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The map label for this gene is argF [H]

Identifier: 116621623

GI number: 116621623

Start: 3179004

End: 3179981

Strand: Reverse

Name: argF [H]

Synonym: Acid_2505

Alternate gene names: 116621623

Gene position: 3179981-3179004 (Counterclockwise)

Preceding gene: 116621624

Following gene: 116621622

Centisome position: 31.91

GC content: 63.29

Gene sequence:

>978_bases
GTGAATAATAATTCAGAGACGCACGCCGTGGCCCCCATGTTTGCTCCGAACCAGATTCTCAAAACCAGCGCGTCCGACCT
GTTAAAGGATCTCGATCTCACCGACGATGAGCTCCTTTACCTCCTCGACCTCGCCGCCGAAGTCAAAGCTTCCCCGCGTG
ACTACGCCCGCGCCCTCGATGGCAAGAATCTCGGCCTCCTCTTCGAAAAACCCTCCCTCCGCACCAAGCTCACCTTCGAG
CTCGCCATGAAGCAGATGGGCGGCGGCTCCGTCTTCGTCGAAGGCCCGATCGGCGTCCGCGAACCGCTCAAGGACATCGC
CCGCAATCTCGATCGCTGGGTGGATGGCATCGTCGCCCGCGTCTTCCTCCAGAGCACCGTCGATGGCCTCGCCGAATGGT
CGCGCGTCCCCGTTATCAACGCCCTCAGCGATCTCTATCATCCCTGCCAGGCCCTCGCGGACATGCAGACCATCCGCGAG
CACTTCCGTGTCGCGCGCCTGCACGGCCTCAAGCTGGCCTTCATCGGCGACGGCAATAACGTCGCCCAGTCGCTCATGCT
CACCTCGCTCCGCCTCGGCATGGATTTCGCGCTCGGCTGCCCCGAAGGCTACCTGCCCGATCCCGAAATCGTCACCCAGG
CCGAAGGCCTCGCCGCCGTCACCGGCGCCTCTCTGTTGATCACGCACGATACCGGCGCCGCCGTCGAAGGCGCCCACACC
GTCTATACCGACGTCTGGGCCAGCATGGGCCAGGAACAGGAAACCATCGAGCGCCGCCGCGCCTTCCGCGAGTACCAGGT
CAATGACGTCCTCTTCGCCCAAGCCCGTCCCGACGCAGTCTTCATGCACTGCCTCCCCGCCAAACGCGATGAAGAGGTGA
CCGATTCCATCATGGAACATCCGCGCTCGGTGATCTTCCACCAGGCGGAAAACCGGCTGCACGCCCAAAAGGCCCTGCTG
CTGATGATCCTGACGTAG

Upstream 100 bases:

>100_bases
AAAACTTAGAATCTATGCTTGCAATCTAGCGGAGAGCGAACACCCACGTGCGCCTGATTCTTCTTGACCTTATCCCCAGA
ATGTGTGATTATTCATTAAG

Downstream 100 bases:

>100_bases
AGTATTGGTTGGACTCACCCCTGACGTGAGCGCCCGACAGCGAACCGCGACCGTAAAGGAACCGATCTAAGATTATGACT
ATTCCGAACTACGCCCGTGC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MNNNSETHAVAPMFAPNQILKTSASDLLKDLDLTDDELLYLLDLAAEVKASPRDYARALDGKNLGLLFEKPSLRTKLTFE
LAMKQMGGGSVFVEGPIGVREPLKDIARNLDRWVDGIVARVFLQSTVDGLAEWSRVPVINALSDLYHPCQALADMQTIRE
HFRVARLHGLKLAFIGDGNNVAQSLMLTSLRLGMDFALGCPEGYLPDPEIVTQAEGLAAVTGASLLITHDTGAAVEGAHT
VYTDVWASMGQEQETIERRRAFREYQVNDVLFAQARPDAVFMHCLPAKRDEEVTDSIMEHPRSVIFHQAENRLHAQKALL
LMILT

Sequences:

>Translated_325_residues
MNNNSETHAVAPMFAPNQILKTSASDLLKDLDLTDDELLYLLDLAAEVKASPRDYARALDGKNLGLLFEKPSLRTKLTFE
LAMKQMGGGSVFVEGPIGVREPLKDIARNLDRWVDGIVARVFLQSTVDGLAEWSRVPVINALSDLYHPCQALADMQTIRE
HFRVARLHGLKLAFIGDGNNVAQSLMLTSLRLGMDFALGCPEGYLPDPEIVTQAEGLAAVTGASLLITHDTGAAVEGAHT
VYTDVWASMGQEQETIERRRAFREYQVNDVLFAQARPDAVFMHCLPAKRDEEVTDSIMEHPRSVIFHQAENRLHAQKALL
LMILT
>Mature_325_residues
MNNNSETHAVAPMFAPNQILKTSASDLLKDLDLTDDELLYLLDLAAEVKASPRDYARALDGKNLGLLFEKPSLRTKLTFE
LAMKQMGGGSVFVEGPIGVREPLKDIARNLDRWVDGIVARVFLQSTVDGLAEWSRVPVINALSDLYHPCQALADMQTIRE
HFRVARLHGLKLAFIGDGNNVAQSLMLTSLRLGMDFALGCPEGYLPDPEIVTQAEGLAAVTGASLLITHDTGAAVEGAHT
VYTDVWASMGQEQETIERRRAFREYQVNDVLFAQARPDAVFMHCLPAKRDEEVTDSIMEHPRSVIFHQAENRLHAQKALL
LMILT

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=306, Percent_Identity=41.5032679738562, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=39.1437308868502, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1786469, Length=325, Percent_Identity=37.5384615384615, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI48994908, Length=364, Percent_Identity=25.2747252747253, Blast_Score=86, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322373, Length=312, Percent_Identity=32.6923076923077, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322331, Length=283, Percent_Identity=28.9752650176678, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24642586, Length=278, Percent_Identity=27.6978417266187, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 35961; Mature: 35961

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNSETHAVAPMFAPNQILKTSASDLLKDLDLTDDELLYLLDLAAEVKASPRDYARALD
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHC
GKNLGLLFEKPSLRTKLTFELAMKQMGGGSVFVEGPIGVREPLKDIARNLDRWVDGIVAR
CCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
VFLQSTVDGLAEWSRVPVINALSDLYHPCQALADMQTIREHFRVARLHGLKLAFIGDGNN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHH
VAQSLMLTSLRLGMDFALGCPEGYLPDPEIVTQAEGLAAVTGASLLITHDTGAAVEGAHT
HHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHCCCCCEECCCEEEEEECCCCCCCCCHH
VYTDVWASMGQEQETIERRRAFREYQVNDVLFAQARPDAVFMHCLPAKRDEEVTDSIMEH
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHC
PRSVIFHQAENRLHAQKALLLMILT
HHHHHHHHHHHHHHHHHHHEEEEEC
>Mature Secondary Structure
MNNNSETHAVAPMFAPNQILKTSASDLLKDLDLTDDELLYLLDLAAEVKASPRDYARALD
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHC
GKNLGLLFEKPSLRTKLTFELAMKQMGGGSVFVEGPIGVREPLKDIARNLDRWVDGIVAR
CCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
VFLQSTVDGLAEWSRVPVINALSDLYHPCQALADMQTIREHFRVARLHGLKLAFIGDGNN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHH
VAQSLMLTSLRLGMDFALGCPEGYLPDPEIVTQAEGLAAVTGASLLITHDTGAAVEGAHT
HHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHCCCCCEECCCEEEEEECCCCCCCCCHH
VYTDVWASMGQEQETIERRRAFREYQVNDVLFAQARPDAVFMHCLPAKRDEEVTDSIMEH
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHC
PRSVIFHQAENRLHAQKALLLMILT
HHHHHHHHHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA