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The map label for this gene is rhgT [H]

Identifier: 116623146

GI number: 116623146

Start: 5141628

End: 5142836

Strand: Reverse

Name: rhgT [H]

Synonym: Acid_4052

Alternate gene names: 116623146

Gene position: 5142836-5141628 (Counterclockwise)

Preceding gene: 116623148

Following gene: 116623142

Centisome position: 51.61

GC content: 61.29

Gene sequence:

>1209_bases
ATGAAATGTCTGATCGCCTTTTTCCTGCTAGCTTCGGCCTGGGGACAGAAGTTTGATTTGAGCAGCGGCAAGCCGGAGAT
ATATTCCGCCGAGCGCGGCTATGGCTTCGAAGAGGGCGCCAAAGTTCGCTCCGGCGCAGGCTCCATCACGAGCGACCAGC
CGCCCTTCTATTTCTCCGCCAAAGTGCCGGTGGAAGGGAACTATCGCGTCACCGTCACGCTGGGCGATCGCGAGGCTCCC
ACCGTGACCACCGTCAAGGCCGAGCTGCGGCGCCTGATGGTGGAAAAGGTGCGGACCGAGCCCGGTGAATTCGTCAGGCG
CACCTTCATCGTCAACACCCGCACCCCACGCATCGACGCCAGCACCGAAGTCCGCCTGAAGGATCGCGAGAAGACCTCCG
AAGCCTGGGCGTGGGATGACCGCATCACGCTCGAGTTCGCCAATCAGCACCCCGCCGTGAGCGCGATTGAGATCGAAAAG
GCCGATGTCCCCACGCTCTACATCGCCGGCGATTCCACTTCGACCGATCAGGGTCGCGAGCCCTTCAACAGTTGGGGACA
GATGCTGCCGCGCTTCTTCACGCCGCGCATCGCGGTGGCGAACCACGGCGAAAGCGGCGAATCCCTGCGCAGTTTCATCG
GCGAGCGCCGCCTCGCTAAACTTTCCACCCTCATGAAATCCGGCGACTGGCTGCTCATCCAGATGGGTCACAACGACCAG
AAAGAGAAAGGCGAGGGCGTCGGCGCCTTCACCACTTATAAGGAGTCGCTCAAGCAATTGATCGCCGAGGCACGCCGGCT
GGGCGTGACCCCGATCCTGGTGACGCCGATGCATCGCATGACCTTCGACTCCGCCGGCAAGATCACCAACAGCCTGGGAG
ACTATCCCGAGGCAGTGCGCCAGACCGGCGCCGAAGAGAAGGTCGAAGTGATCGATCTCAACGCCGTGAGCAAGCTTTTC
TATGAGGCGCTCGGTCTCAGGGAGGCGGTGAAGGCTTTCGCCGATGGCGACACCACCCACCACAACAATTACGGCAGCTA
TGAATTGGCCCGTTGCATCGTTGAAGCCATCCGCGAGAGCAAGTCAGCCTTGGTGAAGTATCTCGTGGAGGATGTCTCAC
CGTTCGACCCGTCGCACCCCGACCCCCTGTCGAAATTCGATATCCCCGCCGACCCCCGAGGTACGGTCCAGAAGCCCTAC
GGAAATTAG

Upstream 100 bases:

>100_bases
CGTAGCGGGGTGTGCTCTGCGCGAACGCCGCGCAGGCGAACCAGAAAATCGCGAGCAACCTCATCGCCACCAAGGTACCA
AACCAAGGTACCAACAAATC

Downstream 100 bases:

>100_bases
TTTTGTTCTTCTTGGCGTTACTTTGCGTCTCTGCGAGAAATCCTCCCTCTCTCGCAAAGCCGCAAAGCCGCAAAGTAACG
CCAAGATAACTCCTACCGCG

Product: GDSL family lipase

Products: NA

Alternate protein names: RGAE [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKCLIAFFLLASAWGQKFDLSSGKPEIYSAERGYGFEEGAKVRSGAGSITSDQPPFYFSAKVPVEGNYRVTVTLGDREAP
TVTTVKAELRRLMVEKVRTEPGEFVRRTFIVNTRTPRIDASTEVRLKDREKTSEAWAWDDRITLEFANQHPAVSAIEIEK
ADVPTLYIAGDSTSTDQGREPFNSWGQMLPRFFTPRIAVANHGESGESLRSFIGERRLAKLSTLMKSGDWLLIQMGHNDQ
KEKGEGVGAFTTYKESLKQLIAEARRLGVTPILVTPMHRMTFDSAGKITNSLGDYPEAVRQTGAEEKVEVIDLNAVSKLF
YEALGLREAVKAFADGDTTHHNNYGSYELARCIVEAIRESKSALVKYLVEDVSPFDPSHPDPLSKFDIPADPRGTVQKPY
GN

Sequences:

>Translated_402_residues
MKCLIAFFLLASAWGQKFDLSSGKPEIYSAERGYGFEEGAKVRSGAGSITSDQPPFYFSAKVPVEGNYRVTVTLGDREAP
TVTTVKAELRRLMVEKVRTEPGEFVRRTFIVNTRTPRIDASTEVRLKDREKTSEAWAWDDRITLEFANQHPAVSAIEIEK
ADVPTLYIAGDSTSTDQGREPFNSWGQMLPRFFTPRIAVANHGESGESLRSFIGERRLAKLSTLMKSGDWLLIQMGHNDQ
KEKGEGVGAFTTYKESLKQLIAEARRLGVTPILVTPMHRMTFDSAGKITNSLGDYPEAVRQTGAEEKVEVIDLNAVSKLF
YEALGLREAVKAFADGDTTHHNNYGSYELARCIVEAIRESKSALVKYLVEDVSPFDPSHPDPLSKFDIPADPRGTVQKPY
GN
>Mature_402_residues
MKCLIAFFLLASAWGQKFDLSSGKPEIYSAERGYGFEEGAKVRSGAGSITSDQPPFYFSAKVPVEGNYRVTVTLGDREAP
TVTTVKAELRRLMVEKVRTEPGEFVRRTFIVNTRTPRIDASTEVRLKDREKTSEAWAWDDRITLEFANQHPAVSAIEIEK
ADVPTLYIAGDSTSTDQGREPFNSWGQMLPRFFTPRIAVANHGESGESLRSFIGERRLAKLSTLMKSGDWLLIQMGHNDQ
KEKGEGVGAFTTYKESLKQLIAEARRLGVTPILVTPMHRMTFDSAGKITNSLGDYPEAVRQTGAEEKVEVIDLNAVSKLF
YEALGLREAVKAFADGDTTHHNNYGSYELARCIVEAIRESKSALVKYLVEDVSPFDPSHPDPLSKFDIPADPRGTVQKPY
GN

Specific function: May play role in the degradation of type I rhamnogalacturonan derived from plant cell walls. This enzyme has a broad substrate specificity, and shows strong preference for glucose pentaacetate, beta-naphthylacetate, and p-nitrophenyl acetate (pNPA). Also

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: NA

Molecular weight: Translated: 44709; Mature: 44709

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCLIAFFLLASAWGQKFDLSSGKPEIYSAERGYGFEEGAKVRSGAGSITSDQPPFYFSA
CHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
KVPVEGNYRVTVTLGDREAPTVTTVKAELRRLMVEKVRTEPGEFVRRTFIVNTRTPRIDA
ECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCC
STEVRLKDREKTSEAWAWDDRITLEFANQHPAVSAIEIEKADVPTLYIAGDSTSTDQGRE
CCCEEECCCCCCCCCEECCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCC
PFNSWGQMLPRFFTPRIAVANHGESGESLRSFIGERRLAKLSTLMKSGDWLLIQMGHNDQ
HHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
KEKGEGVGAFTTYKESLKQLIAEARRLGVTPILVTPMHRMTFDSAGKITNSLGDYPEAVR
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCHHHHHHHHCCHHHHHH
QTGAEEKVEVIDLNAVSKLFYEALGLREAVKAFADGDTTHHNNYGSYELARCIVEAIRES
HCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
KSALVKYLVEDVSPFDPSHPDPLSKFDIPADPRGTVQKPYGN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKCLIAFFLLASAWGQKFDLSSGKPEIYSAERGYGFEEGAKVRSGAGSITSDQPPFYFSA
CHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
KVPVEGNYRVTVTLGDREAPTVTTVKAELRRLMVEKVRTEPGEFVRRTFIVNTRTPRIDA
ECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCC
STEVRLKDREKTSEAWAWDDRITLEFANQHPAVSAIEIEKADVPTLYIAGDSTSTDQGRE
CCCEEECCCCCCCCCEECCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCC
PFNSWGQMLPRFFTPRIAVANHGESGESLRSFIGERRLAKLSTLMKSGDWLLIQMGHNDQ
HHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
KEKGEGVGAFTTYKESLKQLIAEARRLGVTPILVTPMHRMTFDSAGKITNSLGDYPEAVR
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCHHHHHHHHCCHHHHHH
QTGAEEKVEVIDLNAVSKLFYEALGLREAVKAFADGDTTHHNNYGSYELARCIVEAIRES
HCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
KSALVKYLVEDVSPFDPSHPDPLSKFDIPADPRGTVQKPYGN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]