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The map label for this gene is paaF [H]

Identifier: 116623518

GI number: 116623518

Start: 5602442

End: 5603212

Strand: Reverse

Name: paaF [H]

Synonym: Acid_4428

Alternate gene names: 116623518

Gene position: 5603212-5602442 (Counterclockwise)

Preceding gene: 116623519

Following gene: 116623517

Centisome position: 56.23

GC content: 64.2

Gene sequence:

>771_bases
ATGACCGATTTGCTTCATTCCGTTCGCGAGGGACGTGTTCTGCGTTTGACCCTCAATCGACCCGAGAAACGAAATGCTTT
AGACGCAGCGCTTTGCCGAGCTCTGGTGGATACATTAGAGGCGGCGGAACATGAGGCCGCAGTGGGGGCGATCCTGCTGG
CGGCGAACGGCAAGGCCTTCTGCGCCGGGATGGATCTCAGCGAAATCGGGCAGGTAGGCGACACCAGCGAGATCAATAAG
GTGCACGAGAGGCTTTTCACGGCGGGGGCGCGGCTGACCAAACCGATCGTGGCGGCGGTGCATGGCCCGGCCATCGGCGG
GGGCACTGGCCTGGTGGCGAACTGCCACATTGTGGTGGCGGGATCGGAGGCGACGTTCGGGCTGACCGAAATTCGGCTGG
GGCTATGGCCGTTCCTGATTTATCGTCCCGTTACGGCCGCGCTTGGGGAGCGGCGCACGCTCGAACTGGCACTGACCGGC
CGGGTCTTCGGCGCGGCGGAGGCCCGGGAGATGCTGCTGGTACACGAGGTCGCCGGTGATCCGCTCGCACGTGCAACGGA
GATTGCGGCGGCGGTCGCCGAGTTCAGTCCCATGGCGATTCGAAGCGGGCTTCAGTTCGTCCGGGAGGTGCGGGACGTGG
ATTCCGCGCGCGCTGTTGAGGTTGCGCAAGCGGTGCGGAATGAAGTATTTGCCGGCGCAGACTTCAAGGAAGGGATCCGC
GCCTTCCATGAGAAGCGGAGGCCCCGATGGCCGTCGATCTCGAAGGCGTAA

Upstream 100 bases:

>100_bases
CTGGCAGACACGTTGAGGAAACTGGCGACAGAGATCTGAGAGTATAATTGTGGCAACGGCGTGCTCCGCGCCGGGCGAAC
CCTCTCCGCACATCTGCGTA

Downstream 100 bases:

>100_bases
CTTCAATTTTGCGGATATGCGACACTAGATCTTGTGGTGTCGTGGTCAAGGGCACTCAATGGTGTGGTGGAGGTACTTTA
CTTTTGCGCCAAGCGCGTTT

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTDLLHSVREGRVLRLTLNRPEKRNALDAALCRALVDTLEAAEHEAAVGAILLAANGKAFCAGMDLSEIGQVGDTSEINK
VHERLFTAGARLTKPIVAAVHGPAIGGGTGLVANCHIVVAGSEATFGLTEIRLGLWPFLIYRPVTAALGERRTLELALTG
RVFGAAEAREMLLVHEVAGDPLARATEIAAAVAEFSPMAIRSGLQFVREVRDVDSARAVEVAQAVRNEVFAGADFKEGIR
AFHEKRRPRWPSISKA

Sequences:

>Translated_256_residues
MTDLLHSVREGRVLRLTLNRPEKRNALDAALCRALVDTLEAAEHEAAVGAILLAANGKAFCAGMDLSEIGQVGDTSEINK
VHERLFTAGARLTKPIVAAVHGPAIGGGTGLVANCHIVVAGSEATFGLTEIRLGLWPFLIYRPVTAALGERRTLELALTG
RVFGAAEAREMLLVHEVAGDPLARATEIAAAVAEFSPMAIRSGLQFVREVRDVDSARAVEVAQAVRNEVFAGADFKEGIR
AFHEKRRPRWPSISKA
>Mature_255_residues
TDLLHSVREGRVLRLTLNRPEKRNALDAALCRALVDTLEAAEHEAAVGAILLAANGKAFCAGMDLSEIGQVGDTSEINKV
HERLFTAGARLTKPIVAAVHGPAIGGGTGLVANCHIVVAGSEATFGLTEIRLGLWPFLIYRPVTAALGERRTLELALTGR
VFGAAEAREMLLVHEVAGDPLARATEIAAAVAEFSPMAIRSGLQFVREVRDVDSARAVEVAQAVRNEVFAGADFKEGIRA
FHEKRRPRWPSISKA

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=238, Percent_Identity=30.2521008403361, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI70995211, Length=264, Percent_Identity=29.5454545454545, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=27.4193548387097, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI37594471, Length=238, Percent_Identity=28.9915966386555, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI37594469, Length=238, Percent_Identity=28.9915966386555, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=29.1828793774319, Blast_Score=68, Evalue=6e-12,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=34.8659003831418, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=27.3764258555133, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=30.9433962264151, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=28.3018867924528, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082183, Length=248, Percent_Identity=27.0161290322581, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=33.0612244897959, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=32.1428571428571, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17536985, Length=256, Percent_Identity=28.90625, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=26.0330578512397, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=234, Percent_Identity=28.2051282051282, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=217, Percent_Identity=24.4239631336406, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=201, Percent_Identity=26.3681592039801, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=239, Percent_Identity=26.7782426778243, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=231, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=231, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=31.6939890710383, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129971, Length=242, Percent_Identity=33.0578512396694, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653477, Length=242, Percent_Identity=33.0578512396694, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=28.4090909090909, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357171, Length=259, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920382, Length=209, Percent_Identity=28.2296650717703, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=27.1653543307087, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650670, Length=198, Percent_Identity=31.3131313131313, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27388; Mature: 27257

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLLHSVREGRVLRLTLNRPEKRNALDAALCRALVDTLEAAEHEAAVGAILLAANGKAF
CCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEE
CAGMDLSEIGQVGDTSEINKVHERLFTAGARLTKPIVAAVHGPAIGGGTGLVANCHIVVA
EECCCHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEE
GSEATFGLTEIRLGLWPFLIYRPVTAALGERRTLELALTGRVFGAAEAREMLLVHEVAGD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCC
PLARATEIAAAVAEFSPMAIRSGLQFVREVRDVDSARAVEVAQAVRNEVFAGADFKEGIR
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHH
AFHEKRRPRWPSISKA
HHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TDLLHSVREGRVLRLTLNRPEKRNALDAALCRALVDTLEAAEHEAAVGAILLAANGKAF
CHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEE
CAGMDLSEIGQVGDTSEINKVHERLFTAGARLTKPIVAAVHGPAIGGGTGLVANCHIVVA
EECCCHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEE
GSEATFGLTEIRLGLWPFLIYRPVTAALGERRTLELALTGRVFGAAEAREMLLVHEVAGD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCC
PLARATEIAAAVAEFSPMAIRSGLQFVREVRDVDSARAVEVAQAVRNEVFAGADFKEGIR
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHH
AFHEKRRPRWPSISKA
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]