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The map label for this gene is dgoD [H]

Identifier: 116622445

GI number: 116622445

Start: 4231751

End: 4233103

Strand: Direct

Name: dgoD [H]

Synonym: Acid_3343

Alternate gene names: 116622445

Gene position: 4231751-4233103 (Clockwise)

Preceding gene: 116622444

Following gene: 116622446

Centisome position: 42.46

GC content: 59.42

Gene sequence:

>1353_bases
ATGCCGCTGGATCGCACGAGGCCTGCTCGCAGGTCGTTTCTCAAAGGTACTGCCGCCGCCCTTGGATTGGGCTTCGGGGT
GGACAACGTTCTGGAAGCTCTTCCACAAAACGTCAATACCAATTCCAGACCTTCCGAATTAAAAATCACGGACCTGCGGG
TGGCCGTGGTGAGCCGGGCTCCGATGACATGCCCGATCATACGGATCGACACCAATCAGGGAATCTACGGCCTGGGCGAG
GTGCGCGACGGCGCCAGCAAGAACTATGCGCTGATGTTGAAGAGCCGGTTGCTCAACGAGAACCCGTGCAACGTGGACAA
GCTGTTCCGTAAGATCAAACAATTCGGAGGACATGCACGGCAAGGCGGCGGCGTGTGCGGCGTGGAGATGGCGCTGTGGG
ACCTGGCGGGCAAGGCTTACAACGTACCGGTCCACCAGATGCTGGGAGGCAAGTTCCGCGACCGCATTCGCTGTTATGCC
GACACCACGGAATCGCACGATCCGAAGGTCTATGGGCAGCGCCTGAAAGCGCGGCGCGACGAGGGGTTCACGTGGCTGAA
GATGGACCTCGGGGTCGATCTGGTGGCCGATGTTCCGGGAGCGCTCACGATGCCGGTGGGAGAACGGATCAACGGCAACC
CGATGGTCGCGCACATGTTCACCGGCAACGAGATCACCGAGAAGGGGATCCAGTTGATGTGCGATTACGTGGCGCAGATC
CGCGAAATTGTCGGCATGGATATCCCGCTCTCCGCGGACCACTTCGGACATATCGGCGTCAACAGCTGTATTCGTTTGGG
CAAGGCGCTCACGAAGTACAATATGGCCTGGCTCGAGGATATGATTCCGTGGCAGCAGACCGCACTGCTCAAGCAGATCG
CGGACGCAGTGGATATTCCGATTCTCACCGGCGAGGATATCTATCTCAAGGAACCATTCATCGAGTTGTGCAAGGCTCAT
GCGGTAGATATCATTCATCCCGACCTGGCCACTTCGGGCGGCATTCTGGAGACCAAGAAAATCGGTGACGCGGCGCAGGA
ACTCGGCGTCGCGATGGCAATGCACTTTGCGGGCTCGCCCGTCTCCTGCATGGCCAACGTGCACTGCGCGGCCGCCACGG
AGAATTTCCTGGTCCTGGAAAATCATTCGGTGGACGTCCCGTGGTGGGGCGACCTGGTGGAAGGCGTATCGAAGCCGATC
GTGAGCAAGGGCTTTATCACAGTGCCGAACGGCCCGGGGCTCGGCGTCACGCTGAACGACGAAGTGGTCAAGCAGCACCT
GGGTGAGCCGGGTTATTTTGAAGCCACACCGTACTGGAATGTAGACCGGTCCAACGACCGTCAGTGGAGCTGA

Upstream 100 bases:

>100_bases
GAATCAACGCTTCCCCGCTAAGGGGCGCGGGTTGTGTTGCAAACCAAAATAGAATACGATCTGGTTGTCGCTGAGAGGTT
CCGAAAGGGGGATTCACTCG

Downstream 100 bases:

>100_bases
AACCGTATGACTAGACGAAGACTTATCACCTTCCTGGCATCTTGTGCCGTGGGCGCCCTTCTCGCCGTGCCTTCGCACGC
GCAGGTGTACACGCTGACCA

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPLDRTRPARRSFLKGTAAALGLGFGVDNVLEALPQNVNTNSRPSELKITDLRVAVVSRAPMTCPIIRIDTNQGIYGLGE
VRDGASKNYALMLKSRLLNENPCNVDKLFRKIKQFGGHARQGGGVCGVEMALWDLAGKAYNVPVHQMLGGKFRDRIRCYA
DTTESHDPKVYGQRLKARRDEGFTWLKMDLGVDLVADVPGALTMPVGERINGNPMVAHMFTGNEITEKGIQLMCDYVAQI
REIVGMDIPLSADHFGHIGVNSCIRLGKALTKYNMAWLEDMIPWQQTALLKQIADAVDIPILTGEDIYLKEPFIELCKAH
AVDIIHPDLATSGGILETKKIGDAAQELGVAMAMHFAGSPVSCMANVHCAAATENFLVLENHSVDVPWWGDLVEGVSKPI
VSKGFITVPNGPGLGVTLNDEVVKQHLGEPGYFEATPYWNVDRSNDRQWS

Sequences:

>Translated_450_residues
MPLDRTRPARRSFLKGTAAALGLGFGVDNVLEALPQNVNTNSRPSELKITDLRVAVVSRAPMTCPIIRIDTNQGIYGLGE
VRDGASKNYALMLKSRLLNENPCNVDKLFRKIKQFGGHARQGGGVCGVEMALWDLAGKAYNVPVHQMLGGKFRDRIRCYA
DTTESHDPKVYGQRLKARRDEGFTWLKMDLGVDLVADVPGALTMPVGERINGNPMVAHMFTGNEITEKGIQLMCDYVAQI
REIVGMDIPLSADHFGHIGVNSCIRLGKALTKYNMAWLEDMIPWQQTALLKQIADAVDIPILTGEDIYLKEPFIELCKAH
AVDIIHPDLATSGGILETKKIGDAAQELGVAMAMHFAGSPVSCMANVHCAAATENFLVLENHSVDVPWWGDLVEGVSKPI
VSKGFITVPNGPGLGVTLNDEVVKQHLGEPGYFEATPYWNVDRSNDRQWS
>Mature_449_residues
PLDRTRPARRSFLKGTAAALGLGFGVDNVLEALPQNVNTNSRPSELKITDLRVAVVSRAPMTCPIIRIDTNQGIYGLGEV
RDGASKNYALMLKSRLLNENPCNVDKLFRKIKQFGGHARQGGGVCGVEMALWDLAGKAYNVPVHQMLGGKFRDRIRCYAD
TTESHDPKVYGQRLKARRDEGFTWLKMDLGVDLVADVPGALTMPVGERINGNPMVAHMFTGNEITEKGIQLMCDYVAQIR
EIVGMDIPLSADHFGHIGVNSCIRLGKALTKYNMAWLEDMIPWQQTALLKQIADAVDIPILTGEDIYLKEPFIELCKAHA
VDIIHPDLATSGGILETKKIGDAAQELGVAMAMHFAGSPVSCMANVHCAAATENFLVLENHSVDVPWWGDLVEGVSKPIV
SKGFITVPNGPGLGVTLNDEVVKQHLGEPGYFEATPYWNVDRSNDRQWS

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=418, Percent_Identity=24.6411483253589, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787864, Length=398, Percent_Identity=25.8793969849246, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 49329; Mature: 49198

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDRTRPARRSFLKGTAAALGLGFGVDNVLEALPQNVNTNSRPSELKITDLRVAVVSRA
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCC
PMTCPIIRIDTNQGIYGLGEVRDGASKNYALMLKSRLLNENPCNVDKLFRKIKQFGGHAR
CCEEEEEEEECCCCEEECCCCCCCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHCCCCC
QGGGVCGVEMALWDLAGKAYNVPVHQMLGGKFRDRIRCYADTTESHDPKVYGQRLKARRD
CCCCEEHHHHHHHHHCCCEECCCHHHHHCCHHHHHEEEEECCCCCCCCHHHHHHHHHHHC
EGFTWLKMDLGVDLVADVPGALTMPVGERINGNPMVAHMFTGNEITEKGIQLMCDYVAQI
CCCEEEEEECCCEEEECCCCCEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
REIVGMDIPLSADHFGHIGVNSCIRLGKALTKYNMAWLEDMIPWQQTALLKQIADAVDIP
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
ILTGEDIYLKEPFIELCKAHAVDIIHPDLATSGGILETKKIGDAAQELGVAMAMHFAGSP
EECCCCEEECCHHHHHHHHHHHEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
VSCMANVHCAAATENFLVLENHSVDVPWWGDLVEGVSKPIVSKGFITVPNGPGLGVTLND
HHHHHCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCHHCCCEEECCCCCCCEEEECH
EVVKQHLGEPGYFEATPYWNVDRSNDRQWS
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLDRTRPARRSFLKGTAAALGLGFGVDNVLEALPQNVNTNSRPSELKITDLRVAVVSRA
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCC
PMTCPIIRIDTNQGIYGLGEVRDGASKNYALMLKSRLLNENPCNVDKLFRKIKQFGGHAR
CCEEEEEEEECCCCEEECCCCCCCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHCCCCC
QGGGVCGVEMALWDLAGKAYNVPVHQMLGGKFRDRIRCYADTTESHDPKVYGQRLKARRD
CCCCEEHHHHHHHHHCCCEECCCHHHHHCCHHHHHEEEEECCCCCCCCHHHHHHHHHHHC
EGFTWLKMDLGVDLVADVPGALTMPVGERINGNPMVAHMFTGNEITEKGIQLMCDYVAQI
CCCEEEEEECCCEEEECCCCCEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
REIVGMDIPLSADHFGHIGVNSCIRLGKALTKYNMAWLEDMIPWQQTALLKQIADAVDIP
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
ILTGEDIYLKEPFIELCKAHAVDIIHPDLATSGGILETKKIGDAAQELGVAMAMHFAGSP
EECCCCEEECCHHHHHHHHHHHEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
VSCMANVHCAAATENFLVLENHSVDVPWWGDLVEGVSKPIVSKGFITVPNGPGLGVTLND
HHHHHCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCHHCCCEEECCCCCCCEEEECH
EVVKQHLGEPGYFEATPYWNVDRSNDRQWS
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA