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The map label for this gene is gltS [H]

Identifier: 116621677

GI number: 116621677

Start: 3240060

End: 3241235

Strand: Reverse

Name: gltS [H]

Synonym: Acid_2559

Alternate gene names: 116621677

Gene position: 3241235-3240060 (Counterclockwise)

Preceding gene: 116621679

Following gene: 116621667

Centisome position: 32.52

GC content: 65.05

Gene sequence:

>1176_bases
ATGATCCCCACCGTCAAAATCAGCGCCGTACAAGTGCTGGGCCTGGCCGCTCTCGGCGTGGTCATGGGCGCGTGGCTCAA
ACGCCGCGTCCCCCTGCTCGACCAGCTCAACATCCCTGTCTCGATTCTAGGCGGCATGATATACGCCATCGCGGCGCTGG
CCTGCCGCGATCGCCTGGTCAACTTCGACGCCGACGTGGTGCTGCGCGACCTGCTGATGATCGCATTCATGACCACCATC
GGCCTGAGCGCCCGCCTCGAACTGGTCCGTCGCGGAGGCGCGCGAATGCTCTGGTTCCTGGGCGCCGCCACCTTGGGTGC
CGTGCTGCAGAATTTTCTCGGAATGGGGCTCGCGCACCTGCTCGGTGTGGACCCGCGCCTCGGCATTCTCACCGGCTCGG
TCTCGCTCGCCGGCGGACCCGCCACCGCCTTGGCCTTCGGCGGCACCTTCGAGAAGATGGGCGTGCCCGGAGCCACCACG
GTCGCCTTCGCCTCTGCGACTTTCGGGATCACGGTCGCCGGATTGATCGGAGGGTACATCGGCGGCCGCCTGATCCGTCG
GCACAAGCTGCAACCCGCCGCCGGAACGGCCGCCCGCGAAGTTCGCACCGATGGCAGCGCGGGATCGTTGATGACCGTGG
TCCTGGTGCTCGGCATCGCCATGGGCGCGGGCAGCCTGGTGAGCGCGGGAATTCAATCGCTCGGCGTGATTCTGCCGGTC
TACATCGGCAGCATGATTGTCGCCGCCGTGCTGCGCAATCTCAACGATCATTTCCGCTTCGCGGAAATAGCGCAGCCCGA
AGTAGACATGGTTGGGCGCCTCGCGCTCTATCTCTTCATCGTGATGGCGCTGATCACGCTGCGCCTCTGGGAACTCGCGC
ACCTTGCGCTGCCGATGTTCGCCATGCTGGCCGTGCAGGTGGTCTTCTGCTGCGCGATGTGCGTCACGCTCTCCTGGTGG
GTGATGGGCCGCAATTACGAAGCCGCGGTCAGCTCCGCCGGTTTCTGCGGCTTCATGCTCGGCATCACTGCGAACGCCGT
CGCCTGCATGGAGGAGTTGGTCGAGAAGTACGGCCCCGCGCCGCAGGCATTCCTGATCGTCCCGATCGTCGGCGCTTTCC
TGATAGACTTCACGAACTCCCTGATCATCACCGCCTTCGCCAACTGGCTGAAGTAA

Upstream 100 bases:

>100_bases
TAGTTATCCGCGAAGGCGAGCGAACAAGACAGCGCGAGGCCGAACCGGAGCAGTTTCTTCATCGACCCTCTTTCGAGCTA
CTATACTGCAATTTGGCCGC

Downstream 100 bases:

>100_bases
GTGCAAACCACGATTTCAGTCTGCGGCTTGCCGCACTTGTTTGCTCGAAGGCCGCAAACCTCCGCACCGAAATATTCGCC
GCCCCATCAGAGCGATCGCT

Product: sodium/glutamate symporter

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MIPTVKISAVQVLGLAALGVVMGAWLKRRVPLLDQLNIPVSILGGMIYAIAALACRDRLVNFDADVVLRDLLMIAFMTTI
GLSARLELVRRGGARMLWFLGAATLGAVLQNFLGMGLAHLLGVDPRLGILTGSVSLAGGPATALAFGGTFEKMGVPGATT
VAFASATFGITVAGLIGGYIGGRLIRRHKLQPAAGTAAREVRTDGSAGSLMTVVLVLGIAMGAGSLVSAGIQSLGVILPV
YIGSMIVAAVLRNLNDHFRFAEIAQPEVDMVGRLALYLFIVMALITLRLWELAHLALPMFAMLAVQVVFCCAMCVTLSWW
VMGRNYEAAVSSAGFCGFMLGITANAVACMEELVEKYGPAPQAFLIVPIVGAFLIDFTNSLIITAFANWLK

Sequences:

>Translated_391_residues
MIPTVKISAVQVLGLAALGVVMGAWLKRRVPLLDQLNIPVSILGGMIYAIAALACRDRLVNFDADVVLRDLLMIAFMTTI
GLSARLELVRRGGARMLWFLGAATLGAVLQNFLGMGLAHLLGVDPRLGILTGSVSLAGGPATALAFGGTFEKMGVPGATT
VAFASATFGITVAGLIGGYIGGRLIRRHKLQPAAGTAAREVRTDGSAGSLMTVVLVLGIAMGAGSLVSAGIQSLGVILPV
YIGSMIVAAVLRNLNDHFRFAEIAQPEVDMVGRLALYLFIVMALITLRLWELAHLALPMFAMLAVQVVFCCAMCVTLSWW
VMGRNYEAAVSSAGFCGFMLGITANAVACMEELVEKYGPAPQAFLIVPIVGAFLIDFTNSLIITAFANWLK
>Mature_391_residues
MIPTVKISAVQVLGLAALGVVMGAWLKRRVPLLDQLNIPVSILGGMIYAIAALACRDRLVNFDADVVLRDLLMIAFMTTI
GLSARLELVRRGGARMLWFLGAATLGAVLQNFLGMGLAHLLGVDPRLGILTGSVSLAGGPATALAFGGTFEKMGVPGATT
VAFASATFGITVAGLIGGYIGGRLIRRHKLQPAAGTAAREVRTDGSAGSLMTVVLVLGIAMGAGSLVSAGIQSLGVILPV
YIGSMIVAAVLRNLNDHFRFAEIAQPEVDMVGRLALYLFIVMALITLRLWELAHLALPMFAMLAVQVVFCCAMCVTLSWW
VMGRNYEAAVSSAGFCGFMLGITANAVACMEELVEKYGPAPQAFLIVPIVGAFLIDFTNSLIITAFANWLK

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=337, Percent_Identity=40.0593471810089, Blast_Score=206, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 41156; Mature: 41156

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]