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The map label for this gene is pckA1 [H]

Identifier: 116622570

GI number: 116622570

Start: 4385885

End: 4387504

Strand: Direct

Name: pckA1 [H]

Synonym: Acid_3468

Alternate gene names: 116622570

Gene position: 4385885-4387504 (Clockwise)

Preceding gene: 116622567

Following gene: 116622571

Centisome position: 44.01

GC content: 62.59

Gene sequence:

>1620_bases
ATGCTGATTGAACACAAGCAGATTGCGAATGCGGGGATTCGGCCAAGCCGCCACGGTCTCGAACAGCAGGGATTCCGGAA
TCTGAATGTCGCTTACTGGAACCTCGGCACGGCGCAACTGCTGGAACACGCGATCCAGCGGAGGGAAGGCTCCTTCGCCA
GCGGCGGCTCGTTCGTAGTCCGCACCGGCCAGTTCACCGGGCGCTCGCCCAAGGATAAATTCATTGTGCGCGATGAGATC
ACCGATGCGCACGTGCATTGGGGGCCGGTCAACCAGCCGCTCTCCGAAGCGCATTTCGACCGCCTGTATACCAAAATGAT
GGCGTTCTGGCAGGGCCACGACGTCTATGTGCAGGATTGCTATGTGGGCGCCGACCGCGAGCTCGGCATGCCGATCCGGG
TGGTCAGCCAGCTGGCCTGGCACAACCTGTTCGCGCGGCAGTTGTTCATACGGCCCGATCCGGAGCTGACCGGCGAGCAC
AAGCCGCAGTTTTCGATTTTCTTCGCGCCGGACTTCCAGGCCAATCCGGCCGAGGATGGGACCAACTCGGAGACGTGCAT
CGTCATCAATTTCAAGAAAAAGGTGGTGCTGATCTGCGGCACGAGCTACGCCGGGGAGATGAAGAAATCCGCCTTCACGA
TCATGAACTACCTGCTGCCGGGGCAGGGAGTTTTCCCCATGCACTGCTCTTCGAACATGGGCGCGGCGGGCGATACCGCG
CTCTTCTTCGGACTCTCGGGCACCGGCAAGACCACACTCTCCGCGGACCCGGAGCGCCGCCTGATCGGCGACGATGAGCA
CGGCTGGAGCGACAACGGCGTCTTCAATTTCGAGGGCGGCTGCTACGCCAAGTGCATCCGGCTCTCGGCCGAAAACGAGC
CGCAGATCTGGAATGCGATCCGCTTCGGCACCGTGCTCGAGAACGTCGCGATGGATTCGGAAACGCGGCTGCTCGATTTC
AACTCCGATGAAGTCACGGAGAATACGCGTGCCGCCTACCCGCTGAAGTTCATCGAGGGCGCGATCATTCCGAGCGTGGG
CGGCCATCCGAATCACGTGATCTTTTTGACCGCCGATGCCTTCGGCGTGCTGCCGCCGATTTCGAAGCTGACTCCGGAGC
AGGCCATGTACCACTTCCTCAGCGGGTACACGGCGAAGGTCGCCGGTACGGAGCGCGGCCTCGGCAAGGAGCCGCAGGCC
ACGTTCAGCGCGTGCTTCGGCGCGCCCTTCCTGCCGCGTCCGGCCGCTGCTTACGCGTCCCTGCTCGGCGAGAAACTCCG
GCGCCACGGAGCGCAGGTCTGGTTGGTGAATACCGGCTGGGTAGGCGGCCCTTACGGCGTGGGAGAGCGCATGAAGCTGC
GTTACACGCGCGCGATGCTGCAGGCCGCGTTAAGCGGTCAACTCAACGATGTGGCCACGGAGCAGCACCCGATCTTCGGC
GTGGCCGTGCCCAAGAGCTGCCCGAATGTTCCGGCAACCTTCTTGGATGCGCGTGGGATGTGGAGCGATAAGACGGCGTA
CGATCGCGCAGCGCAGGATCTGGCCTCGCGTTTCAACAAGAACTTCGAAAAGTTCAGCGGCGTCAGCCCCGAGATTGCCG
CGGCGGCTCCAGGTAAATAA

Upstream 100 bases:

>100_bases
CGCCCCGCCCACAATATCCACACCGCGGCGCATGTCCAGACCAGCCGGTTCATGAGACAATTCTACGATACGGGCTGGGC
CCGAATGGGAATTAATTACA

Downstream 100 bases:

>100_bases
ATGAGAATCGCGGCTGCGATCGGCCTGCTGGTGTTCTGTGGCGCCGCGCGCGCCCAGCACCCGCTCGATGAAGAACAGAC
TCAGCTGGTGGAGGCGGCGC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase 1; PEPCK; Phosphoenolpyruvate carboxylase 1 [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MLIEHKQIANAGIRPSRHGLEQQGFRNLNVAYWNLGTAQLLEHAIQRREGSFASGGSFVVRTGQFTGRSPKDKFIVRDEI
TDAHVHWGPVNQPLSEAHFDRLYTKMMAFWQGHDVYVQDCYVGADRELGMPIRVVSQLAWHNLFARQLFIRPDPELTGEH
KPQFSIFFAPDFQANPAEDGTNSETCIVINFKKKVVLICGTSYAGEMKKSAFTIMNYLLPGQGVFPMHCSSNMGAAGDTA
LFFGLSGTGKTTLSADPERRLIGDDEHGWSDNGVFNFEGGCYAKCIRLSAENEPQIWNAIRFGTVLENVAMDSETRLLDF
NSDEVTENTRAAYPLKFIEGAIIPSVGGHPNHVIFLTADAFGVLPPISKLTPEQAMYHFLSGYTAKVAGTERGLGKEPQA
TFSACFGAPFLPRPAAAYASLLGEKLRRHGAQVWLVNTGWVGGPYGVGERMKLRYTRAMLQAALSGQLNDVATEQHPIFG
VAVPKSCPNVPATFLDARGMWSDKTAYDRAAQDLASRFNKNFEKFSGVSPEIAAAAPGK

Sequences:

>Translated_539_residues
MLIEHKQIANAGIRPSRHGLEQQGFRNLNVAYWNLGTAQLLEHAIQRREGSFASGGSFVVRTGQFTGRSPKDKFIVRDEI
TDAHVHWGPVNQPLSEAHFDRLYTKMMAFWQGHDVYVQDCYVGADRELGMPIRVVSQLAWHNLFARQLFIRPDPELTGEH
KPQFSIFFAPDFQANPAEDGTNSETCIVINFKKKVVLICGTSYAGEMKKSAFTIMNYLLPGQGVFPMHCSSNMGAAGDTA
LFFGLSGTGKTTLSADPERRLIGDDEHGWSDNGVFNFEGGCYAKCIRLSAENEPQIWNAIRFGTVLENVAMDSETRLLDF
NSDEVTENTRAAYPLKFIEGAIIPSVGGHPNHVIFLTADAFGVLPPISKLTPEQAMYHFLSGYTAKVAGTERGLGKEPQA
TFSACFGAPFLPRPAAAYASLLGEKLRRHGAQVWLVNTGWVGGPYGVGERMKLRYTRAMLQAALSGQLNDVATEQHPIFG
VAVPKSCPNVPATFLDARGMWSDKTAYDRAAQDLASRFNKNFEKFSGVSPEIAAAAPGK
>Mature_539_residues
MLIEHKQIANAGIRPSRHGLEQQGFRNLNVAYWNLGTAQLLEHAIQRREGSFASGGSFVVRTGQFTGRSPKDKFIVRDEI
TDAHVHWGPVNQPLSEAHFDRLYTKMMAFWQGHDVYVQDCYVGADRELGMPIRVVSQLAWHNLFARQLFIRPDPELTGEH
KPQFSIFFAPDFQANPAEDGTNSETCIVINFKKKVVLICGTSYAGEMKKSAFTIMNYLLPGQGVFPMHCSSNMGAAGDTA
LFFGLSGTGKTTLSADPERRLIGDDEHGWSDNGVFNFEGGCYAKCIRLSAENEPQIWNAIRFGTVLENVAMDSETRLLDF
NSDEVTENTRAAYPLKFIEGAIIPSVGGHPNHVIFLTADAFGVLPPISKLTPEQAMYHFLSGYTAKVAGTERGLGKEPQA
TFSACFGAPFLPRPAAAYASLLGEKLRRHGAQVWLVNTGWVGGPYGVGERMKLRYTRAMLQAALSGQLNDVATEQHPIFG
VAVPKSCPNVPATFLDARGMWSDKTAYDRAAQDLASRFNKNFEKFSGVSPEIAAAAPGK

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=544, Percent_Identity=44.3014705882353, Blast_Score=444, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322950, Length=512, Percent_Identity=46.484375, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59250; Mature: 59250

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIEHKQIANAGIRPSRHGLEQQGFRNLNVAYWNLGTAQLLEHAIQRREGSFASGGSFVV
CCCCCHHHHCCCCCCCCCCHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCEEE
RTGQFTGRSPKDKFIVRDEITDAHVHWGPVNQPLSEAHFDRLYTKMMAFWQGHDVYVQDC
EECCCCCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
YVGADRELGMPIRVVSQLAWHNLFARQLFIRPDPELTGEHKPQFSIFFAPDFQANPAEDG
EECCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCC
TNSETCIVINFKKKVVLICGTSYAGEMKKSAFTIMNYLLPGQGVFPMHCSSNMGAAGDTA
CCCCEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCE
LFFGLSGTGKTTLSADPERRLIGDDEHGWSDNGVFNFEGGCYAKCIRLSAENEPQIWNAI
EEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHH
RFGTVLENVAMDSETRLLDFNSDEVTENTRAAYPLKFIEGAIIPSVGGHPNHVIFLTADA
HHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCHHHHCCEECCCCCCCCCEEEEEECCC
FGVLPPISKLTPEQAMYHFLSGYTAKVAGTERGLGKEPQATFSACFGAPFLPRPAAAYAS
CCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
LLGEKLRRHGAQVWLVNTGWVGGPYGVGERMKLRYTRAMLQAALSGQLNDVATEQHPIFG
HHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEE
VAVPKSCPNVPATFLDARGMWSDKTAYDRAAQDLASRFNKNFEKFSGVSPEIAAAAPGK
EECCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLIEHKQIANAGIRPSRHGLEQQGFRNLNVAYWNLGTAQLLEHAIQRREGSFASGGSFVV
CCCCCHHHHCCCCCCCCCCHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCEEE
RTGQFTGRSPKDKFIVRDEITDAHVHWGPVNQPLSEAHFDRLYTKMMAFWQGHDVYVQDC
EECCCCCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
YVGADRELGMPIRVVSQLAWHNLFARQLFIRPDPELTGEHKPQFSIFFAPDFQANPAEDG
EECCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCC
TNSETCIVINFKKKVVLICGTSYAGEMKKSAFTIMNYLLPGQGVFPMHCSSNMGAAGDTA
CCCCEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCE
LFFGLSGTGKTTLSADPERRLIGDDEHGWSDNGVFNFEGGCYAKCIRLSAENEPQIWNAI
EEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHH
RFGTVLENVAMDSETRLLDFNSDEVTENTRAAYPLKFIEGAIIPSVGGHPNHVIFLTADA
HHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCHHHHCCEECCCCCCCCCEEEEEECCC
FGVLPPISKLTPEQAMYHFLSGYTAKVAGTERGLGKEPQATFSACFGAPFLPRPAAAYAS
CCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
LLGEKLRRHGAQVWLVNTGWVGGPYGVGERMKLRYTRAMLQAALSGQLNDVATEQHPIFG
HHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEE
VAVPKSCPNVPATFLDARGMWSDKTAYDRAAQDLASRFNKNFEKFSGVSPEIAAAAPGK
EECCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA