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The map label for this gene is ada [H]

Identifier: 116623097

GI number: 116623097

Start: 5069034

End: 5070503

Strand: Direct

Name: ada [H]

Synonym: Acid_4001

Alternate gene names: 116623097

Gene position: 5069034-5070503 (Clockwise)

Preceding gene: 116623096

Following gene: 116623098

Centisome position: 50.87

GC content: 67.35

Gene sequence:

>1470_bases
ATGGAGCTGCCTGACCGGGAGACTTGTTATCGCGCGTTGCAGAGCCGCGATGCGCGCTTCGACGGTTTGCTGTTCGTCGG
CATCACGTCCACCGGCATCTACTGCCGGCCGGTCTGCCCGGCGCGCACCGCGAAGCCCGAGAACTGCCGTTTCTTCGGTT
CGGCGGCGGCAGCCCAGGAAGCGGGCTTTCGACCGTGCCTGCGCTGCCGCCCGGAAACGGCGCCCGACACGGCGTCGTGG
CGCGGAACGTCGAATACGGTGTCACGCGCGCTAGCCCTGATCGCAGATGGCGCACTGGACGCCGAGAATGCCGGCGTGGA
ACTGCTGGCGGAGCGGCTGGGCATCGGCGAACGCCATTTGCGGCGGCTGTTCGTTCAACACCTGGGCGCCTCGCCGGTGG
CGGTGGCGCAGACCCGGCGCGTCCTTTTCGCCAAGCAATTGATCCACGAAACACAGATGCCGATGACGGAAGTCGCGCTG
GCAGCGGGGTTCGGCAGCATCCGGCGATTCAACGAGATATTTCGCAAGCTGTTTCATCGTCCTCCGAGCGCGCTGCGACG
GAAATCTTCCACTAGCCAGGAACCCGGCGTGACGCTGCGACTGCGCTACCGGCCGCCCTACGACTGGGAGGCGATGCTCG
CGTTCCTGCGAGCGCGCGCCATCCCCGGCGTCGAGGTGGTGGAGAACGGAAGTTATCTCCGCACGGTAGGGATGGATGGC
GTGACGGGGAGCGTCGAGGTGCGCCATTTGGCGGAGCGGCAAAGCCTGGGCGTCACGATCTGTTTTCCGCGTGTGCAATC
GCTGCCGGCGATCATCGCTCGCGTGCGGCGCGTGTTCGACCTGGGCGCGGACATCGAGACCATCGACGCCCACCTGTCGC
TTGACCCGCTGCTGGCGCCGTTTGTGGCGCAGCGTCCGGGGCTGCGCGCTCCAGGCGGATGGGATGGCTTCGAACTGGCG
GTGAGGGCGATTCTGGGCCAGCAGGTAACGGTCGCCGCGGCCCGGCGGCTGGCGGGACAACTGGTCGCGCACCATGGCGA
ACCGGTGTCGAGTGACCTGGTGAGCGACGCGAGGTTAACGCACGTATTTCCAGAGGCCCAGCGGATTGCGCGCGCGGACT
CCATTAATATGGGCATGCCGCGGGCGCGAATTTCCGCGCTGAAGGAACTTGCGGAGGCCGCGGAGGCGGATCCCAATCTG
TTTCGCCCGTTCGGGACGATCGGGGAGGCCATCGCCAGGTTGCGGACCATCCGGGGCGTGGGCGAGTGGACCGCGCAATA
CATCGCCCTGCGCGCATTGCGTGAGACCGACGCGTTCCCGGCGGCCGATATCGGGCTGCTGCGCGGCGCGGAGGTGGTGG
ACGGCGCCAAACCTTCGGTGGATGACCTCCTGCGGCGAGCGGAATCCTGGCGGCCCTGGCGCGCGTATGCGGCACAGCAT
CTCTGGGCGGCGGATCGAACTTATGCGTGA

Upstream 100 bases:

>100_bases
CGCACCGCAGCGCGACAGGGGTCCGGTCAATGCCGCGGTGATGTTTATCCATGTCCGAAAACCGCTAGTGTGAGGGTTTC
TTCGCTGGGACAATGGACTC

Downstream 100 bases:

>100_bases
CGTACTTCAGTTATCGATCGACCGGGTGGAAACGCCGATCGGAGAATTGCTGCTGGTGGCGGACCACGACGGGAGTCTGC
GCGCGGTCGATTGGGCCGAC

Product: DNA-3-methyladenine glycosylase II/transcriptional regulator Ada/DNA-O6-methylguanine--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MELPDRETCYRALQSRDARFDGLLFVGITSTGIYCRPVCPARTAKPENCRFFGSAAAAQEAGFRPCLRCRPETAPDTASW
RGTSNTVSRALALIADGALDAENAGVELLAERLGIGERHLRRLFVQHLGASPVAVAQTRRVLFAKQLIHETQMPMTEVAL
AAGFGSIRRFNEIFRKLFHRPPSALRRKSSTSQEPGVTLRLRYRPPYDWEAMLAFLRARAIPGVEVVENGSYLRTVGMDG
VTGSVEVRHLAERQSLGVTICFPRVQSLPAIIARVRRVFDLGADIETIDAHLSLDPLLAPFVAQRPGLRAPGGWDGFELA
VRAILGQQVTVAAARRLAGQLVAHHGEPVSSDLVSDARLTHVFPEAQRIARADSINMGMPRARISALKELAEAAEADPNL
FRPFGTIGEAIARLRTIRGVGEWTAQYIALRALRETDAFPAADIGLLRGAEVVDGAKPSVDDLLRRAESWRPWRAYAAQH
LWAADRTYA

Sequences:

>Translated_489_residues
MELPDRETCYRALQSRDARFDGLLFVGITSTGIYCRPVCPARTAKPENCRFFGSAAAAQEAGFRPCLRCRPETAPDTASW
RGTSNTVSRALALIADGALDAENAGVELLAERLGIGERHLRRLFVQHLGASPVAVAQTRRVLFAKQLIHETQMPMTEVAL
AAGFGSIRRFNEIFRKLFHRPPSALRRKSSTSQEPGVTLRLRYRPPYDWEAMLAFLRARAIPGVEVVENGSYLRTVGMDG
VTGSVEVRHLAERQSLGVTICFPRVQSLPAIIARVRRVFDLGADIETIDAHLSLDPLLAPFVAQRPGLRAPGGWDGFELA
VRAILGQQVTVAAARRLAGQLVAHHGEPVSSDLVSDARLTHVFPEAQRIARADSINMGMPRARISALKELAEAAEADPNL
FRPFGTIGEAIARLRTIRGVGEWTAQYIALRALRETDAFPAADIGLLRGAEVVDGAKPSVDDLLRRAESWRPWRAYAAQH
LWAADRTYA
>Mature_489_residues
MELPDRETCYRALQSRDARFDGLLFVGITSTGIYCRPVCPARTAKPENCRFFGSAAAAQEAGFRPCLRCRPETAPDTASW
RGTSNTVSRALALIADGALDAENAGVELLAERLGIGERHLRRLFVQHLGASPVAVAQTRRVLFAKQLIHETQMPMTEVAL
AAGFGSIRRFNEIFRKLFHRPPSALRRKSSTSQEPGVTLRLRYRPPYDWEAMLAFLRARAIPGVEVVENGSYLRTVGMDG
VTGSVEVRHLAERQSLGVTICFPRVQSLPAIIARVRRVFDLGADIETIDAHLSLDPLLAPFVAQRPGLRAPGGWDGFELA
VRAILGQQVTVAAARRLAGQLVAHHGEPVSSDLVSDARLTHVFPEAQRIARADSINMGMPRARISALKELAEAAEADPNL
FRPFGTIGEAIARLRTIRGVGEWTAQYIALRALRETDAFPAADIGLLRGAEVVDGAKPSVDDLLRRAESWRPWRAYAAQH
LWAADRTYA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=286, Percent_Identity=35.6643356643357, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788542, Length=156, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 53703; Mature: 53703

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELPDRETCYRALQSRDARFDGLLFVGITSTGIYCRPVCPARTAKPENCRFFGSAAAAQE
CCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCEEECCHHHHHH
AGFRPCLRCRPETAPDTASWRGTSNTVSRALALIADGALDAENAGVELLAERLGIGERHL
CCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHH
RRLFVQHLGASPVAVAQTRRVLFAKQLIHETQMPMTEVALAAGFGSIRRFNEIFRKLFHR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PPSALRRKSSTSQEPGVTLRLRYRPPYDWEAMLAFLRARAIPGVEVVENGSYLRTVGMDG
CCHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCEEEEECCCC
VTGSVEVRHLAERQSLGVTICFPRVQSLPAIIARVRRVFDLGADIETIDAHLSLDPLLAP
CCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHH
FVAQRPGLRAPGGWDGFELAVRAILGQQVTVAAARRLAGQLVAHHGEPVSSDLVSDARLT
HHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
HVFPEAQRIARADSINMGMPRARISALKELAEAAEADPNLFRPFGTIGEAIARLRTIRGV
HHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCHHHHHHHHHHHHHHHHHH
GEWTAQYIALRALRETDAFPAADIGLLRGAEVVDGAKPSVDDLLRRAESWRPWRAYAAQH
HHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCCCCCCHHHHHHHHHCCCHHHHHHHHH
LWAADRTYA
HHHCCCCCC
>Mature Secondary Structure
MELPDRETCYRALQSRDARFDGLLFVGITSTGIYCRPVCPARTAKPENCRFFGSAAAAQE
CCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCEEECCHHHHHH
AGFRPCLRCRPETAPDTASWRGTSNTVSRALALIADGALDAENAGVELLAERLGIGERHL
CCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHH
RRLFVQHLGASPVAVAQTRRVLFAKQLIHETQMPMTEVALAAGFGSIRRFNEIFRKLFHR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PPSALRRKSSTSQEPGVTLRLRYRPPYDWEAMLAFLRARAIPGVEVVENGSYLRTVGMDG
CCHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCEEEEECCCC
VTGSVEVRHLAERQSLGVTICFPRVQSLPAIIARVRRVFDLGADIETIDAHLSLDPLLAP
CCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHH
FVAQRPGLRAPGGWDGFELAVRAILGQQVTVAAARRLAGQLVAHHGEPVSSDLVSDARLT
HHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
HVFPEAQRIARADSINMGMPRARISALKELAEAAEADPNLFRPFGTIGEAIARLRTIRGV
HHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCHHHHHHHHHHHHHHHHHH
GEWTAQYIALRALRETDAFPAADIGLLRGAEVVDGAKPSVDDLLRRAESWRPWRAYAAQH
HHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCCCCCCHHHHHHHHHCCCHHHHHHHHH
LWAADRTYA
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]