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The map label for this gene is ysaA [H]

Identifier: 116623401

GI number: 116623401

Start: 5458190

End: 5460457

Strand: Direct

Name: ysaA [H]

Synonym: Acid_4310

Alternate gene names: 116623401

Gene position: 5458190-5460457 (Clockwise)

Preceding gene: 116623395

Following gene: 116623402

Centisome position: 54.77

GC content: 62.35

Gene sequence:

>2268_bases
ATGGCTAAGGACGTCATCCTAACTGCCGATTTGGGTGGCGACCTGCTGACGGCAGAGGAGCTCGCCGCCATTCCCGATTT
CGCCGGCATACGCAAGGAAATCTGGGACAAGTTCCCGGGCGCCATCGCGCGCAAGAAATACAGCCCCGGCGAGATCCTGA
TGCGCGAGGGGGAGAACGGCACCACGGCCTTCTACATCCTCTCCGGCACCATCGAGCTTTTCATTAACAATCCGGTCTCT
CACGTTGAAAGCTCAAGAGGTTCCAGCGGGAGCTGGCTGGGCGGCCTGACCAAGATCACCAACTACATCAAAGGCGTCCC
GGCACCCAAAAGCGCGGGAGGACACGGACGTACCCACATCCCCATCGACGCATCGGTGGACCTGCCGCTGGAGAACCCGA
TAGCCGAAGTTACCAGCGGCGATCTGATCGGCGAACTGGCCGCGCTCGCCGCCCTTAAGCAGGAGCGGCTTAAACGTCCG
AAATTCTATCCGCGCTCCGCCACGGCTCGCGCCAAGAGTGAGGTGGTGGTGCTCGAGATGTTGCCGAACATCTTGAATAA
TGTTCTCTATAACGCGGCATCCTTCAAGGAAAGATTAAATAAAAACTACCGGCTGCGCGCGCTCGATAGCCACCTGCGCA
CGGTACCGGTCTTCCGTAATCTCAGCGCCGATTTCCTGGAATATCTGCGCGATCGCGTTGAACTGGTTGACGCCGTTCCA
GGTCAGGTGATCTGCCGGCAGGGCGAGATCGCCGATTCCTTTTACCTGATCCGCATGGGCTTTGTGAAGGTTTCCCAGGT
CTTCCCCGGCGGCGAACTGGTGCTGACCTATCTCTCGCGCGGCTCCTACTTCGGCGAAATGGGACTCCTGCCGCCGGTCT
TCCGCATTCGCGCCAAAGGTGTGAAGCCCGGGAACCTCGCCGAGGTCCCCGTCTCCAGCGCGGAGGTCACGGTTGGCCGC
GCACCGAACTCCGGGGGCGCAATGCCGATTCCCTGGGACGAGTACATCTCGCGCGAACACGCCACTATGCGCGTGGAGGG
TAGACAACTGCGCGTCAGCCGCATGGGCTCCGGCAAAAATCCGATTACCTTCCGCATGAGGCCGGTGGAAACCGCCCTGC
TCTCTCCGGGCGAGAGCTTCGTCATCGGCGAAACTACCTTCGAAGTGGTCGAAGATCCGCTCCAGTCGGGACGCCGCACC
GCCACTTGCACTGCCGTCGATTTCGTTCAGTTAGTACGGATTAAGGCCGAAGACTTCGCGCAAATGTTGGAGCGCTTCCC
GGAGGTCTCAACCGGCATTAATGAAGTCGCGCGCGCCCGCCGCGAGATGGATTTGCATCTGCTCGGCCGCGTGCAGACTG
CCTCGCTTTCGAGCTTCCTCGAGCAGGAGTTGATGCAGGGCCAGAACCTCCTGCTCCTGGATTTGGACAAATGCACGCGC
TGCGACGAGTGCGTGAAGGCCTGCGTCGCCACGCACAACGACGGCGTCACCAGACTGGTGCGCGATGGCTTGCGCTTCGA
AAATTTCCTGGTGGCCACCAGCTGCCGCGCCTGCATGGATCCCCTGTGCATGACGCGCTGCCCGGTCGGCTCCATCCGCC
GCAAGGATTCGCTCGATATCGTGATCGAGGACTGGTGCATCGGCTGCGGCAACTGCGCCATCGATTGCCCCTACGGCAAC
ATCAACGTGGTGGAGGTGCAGGAGACCAAACGCAAGCAGAAGGCCGAGCCGCGCCCCAAGGCCGTGGTCTGCGACCTGTG
CGCGGAGTTCGCCGAGCCCAACTGCGTTCGCGCCTGCCCTCACGATGCCGCCATCCGGGTGGAGCCCAAGACCTTCTTCG
CGCGCGACCTGGCCGGTATGCAGCTCATCGTGCCGGCCGGCGCCCCCGCCCCGCTGCCGGTCGCGCCGGCGGAGCCCGAG
AACATCGAGACCAAAATCTACTCCAACGTCGGCGAGTTGCTCAACATGCTGCCGCGGCTCAAGATCGTCTCCGGCCCGCG
CTCGGGATCGGTCCTGCAGCTGCGATTCCCCACCACCACCTTCGGCCGCTCCGCCGACAACGACTACCGGTTCGCCGACG
ATACCCTGATGTCGCGCGCCCAGGCCGTGATCCTCTGCGAAGGCAGCCGCTTCGTGCTGCGCGACCTGAACTCCACCAAT
GGCACTCTGGTCAACGGCAACGCCATCACCGAGATCGAGCTTCATACCGGCGACGTGATCGAGATGGGTGAGATGCAGAT
GGAATTCCTGGGAGGCCAGGTCCAATGA

Upstream 100 bases:

>100_bases
GCAAGGTGGTGGTTATCCCCGCAGTTAAAGATTTCGCCAATCATGTTATGAAATCTGACATACTAGCTCCAATTGACGGG
TGGAAATCAACAGGTAATCA

Downstream 100 bases:

>100_bases
CGCTGGATCGCCGGCAGACGAAGTGGGCGGTGGGCAGCGGTATCGGCTCGGCCGCCTCGCTGGCTGCATATATCGCGTAC
GTCCTGGTCTCGCCGAATGG

Product: cyclic nucleotide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MAKDVILTADLGGDLLTAEELAAIPDFAGIRKEIWDKFPGAIARKKYSPGEILMREGENGTTAFYILSGTIELFINNPVS
HVESSRGSSGSWLGGLTKITNYIKGVPAPKSAGGHGRTHIPIDASVDLPLENPIAEVTSGDLIGELAALAALKQERLKRP
KFYPRSATARAKSEVVVLEMLPNILNNVLYNAASFKERLNKNYRLRALDSHLRTVPVFRNLSADFLEYLRDRVELVDAVP
GQVICRQGEIADSFYLIRMGFVKVSQVFPGGELVLTYLSRGSYFGEMGLLPPVFRIRAKGVKPGNLAEVPVSSAEVTVGR
APNSGGAMPIPWDEYISREHATMRVEGRQLRVSRMGSGKNPITFRMRPVETALLSPGESFVIGETTFEVVEDPLQSGRRT
ATCTAVDFVQLVRIKAEDFAQMLERFPEVSTGINEVARARREMDLHLLGRVQTASLSSFLEQELMQGQNLLLLDLDKCTR
CDECVKACVATHNDGVTRLVRDGLRFENFLVATSCRACMDPLCMTRCPVGSIRRKDSLDIVIEDWCIGCGNCAIDCPYGN
INVVEVQETKRKQKAEPRPKAVVCDLCAEFAEPNCVRACPHDAAIRVEPKTFFARDLAGMQLIVPAGAPAPLPVAPAEPE
NIETKIYSNVGELLNMLPRLKIVSGPRSGSVLQLRFPTTTFGRSADNDYRFADDTLMSRAQAVILCEGSRFVLRDLNSTN
GTLVNGNAITEIELHTGDVIEMGEMQMEFLGGQVQ

Sequences:

>Translated_755_residues
MAKDVILTADLGGDLLTAEELAAIPDFAGIRKEIWDKFPGAIARKKYSPGEILMREGENGTTAFYILSGTIELFINNPVS
HVESSRGSSGSWLGGLTKITNYIKGVPAPKSAGGHGRTHIPIDASVDLPLENPIAEVTSGDLIGELAALAALKQERLKRP
KFYPRSATARAKSEVVVLEMLPNILNNVLYNAASFKERLNKNYRLRALDSHLRTVPVFRNLSADFLEYLRDRVELVDAVP
GQVICRQGEIADSFYLIRMGFVKVSQVFPGGELVLTYLSRGSYFGEMGLLPPVFRIRAKGVKPGNLAEVPVSSAEVTVGR
APNSGGAMPIPWDEYISREHATMRVEGRQLRVSRMGSGKNPITFRMRPVETALLSPGESFVIGETTFEVVEDPLQSGRRT
ATCTAVDFVQLVRIKAEDFAQMLERFPEVSTGINEVARARREMDLHLLGRVQTASLSSFLEQELMQGQNLLLLDLDKCTR
CDECVKACVATHNDGVTRLVRDGLRFENFLVATSCRACMDPLCMTRCPVGSIRRKDSLDIVIEDWCIGCGNCAIDCPYGN
INVVEVQETKRKQKAEPRPKAVVCDLCAEFAEPNCVRACPHDAAIRVEPKTFFARDLAGMQLIVPAGAPAPLPVAPAEPE
NIETKIYSNVGELLNMLPRLKIVSGPRSGSVLQLRFPTTTFGRSADNDYRFADDTLMSRAQAVILCEGSRFVLRDLNSTN
GTLVNGNAITEIELHTGDVIEMGEMQMEFLGGQVQ
>Mature_754_residues
AKDVILTADLGGDLLTAEELAAIPDFAGIRKEIWDKFPGAIARKKYSPGEILMREGENGTTAFYILSGTIELFINNPVSH
VESSRGSSGSWLGGLTKITNYIKGVPAPKSAGGHGRTHIPIDASVDLPLENPIAEVTSGDLIGELAALAALKQERLKRPK
FYPRSATARAKSEVVVLEMLPNILNNVLYNAASFKERLNKNYRLRALDSHLRTVPVFRNLSADFLEYLRDRVELVDAVPG
QVICRQGEIADSFYLIRMGFVKVSQVFPGGELVLTYLSRGSYFGEMGLLPPVFRIRAKGVKPGNLAEVPVSSAEVTVGRA
PNSGGAMPIPWDEYISREHATMRVEGRQLRVSRMGSGKNPITFRMRPVETALLSPGESFVIGETTFEVVEDPLQSGRRTA
TCTAVDFVQLVRIKAEDFAQMLERFPEVSTGINEVARARREMDLHLLGRVQTASLSSFLEQELMQGQNLLLLDLDKCTRC
DECVKACVATHNDGVTRLVRDGLRFENFLVATSCRACMDPLCMTRCPVGSIRRKDSLDIVIEDWCIGCGNCAIDCPYGNI
NVVEVQETKRKQKAEPRPKAVVCDLCAEFAEPNCVRACPHDAAIRVEPKTFFARDLAGMQLIVPAGAPAPLPVAPAEPEN
IETKIYSNVGELLNMLPRLKIVSGPRSGSVLQLRFPTTTFGRSADNDYRFADDTLMSRAQAVILCEGSRFVLRDLNSTNG
TLVNGNAITEIELHTGDVIEMGEMQMEFLGGQVQ

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367245, Length=148, Percent_Identity=30.4054054054054, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1788811, Length=142, Percent_Identity=32.3943661971831, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787122, Length=209, Percent_Identity=30.1435406698565, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789067, Length=154, Percent_Identity=33.7662337662338, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787872, Length=159, Percent_Identity=30.8176100628931, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 82881; Mature: 82750

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00888 CNMP_BINDING_1 ; PS50042 CNMP_BINDING_3 ; PS50006 FHA_DOMAIN ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDVILTADLGGDLLTAEELAAIPDFAGIRKEIWDKFPGAIARKKYSPGEILMREGENG
CCCCEEEEECCCCCEECHHHHHHCCCHHHHHHHHHHHCCCHHHHCCCCCCHHEEECCCCC
TTAFYILSGTIELFINNPVSHVESSRGSSGSWLGGLTKITNYIKGVPAPKSAGGHGRTHI
CEEEEEEEEEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
PIDASVDLPLENPIAEVTSGDLIGELAALAALKQERLKRPKFYPRSATARAKSEVVVLEM
ECCCEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHH
LPNILNNVLYNAASFKERLNKNYRLRALDSHLRTVPVFRNLSADFLEYLRDRVELVDAVP
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
GQVICRQGEIADSFYLIRMGFVKVSQVFPGGELVLTYLSRGSYFGEMGLLPPVFRIRAKG
CCEEECCCCCCCCEEEEHHHHHHHHHCCCCHHHEEEEHHCCCCCCCCCCCCHHHHHEECC
VKPGNLAEVPVSSAEVTVGRAPNSGGAMPIPWDEYISREHATMRVEGRQLRVSRMGSGKN
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCCEEEECCCEEEEEECCCCCC
PITFRMRPVETALLSPGESFVIGETTFEVVEDPLQSGRRTATCTAVDFVQLVRIKAEDFA
CEEEEEECHHHHHCCCCCCEEECCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
QMLERFPEVSTGINEVARARREMDLHLLGRVQTASLSSFLEQELMQGQNLLLLDLDKCTR
HHHHHCCHHHCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECHHCCC
CDECVKACVATHNDGVTRLVRDGLRFENFLVATSCRACMDPLCMTRCPVGSIRRKDSLDI
HHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCEE
VIEDWCIGCGNCAIDCPYGNINVVEVQETKRKQKAEPRPKAVVCDLCAEFAEPNCVRACP
EEECCCCCCCCEEEECCCCCEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEECCC
HDAAIRVEPKTFFARDLAGMQLIVPAGAPAPLPVAPAEPENIETKIYSNVGELLNMLPRL
CCCEEEECCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCE
KIVSGPRSGSVLQLRFPTTTFGRSADNDYRFADDTLMSRAQAVILCEGSRFVLRDLNSTN
EEEECCCCCCEEEEECCCCCCCCCCCCCCEECHHHHHCCCCEEEEECCCEEEEEECCCCC
GTLVNGNAITEIELHTGDVIEMGEMQMEFLGGQVQ
CEEEECCEEEEEEECCCCEEHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AKDVILTADLGGDLLTAEELAAIPDFAGIRKEIWDKFPGAIARKKYSPGEILMREGENG
CCCEEEEECCCCCEECHHHHHHCCCHHHHHHHHHHHCCCHHHHCCCCCCHHEEECCCCC
TTAFYILSGTIELFINNPVSHVESSRGSSGSWLGGLTKITNYIKGVPAPKSAGGHGRTHI
CEEEEEEEEEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
PIDASVDLPLENPIAEVTSGDLIGELAALAALKQERLKRPKFYPRSATARAKSEVVVLEM
ECCCEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHH
LPNILNNVLYNAASFKERLNKNYRLRALDSHLRTVPVFRNLSADFLEYLRDRVELVDAVP
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
GQVICRQGEIADSFYLIRMGFVKVSQVFPGGELVLTYLSRGSYFGEMGLLPPVFRIRAKG
CCEEECCCCCCCCEEEEHHHHHHHHHCCCCHHHEEEEHHCCCCCCCCCCCCHHHHHEECC
VKPGNLAEVPVSSAEVTVGRAPNSGGAMPIPWDEYISREHATMRVEGRQLRVSRMGSGKN
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCCEEEECCCEEEEEECCCCCC
PITFRMRPVETALLSPGESFVIGETTFEVVEDPLQSGRRTATCTAVDFVQLVRIKAEDFA
CEEEEEECHHHHHCCCCCCEEECCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
QMLERFPEVSTGINEVARARREMDLHLLGRVQTASLSSFLEQELMQGQNLLLLDLDKCTR
HHHHHCCHHHCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECHHCCC
CDECVKACVATHNDGVTRLVRDGLRFENFLVATSCRACMDPLCMTRCPVGSIRRKDSLDI
HHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCEE
VIEDWCIGCGNCAIDCPYGNINVVEVQETKRKQKAEPRPKAVVCDLCAEFAEPNCVRACP
EEECCCCCCCCEEEECCCCCEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEECCC
HDAAIRVEPKTFFARDLAGMQLIVPAGAPAPLPVAPAEPENIETKIYSNVGELLNMLPRL
CCCEEEECCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCE
KIVSGPRSGSVLQLRFPTTTFGRSADNDYRFADDTLMSRAQAVILCEGSRFVLRDLNSTN
EEEECCCCCCEEEEECCCCCCCCCCCCCCEECHHHHHCCCCEEEEECCCEEEEEECCCCC
GTLVNGNAITEIELHTGDVIEMGEMQMEFLGGQVQ
CEEEECCEEEEEEECCCCEEHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]