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The map label for this gene is rhmD [H]

Identifier: 116621779

GI number: 116621779

Start: 3368956

End: 3370224

Strand: Direct

Name: rhmD [H]

Synonym: Acid_2661

Alternate gene names: 116621779

Gene position: 3368956-3370224 (Clockwise)

Preceding gene: 116621775

Following gene: 116621786

Centisome position: 33.81

GC content: 61.78

Gene sequence:

>1269_bases
ATGATGAGCGCAATTTCACGCCGCGGCTTTCTGGCCACGCTGCCCGTGGCTTCGGTTCTGGCACAGACCAAGCCTCTGAA
GATCACCGCCGTGGAGATCTGGCAACTGCACGGGCACCGCGACACGGTGCGCGGGATCGATCAGCAGTACCAGGCCAATC
CGCTGTTCATCTATGACGAATTGCGGCCGAAGCCGTATGCCGATGCCGCGCAGGGCACGACGCAGAACTCAGCCGTGAGC
GCGCTGTACCTCAAGATTAAGACGGATGGCGGCGTGGACGGGCTCTACGGGCCGATCGACAACGAGGTCGCGATCGTAGT
GGACCAGCAGCTACGTGCCTTCCTGATGGGGAAGGACGCGCTGGCGCAGGAGAAGTTGTGGGACCAGCTCTACCGCTCTA
ACCGGCACGCGCGCCGCGGCGCTTTCCTGATGGCGATCAGCGCCGTGGATAACACTCTGTGGGATCTGCGAGGACGCTTT
TTCAAGACGCCGGTTTACCGGCTGCTGGGCGGTCCGACGCGCGCTTCGATCGAGGCGTACGGGAGTTGCCTTGGATACTC
GCTGGAGCCCGACAAGGCACAGTCGCGCGCGGTTCAACTGAAGAAGGAAGGCTATCGCTACCAGAAGTGGTTCCTGGCTT
ATGGACCCGGCGACGGTCCCGAAGGCTTGAAGAAGAACGTAGACCTGGTGCGGGTGTTGCGCGAAGCGGTCGGCGACGAG
ACGGAGCTGATGTTCGACGTGTACAGCGGCTGGGATCTCAGCTACGCGCTGAGTTGGGCGCGGCAGGTGGAGCGCTATCG
TCCGCGCTGGATCGAGGAGGCGACACAGGCCGAGAAGATCGACAGCTTTGCGGCGTTACGGAAGGGGACGTCAATTCCGA
TTGCGTCGGGCGAACACATTCAGGGGCGGTGGGAGGTCTACAACTATCTGAAGGAAGGCGCGCTGAGCGTGGTGCAGTGT
GACCCTGAATGGTGCGGGGGGACGACGGAGCTGCAGAAGATTTGCGTGCTGGCGTCGATTTTCGATGTGCCGGTGATTCC
GCACGGGCATAGCCTGCACGCCGCGCTGCATTTGATCGCGAGCCAGTCGCCCGCGGTGTGTCCGCTGGCGGAGTACCTGA
TTTTGAAGATGCGATCGTACTACCACTTCGAGAAGAATCCGCCGGCGCCGAAGAATGCGCATTTCGATTTGCCGGCGGGG
CCGGGGTATGGGATCGAGTTCGATGAAGGGAAGGTGGAGAAGCAGGAATTGATGAAGTACGCCGGCTGA

Upstream 100 bases:

>100_bases
TGGTCTGGCCGATTAAGGCCGGGGAGGCGGCAAGCGCCAGGAAAATTCCGGACAATCGCATAGTCGATTCCAGTCAGTTT
AGTTCTAGAATCAAACTAAC

Downstream 100 bases:

>100_bases
CAGGGCAAGTCCCAGAGGGGAAGGCGGCTGGCGGGTGCCGGGCGTGAGCGGAGAAGCCGCCTGAAAGGCGGCTGCAGCCA
GGATTGGCTGCCCCATAAGG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MMSAISRRGFLATLPVASVLAQTKPLKITAVEIWQLHGHRDTVRGIDQQYQANPLFIYDELRPKPYADAAQGTTQNSAVS
ALYLKIKTDGGVDGLYGPIDNEVAIVVDQQLRAFLMGKDALAQEKLWDQLYRSNRHARRGAFLMAISAVDNTLWDLRGRF
FKTPVYRLLGGPTRASIEAYGSCLGYSLEPDKAQSRAVQLKKEGYRYQKWFLAYGPGDGPEGLKKNVDLVRVLREAVGDE
TELMFDVYSGWDLSYALSWARQVERYRPRWIEEATQAEKIDSFAALRKGTSIPIASGEHIQGRWEVYNYLKEGALSVVQC
DPEWCGGTTELQKICVLASIFDVPVIPHGHSLHAALHLIASQSPAVCPLAEYLILKMRSYYHFEKNPPAPKNAHFDLPAG
PGYGIEFDEGKVEKQELMKYAG

Sequences:

>Translated_422_residues
MMSAISRRGFLATLPVASVLAQTKPLKITAVEIWQLHGHRDTVRGIDQQYQANPLFIYDELRPKPYADAAQGTTQNSAVS
ALYLKIKTDGGVDGLYGPIDNEVAIVVDQQLRAFLMGKDALAQEKLWDQLYRSNRHARRGAFLMAISAVDNTLWDLRGRF
FKTPVYRLLGGPTRASIEAYGSCLGYSLEPDKAQSRAVQLKKEGYRYQKWFLAYGPGDGPEGLKKNVDLVRVLREAVGDE
TELMFDVYSGWDLSYALSWARQVERYRPRWIEEATQAEKIDSFAALRKGTSIPIASGEHIQGRWEVYNYLKEGALSVVQC
DPEWCGGTTELQKICVLASIFDVPVIPHGHSLHAALHLIASQSPAVCPLAEYLILKMRSYYHFEKNPPAPKNAHFDLPAG
PGYGIEFDEGKVEKQELMKYAG
>Mature_422_residues
MMSAISRRGFLATLPVASVLAQTKPLKITAVEIWQLHGHRDTVRGIDQQYQANPLFIYDELRPKPYADAAQGTTQNSAVS
ALYLKIKTDGGVDGLYGPIDNEVAIVVDQQLRAFLMGKDALAQEKLWDQLYRSNRHARRGAFLMAISAVDNTLWDLRGRF
FKTPVYRLLGGPTRASIEAYGSCLGYSLEPDKAQSRAVQLKKEGYRYQKWFLAYGPGDGPEGLKKNVDLVRVLREAVGDE
TELMFDVYSGWDLSYALSWARQVERYRPRWIEEATQAEKIDSFAALRKGTSIPIASGEHIQGRWEVYNYLKEGALSVVQC
DPEWCGGTTELQKICVLASIFDVPVIPHGHSLHAALHLIASQSPAVCPLAEYLILKMRSYYHFEKNPPAPKNAHFDLPAG
PGYGIEFDEGKVEKQELMKYAG

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=404, Percent_Identity=26.2376237623762, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI186972148, Length=330, Percent_Identity=26.6666666666667, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI42544117, Length=440, Percent_Identity=24.0909090909091, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI226510960, Length=345, Percent_Identity=28.4057971014493, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI48994953, Length=345, Percent_Identity=31.304347826087, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787864, Length=363, Percent_Identity=29.7520661157025, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 47274; Mature: 47274

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSAISRRGFLATLPVASVLAQTKPLKITAVEIWQLHGHRDTVRGIDQQYQANPLFIYDE
CCCCHHCCCCEEHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHCCHHHHCCCCEEEEEC
LRPKPYADAAQGTTQNSAVSALYLKIKTDGGVDGLYGPIDNEVAIVVDQQLRAFLMGKDA
CCCCCCCCHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCHH
LAQEKLWDQLYRSNRHARRGAFLMAISAVDNTLWDLRGRFFKTPVYRLLGGPTRASIEAY
HHHHHHHHHHHHCCCCHHCCEEEEEEHHHHHHHHHHCCHHHHCHHHHHHCCCCHHHHHHH
GSCLGYSLEPDKAQSRAVQLKKEGYRYQKWFLAYGPGDGPEGLKKNVDLVRVLREAVGDE
HHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHCCHHHHHHHHHHHCCC
TELMFDVYSGWDLSYALSWARQVERYRPRWIEEATQAEKIDSFAALRKGTSIPIASGEHI
CEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QGRWEVYNYLKEGALSVVQCDPEWCGGTTELQKICVLASIFDVPVIPHGHSLHAALHLIA
CHHHHHHHHHHHCCCEEEEECHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SQSPAVCPLAEYLILKMRSYYHFEKNPPAPKNAHFDLPAGPGYGIEFDEGKVEKQELMKY
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHH
AG
CC
>Mature Secondary Structure
MMSAISRRGFLATLPVASVLAQTKPLKITAVEIWQLHGHRDTVRGIDQQYQANPLFIYDE
CCCCHHCCCCEEHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHCCHHHHCCCCEEEEEC
LRPKPYADAAQGTTQNSAVSALYLKIKTDGGVDGLYGPIDNEVAIVVDQQLRAFLMGKDA
CCCCCCCCHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCHH
LAQEKLWDQLYRSNRHARRGAFLMAISAVDNTLWDLRGRFFKTPVYRLLGGPTRASIEAY
HHHHHHHHHHHHCCCCHHCCEEEEEEHHHHHHHHHHCCHHHHCHHHHHHCCCCHHHHHHH
GSCLGYSLEPDKAQSRAVQLKKEGYRYQKWFLAYGPGDGPEGLKKNVDLVRVLREAVGDE
HHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHCCHHHHHHHHHHHCCC
TELMFDVYSGWDLSYALSWARQVERYRPRWIEEATQAEKIDSFAALRKGTSIPIASGEHI
CEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QGRWEVYNYLKEGALSVVQCDPEWCGGTTELQKICVLASIFDVPVIPHGHSLHAALHLIA
CHHHHHHHHHHHCCCEEEEECHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SQSPAVCPLAEYLILKMRSYYHFEKNPPAPKNAHFDLPAGPGYGIEFDEGKVEKQELMKY
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHH
AG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA