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The map label for this gene is cofH [H]

Identifier: 116622715

GI number: 116622715

Start: 4573217

End: 4574248

Strand: Reverse

Name: cofH [H]

Synonym: Acid_3614

Alternate gene names: 116622715

Gene position: 4574248-4573217 (Counterclockwise)

Preceding gene: 116622716

Following gene: 116622714

Centisome position: 45.9

GC content: 61.14

Gene sequence:

>1032_bases
ATGATCACGCGCGAAGAAGCTCTCGATCTGTTCCGCTCGGACGATCTTATCGGCATCGGTATGGAAGCCGACCGCATCCG
CCGTCGGTGGCATCCCGAAAACGTCGTCAGCTACATCATCGATCGCAATATCAATTACACCAATTTCTGCACGGAATACT
GTAGCTTCTGCGCCTTCTACCGTCCGCCAGGCCACGCCGAAGGCTACATCCTTCCCAACGAAGTGATCTACGAGAAAATC
CAGGAGACGCTCGATCTCGGCGGCACCGGAGTGTTGATGCAGGGTGGCCTCCATCCCGATCTTAAGATCGAGTGGTATGA
AGATCTCTTCCGCGGCATCAAGGCGCGCTTTCCGATTCACCTGCACTGCCTCTCCGCCCCCGAAGTCACCAACATCGCCG
AGGTCAGCGGCATCTCCCTGCGCGACACCATCGCCCGCCTGCGCGACGCCGGCCTCGATTCCATTCCCGGCGGCGGCGCG
GAGATCCTCGATGACGCCGTTCGCCATCGCATCAGCCGCCTCAAGTGCGGTACCCAGGATTGGATGGACGTGCACCGCAC
CGCCCATCAGCTCGGCATGCGCACCACCGCGACCATGATGTTCGGCTGCGGTGAGACTCTCGAGCAGCGCATGAATCACC
TGGAACTGGTGCGCGGGCTGCAGGAAGAGACCGGCGGCTTCACGGCCTTCATCCCCTGGACTTTCCAGCGCGAGAACACG
TCGCTCGGCCGCTTCGTCAAGGAAGAGGCCACCGCGGTCGAATACCTGCAGACTCTGGCTATCAGCCGGATCTATCTCGA
TAACTTCGAGAATGTGCAGTCCAGCTGGGTGACCCAGGGACTCAAGACCTGCCAGCTCGGCCTGCGCTTCGGCGGCAACG
ATGTCGGCAGCATCATGATCGAAGAGAACGTGGTTTCCGCCGCCGGCGCCCACCACCGCGCCAGCGAAGAGGAACTCCGG
CGGATCATCCGCGATGCCGGCTTCGTTCCCAAGCAGCGCGATACCCTCTACCGCACCTACTTCCTGAACTAA

Upstream 100 bases:

>100_bases
CCGATGAAGCAGCAGTTGGCCGCTGTCGGCGCCTAAAAACGTGGATTGCTGACCTGGGATGGCTTGCCTGCTATCCTGAA
GGGAATCGCACTCTACCCCT

Downstream 100 bases:

>100_bases
GTACACGGTTTCAGAGGCTCGTGCCTTGTTGGATAGACCTGGCGCCTGCTGGCGTATTCTCTCATTTTCGACATACGATG
ATAGAAAGATGACGCAACCG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MITREEALDLFRSDDLIGIGMEADRIRRRWHPENVVSYIIDRNINYTNFCTEYCSFCAFYRPPGHAEGYILPNEVIYEKI
QETLDLGGTGVLMQGGLHPDLKIEWYEDLFRGIKARFPIHLHCLSAPEVTNIAEVSGISLRDTIARLRDAGLDSIPGGGA
EILDDAVRHRISRLKCGTQDWMDVHRTAHQLGMRTTATMMFGCGETLEQRMNHLELVRGLQEETGGFTAFIPWTFQRENT
SLGRFVKEEATAVEYLQTLAISRIYLDNFENVQSSWVTQGLKTCQLGLRFGGNDVGSIMIEENVVSAAGAHHRASEEELR
RIIRDAGFVPKQRDTLYRTYFLN

Sequences:

>Translated_343_residues
MITREEALDLFRSDDLIGIGMEADRIRRRWHPENVVSYIIDRNINYTNFCTEYCSFCAFYRPPGHAEGYILPNEVIYEKI
QETLDLGGTGVLMQGGLHPDLKIEWYEDLFRGIKARFPIHLHCLSAPEVTNIAEVSGISLRDTIARLRDAGLDSIPGGGA
EILDDAVRHRISRLKCGTQDWMDVHRTAHQLGMRTTATMMFGCGETLEQRMNHLELVRGLQEETGGFTAFIPWTFQRENT
SLGRFVKEEATAVEYLQTLAISRIYLDNFENVQSSWVTQGLKTCQLGLRFGGNDVGSIMIEENVVSAAGAHHRASEEELR
RIIRDAGFVPKQRDTLYRTYFLN
>Mature_343_residues
MITREEALDLFRSDDLIGIGMEADRIRRRWHPENVVSYIIDRNINYTNFCTEYCSFCAFYRPPGHAEGYILPNEVIYEKI
QETLDLGGTGVLMQGGLHPDLKIEWYEDLFRGIKARFPIHLHCLSAPEVTNIAEVSGISLRDTIARLRDAGLDSIPGGGA
EILDDAVRHRISRLKCGTQDWMDVHRTAHQLGMRTTATMMFGCGETLEQRMNHLELVRGLQEETGGFTAFIPWTFQRENT
SLGRFVKEEATAVEYLQTLAISRIYLDNFENVQSSWVTQGLKTCQLGLRFGGNDVGSIMIEENVVSAAGAHHRASEEELR
RIIRDAGFVPKQRDTLYRTYFLN

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39025; Mature: 39025

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITREEALDLFRSDDLIGIGMEADRIRRRWHPENVVSYIIDRNINYTNFCTEYCSFCAFY
CCCHHHHHHHHHCCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
RPPGHAEGYILPNEVIYEKIQETLDLGGTGVLMQGGLHPDLKIEWYEDLFRGIKARFPIH
CCCCCCCCEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHCCCEE
LHCLSAPEVTNIAEVSGISLRDTIARLRDAGLDSIPGGGAEILDDAVRHRISRLKCGTQD
EEEECCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
WMDVHRTAHQLGMRTTATMMFGCGETLEQRMNHLELVRGLQEETGGFTAFIPWTFQRENT
HHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCC
SLGRFVKEEATAVEYLQTLAISRIYLDNFENVQSSWVTQGLKTCQLGLRFGGNDVGSIMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EENVVSAAGAHHRASEEELRRIIRDAGFVPKQRDTLYRTYFLN
ECHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHEECC
>Mature Secondary Structure
MITREEALDLFRSDDLIGIGMEADRIRRRWHPENVVSYIIDRNINYTNFCTEYCSFCAFY
CCCHHHHHHHHHCCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
RPPGHAEGYILPNEVIYEKIQETLDLGGTGVLMQGGLHPDLKIEWYEDLFRGIKARFPIH
CCCCCCCCEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHCCCEE
LHCLSAPEVTNIAEVSGISLRDTIARLRDAGLDSIPGGGAEILDDAVRHRISRLKCGTQD
EEEECCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
WMDVHRTAHQLGMRTTATMMFGCGETLEQRMNHLELVRGLQEETGGFTAFIPWTFQRENT
HHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCC
SLGRFVKEEATAVEYLQTLAISRIYLDNFENVQSSWVTQGLKTCQLGLRFGGNDVGSIMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EENVVSAAGAHHRASEEELRRIIRDAGFVPKQRDTLYRTYFLN
ECHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]