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The map label for this gene is ogt [H]

Identifier: 116623098

GI number: 116623098

Start: 5070496

End: 5071020

Strand: Direct

Name: ogt [H]

Synonym: Acid_4002

Alternate gene names: 116623098

Gene position: 5070496-5071020 (Clockwise)

Preceding gene: 116623097

Following gene: 116623100

Centisome position: 50.88

GC content: 61.9

Gene sequence:

>525_bases
ATGCGTGACGTACTTCAGTTATCGATCGACCGGGTGGAAACGCCGATCGGAGAATTGCTGCTGGTGGCGGACCACGACGG
GAGTCTGCGCGCGGTCGATTGGGCCGACTATGAAGCGCGCATGCTGCGCCTGCTGGGAATTCATTACAGGAAGAACGGGT
TCCGGCTGCAGCCGGCTCGCAATCCGCATGGCTTGCGCGACGCGATCACGAGCTACTTTGGAGGCGAACTCGCCGCGATC
GACGATCTTCCGGTAAGGACGGCCGGCACAGATTTTCAGCGGAGCGTGTGGAGTGCGCTAAGGAAGATTCCATGCGGTGC
TCCGACATCCTACGGCAAGCTCGCGGAGGCGATCGACCGGCCGACAGCGGTAAGAGCCGTGGGACTGGCGAATGGGTCCA
ACCCTATTGGGATCGTGGTGCCGTGTCACCGGGTCATTGGGGCGAAGGGATCGCTCACAGGGTACGGCGGGGGATTGGAG
CGCAAGCGGTGGCTGCTGGAGCATGAGGGAATGCACGGTATTTGA

Upstream 100 bases:

>100_bases
GGCGCCAAACCTTCGGTGGATGACCTCCTGCGGCGAGCGGAATCCTGGCGGCCCTGGCGCGCGTATGCGGCACAGCATCT
CTGGGCGGCGGATCGAACTT

Downstream 100 bases:

>100_bases
GGTCTTCTTTGCGTGACTTTGCGGCTTTGCGAGAGAGTGTAAATTTCTCGCAGAGCCGCAGAGCCGCAGAGTCACGCAGA
GGAAAACCGAAATCGGAATT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MRDVLQLSIDRVETPIGELLLVADHDGSLRAVDWADYEARMLRLLGIHYRKNGFRLQPARNPHGLRDAITSYFGGELAAI
DDLPVRTAGTDFQRSVWSALRKIPCGAPTSYGKLAEAIDRPTAVRAVGLANGSNPIGIVVPCHRVIGAKGSLTGYGGGLE
RKRWLLEHEGMHGI

Sequences:

>Translated_174_residues
MRDVLQLSIDRVETPIGELLLVADHDGSLRAVDWADYEARMLRLLGIHYRKNGFRLQPARNPHGLRDAITSYFGGELAAI
DDLPVRTAGTDFQRSVWSALRKIPCGAPTSYGKLAEAIDRPTAVRAVGLANGSNPIGIVVPCHRVIGAKGSLTGYGGGLE
RKRWLLEHEGMHGI
>Mature_174_residues
MRDVLQLSIDRVETPIGELLLVADHDGSLRAVDWADYEARMLRLLGIHYRKNGFRLQPARNPHGLRDAITSYFGGELAAI
DDLPVRTAGTDFQRSVWSALRKIPCGAPTSYGKLAEAIDRPTAVRAVGLANGSNPIGIVVPCHRVIGAKGSLTGYGGGLE
RKRWLLEHEGMHGI

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=109, Percent_Identity=39.4495412844037, Blast_Score=74, Evalue=6e-14,
Organism=Escherichia coli, GI1787596, Length=167, Percent_Identity=56.2874251497006, Blast_Score=200, Evalue=4e-53,
Organism=Escherichia coli, GI1788542, Length=88, Percent_Identity=51.1363636363636, Blast_Score=97, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=86, Percent_Identity=47.6744186046512, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=86, Percent_Identity=47.6744186046512, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=86, Percent_Identity=44.1860465116279, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137554, Length=87, Percent_Identity=44.8275862068966, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18976; Mature: 18976

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDVLQLSIDRVETPIGELLLVADHDGSLRAVDWADYEARMLRLLGIHYRKNGFRLQPAR
CCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEECCCC
NPHGLRDAITSYFGGELAAIDDLPVRTAGTDFQRSVWSALRKIPCGAPTSYGKLAEAIDR
CCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
PTAVRAVGLANGSNPIGIVVPCHRVIGAKGSLTGYGGGLERKRWLLEHEGMHGI
CCCEEEEEECCCCCCEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRDVLQLSIDRVETPIGELLLVADHDGSLRAVDWADYEARMLRLLGIHYRKNGFRLQPAR
CCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEECCCC
NPHGLRDAITSYFGGELAAIDDLPVRTAGTDFQRSVWSALRKIPCGAPTSYGKLAEAIDR
CCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
PTAVRAVGLANGSNPIGIVVPCHRVIGAKGSLTGYGGGLERKRWLLEHEGMHGI
CCCEEEEEECCCCCCEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2825131; 9097039; 9278503; 1520330 [H]