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The map label for this gene is rpoE [H]

Identifier: 116623292

GI number: 116623292

Start: 5322864

End: 5323529

Strand: Reverse

Name: rpoE [H]

Synonym: Acid_4199

Alternate gene names: 116623292

Gene position: 5323529-5322864 (Counterclockwise)

Preceding gene: 116623303

Following gene: 116623291

Centisome position: 53.42

GC content: 56.01

Gene sequence:

>666_bases
ATGGGAACACTGATCGGCGTCCTCACAGCTCCCGGGTTCGGTAGTAAGGTTGCGGCTAAATCCAGAGTGGACTACCAAAA
CACCCCGGAAGGTGCTTTAGTTCAACGCGCGCAGTCCGGCGACGAAGTGGCATTTCGCGAAATCGTGGAACGCTACCAGG
CCAAGGTGTTTTCCATTATCCACGGCATCGTTCGGCAGCGCAATGACGTGGAAGACATTGCACAGCAAGTATTTGCAAAG
GTTTACCTCTCCATTAAGAGCTTCGATTTCCGCAGCTCGCTCATCACCTGGATCTACAAAATCACGGTCAACGAGTGCTT
CGACTATCTTCGCAAGCGCAAGGTCCGGAAGCTGGTGTATGAAAGCGACATGAGCGAGGACGAGGTTCGGCGCGTGGAGA
ATTCCGAACCGAACGTGGACCGGCAGGTACCGATCGATGCGACGCTGGCGCGTCGCGATTATGTGGTCAAGCTCCTCACC
CGGGTCTCCGAGGAGGAGCGTATGTTGTTGATGATGAAGGAAGTTGAGGGGTTCTCGGTCGAGGAGCTCGCGGCCCAGAC
CGGCATGAATGAGAACACCATCAAAGTAAAGTTATTCCGCGCAAGACAGAAGCTGGTCAAGGCGGCTCAACGCCTGGACC
GGTCTCCCGGCCTGGTCGCCGGTTGA

Upstream 100 bases:

>100_bases
GCGGTGAATTTCGTAATCTATGCGGTGGCAGGGTAGCGTGCTATCGTGCAAAGTGTATCCGCTGAAACCCGGAAAATAAA
CAACACGTCTTTAGGGCTGT

Downstream 100 bases:

>100_bases
ACAGCATGGCAGTACCGGGCTCCCGAAAGCGGGCGGACGAAATTTGTCCAAAGATCTGCAACGGGCTTTTTGGCGTGGTA
ATTTCGTTTGTTAAGGGAAG

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MGTLIGVLTAPGFGSKVAAKSRVDYQNTPEGALVQRAQSGDEVAFREIVERYQAKVFSIIHGIVRQRNDVEDIAQQVFAK
VYLSIKSFDFRSSLITWIYKITVNECFDYLRKRKVRKLVYESDMSEDEVRRVENSEPNVDRQVPIDATLARRDYVVKLLT
RVSEEERMLLMMKEVEGFSVEELAAQTGMNENTIKVKLFRARQKLVKAAQRLDRSPGLVAG

Sequences:

>Translated_221_residues
MGTLIGVLTAPGFGSKVAAKSRVDYQNTPEGALVQRAQSGDEVAFREIVERYQAKVFSIIHGIVRQRNDVEDIAQQVFAK
VYLSIKSFDFRSSLITWIYKITVNECFDYLRKRKVRKLVYESDMSEDEVRRVENSEPNVDRQVPIDATLARRDYVVKLLT
RVSEEERMLLMMKEVEGFSVEELAAQTGMNENTIKVKLFRARQKLVKAAQRLDRSPGLVAG
>Mature_220_residues
GTLIGVLTAPGFGSKVAAKSRVDYQNTPEGALVQRAQSGDEVAFREIVERYQAKVFSIIHGIVRQRNDVEDIAQQVFAKV
YLSIKSFDFRSSLITWIYKITVNECFDYLRKRKVRKLVYESDMSEDEVRRVENSEPNVDRQVPIDATLARRDYVVKLLTR
VSEEERMLLMMKEVEGFSVEELAAQTGMNENTIKVKLFRARQKLVKAAQRLDRSPGLVAG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=184, Percent_Identity=33.1521739130435, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 25253; Mature: 25122

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLIGVLTAPGFGSKVAAKSRVDYQNTPEGALVQRAQSGDEVAFREIVERYQAKVFSII
CCCEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
HGIVRQRNDVEDIAQQVFAKVYLSIKSFDFRSSLITWIYKITVNECFDYLRKRKVRKLVY
HHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESDMSEDEVRRVENSEPNVDRQVPIDATLARRDYVVKLLTRVSEEERMLLMMKEVEGFSV
HCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCH
EELAAQTGMNENTIKVKLFRARQKLVKAAQRLDRSPGLVAG
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GTLIGVLTAPGFGSKVAAKSRVDYQNTPEGALVQRAQSGDEVAFREIVERYQAKVFSII
CCEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
HGIVRQRNDVEDIAQQVFAKVYLSIKSFDFRSSLITWIYKITVNECFDYLRKRKVRKLVY
HHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESDMSEDEVRRVENSEPNVDRQVPIDATLARRDYVVKLLTRVSEEERMLLMMKEVEGFSV
HCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCH
EELAAQTGMNENTIKVKLFRARQKLVKAAQRLDRSPGLVAG
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]