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The map label for this gene is amaB [H]

Identifier: 116623145

GI number: 116623145

Start: 5140192

End: 5141535

Strand: Direct

Name: amaB [H]

Synonym: Acid_4051

Alternate gene names: 116623145

Gene position: 5140192-5141535 (Clockwise)

Preceding gene: 116623144

Following gene: 116623147

Centisome position: 51.58

GC content: 66.89

Gene sequence:

>1344_bases
TTGCGCTCCTTCACGGTCGCGGCTCGTTGTACACTGGCACGAATGAACCGGCGCACTTTTTTGGGCACGCTTGCGGTTAC
GGCGCTGGCGCAATCGCCCGCACCCCGCGTGAATGCGGGCAGACTTCGCGAACACCTCGAGGCGCTCAGCGTCTTTGGAC
GTCCCGAGGGCGGGACGTTCGCCAGCGGAGTGAGCCGCTACGCGTATTCGGACGCCGACGTCGCGGGGCGCGCGTACGTC
ATGAAACTGATGCAAGCCGCGGGATTGAAGCCGCGCATCGATGCGGCGGGAAATATCTACGGGCTACGTCCGGGCAGCGA
CGCGGCGTTGCCGCCGATGCTCTTCGGGTCGCATATCGATTCGGTGCCTAACGGCGGCAACTTCGACGGCGATCTCGGCT
CGCTGGCCGCGATCGAGGTGGTGCAGACGCTGAATGAGGCGCACGTCGCCACGCGGCGCGGCCTGGAGATCGTCATCTGG
CAGAACGAGGAGGGGTACGCATTCAACAACGGGCTGGTGGGCAGCCGCGCCGCCGCCGGGCATCTGGACCGGGGCGAATT
GGACGCGGTGTGGAACGGCGTGAAAAAGTCGGACGCGATCCGCAAGATCGGCGGGAATCCCGAGCGGATCGCGGAGGCAA
AGCGCGGGCCGAACAGCTTCGCGTGCTACCTGGAACTTCACATCGAGCAGGGCGGAACGCTGGAGCGCGCCGGAATTCCG
ATCGGCGTGGTGGAGGGCATCGTATCGATCGACCGCTACGACGTGGAAGTCCGCGGATTTGCCAACCATGCGGGCACCAC
ACCCATGCCGGACCGGCAGGACGCGCTGGTGGCGGCGTCGATGCTGACGGTCGCGGTGAACGACGAGGTGAGGGCGATGC
CGGGCCGGCAGGTAGGGACGGTGGGGCAGTTGAACGTGAGCCCGAACGTGCCGAACATCGTGCCGGGGTCGGTGAAGATG
GTGGTGGAGCTGCGCGATCTCTCGGAAAAGAGAGTCGCCAGTCTCGGCGCGAGGATAGGCTCGCGGGCGCGGGAGATCGC
GGCGCAGACCAGGACCGAGATTACGATGAAGCTGGCGGCGCACCACGATGCGGCGTTGGCCACACCGGAAGTGCAACGCT
CGATCGAAGCGGCCTGCGCGAAGCTGGGGCTGAAGACCCAGCGGCAGCCGAGCGGCGCAGGCCACGACGCGCAGATGATG
GCGCTGCTGGGGCCGATGGGGATGATCTTCGTGCCGAGCGTCAAAGGGATCAGCCATTCTCCGGCGGAACTCACGAGCTG
GGAAGATTGCGCGCGCGGGGCCGACGTGCTGCTGGCGACGGTGCTGGACTGGGCGTCGCGGTAG

Upstream 100 bases:

>100_bases
TGCGGATGTTTGCGGCCTGCGAGCAGACAAAGGCACAGTAGTACAGGGAAATCGTGAGATCGGTGTTTGGTTTGGGGCGA
TGCCGGAGATTAAGGGCACT

Downstream 100 bases:

>100_bases
GAGTTATCTTGGCGTTACTTTGCGGCTTTGCGGCTTTGCGAGAGAGGGAGGATTTCTCGCAGAGACGCAAAGTAACGCCA
AGAAGAACAAAACTAATTTC

Product: amidase

Products: NA

Alternate protein names: L-carbamoylase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MRSFTVAARCTLARMNRRTFLGTLAVTALAQSPAPRVNAGRLREHLEALSVFGRPEGGTFASGVSRYAYSDADVAGRAYV
MKLMQAAGLKPRIDAAGNIYGLRPGSDAALPPMLFGSHIDSVPNGGNFDGDLGSLAAIEVVQTLNEAHVATRRGLEIVIW
QNEEGYAFNNGLVGSRAAAGHLDRGELDAVWNGVKKSDAIRKIGGNPERIAEAKRGPNSFACYLELHIEQGGTLERAGIP
IGVVEGIVSIDRYDVEVRGFANHAGTTPMPDRQDALVAASMLTVAVNDEVRAMPGRQVGTVGQLNVSPNVPNIVPGSVKM
VVELRDLSEKRVASLGARIGSRAREIAAQTRTEITMKLAAHHDAALATPEVQRSIEAACAKLGLKTQRQPSGAGHDAQMM
ALLGPMGMIFVPSVKGISHSPAELTSWEDCARGADVLLATVLDWASR

Sequences:

>Translated_447_residues
MRSFTVAARCTLARMNRRTFLGTLAVTALAQSPAPRVNAGRLREHLEALSVFGRPEGGTFASGVSRYAYSDADVAGRAYV
MKLMQAAGLKPRIDAAGNIYGLRPGSDAALPPMLFGSHIDSVPNGGNFDGDLGSLAAIEVVQTLNEAHVATRRGLEIVIW
QNEEGYAFNNGLVGSRAAAGHLDRGELDAVWNGVKKSDAIRKIGGNPERIAEAKRGPNSFACYLELHIEQGGTLERAGIP
IGVVEGIVSIDRYDVEVRGFANHAGTTPMPDRQDALVAASMLTVAVNDEVRAMPGRQVGTVGQLNVSPNVPNIVPGSVKM
VVELRDLSEKRVASLGARIGSRAREIAAQTRTEITMKLAAHHDAALATPEVQRSIEAACAKLGLKTQRQPSGAGHDAQMM
ALLGPMGMIFVPSVKGISHSPAELTSWEDCARGADVLLATVLDWASR
>Mature_447_residues
MRSFTVAARCTLARMNRRTFLGTLAVTALAQSPAPRVNAGRLREHLEALSVFGRPEGGTFASGVSRYAYSDADVAGRAYV
MKLMQAAGLKPRIDAAGNIYGLRPGSDAALPPMLFGSHIDSVPNGGNFDGDLGSLAAIEVVQTLNEAHVATRRGLEIVIW
QNEEGYAFNNGLVGSRAAAGHLDRGELDAVWNGVKKSDAIRKIGGNPERIAEAKRGPNSFACYLELHIEQGGTLERAGIP
IGVVEGIVSIDRYDVEVRGFANHAGTTPMPDRQDALVAASMLTVAVNDEVRAMPGRQVGTVGQLNVSPNVPNIVPGSVKM
VVELRDLSEKRVASLGARIGSRAREIAAQTRTEITMKLAAHHDAALATPEVQRSIEAACAKLGLKTQRQPSGAGHDAQMM
ALLGPMGMIFVPSVKGISHSPAELTSWEDCARGADVLLATVLDWASR

Specific function: Involved In The Anaerobic Utilization Of Allantoin. Converts Allantoate To (S)-Ureidoglycolate And Ammonia. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1786726, Length=399, Percent_Identity=33.8345864661654, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010158
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.87 [H]

Molecular weight: Translated: 47394; Mature: 47394

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFTVAARCTLARMNRRTFLGTLAVTALAQSPAPRVNAGRLREHLEALSVFGRPEGGTF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCH
ASGVSRYAYSDADVAGRAYVMKLMQAAGLKPRIDAAGNIYGLRPGSDAALPPMLFGSHID
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHC
SVPNGGNFDGDLGSLAAIEVVQTLNEAHVATRRGLEIVIWQNEEGYAFNNGLVGSRAAAG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCHHCC
HLDRGELDAVWNGVKKSDAIRKIGGNPERIAEAKRGPNSFACYLELHIEQGGTLERAGIP
CCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEEECCCCEEECCCCC
IGVVEGIVSIDRYDVEVRGFANHAGTTPMPDRQDALVAASMLTVAVNDEVRAMPGRQVGT
HHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHEEEEECCCHHCCCCCCCCC
VGQLNVSPNVPNIVPGSVKMVVELRDLSEKRVASLGARIGSRAREIAAQTRTEITMKLAA
EEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHH
HHDAALATPEVQRSIEAACAKLGLKTQRQPSGAGHDAQMMALLGPMGMIFVPSVKGISHS
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCC
PAELTSWEDCARGADVLLATVLDWASR
CCCCCCHHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSFTVAARCTLARMNRRTFLGTLAVTALAQSPAPRVNAGRLREHLEALSVFGRPEGGTF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCH
ASGVSRYAYSDADVAGRAYVMKLMQAAGLKPRIDAAGNIYGLRPGSDAALPPMLFGSHID
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHC
SVPNGGNFDGDLGSLAAIEVVQTLNEAHVATRRGLEIVIWQNEEGYAFNNGLVGSRAAAG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCHHCC
HLDRGELDAVWNGVKKSDAIRKIGGNPERIAEAKRGPNSFACYLELHIEQGGTLERAGIP
CCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEEECCCCEEECCCCC
IGVVEGIVSIDRYDVEVRGFANHAGTTPMPDRQDALVAASMLTVAVNDEVRAMPGRQVGT
HHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHEEEEECCCHHCCCCCCCCC
VGQLNVSPNVPNIVPGSVKMVVELRDLSEKRVASLGARIGSRAREIAAQTRTEITMKLAA
EEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHH
HHDAALATPEVQRSIEAACAKLGLKTQRQPSGAGHDAQMMALLGPMGMIFVPSVKGISHS
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCC
PAELTSWEDCARGADVLLATVLDWASR
CCCCCCHHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9023955; 8285691 [H]