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The map label for this gene is est [H]

Identifier: 116621249

GI number: 116621249

Start: 2729836

End: 2730705

Strand: Direct

Name: est [H]

Synonym: Acid_2130

Alternate gene names: 116621249

Gene position: 2729836-2730705 (Clockwise)

Preceding gene: 116621248

Following gene: 116621253

Centisome position: 27.39

GC content: 68.85

Gene sequence:

>870_bases
ATGCCGCTCGATCCTGAAGTCGCCGCCCACCTCGAGCTCCAGCGTCACCTACCGCCGCGCTCCTCGCTCGATATCGCCGC
CACCCGCGAGATGATGCGCCGCGCTGCCGCCCTCTCCGGCCCTCCTCCGTTGCTCCCACGCGTCGAAGATATCGCGCTCC
CCACCGGGCTCCGCGCGCGACAGTATTGGCCCGCGCTCGACCCCGCCCTCCCCCTGACTGTTTACTTCCACGGCGGCCGC
TTCTTCAGCGGCGATCTCGAAAGCCACGACACCATTTGCCGCCTCCTCGCCCTGGCCTCTGCCTCCCGCGTCCTCGCCGT
CGATTACCGTCTCGCCCCCGAGCATCCTTTCCCCGCCGCTGTCGAAGACGCGCGCACCGCCCTCGCCTGGGCCCTCCTTC
AAGACGTCCCCGTCGCCGTCGCTGGCGATAGCGCCGGCGCCAATCTCGCCGCTGTCGCCGCCCTCGACCATCGCCACTCC
CTGCGACTCCGCTGCCAGTTACTCATCTATCCCATGATCGACGCCGTCTGCGCGTCGCACTCTTATACCGAGTTCGCTGA
AGGCTACGGCCCCGGTGCCGCCGACATGCGCCGCGGCTGGTCCGAATATCTTCCCGCGGGCTCCGATCCCCGCGATCCGC
TCGCTTCGCCGCTCTACACTCCCGACCTCACCGGCGCCGCCCCGGCGCTTGTGATCACCGCCGAATACGACACCCTTCGC
GACGAGGCCGAATCCTACGCCCGTCGCCTATCCGCCCCAGTCCAACGCTATCCGGGCGCCATCCACGGCTTCTTCCTAAT
GCAATCGCCCCTCGCCCTCGCCCGTCAGTCCCTTCAGGACGCCGCCGCCTTCCTCCGCCATCACCTGTGA

Upstream 100 bases:

>100_bases
GATCAGACCGTCCGCGATGGCCGCGTCGTCACCGCCCAAACCTGGCAGTCGCACCCGTCCTTCTATCGCGAAATCTTCGC
GCTCCTGCGCGCCAAAGCCG

Downstream 100 bases:

>100_bases
AAACGGCTGCCCGAATGGTGTCGCCGGCTCGCCCTACATCCCTTCCACCGCCGAAGTTCGCGGCCAGGGTGGGATCTGCC
CTGCTAGACGCCGCCGAACG

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MPLDPEVAAHLELQRHLPPRSSLDIAATREMMRRAAALSGPPPLLPRVEDIALPTGLRARQYWPALDPALPLTVYFHGGR
FFSGDLESHDTICRLLALASASRVLAVDYRLAPEHPFPAAVEDARTALAWALLQDVPVAVAGDSAGANLAAVAALDHRHS
LRLRCQLLIYPMIDAVCASHSYTEFAEGYGPGAADMRRGWSEYLPAGSDPRDPLASPLYTPDLTGAAPALVITAEYDTLR
DEAESYARRLSAPVQRYPGAIHGFFLMQSPLALARQSLQDAAAFLRHHL

Sequences:

>Translated_289_residues
MPLDPEVAAHLELQRHLPPRSSLDIAATREMMRRAAALSGPPPLLPRVEDIALPTGLRARQYWPALDPALPLTVYFHGGR
FFSGDLESHDTICRLLALASASRVLAVDYRLAPEHPFPAAVEDARTALAWALLQDVPVAVAGDSAGANLAAVAALDHRHS
LRLRCQLLIYPMIDAVCASHSYTEFAEGYGPGAADMRRGWSEYLPAGSDPRDPLASPLYTPDLTGAAPALVITAEYDTLR
DEAESYARRLSAPVQRYPGAIHGFFLMQSPLALARQSLQDAAAFLRHHL
>Mature_288_residues
PLDPEVAAHLELQRHLPPRSSLDIAATREMMRRAAALSGPPPLLPRVEDIALPTGLRARQYWPALDPALPLTVYFHGGRF
FSGDLESHDTICRLLALASASRVLAVDYRLAPEHPFPAAVEDARTALAWALLQDVPVAVAGDSAGANLAAVAALDHRHSL
RLRCQLLIYPMIDAVCASHSYTEFAEGYGPGAADMRRGWSEYLPAGSDPRDPLASPLYTPDLTGAAPALVITAEYDTLRD
EAESYARRLSAPVQRYPGAIHGFFLMQSPLALARQSLQDAAAFLRHHL

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=163, Percent_Identity=31.2883435582822, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI206597554, Length=130, Percent_Identity=33.8461538461538, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI157041239, Length=116, Percent_Identity=38.7931034482759, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786682, Length=305, Percent_Identity=30.1639344262295, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17567059, Length=281, Percent_Identity=29.1814946619217, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI72001146, Length=171, Percent_Identity=31.5789473684211, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 31394; Mature: 31263

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDPEVAAHLELQRHLPPRSSLDIAATREMMRRAAALSGPPPLLPRVEDIALPTGLRAR
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHH
QYWPALDPALPLTVYFHGGRFFSGDLESHDTICRLLALASASRVLAVDYRLAPEHPFPAA
HCCCCCCCCCCEEEEEECCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHH
VEDARTALAWALLQDVPVAVAGDSAGANLAAVAALDHRHSLRLRCQLLIYPMIDAVCASH
HHHHHHHHHHHHHHHCCEEEECCCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
SYTEFAEGYGPGAADMRRGWSEYLPAGSDPRDPLASPLYTPDLTGAAPALVITAEYDTLR
CHHHHHHCCCCCHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHH
DEAESYARRLSAPVQRYPGAIHGFFLMQSPLALARQSLQDAAAFLRHHL
HHHHHHHHHHHCHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLDPEVAAHLELQRHLPPRSSLDIAATREMMRRAAALSGPPPLLPRVEDIALPTGLRAR
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHH
QYWPALDPALPLTVYFHGGRFFSGDLESHDTICRLLALASASRVLAVDYRLAPEHPFPAA
HCCCCCCCCCCEEEEEECCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHH
VEDARTALAWALLQDVPVAVAGDSAGANLAAVAALDHRHSLRLRCQLLIYPMIDAVCASH
HHHHHHHHHHHHHHHCCEEEECCCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
SYTEFAEGYGPGAADMRRGWSEYLPAGSDPRDPLASPLYTPDLTGAAPALVITAEYDTLR
CHHHHHHCCCCCHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHH
DEAESYARRLSAPVQRYPGAIHGFFLMQSPLALARQSLQDAAAFLRHHL
HHHHHHHHHHHCHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]