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The map label for this gene is tam [H]

Identifier: 116623278

GI number: 116623278

Start: 5308939

End: 5309700

Strand: Direct

Name: tam [H]

Synonym: Acid_4185

Alternate gene names: 116623278

Gene position: 5308939-5309700 (Clockwise)

Preceding gene: 116623277

Following gene: 116623279

Centisome position: 53.27

GC content: 65.75

Gene sequence:

>762_bases
ATGCCCACCTGGAATGCGTCTCAGTACCTGAAATTCGCGGAAGAGCGCACCCAGCCCTGCCGCGACCTGACCGCGCGCGT
GCCGCTGGCGCATCCAGGCAGCGTCATCGATCTCGGCTGCGGGCCTGGAAACAGTACGCAGGTTCTCGCCGGACGCTGGC
CCGCTGCGCGACTCGCCGGCCTGGATAACTCCGCCGAGATGATCGCACAGGCGCGCGCGTCACGTCCCGATTGGCACTGG
ACAACAGCCAACATCGCCGAGTGGGCCGAAGGCAGCGAACGTTACGACGTGGTTTTCTCGAATGCCGCGCTACAATGGCT
GCCCGATCATCGCAGCCTGTTCCCCAGGCTGATGGACCGGGTCGCTCCCGGCGGCGCACTGGCCGTTCAGATGCCCGGCA
ATGGCGACGCCGCGGCGCAGGTGCTGATGCGGAATGTGGCCGCGCAATGGACCGTGACGCAAGACGTCCGCCAGTGGTTC
ACCCACGATCTCGGCTTCTACTACGACGTGCTCTCGGCGCACGCCGCGCGCGTGGACCTCTGGGCCACGGAGTACATCCA
CATCATGGATGGCGCGGAAGATATCGTCGAGTGGTACAAGGGCACCGGACTCCGACCGTTCCTCGATGCCTTGCCCGCGG
CGGCCGACCGCGACCGTTTCCTCGCCAACTATCTGGACGCGATTCGCGAAGCCTATCCGCCCCACGATGACGGCCGCGTG
CTCCTCCCCTTCCGCCGTATCTTCATGGTCGCTTCGACTTAG

Upstream 100 bases:

>100_bases
AGCCTGAGGCCAGCGTCAGTGCCATCGTCTTTCACCATCCCGATTGCGCCTACTTCACCGCCGACGAAGGCGAATCGGAG
GCGACGGCGTAACAGCGGCG

Downstream 100 bases:

>100_bases
CCTTTTAAGTACGGGCGAATCGCGCTATCCTGTTCCCTAACTGATCCGTAACTGGATCGTCGCTTCAATAGGGAGTCTTC
GTTGCTTAGAACAGCCAGGT

Product: trans-aconitate 2-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MPTWNASQYLKFAEERTQPCRDLTARVPLAHPGSVIDLGCGPGNSTQVLAGRWPAARLAGLDNSAEMIAQARASRPDWHW
TTANIAEWAEGSERYDVVFSNAALQWLPDHRSLFPRLMDRVAPGGALAVQMPGNGDAAAQVLMRNVAAQWTVTQDVRQWF
THDLGFYYDVLSAHAARVDLWATEYIHIMDGAEDIVEWYKGTGLRPFLDALPAAADRDRFLANYLDAIREAYPPHDDGRV
LLPFRRIFMVAST

Sequences:

>Translated_253_residues
MPTWNASQYLKFAEERTQPCRDLTARVPLAHPGSVIDLGCGPGNSTQVLAGRWPAARLAGLDNSAEMIAQARASRPDWHW
TTANIAEWAEGSERYDVVFSNAALQWLPDHRSLFPRLMDRVAPGGALAVQMPGNGDAAAQVLMRNVAAQWTVTQDVRQWF
THDLGFYYDVLSAHAARVDLWATEYIHIMDGAEDIVEWYKGTGLRPFLDALPAAADRDRFLANYLDAIREAYPPHDDGRV
LLPFRRIFMVAST
>Mature_252_residues
PTWNASQYLKFAEERTQPCRDLTARVPLAHPGSVIDLGCGPGNSTQVLAGRWPAARLAGLDNSAEMIAQARASRPDWHWT
TANIAEWAEGSERYDVVFSNAALQWLPDHRSLFPRLMDRVAPGGALAVQMPGNGDAAAQVLMRNVAAQWTVTQDVRQWFT
HDLGFYYDVLSAHAARVDLWATEYIHIMDGAEDIVEWYKGTGLRPFLDALPAAADRDRFLANYLDAIREAYPPHDDGRVL
LPFRRIFMVAST

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate [H]

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family [H]

Homologues:

Organism=Escherichia coli, GI1787798, Length=251, Percent_Identity=45.0199203187251, Blast_Score=214, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR023149 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.144 [H]

Molecular weight: Translated: 28264; Mature: 28133

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTWNASQYLKFAEERTQPCRDLTARVPLAHPGSVIDLGCGPGNSTQVLAGRWPAARLAG
CCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEECCCCHHHHHC
LDNSAEMIAQARASRPDWHWTTANIAEWAEGSERYDVVFSNAALQWLPDHRSLFPRLMDR
CCCHHHHHHHHHCCCCCCCEECCCHHHHHCCCCCEEEEECCCHHEECCCHHHHHHHHHHH
VAPGGALAVQMPGNGDAAAQVLMRNVAAQWTVTQDVRQWFTHDLGFYYDVLSAHAARVDL
HCCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WATEYIHIMDGAEDIVEWYKGTGLRPFLDALPAAADRDRFLANYLDAIREAYPPHDDGRV
HHHHHHEECCCHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
LLPFRRIFMVAST
ECCCHHHHHCCCC
>Mature Secondary Structure 
PTWNASQYLKFAEERTQPCRDLTARVPLAHPGSVIDLGCGPGNSTQVLAGRWPAARLAG
CCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEECCCCHHHHHC
LDNSAEMIAQARASRPDWHWTTANIAEWAEGSERYDVVFSNAALQWLPDHRSLFPRLMDR
CCCHHHHHHHHHCCCCCCCEECCCHHHHHCCCCCEEEEECCCHHEECCCHHHHHHHHHHH
VAPGGALAVQMPGNGDAAAQVLMRNVAAQWTVTQDVRQWFTHDLGFYYDVLSAHAARVDL
HCCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WATEYIHIMDGAEDIVEWYKGTGLRPFLDALPAAADRDRFLANYLDAIREAYPPHDDGRV
HHHHHHEECCCHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
LLPFRRIFMVAST
ECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA