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The map label for this gene is hisF

Identifier: 116622948

GI number: 116622948

Start: 4885471

End: 4886235

Strand: Reverse

Name: hisF

Synonym: Acid_3850

Alternate gene names: 116622948

Gene position: 4886235-4885471 (Counterclockwise)

Preceding gene: 116622949

Following gene: 116622947

Centisome position: 49.03

GC content: 67.19

Gene sequence:

>765_bases
ATGCTAGCCAAGCGCATCATCCCGTGCCTCGACGTTACCGGCGGCCGCGTGGTCAAAGGCGTTAACTTCGTCAACCTACG
CGATGCTGGAGATCCCGTGGAGCTCGCCGATCGCTACAACCTCGACGGCGCCGACGAGCTCGTCTTCCTCGACATCACTG
CCTCCAGCGACGCGCGCGACATCATGGCCGATGTGGTCGCCCGCACCGCGCGCAAGGTCTTCATCCCGCTGGCCGTCGGC
GGCGGCATCCGCAGCATCGCCGACGCACGCCACATCCTGCTCTCCGGCGCCGACAAAGTCTCTGTCAACACCGCCGCCGT
GCGCCGTCCCGAGTTGATCACGGAATTGAGCCGTGAATTAGGCGCGCAGGCCGTCGTGCTGGCCATTGACGCGCGCCGCC
ACGGTCCCGGCGGCTGGCACGTGTACACCCGAGGCGGCCGCGACGATGAAGGCATGGACGCCGTCGCCTGGGCTGCACGC
GGCGAAGCGCTCGGCGCCGGCGAAGTCCTGCTCACCTCCATGGATACCGATGGCGTGCAGGACGGTTTCGATTGCGCCCT
CACGATGGCCGTCTCCCGCGCCACGCACATTCCGGTGATCGCGAGCGGCGGGGCGGGGAAGCCCGAGCACTTCGTTCGCG
TGCTCACCGACGGCTGCGCCGATGCCGCGCTCGCCGCGTCGATATTCCACTACGGAACCTATACCGTGAATCAACTCAAG
GAAGCGCTCGATAAGCGCGGCATCCCCGTGCGGGTGACGGCATGA

Upstream 100 bases:

>100_bases
AGCACGGTCGGCCTCCAGATCGTGAAAGGCTTCCTCGAATGTTAATGTGTTCGCCGGCGGGCGCCTTCAATCCGAGCCGC
GAGCGTCAGGGAGCGTCCTC

Downstream 100 bases:

>100_bases
TCGTCCCCTGCATCGACCTGATGGATGGCAAGGTGGTCCAGCTCATCCAGGGCCGCGAGAAGGCGCTCGAAGGCGATAGC
CCCGACGAAATGCTGCGCAG

Product: imidazole glycerol phosphate synthase subunit hisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLAKRIIPCLDVTGGRVVKGVNFVNLRDAGDPVELADRYNLDGADELVFLDITASSDARDIMADVVARTARKVFIPLAVG
GGIRSIADARHILLSGADKVSVNTAAVRRPELITELSRELGAQAVVLAIDARRHGPGGWHVYTRGGRDDEGMDAVAWAAR
GEALGAGEVLLTSMDTDGVQDGFDCALTMAVSRATHIPVIASGGAGKPEHFVRVLTDGCADAALAASIFHYGTYTVNQLK
EALDKRGIPVRVTA

Sequences:

>Translated_254_residues
MLAKRIIPCLDVTGGRVVKGVNFVNLRDAGDPVELADRYNLDGADELVFLDITASSDARDIMADVVARTARKVFIPLAVG
GGIRSIADARHILLSGADKVSVNTAAVRRPELITELSRELGAQAVVLAIDARRHGPGGWHVYTRGGRDDEGMDAVAWAAR
GEALGAGEVLLTSMDTDGVQDGFDCALTMAVSRATHIPVIASGGAGKPEHFVRVLTDGCADAALAASIFHYGTYTVNQLK
EALDKRGIPVRVTA
>Mature_254_residues
MLAKRIIPCLDVTGGRVVKGVNFVNLRDAGDPVELADRYNLDGADELVFLDITASSDARDIMADVVARTARKVFIPLAVG
GGIRSIADARHILLSGADKVSVNTAAVRRPELITELSRELGAQAVVLAIDARRHGPGGWHVYTRGGRDDEGMDAVAWAAR
GEALGAGEVLLTSMDTDGVQDGFDCALTMAVSRATHIPVIASGGAGKPEHFVRVLTDGCADAALAASIFHYGTYTVNQLK
EALDKRGIPVRVTA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=45.136186770428, Blast_Score=210, Evalue=9e-56,
Organism=Escherichia coli, GI87082028, Length=205, Percent_Identity=29.7560975609756, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=38.2165605095541, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_SOLUE (Q01ZU6)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_825104.1
- ProteinModelPortal:   Q01ZU6
- SMR:   Q01ZU6
- STRING:   Q01ZU6
- GeneID:   4426650
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_3850
- NMPDR:   fig|234267.9.peg.3659
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q01ZU6
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26769; Mature: 26769

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTGGRVVKGVNFVNLRDAGDPVELADRYNLDGADELVFLDITASSDARD
CCHHHCCCCCCCCCCEEEECEEEEEECCCCCCHHHHHHCCCCCCCCEEEEEEECCCCHHH
IMADVVARTARKVFIPLAVGGGIRSIADARHILLSGADKVSVNTAAVRRPELITELSREL
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCEEECHHHHCCHHHHHHHHHHH
GAQAVVLAIDARRHGPGGWHVYTRGGRDDEGMDAVAWAARGEALGAGEVLLTSMDTDGVQ
CCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCC
DGFDCALTMAVSRATHIPVIASGGAGKPEHFVRVLTDGCADAALAASIFHYGTYTVNQLK
CCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCEEHHHHH
EALDKRGIPVRVTA
HHHHHCCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVTGGRVVKGVNFVNLRDAGDPVELADRYNLDGADELVFLDITASSDARD
CCHHHCCCCCCCCCCEEEECEEEEEECCCCCCHHHHHHCCCCCCCCEEEEEEECCCCHHH
IMADVVARTARKVFIPLAVGGGIRSIADARHILLSGADKVSVNTAAVRRPELITELSREL
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCEEECHHHHCCHHHHHHHHHHH
GAQAVVLAIDARRHGPGGWHVYTRGGRDDEGMDAVAWAARGEALGAGEVLLTSMDTDGVQ
CCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCC
DGFDCALTMAVSRATHIPVIASGGAGKPEHFVRVLTDGCADAALAASIFHYGTYTVNQLK
CCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCEEHHHHH
EALDKRGIPVRVTA
HHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA