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The map label for this gene is gatA [H]

Identifier: 116622599

GI number: 116622599

Start: 4426522

End: 4427877

Strand: Reverse

Name: gatA [H]

Synonym: Acid_3497

Alternate gene names: 116622599

Gene position: 4427877-4426522 (Counterclockwise)

Preceding gene: 116622619

Following gene: 116622594

Centisome position: 44.43

GC content: 65.78

Gene sequence:

>1356_bases
ATGAACGAACTCCTCAGGCTTTCCGCGACGCAACAGGCACGCCTCATTCGGGAGCGTGAGATTTCTTCGGTTGAATTGGT
GGACGCGCATCTGCGCTGGATCGGAGTCGTGAATCCGCGCATCAACGCGGCGATCGACGTACTTGCCGATTCAGCGCTGG
CGGCGGCGCGGCGCGCCGATGAGAGCGAGGCGCGAGGTCCGCTTCACGGCGTGCCATTCAGTATCAAGGATTCTCTCGAA
CTGGCCGGATCGGTGTGCACTGCAGGCACGGTGGGTCGCCGTTGCGCGGCTCCCGCGACCGAAGATGCAGTCCTGATTTC
ACGGCTGCGCGCGGCAGGCGCGATCCCGATTGCGCGGACGAATCTGCCCGATCTCCTCTTCGCCTTCGAGAGCGACAACC
TGCTCTACGGCGCCACGCGGAATCCGTACGATGGATCGCGGACGTCGGGAGGCAGCAGCGGCGGGGAGGCGGCCCTGATT
GCCAGTTGCGGTTCGCCGCTCGGACTTGGCAGCGATGCCGCGGGGAGCGTCCGCCTGCCCGCGGCGTTTTGCGGAATCGC
CGGCATCAAGCCGACGTCGGGGCGACTGGATCGCACCGGTCACTTTCCGCCGGCGGGAGGTTGGATCGAAGCGCTCTGGC
AGATCGGACCGATGGCACGGTACGTCGAAGACCTGCAGACGGTGATGCCGCTGCTTATCGGAGAACCTGTACGCTCCTCT
CCGGATTTAGGGACACTGCGCGCGGCCTTCTACAGCGGCCCCGCGGATGCCGAGGTGGAGGCGGTGGTGCGTGGCGCCGC
GCAGTCGCTCGCTGGGGCGGTCGCGCGGATCGATGAGGATCGCCCGCCGTGTCTCGACCAGGCATTCGACCTCGAGATGA
AATTGCTGGGTCCCGATGGCGGGGACGGCCTTCGGCAGTACCTGTGCGATCTGGGCTCCAGGGAGGTGCACCCGCTGCTC
ACCGCGTGGCTCGACAAGCTTGAGCGGTACCGCACCGACCTTGCCGGTTTTGCGGGGTATTGGGCGGAATGGGATGCGTA
TCGCGCCGCGATGTCGGCGTTTCTGCGGCGCTATGACGTGATTCTGTGCCCGGTCTATCCGCACGCTGCCCTCCGGCACG
GAAGTTCGGTGGAAGACGAGAATTTCCGCGGCTTCGCGCATACCATGGCGTACAACGTGGCCGGACTACCGGCAGCCGTA
GTGCGCTGCGGACAATCGGCGGACGGGCTCCCGATTGCGGTTCAGATCGTCGCGGGACCTGGGCGCGAAGATGTAGCGCT
GGGCGTTGCGGCGTTTCTGGAAGGGGAATTCGGTGGATGGAAGGCGCCGGAGTTTGCGCAGGTTTTCGCCGGCTGA

Upstream 100 bases:

>100_bases
ACTCGGTGTCACCCATTGTGGAAGTCCCAGACCGCCGAATCATATCAGGAGCGCCGATGTGGCACACTATTGTTCGCGAA
CGTTGTTCTCGAACCCAGGT

Downstream 100 bases:

>100_bases
AAACCGGCTGCACGGTCGAAAGCCCCCACGGCGAACAACGGAATACTCCTCGAAAAGCGCGGCGTAGCTGACGCGCAATT
TGGAGGCGCTGGCGGTCTCC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNELLRLSATQQARLIREREISSVELVDAHLRWIGVVNPRINAAIDVLADSALAAARRADESEARGPLHGVPFSIKDSLE
LAGSVCTAGTVGRRCAAPATEDAVLISRLRAAGAIPIARTNLPDLLFAFESDNLLYGATRNPYDGSRTSGGSSGGEAALI
ASCGSPLGLGSDAAGSVRLPAAFCGIAGIKPTSGRLDRTGHFPPAGGWIEALWQIGPMARYVEDLQTVMPLLIGEPVRSS
PDLGTLRAAFYSGPADAEVEAVVRGAAQSLAGAVARIDEDRPPCLDQAFDLEMKLLGPDGGDGLRQYLCDLGSREVHPLL
TAWLDKLERYRTDLAGFAGYWAEWDAYRAAMSAFLRRYDVILCPVYPHAALRHGSSVEDENFRGFAHTMAYNVAGLPAAV
VRCGQSADGLPIAVQIVAGPGREDVALGVAAFLEGEFGGWKAPEFAQVFAG

Sequences:

>Translated_451_residues
MNELLRLSATQQARLIREREISSVELVDAHLRWIGVVNPRINAAIDVLADSALAAARRADESEARGPLHGVPFSIKDSLE
LAGSVCTAGTVGRRCAAPATEDAVLISRLRAAGAIPIARTNLPDLLFAFESDNLLYGATRNPYDGSRTSGGSSGGEAALI
ASCGSPLGLGSDAAGSVRLPAAFCGIAGIKPTSGRLDRTGHFPPAGGWIEALWQIGPMARYVEDLQTVMPLLIGEPVRSS
PDLGTLRAAFYSGPADAEVEAVVRGAAQSLAGAVARIDEDRPPCLDQAFDLEMKLLGPDGGDGLRQYLCDLGSREVHPLL
TAWLDKLERYRTDLAGFAGYWAEWDAYRAAMSAFLRRYDVILCPVYPHAALRHGSSVEDENFRGFAHTMAYNVAGLPAAV
VRCGQSADGLPIAVQIVAGPGREDVALGVAAFLEGEFGGWKAPEFAQVFAG
>Mature_451_residues
MNELLRLSATQQARLIREREISSVELVDAHLRWIGVVNPRINAAIDVLADSALAAARRADESEARGPLHGVPFSIKDSLE
LAGSVCTAGTVGRRCAAPATEDAVLISRLRAAGAIPIARTNLPDLLFAFESDNLLYGATRNPYDGSRTSGGSSGGEAALI
ASCGSPLGLGSDAAGSVRLPAAFCGIAGIKPTSGRLDRTGHFPPAGGWIEALWQIGPMARYVEDLQTVMPLLIGEPVRSS
PDLGTLRAAFYSGPADAEVEAVVRGAAQSLAGAVARIDEDRPPCLDQAFDLEMKLLGPDGGDGLRQYLCDLGSREVHPLL
TAWLDKLERYRTDLAGFAGYWAEWDAYRAAMSAFLRRYDVILCPVYPHAALRHGSSVEDENFRGFAHTMAYNVAGLPAAV
VRCGQSADGLPIAVQIVAGPGREDVALGVAAFLEGEFGGWKAPEFAQVFAG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=485, Percent_Identity=31.1340206185567, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI166795287, Length=472, Percent_Identity=30.9322033898305, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=24.3951612903226, Blast_Score=83, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=485, Percent_Identity=30.3092783505155, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17538252, Length=489, Percent_Identity=28.2208588957055, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17556264, Length=202, Percent_Identity=40.0990099009901, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71990152, Length=200, Percent_Identity=36, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538254, Length=510, Percent_Identity=25.0980392156863, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17556278, Length=239, Percent_Identity=34.7280334728033, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17556276, Length=239, Percent_Identity=34.7280334728033, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32565337, Length=450, Percent_Identity=24, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=163, Percent_Identity=29.4478527607362, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320448, Length=222, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319685, Length=173, Percent_Identity=34.1040462427746, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24644968, Length=491, Percent_Identity=30.7535641547862, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=31.1244979919679, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=31.1244979919679, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=31.1244979919679, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=31.1244979919679, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI161078093, Length=497, Percent_Identity=30.784708249497, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21356731, Length=295, Percent_Identity=37.6271186440678, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI45550774, Length=245, Percent_Identity=40.4081632653061, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24648435, Length=245, Percent_Identity=40.4081632653061, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24648437, Length=245, Percent_Identity=40.4081632653061, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24648441, Length=177, Percent_Identity=41.8079096045198, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24648439, Length=177, Percent_Identity=41.8079096045198, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24648113, Length=261, Percent_Identity=31.0344827586207, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 47693; Mature: 47693

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELLRLSATQQARLIREREISSVELVDAHLRWIGVVNPRINAAIDVLADSALAAARRAD
CCCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCC
ESEARGPLHGVPFSIKDSLELAGSVCTAGTVGRRCAAPATEDAVLISRLRAAGAIPIART
HHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECC
NLPDLLFAFESDNLLYGATRNPYDGSRTSGGSSGGEAALIASCGSPLGLGSDAAGSVRLP
CCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEECC
AAFCGIAGIKPTSGRLDRTGHFPPAGGWIEALWQIGPMARYVEDLQTVMPLLIGEPVRSS
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCC
PDLGTLRAAFYSGPADAEVEAVVRGAAQSLAGAVARIDEDRPPCLDQAFDLEMKLLGPDG
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEECCCC
GDGLRQYLCDLGSREVHPLLTAWLDKLERYRTDLAGFAGYWAEWDAYRAAMSAFLRRYDV
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
ILCPVYPHAALRHGSSVEDENFRGFAHTMAYNVAGLPAAVVRCGQSADGLPIAVQIVAGP
EEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEEEECC
GREDVALGVAAFLEGEFGGWKAPEFAQVFAG
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MNELLRLSATQQARLIREREISSVELVDAHLRWIGVVNPRINAAIDVLADSALAAARRAD
CCCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCC
ESEARGPLHGVPFSIKDSLELAGSVCTAGTVGRRCAAPATEDAVLISRLRAAGAIPIART
HHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECC
NLPDLLFAFESDNLLYGATRNPYDGSRTSGGSSGGEAALIASCGSPLGLGSDAAGSVRLP
CCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEECC
AAFCGIAGIKPTSGRLDRTGHFPPAGGWIEALWQIGPMARYVEDLQTVMPLLIGEPVRSS
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCC
PDLGTLRAAFYSGPADAEVEAVVRGAAQSLAGAVARIDEDRPPCLDQAFDLEMKLLGPDG
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEECCCC
GDGLRQYLCDLGSREVHPLLTAWLDKLERYRTDLAGFAGYWAEWDAYRAAMSAFLRRYDV
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
ILCPVYPHAALRHGSSVEDENFRGFAHTMAYNVAGLPAAVVRCGQSADGLPIAVQIVAGP
EEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEEEECC
GREDVALGVAAFLEGEFGGWKAPEFAQVFAG
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA