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The map label for this gene is emrB [H]

Identifier: 116621740

GI number: 116621740

Start: 3314053

End: 3315630

Strand: Reverse

Name: emrB [H]

Synonym: Acid_2622

Alternate gene names: 116621740

Gene position: 3315630-3314053 (Counterclockwise)

Preceding gene: 116621741

Following gene: 116621734

Centisome position: 33.27

GC content: 61.98

Gene sequence:

>1578_bases
ATGCCGATTGACGAATCCCAGGGCTGGCATCCCGGCCACAGTCCCTGGACGGTTGCCTTGACCGTCACGATCGCCACGTT
CATGGAGGTGATGGATACCTCCATCGCGAACGTGTCGCTGCCGCACATCGCCGGCGGACTGTCGGCCGGGGTCGATGAAT
CCACCTGGATTCTTACCAGTTATCTGGTCTCCAACGCGATCGTGCTGCCCATCTCCGCCTGGCTGAGCAACCGCTTCGGC
CGCAAACGCTTCTACATGACTTCGGTCGCGATCTTCACGGTCAGTTCCCTGCTCTGCGGACTGGCGCCAAGCCTGTCGCT
GCTCATCTTCTTCCGAATCCTGCAGGGTGTCGGCGGCGGCGGCCTCGGCCCCAGCGAGCAGGCCATTCTCACCGATACTT
TTCCCGCCGCCAAGCGCGGCATGGCCTTCGCCGTTTATGGCATGGCTGTCGTGCTCGCCCCTGCCATCGGTCCCACGCTG
GGCGGCTGGATCACCGACCAGTACAGTTGGCGCTGGATCTTCTTCATTAACGTCCCGTTCGGCATTCTCTCGCTCTTCCT
CACCAATCATATGGTGCAGGATCCGCCGGATATGGAAAGAGCCAAGCAGGATGCCAAAAAGGCGCCTGTCGATTTCCTCG
GCCTCGTGCTGATCGCCACCGGACTCGGCGCATTCCAGATCATGCTCGACAAAGGCCAGCGCGACGACTGGTTCGCCTCG
CCGTTCATCACCGCGTGCGTCATTGTCACCGTCGGCGCGCTTGGCGCTTTCGTGATCTGGGAGTGGAACCACCGCCACCC
CATCGTGGAGCTCGGCCTTTTGAAGAACCGCAGTTTCGCGGTCGCCTGCATGCTCATGCTGGTCACCTTCGCGGTCATGC
TCGGCAGCACCTTGCTGATTCCTCAGTTCGCGCAGCTTGAACTCGGCTACACCGCGCGCCAGGCCGGTGAGTTGCTCTCA
CCCGGCGGCTTTACCATCCTGATTCTGATGCCCATGATCGGCTACCTGGTCTCGCGCGTCGATGCGCGTTATCTCATCGG
CATTGGATACCTGATCTTCGCCGCCTCTCTCTTCCACATGACCAACCTCTACCTCGGCGTGGATTTCCGCACCGCCGTGA
TGTGGCGCGTCTACCAAGCCGCCGGCATGGCCTTCCTGTTCGTCCCCACCAACACCATCGCCTATACCGATATGACGCCG
GGCGCCAGCAACCAGGTGTCCGCCCTCACCAATCTGATGCGCAACATGGGAGGCAGCGTCGGTATCTCCGCGGTCACCAC
ACTGCTCGCGCGCCGGCAGCAGACCCACCAGGTCTTCCTGGTCCGCAACGTCACCTCAGCCGACCCGCACTTAAAGCAGA
TGCTGACCCAGTTCGCCAATCGCTTCAGCCTCCGCACGGATTCCCATCACGCCATGCAGCAGGCGTACGGCGCGATCTAT
CGCCAGGTTCAAAGCCAAGCCGCGGTGCTCGCCTACATCGACGTGATCACCATCATGGGCGCGCTCTGCCTGGCCGCCAT
CCTGCTCTTGTTCTTCGCCAAAAAGTCCAAACCCGGCCAGGCCGCCGTGGCGCACTAA

Upstream 100 bases:

>100_bases
CGGCTGCCAGTGCGCATCCGCTTCAAGCAGGGCCAAAACGGGCTCGACCGCCTCCGTCCCGGCATGTCCGTCGAACCCAA
AGTGAGGGTCCGGTAGCCTC

Downstream 100 bases:

>100_bases
CTCCTACCCGCTCCCGCTTTGCTGAAGGCTCCAAACATAACCCTGCGGGGCCAGCCGCTACGCATGAAGGTGCGATCTCA
AACAAACGAGCCGCGTCCGT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MPIDESQGWHPGHSPWTVALTVTIATFMEVMDTSIANVSLPHIAGGLSAGVDESTWILTSYLVSNAIVLPISAWLSNRFG
RKRFYMTSVAIFTVSSLLCGLAPSLSLLIFFRILQGVGGGGLGPSEQAILTDTFPAAKRGMAFAVYGMAVVLAPAIGPTL
GGWITDQYSWRWIFFINVPFGILSLFLTNHMVQDPPDMERAKQDAKKAPVDFLGLVLIATGLGAFQIMLDKGQRDDWFAS
PFITACVIVTVGALGAFVIWEWNHRHPIVELGLLKNRSFAVACMLMLVTFAVMLGSTLLIPQFAQLELGYTARQAGELLS
PGGFTILILMPMIGYLVSRVDARYLIGIGYLIFAASLFHMTNLYLGVDFRTAVMWRVYQAAGMAFLFVPTNTIAYTDMTP
GASNQVSALTNLMRNMGGSVGISAVTTLLARRQQTHQVFLVRNVTSADPHLKQMLTQFANRFSLRTDSHHAMQQAYGAIY
RQVQSQAAVLAYIDVITIMGALCLAAILLLFFAKKSKPGQAAVAH

Sequences:

>Translated_525_residues
MPIDESQGWHPGHSPWTVALTVTIATFMEVMDTSIANVSLPHIAGGLSAGVDESTWILTSYLVSNAIVLPISAWLSNRFG
RKRFYMTSVAIFTVSSLLCGLAPSLSLLIFFRILQGVGGGGLGPSEQAILTDTFPAAKRGMAFAVYGMAVVLAPAIGPTL
GGWITDQYSWRWIFFINVPFGILSLFLTNHMVQDPPDMERAKQDAKKAPVDFLGLVLIATGLGAFQIMLDKGQRDDWFAS
PFITACVIVTVGALGAFVIWEWNHRHPIVELGLLKNRSFAVACMLMLVTFAVMLGSTLLIPQFAQLELGYTARQAGELLS
PGGFTILILMPMIGYLVSRVDARYLIGIGYLIFAASLFHMTNLYLGVDFRTAVMWRVYQAAGMAFLFVPTNTIAYTDMTP
GASNQVSALTNLMRNMGGSVGISAVTTLLARRQQTHQVFLVRNVTSADPHLKQMLTQFANRFSLRTDSHHAMQQAYGAIY
RQVQSQAAVLAYIDVITIMGALCLAAILLLFFAKKSKPGQAAVAH
>Mature_524_residues
PIDESQGWHPGHSPWTVALTVTIATFMEVMDTSIANVSLPHIAGGLSAGVDESTWILTSYLVSNAIVLPISAWLSNRFGR
KRFYMTSVAIFTVSSLLCGLAPSLSLLIFFRILQGVGGGGLGPSEQAILTDTFPAAKRGMAFAVYGMAVVLAPAIGPTLG
GWITDQYSWRWIFFINVPFGILSLFLTNHMVQDPPDMERAKQDAKKAPVDFLGLVLIATGLGAFQIMLDKGQRDDWFASP
FITACVIVTVGALGAFVIWEWNHRHPIVELGLLKNRSFAVACMLMLVTFAVMLGSTLLIPQFAQLELGYTARQAGELLSP
GGFTILILMPMIGYLVSRVDARYLIGIGYLIFAASLFHMTNLYLGVDFRTAVMWRVYQAAGMAFLFVPTNTIAYTDMTPG
ASNQVSALTNLMRNMGGSVGISAVTTLLARRQQTHQVFLVRNVTSADPHLKQMLTQFANRFSLRTDSHHAMQQAYGAIYR
QVQSQAAVLAYIDVITIMGALCLAAILLLFFAKKSKPGQAAVAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=499, Percent_Identity=34.2685370741483, Blast_Score=324, Evalue=8e-90,
Organism=Escherichia coli, GI1788710, Length=486, Percent_Identity=33.9506172839506, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI87081983, Length=433, Percent_Identity=29.3302540415704, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1790195, Length=346, Percent_Identity=30.635838150289, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1788392, Length=432, Percent_Identity=26.3888888888889, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1790146, Length=170, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=284, Percent_Identity=29.2253521126761, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321663, Length=167, Percent_Identity=29.940119760479, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=126, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6322958, Length=263, Percent_Identity=25.4752851711027, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323735, Length=302, Percent_Identity=24.1721854304636, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57189; Mature: 57058

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIDESQGWHPGHSPWTVALTVTIATFMEVMDTSIANVSLPHIAGGLSAGVDESTWILTS
CCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHH
YLVSNAIVLPISAWLSNRFGRKRFYMTSVAIFTVSSLLCGLAPSLSLLIFFRILQGVGGG
HHHHCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
GLGPSEQAILTDTFPAAKRGMAFAVYGMAVVLAPAIGPTLGGWITDQYSWRWIFFINVPF
CCCCCCCEEEECCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCH
GILSLFLTNHMVQDPPDMERAKQDAKKAPVDFLGLVLIATGLGAFQIMLDKGQRDDWFAS
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
PFITACVIVTVGALGAFVIWEWNHRHPIVELGLLKNRSFAVACMLMLVTFAVMLGSTLLI
HHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHC
PQFAQLELGYTARQAGELLSPGGFTILILMPMIGYLVSRVDARYLIGIGYLIFAASLFHM
CCHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
TNLYLGVDFRTAVMWRVYQAAGMAFLFVPTNTIAYTDMTPGASNQVSALTNLMRNMGGSV
HHHHCCCCHHHHHHHHHHHHCCEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHCCCCC
GISAVTTLLARRQQTHQVFLVRNVTSADPHLKQMLTQFANRFSLRTDSHHAMQQAYGAIY
CHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RQVQSQAAVLAYIDVITIMGALCLAAILLLFFAKKSKPGQAAVAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PIDESQGWHPGHSPWTVALTVTIATFMEVMDTSIANVSLPHIAGGLSAGVDESTWILTS
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHH
YLVSNAIVLPISAWLSNRFGRKRFYMTSVAIFTVSSLLCGLAPSLSLLIFFRILQGVGGG
HHHHCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
GLGPSEQAILTDTFPAAKRGMAFAVYGMAVVLAPAIGPTLGGWITDQYSWRWIFFINVPF
CCCCCCCEEEECCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCH
GILSLFLTNHMVQDPPDMERAKQDAKKAPVDFLGLVLIATGLGAFQIMLDKGQRDDWFAS
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
PFITACVIVTVGALGAFVIWEWNHRHPIVELGLLKNRSFAVACMLMLVTFAVMLGSTLLI
HHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHC
PQFAQLELGYTARQAGELLSPGGFTILILMPMIGYLVSRVDARYLIGIGYLIFAASLFHM
CCHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
TNLYLGVDFRTAVMWRVYQAAGMAFLFVPTNTIAYTDMTPGASNQVSALTNLMRNMGGSV
HHHHCCCCHHHHHHHHHHHHCCEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHCCCCC
GISAVTTLLARRQQTHQVFLVRNVTSADPHLKQMLTQFANRFSLRTDSHHAMQQAYGAIY
CHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RQVQSQAAVLAYIDVITIMGALCLAAILLLFFAKKSKPGQAAVAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]