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The map label for this gene is mtbC [H]

Identifier: 116621787

GI number: 116621787

Start: 3383085

End: 3383726

Strand: Direct

Name: mtbC [H]

Synonym: Acid_2669

Alternate gene names: 116621787

Gene position: 3383085-3383726 (Clockwise)

Preceding gene: 116621786

Following gene: 116621788

Centisome position: 33.95

GC content: 64.64

Gene sequence:

>642_bases
ATGGTGGATTACGCGCTGATGAACCAATACCTCTACGAGGGGAACGCCGCCGAAGTAGAGAAGATGACGCGCGACGCGTT
GGTGGAGGGCCGCTCCGCGCAGGAGATTCTCAACGAGGGGCTGATCGCGGGCATGAGCGTGGTGGGCGAAGATTTCAAGC
ACAACGTGCTCTATGTGCCCGAGGTCTTGATTGCCGCGCGCGCGATGAAGGCCGGGATGGCGGTGCTCAAGCCGCTGCTC
TCCGCGAAGGAGGGCGGCACCAAACCGGTGGGGGTGCTGCTGATGGGCACGGTGCGCGGCGACCTGCACGATATCGGCAA
GAACCTGGTGCTGATGATGGCGGAGGGCGCGGGCTTCGAGGTCCACGATATCGGCGTGGACCAGAGCCTGGAGAAATTCA
TGGCCGCGGCGGAGCGCTGCAACCCCACCATCATCGGCATGAGCGCGCTGCTCACCACCACGATGACGTATATGAAGACC
GTGATCGATGGCTTCGAGGCCGCCGGCAAGACGCACATCCGGATGGCGGTGGGCGGCGCGCCGATCAGCCAGATGTTCGC
GGACGAAATCGGCGCGGATGGCTATGGGGCCAACGCCTCGGCGGCGGTCGATCTCTTCCTGAAACTCGCGGCGCGGGGCT
GA

Upstream 100 bases:

>100_bases
GCCGCGCGAGAATATACTGGCGATGCTGGAAGCGCTCCAGGAGTTCAACTCCAAGCGTGCGGCCGCCATGGCTGCGAGCG
TCCGGTAACGGGAGACTGGC

Downstream 100 bases:

>100_bases
CCGGAATGGCAACGTACAAGATTCTCTACTGGCAGGAAGTTCCCTCGCAGATCAAGGCGGACGACGGCGTGGATGAAATT
ACTCTCCCCATGCCGCCGCG

Product: cobalamin B12-binding domain-containing protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MVDYALMNQYLYEGNAAEVEKMTRDALVEGRSAQEILNEGLIAGMSVVGEDFKHNVLYVPEVLIAARAMKAGMAVLKPLL
SAKEGGTKPVGVLLMGTVRGDLHDIGKNLVLMMAEGAGFEVHDIGVDQSLEKFMAAAERCNPTIIGMSALLTTTMTYMKT
VIDGFEAAGKTHIRMAVGGAPISQMFADEIGADGYGANASAAVDLFLKLAARG

Sequences:

>Translated_213_residues
MVDYALMNQYLYEGNAAEVEKMTRDALVEGRSAQEILNEGLIAGMSVVGEDFKHNVLYVPEVLIAARAMKAGMAVLKPLL
SAKEGGTKPVGVLLMGTVRGDLHDIGKNLVLMMAEGAGFEVHDIGVDQSLEKFMAAAERCNPTIIGMSALLTTTMTYMKT
VIDGFEAAGKTHIRMAVGGAPISQMFADEIGADGYGANASAAVDLFLKLAARG
>Mature_213_residues
MVDYALMNQYLYEGNAAEVEKMTRDALVEGRSAQEILNEGLIAGMSVVGEDFKHNVLYVPEVLIAARAMKAGMAVLKPLL
SAKEGGTKPVGVLLMGTVRGDLHDIGKNLVLMMAEGAGFEVHDIGVDQSLEKFMAAAERCNPTIIGMSALLTTTMTYMKT
VIDGFEAAGKTHIRMAVGGAPISQMFADEIGADGYGANASAAVDLFLKLAARG

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=176, Percent_Identity=31.25, Blast_Score=74, Evalue=6e-14,
Organism=Escherichia coli, GI1790450, Length=166, Percent_Identity=34.3373493975904, Blast_Score=100, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17535405, Length=160, Percent_Identity=31.875, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 22531; Mature: 22531

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
7.0 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
7.0 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDYALMNQYLYEGNAAEVEKMTRDALVEGRSAQEILNEGLIAGMSVVGEDFKHNVLYVP
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHH
EVLIAARAMKAGMAVLKPLLSAKEGGTKPVGVLLMGTVRGDLHDIGKNLVLMMAEGAGFE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCEEEEEECCCCCE
VHDIGVDQSLEKFMAAAERCNPTIIGMSALLTTTMTYMKTVIDGFEAAGKTHIRMAVGGA
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
PISQMFADEIGADGYGANASAAVDLFLKLAARG
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVDYALMNQYLYEGNAAEVEKMTRDALVEGRSAQEILNEGLIAGMSVVGEDFKHNVLYVP
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHH
EVLIAARAMKAGMAVLKPLLSAKEGGTKPVGVLLMGTVRGDLHDIGKNLVLMMAEGAGFE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCEEEEEECCCCCE
VHDIGVDQSLEKFMAAAERCNPTIIGMSALLTTTMTYMKTVIDGFEAAGKTHIRMAVGGA
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
PISQMFADEIGADGYGANASAAVDLFLKLAARG
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10762254; 10852929 [H]