDELGGRKEMSEYMISHGLTYKMSVEKAYSTDSNIWGATHEAKDLEHLDKGVKIVEPIMGVASWREDVAIKPETVTIR
FEEGQPVALNHVTYDDPVQLVLEANAIGGRHGLGVSDQIENRIIEAKSRGIYEAPGLALLFIAYERLVSGIHNEDTIEQY
RINGLRLGRLLYQGRWFDSQSMMLRETAQRWVARAITGEVTVELRRGNDYSILDTTSPNLTYKPERLTMEKGESTFSPQD
RIGQLTMRHLDITDTRDKLLLYIKTGLLGPSSGNSTTLLPEPADSGTEKPPSR

Sequences:

>Translated_453_residues
MGNILQNLPAGEKVGLAFSGGLDTSAAIHWMRAKGAIPYSYTANLGQPDEPDYDEIPRRALLYGAEKARLIDCRKQLVAE
GIAALQSGAFHISTAGVHYFNTTPIGRAVTGTMLVIAMKEDDVHIWGDGSTFKGNDIERFYRYGLLANPALRIYKPWLDQ
TFIDELGGRKEMSEYMISHGLTYKMSVEKAYSTDSNIWGATHEAKDLEHLDKGVKIVEPIMGVASWREDVAIKPETVTIR
FEEGQPVALNHVTYDDPVQLVLEANAIGGRHGLGVSDQIENRIIEAKSRGIYEAPGLALLFIAYERLVSGIHNEDTIEQY
RINGLRLGRLLYQGRWFDSQSMMLRETAQRWVARAITGEVTVELRRGNDYSILDTTSPNLTYKPERLTMEKGESTFSPQD
RIGQLTMRHLDITDTRDKLLLYIKTGLLGPSSGNSTTLLPEPADSGTEKPPSR
>Mature_452_residues
GNILQNLPAGEKVGLAFSGGLDTSAAIHWMRAKGAIPYSYTANLGQPDEPDYDEIPRRALLYGAEKARLIDCRKQLVAEG
IAALQSGAFHISTAGVHYFNTTPIGRAVTGTMLVIAMKEDDVHIWGDGSTFKGNDIERFYRYGLLANPALRIYKPWLDQT
FIDELGGRKEMSEYMISHGLTYKMSVEKAYSTDSNIWGATHEAKDLEHLDKGVKIVEPIMGVASWREDVAIKPETVTIRF
EEGQPVALNHVTYDDPVQLVLEANAIGGRHGLGVSDQIENRIIEAKSRGIYEAPGLALLFIAYERLVSGIHNEDTIEQYR
INGLRLGRLLYQGRWFDSQSMMLRETAQRWVARAITGEVTVELRRGNDYSILDTTSPNLTYKPERLTMEKGESTFSPQDR
IGQLTMRHLDITDTRDKLLLYIKTGLLGPSSGNSTTLLPEPADSGTEKPPSR

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=410, Percent_Identity=29.2682926829268, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI16950633, Length=410, Percent_Identity=29.2682926829268, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI1789563, Length=434, Percent_Identity=76.036866359447, Blast_Score=692, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=417, Percent_Identity=29.0167865707434, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21358151, Length=416, Percent_Identity=27.1634615384615, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_SOLUE (Q01Y56)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_825694.1
- ProteinModelPortal:   Q01Y56
- SMR:   Q01Y56
- STRING:   Q01Y56
- GeneID:   4431976
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_4448
- NMPDR:   fig|234267.9.peg.4232
- eggNOG:   COG0137
- HOGENOM:   HBG289560
- OMA:   GGRKEMS
- PhylomeDB:   Q01Y56
- ProtClustDB:   PRK05370
- GO:   GO:0005737
- HAMAP:   MF_00581
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 50433; Mature: 50302

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 43-43 BINDING 99-99 BINDING 129-129 BINDING 131-131 BINDING 131-131 BINDING 135-135 BINDING 135-135 BINDING 136-136 BINDING 136-136 BINDING 139-139 BINDING 192-192 BINDING 194-194 BINDING 201-201 BINDING 203-203 BINDING 280-280

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNILQNLPAGEKVGLAFSGGLDTSAAIHWMRAKGAIPYSYTANLGQPDEPDYDEIPRRA
CCCHHHHCCCCCEEEEEEECCCCCHHHEEEEHHCCCCCEEEECCCCCCCCCCHHHHCCHH
LLYGAEKARLIDCRKQLVAEGIAALQSGAFHISTAGVHYFNTTPIGRAVTGTMLVIAMKE
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCEEECEEEEEEEEC
DDVHIWGDGSTFKGNDIERFYRYGLLANPALRIYKPWLDQTFIDELGGRKEMSEYMISHG
CCEEEEECCCCCCCCCHHHHHHHCCCCCCHHHEEHHHHHHHHHHHHCCHHHHHHHHHHCC
LTYKMSVEKAYSTDSNIWGATHEAKDLEHLDKGVKIVEPIMGVASWREDVAIKPETVTIR
CEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCEECCCEEEEE
FEEGQPVALNHVTYDDPVQLVLEANAIGGRHGLGVSDQIENRIIEAKSRGIYEAPGLALL
ECCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
FIAYERLVSGIHNEDTIEQYRINGLRLGRLLYQGRWFDSQSMMLRETAQRWVARAITGEV
HHHHHHHHHCCCCCHHHHHHHHCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEE
TVELRRGNDYSILDTTSPNLTYKPERLTMEKGESTFSPQDRIGQLTMRHLDITDTRDKLL
EEEEECCCCEEEEECCCCCCEECCCHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCCCEE
LYIKTGLLGPSSGNSTTLLPEPADSGTEKPPSR
EEEEECCCCCCCCCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
GNILQNLPAGEKVGLAFSGGLDTSAAIHWMRAKGAIPYSYTANLGQPDEPDYDEIPRRA
CCHHHHCCCCCEEEEEEECCCCCHHHEEEEHHCCCCCEEEECCCCCCCCCCHHHHCCHH
LLYGAEKARLIDCRKQLVAEGIAALQSGAFHISTAGVHYFNTTPIGRAVTGTMLVIAMKE
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCEEECEEEEEEEEC
DDVHIWGDGSTFKGNDIERFYRYGLLANPALRIYKPWLDQTFIDELGGRKEMSEYMISHG
CCEEEEECCCCCCCCCHHHHHHHCCCCCCHHHEEHHHHHHHHHHHHCCHHHHHHHHHHCC
LTYKMSVEKAYSTDSNIWGATHEAKDLEHLDKGVKIVEPIMGVASWREDVAIKPETVTIR
CEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCEECCCEEEEE
FEEGQPVALNHVTYDDPVQLVLEANAIGGRHGLGVSDQIENRIIEAKSRGIYEAPGLALL
ECCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
FIAYERLVSGIHNEDTIEQYRINGLRLGRLLYQGRWFDSQSMMLRETAQRWVARAITGEV
HHHHHHHHHCCCCCHHHHHHHHCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEE
TVELRRGNDYSILDTTSPNLTYKPERLTMEKGESTFSPQDRIGQLTMRHLDITDTRDKLL
EEEEECCCCEEEEECCCCCCEECCCHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCCCEE
LYIKTGLLGPSSGNSTTLLPEPADSGTEKPPSR
EEEEECCCCCCCCCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA