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The map label for this gene is gltB [H]

Identifier: 116622914

GI number: 116622914

Start: 4841253

End: 4842653

Strand: Reverse

Name: gltB [H]

Synonym: Acid_3815

Alternate gene names: 116622914

Gene position: 4842653-4841253 (Counterclockwise)

Preceding gene: 116622915

Following gene: 116622908

Centisome position: 48.59

GC content: 64.31

Gene sequence:

>1401_bases
GTGGGTAAGGTAACCGGCTTCCTCGAATACACGCGCGAACTGCCGACGCGGCGCGACCCTGCGGAGCGCATCAAGGATTG
GTTCGAGATCTACAATCCCTTTCCCGAAGAAAGCGTCAAGACGCAGGGCGCGCGCTGCATGGATTGCGGCGTGCCCTTTT
GCCATACCGGCTGCCCGCTGACCAACATCATTCCGGATTGGAATGACCTGGTCTATCGCGGCCGCTGGAAGGAAGCCGCC
CGGCAGCTGCACGCCACCAACAACTTCCCCGAATTCACCGGACGCATCTGTCCCGCGCCTTGCGAAGCCGCATGCGTTCT
CGGCATCAACGAGCCGCCGGTGGCCATCAAGCAGATCGAGAAGACCATCATCGATCGCGCTTTTGCGGAGGGCTACATCC
GTCCCGAACCGGCGCAGGTGCGCACCGGCAAGCGCGTCGCCGTGATCGGGTCGGGACCCGCCGGCCTCGCCGCCGCGCAG
CAGCTCGCGCGCGCCGGCCACGATGTCACGGTCTATGAAAAGAACGACCGCATCGGCGGCCTGCTGCGCTACGGCATCCC
GAATTTCAAGATGGAGAAGCACCTCATCGATCGCCGTCTGGAACAGATGCTCGGCGAAGGGGTGCGCTTTGAGACCAACG
CGCACGTGGGCGGCAATGTCTCGGTGGAAGACCTGCGGCACAGGTATGACGCCATCCTGCTGGCCGGCGGCGCCGAGCAG
GCACGCAATCTGAATGTCCCCGGCCGCGAACTCAAGGGCATACACTTCGCGATGGAGTTCCTGCCGCAACAGAATCGCGT
TTGCGAAGGCGACACCGTGGCCAACCAGATCATGGCCACCGGCAAAAACGTGATCATCATCGGCGGCGGCGATACCGGCG
CCGATTGCCTCGGCACCAGTCATCGCCAGGGCGCGAAATCCGTGACGCAATTCGAACTGCTGCCCAAGCCGCCGGACACG
CGCTCGCCCGCGACTCCGTGGCCGCTATGGCCGATGCAATTGCGCGTAGAAAGCTCGCATGAAGAAGGCGGCACGCGCGA
TTGGAGCGTCAGCACCGTCAGCTTCAGTGGCGACGCCGGGGGCAATGTTAAGAAGCTGCATGCCGTGCGCGTCGGTCCTC
CGCCCAAGGTGGAAGCCGTCGCCGGGACGGAGTTCACGCTGGATTGCGATCTGGTGCTGCTGGCCATGGGCTTCACCGGC
CCAGTGAAGAACGGCATGCTCGAACAACTTGGCGTAGAGTTGGATCTCCGCGGCAACGTGGCCGCCGATGCCAACCACAT
GTCGAGCGTCCCCGGCGTCTTCGCAGCCGGCGACATGCGCCGCGGCCAATCGCTCGTCGTCTGGGCCATCGCCGAAGGCC
GCAAAGCCGCCGCAGGCATCGACCGCTATTTGAAAGCCTGA

Upstream 100 bases:

>100_bases
GAGAACTGGGAGAGCATGCTGCCGAAATTCATCAAGGTCTTCCCGCAGGAGTATCGCAAGGTATTGCAAACTCGGGCCTC
GCGGGTCCTGGAGGTGTCCC

Downstream 100 bases:

>100_bases
GGATGTCCTCAACGAGCCGCGACCGTGAGGGAGCGCTACCCTCACACTACCAGTTGCAACGCTCCCTCAGACTCACGGTC
GCGGCTCGTTGGGCGCTCGC

Product: glutamate synthase (NADH) small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MGKVTGFLEYTRELPTRRDPAERIKDWFEIYNPFPEESVKTQGARCMDCGVPFCHTGCPLTNIIPDWNDLVYRGRWKEAA
RQLHATNNFPEFTGRICPAPCEAACVLGINEPPVAIKQIEKTIIDRAFAEGYIRPEPAQVRTGKRVAVIGSGPAGLAAAQ
QLARAGHDVTVYEKNDRIGGLLRYGIPNFKMEKHLIDRRLEQMLGEGVRFETNAHVGGNVSVEDLRHRYDAILLAGGAEQ
ARNLNVPGRELKGIHFAMEFLPQQNRVCEGDTVANQIMATGKNVIIIGGGDTGADCLGTSHRQGAKSVTQFELLPKPPDT
RSPATPWPLWPMQLRVESSHEEGGTRDWSVSTVSFSGDAGGNVKKLHAVRVGPPPKVEAVAGTEFTLDCDLVLLAMGFTG
PVKNGMLEQLGVELDLRGNVAADANHMSSVPGVFAAGDMRRGQSLVVWAIAEGRKAAAGIDRYLKA

Sequences:

>Translated_466_residues
MGKVTGFLEYTRELPTRRDPAERIKDWFEIYNPFPEESVKTQGARCMDCGVPFCHTGCPLTNIIPDWNDLVYRGRWKEAA
RQLHATNNFPEFTGRICPAPCEAACVLGINEPPVAIKQIEKTIIDRAFAEGYIRPEPAQVRTGKRVAVIGSGPAGLAAAQ
QLARAGHDVTVYEKNDRIGGLLRYGIPNFKMEKHLIDRRLEQMLGEGVRFETNAHVGGNVSVEDLRHRYDAILLAGGAEQ
ARNLNVPGRELKGIHFAMEFLPQQNRVCEGDTVANQIMATGKNVIIIGGGDTGADCLGTSHRQGAKSVTQFELLPKPPDT
RSPATPWPLWPMQLRVESSHEEGGTRDWSVSTVSFSGDAGGNVKKLHAVRVGPPPKVEAVAGTEFTLDCDLVLLAMGFTG
PVKNGMLEQLGVELDLRGNVAADANHMSSVPGVFAAGDMRRGQSLVVWAIAEGRKAAAGIDRYLKA
>Mature_465_residues
GKVTGFLEYTRELPTRRDPAERIKDWFEIYNPFPEESVKTQGARCMDCGVPFCHTGCPLTNIIPDWNDLVYRGRWKEAAR
QLHATNNFPEFTGRICPAPCEAACVLGINEPPVAIKQIEKTIIDRAFAEGYIRPEPAQVRTGKRVAVIGSGPAGLAAAQQ
LARAGHDVTVYEKNDRIGGLLRYGIPNFKMEKHLIDRRLEQMLGEGVRFETNAHVGGNVSVEDLRHRYDAILLAGGAEQA
RNLNVPGRELKGIHFAMEFLPQQNRVCEGDTVANQIMATGKNVIIIGGGDTGADCLGTSHRQGAKSVTQFELLPKPPDTR
SPATPWPLWPMQLRVESSHEEGGTRDWSVSTVSFSGDAGGNVKKLHAVRVGPPPKVEAVAGTEFTLDCDLVLLAMGFTGP
VKNGMLEQLGVELDLRGNVAADANHMSSVPGVFAAGDMRRGQSLVVWAIAEGRKAAAGIDRYLKA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=285, Percent_Identity=25.6140350877193, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI111118983, Length=153, Percent_Identity=32.6797385620915, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI111118981, Length=153, Percent_Identity=32.6797385620915, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1789606, Length=479, Percent_Identity=37.9958246346555, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1788811, Length=484, Percent_Identity=35.9504132231405, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI87082180, Length=455, Percent_Identity=33.8461538461538, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1788468, Length=428, Percent_Identity=28.5046728971963, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789243, Length=434, Percent_Identity=27.4193548387097, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=486, Percent_Identity=49.3827160493827, Blast_Score=458, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71984108, Length=463, Percent_Identity=22.8941684665227, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17543860, Length=157, Percent_Identity=32.484076433121, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=481, Percent_Identity=47.8170478170478, Blast_Score=445, Evalue=1e-126,
Organism=Drosophila melanogaster, GI28574881, Length=481, Percent_Identity=54.6777546777547, Blast_Score=515, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24665539, Length=481, Percent_Identity=54.6777546777547, Blast_Score=515, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24665547, Length=481, Percent_Identity=54.6777546777547, Blast_Score=513, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24665543, Length=481, Percent_Identity=54.6777546777547, Blast_Score=513, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24640763, Length=301, Percent_Identity=26.2458471760797, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI18858217, Length=301, Percent_Identity=26.2458471760797, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17137178, Length=156, Percent_Identity=31.4102564102564, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 50736; Mature: 50605

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVTGFLEYTRELPTRRDPAERIKDWFEIYNPFPEESVKTQGARCMDCGVPFCHTGCPL
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCHHCCCCCH
TNIIPDWNDLVYRGRWKEAARQLHATNNFPEFTGRICPAPCEAACVLGINEPPVAIKQIE
HHCCCCHHHHHCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEECCCCCCHHHHHHH
KTIIDRAFAEGYIRPEPAQVRTGKRVAVIGSGPAGLAAAQQLARAGHDVTVYEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPNFKMEKHLIDRRLEQMLGEGVRFETNAHVGGNVSVEDLRHRYDAILLAGGAEQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCEEEEECCHHH
ARNLNVPGRELKGIHFAMEFLPQQNRVCEGDTVANQIMATGKNVIIIGGGDTGADCLGTS
HCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHCCCC
HRQGAKSVTQFELLPKPPDTRSPATPWPLWPMQLRVESSHEEGGTRDWSVSTVSFSGDAG
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCC
GNVKKLHAVRVGPPPKVEAVAGTEFTLDCDLVLLAMGFTGPVKNGMLEQLGVELDLRGNV
CCEEEEEEEECCCCCCEEEECCCEEEECCCEEEEECCCCCCCCCCHHHHHCCEEEECCCC
AADANHMSSVPGVFAAGDMRRGQSLVVWAIAEGRKAAAGIDRYLKA
CCCCHHHHCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GKVTGFLEYTRELPTRRDPAERIKDWFEIYNPFPEESVKTQGARCMDCGVPFCHTGCPL
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCHHCCCCCH
TNIIPDWNDLVYRGRWKEAARQLHATNNFPEFTGRICPAPCEAACVLGINEPPVAIKQIE
HHCCCCHHHHHCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEECCCCCCHHHHHHH
KTIIDRAFAEGYIRPEPAQVRTGKRVAVIGSGPAGLAAAQQLARAGHDVTVYEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPNFKMEKHLIDRRLEQMLGEGVRFETNAHVGGNVSVEDLRHRYDAILLAGGAEQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCEEEEECCHHH
ARNLNVPGRELKGIHFAMEFLPQQNRVCEGDTVANQIMATGKNVIIIGGGDTGADCLGTS
HCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHCCCC
HRQGAKSVTQFELLPKPPDTRSPATPWPLWPMQLRVESSHEEGGTRDWSVSTVSFSGDAG
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCC
GNVKKLHAVRVGPPPKVEAVAGTEFTLDCDLVLLAMGFTGPVKNGMLEQLGVELDLRGNV
CCEEEEEEEECCCCCCEEEECCCEEEECCCEEEEECCCCCCCCCCHHHHHCCEEEECCCC
AADANHMSSVPGVFAAGDMRRGQSLVVWAIAEGRKAAAGIDRYLKA
CCCCHHHHCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]