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The map label for this gene is fhlA [H]

Identifier: 116621171

GI number: 116621171

Start: 2606613

End: 2608733

Strand: Reverse

Name: fhlA [H]

Synonym: Acid_2052

Alternate gene names: 116621171

Gene position: 2608733-2606613 (Counterclockwise)

Preceding gene: 116621172

Following gene: 116621170

Centisome position: 26.18

GC content: 61.29

Gene sequence:

>2121_bases
ATGGGGACTATGTGGGCAGCTCCGGCCATCCGTGCCGTTTCACTTTCCGAGGGGCAGTCGTCCGAACGGTATGAGGCCTT
GATTCGGGTGTCGCAGGCGATCGGCGCACATCGGGATCCGAAAGAACTTTTTCGCGCGATGACCAGGGAATTGCACCGGG
TGATGCAGTTCGATGGTATCGTCATCGCCCAGTATGATGAGGCGTCCAACGAGATTCTCTGGAACGCCTGCGAGGTCTGC
AGTCAGCAGGGCGCGGTCCGGCCGCCCGATATTCCCGCCGATGAAACCATCACGAAGTGGGTCTATCAGCGCCAGGAGCC
GCTGGTGATTCCGGCCCTCGATCAGGAAACACGCTTTCCGCGGATGATCGAGTTTCTGCGAAGTAAGGGCTTTCAATCCA
TCTGCGCCCTTCCGCTGACTACGGTTCACCGCCGCATCGGCAGCGTCGCGGTCGCCAGCAAGCGGCCCGATGCCTATTGC
AAAGAGGAGGTGCGATTCCTCTCGCTGGTGGCGGACCAGGCGGCGCTCGCGATCGATGATGCCCTGAATTTTGAGACCTC
CCAGGTCGCCCAGGCAGCCCTGCAGCGGAAGAACGATCGTTTGCGGCTGCTCCTCGAAGTGAATAACAGCATTGCCTCGA
ATCTGGAGCTCCGCGAACTTCTGCGGGCGATCTCGGCCAGCATTCGCCCGGTAATGCAGTGCGATGGCGTGGGGGTCGCG
TTACCCGAAGGTGAGAATCAACAACTCCGCGTGTACGCCCTGGATTTCCCGAACGGCAACGGCCGCGTTCGCGAGGACAT
CTTAATACCTGCCAACGAAGATTCTCCGGGCGCGCGGGCCTTTCAAAACGGCGAACCGGCGAATATCCTCTCCGCCAGCA
TCGATCCCACAGATCCCCTGGCCACCGCGGAAGGCCTCAAGGCCGTCTGTCACATCCCCCTGATTAGCAGGAACCGCACC
GTCGGACTATTGAGCCTGGCTCGGCTGGAGCCCGTCGCCTTTTCCCCGCGGGATATCGAGTTCCTGAGCCTGGTAGGCAA
CCAGGTTGCGATCGCGGTGGAAAATGCGCTCGCCTATCACGAGATCTCCGCGTTGAAGGATAGATTGGCTCAGGAAAAGA
TCTATCTCGAAGATGAAATCCGCAGCGAGCTGAATTTCGAAGAGATCGTGGGCCGGAGCCAGGCATTGCGCACCGTGCTC
CAGGAGATCGAAACAGTGGCGCCCACCGATTCCACCGTGCTGATCTACGGCGAGACCGGGACCGGAAAAGAGCTGATCGC
GCGCGCCATCCACAACCTGAGTTCGCGCAGCCGGAATTCGTTCGTCAAACTGAACTGCGCCGCGATTCCCACGGGGCTGC
TCGAAAGCGAAATGTTCGGCCACGAGAAGGGAGCCTTTACGGGCGCGGTCGCCCAGCGTATCGGACGCTTCGAGCTGGCA
CATCATGGCACGGTGTTCCTGGATGAAATCGGCGAGATCCCCCTGGAGCTCCAGCCGAAACTCCTGCGTGTGCTTCAGGA
GCGGGAGTTCGAACGCTTGGGAAGCTCGCGCACCATGCGCACGGACGCGCGCTTGATCGCCGCCACCAATCGCGATCTCG
CAACCATGGTGGAGGATCGTAACTTCCGTGCCGATCTTTTTTACCGGTTGAACGTCTTTCCCGTACAGGTTCCGGCGCTG
CGCGATCGGCCCGAAGACATACCGCTTCTGGTCCGCCATTTTGTGCAGCAGTTCGCGCGGCGCATGGGCAAAGCGGTGGA
TACGATTCCCACCGAGACCATGAACGTCCTCGTACGGTATCATTGGCCGGGAAATATTCGCGAGCTCCAGAACCTGTTGG
AGCGAGCCGTAATCCTGTCGGCCGGACCGGTCCTGAAGGTGCCTCTCAACGATTTACAGCTACAGGCGCCTGCGCCCACC
ACCGCCAGGAAGACCGAGACCCTCGAGTCGGCAGAGCGCCGGCACATCCTGGAAGCGCTCGAGGCGGCCGACTGGGTGAT
CAGCGGACCAAAGGGCGCCGCGGAGGCATTAGGACTGAAGCGGAGTACGCTGCAGGCCCGCATGGAAAAACTGGGGATTC
GCCGCGCTCGTGCCGCCGCGCCTTCCCGTTCCGCGGGGTGA

Upstream 100 bases:

>100_bases
CCGGGTTCTACCGGCCCCCATGAATCAGGTGCGCCGTGTGGTTTTACACTTCGGCGTCAGGTTAGCGAATCGCGACCCGG
CCGCGAGGCATCTAGCTCAC

Downstream 100 bases:

>100_bases
ACTCATGAACCTTATGATCGGCTCTCTCTCCAGCAAAAGCGGACCGGTCTTCGGCGGGTTATACCAACAACTCCTCCGGC
GAGGCATTCAGTATTTCCTG

Product: NifA subfamily transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MGTMWAAPAIRAVSLSEGQSSERYEALIRVSQAIGAHRDPKELFRAMTRELHRVMQFDGIVIAQYDEASNEILWNACEVC
SQQGAVRPPDIPADETITKWVYQRQEPLVIPALDQETRFPRMIEFLRSKGFQSICALPLTTVHRRIGSVAVASKRPDAYC
KEEVRFLSLVADQAALAIDDALNFETSQVAQAALQRKNDRLRLLLEVNNSIASNLELRELLRAISASIRPVMQCDGVGVA
LPEGENQQLRVYALDFPNGNGRVREDILIPANEDSPGARAFQNGEPANILSASIDPTDPLATAEGLKAVCHIPLISRNRT
VGLLSLARLEPVAFSPRDIEFLSLVGNQVAIAVENALAYHEISALKDRLAQEKIYLEDEIRSELNFEEIVGRSQALRTVL
QEIETVAPTDSTVLIYGETGTGKELIARAIHNLSSRSRNSFVKLNCAAIPTGLLESEMFGHEKGAFTGAVAQRIGRFELA
HHGTVFLDEIGEIPLELQPKLLRVLQEREFERLGSSRTMRTDARLIAATNRDLATMVEDRNFRADLFYRLNVFPVQVPAL
RDRPEDIPLLVRHFVQQFARRMGKAVDTIPTETMNVLVRYHWPGNIRELQNLLERAVILSAGPVLKVPLNDLQLQAPAPT
TARKTETLESAERRHILEALEAADWVISGPKGAAEALGLKRSTLQARMEKLGIRRARAAAPSRSAG

Sequences:

>Translated_706_residues
MGTMWAAPAIRAVSLSEGQSSERYEALIRVSQAIGAHRDPKELFRAMTRELHRVMQFDGIVIAQYDEASNEILWNACEVC
SQQGAVRPPDIPADETITKWVYQRQEPLVIPALDQETRFPRMIEFLRSKGFQSICALPLTTVHRRIGSVAVASKRPDAYC
KEEVRFLSLVADQAALAIDDALNFETSQVAQAALQRKNDRLRLLLEVNNSIASNLELRELLRAISASIRPVMQCDGVGVA
LPEGENQQLRVYALDFPNGNGRVREDILIPANEDSPGARAFQNGEPANILSASIDPTDPLATAEGLKAVCHIPLISRNRT
VGLLSLARLEPVAFSPRDIEFLSLVGNQVAIAVENALAYHEISALKDRLAQEKIYLEDEIRSELNFEEIVGRSQALRTVL
QEIETVAPTDSTVLIYGETGTGKELIARAIHNLSSRSRNSFVKLNCAAIPTGLLESEMFGHEKGAFTGAVAQRIGRFELA
HHGTVFLDEIGEIPLELQPKLLRVLQEREFERLGSSRTMRTDARLIAATNRDLATMVEDRNFRADLFYRLNVFPVQVPAL
RDRPEDIPLLVRHFVQQFARRMGKAVDTIPTETMNVLVRYHWPGNIRELQNLLERAVILSAGPVLKVPLNDLQLQAPAPT
TARKTETLESAERRHILEALEAADWVISGPKGAAEALGLKRSTLQARMEKLGIRRARAAAPSRSAG
>Mature_705_residues
GTMWAAPAIRAVSLSEGQSSERYEALIRVSQAIGAHRDPKELFRAMTRELHRVMQFDGIVIAQYDEASNEILWNACEVCS
QQGAVRPPDIPADETITKWVYQRQEPLVIPALDQETRFPRMIEFLRSKGFQSICALPLTTVHRRIGSVAVASKRPDAYCK
EEVRFLSLVADQAALAIDDALNFETSQVAQAALQRKNDRLRLLLEVNNSIASNLELRELLRAISASIRPVMQCDGVGVAL
PEGENQQLRVYALDFPNGNGRVREDILIPANEDSPGARAFQNGEPANILSASIDPTDPLATAEGLKAVCHIPLISRNRTV
GLLSLARLEPVAFSPRDIEFLSLVGNQVAIAVENALAYHEISALKDRLAQEKIYLEDEIRSELNFEEIVGRSQALRTVLQ
EIETVAPTDSTVLIYGETGTGKELIARAIHNLSSRSRNSFVKLNCAAIPTGLLESEMFGHEKGAFTGAVAQRIGRFELAH
HGTVFLDEIGEIPLELQPKLLRVLQEREFERLGSSRTMRTDARLIAATNRDLATMVEDRNFRADLFYRLNVFPVQVPALR
DRPEDIPLLVRHFVQQFARRMGKAVDTIPTETMNVLVRYHWPGNIRELQNLLERAVILSAGPVLKVPLNDLQLQAPAPTT
ARKTETLESAERRHILEALEAADWVISGPKGAAEALGLKRSTLQARMEKLGIRRARAAAPSRSAG

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=660, Percent_Identity=40.9090909090909, Blast_Score=471, Evalue=1e-133,
Organism=Escherichia coli, GI87082117, Length=559, Percent_Identity=42.5760286225402, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1788550, Length=335, Percent_Identity=45.0746268656716, Blast_Score=285, Evalue=1e-77,
Organism=Escherichia coli, GI1790437, Length=307, Percent_Identity=50.814332247557, Blast_Score=281, Evalue=9e-77,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=51.931330472103, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=41.2307692307692, Blast_Score=241, Evalue=2e-64,
Organism=Escherichia coli, GI1788905, Length=239, Percent_Identity=49.7907949790795, Blast_Score=240, Evalue=3e-64,
Organism=Escherichia coli, GI1790299, Length=262, Percent_Identity=46.1832061068702, Blast_Score=232, Evalue=6e-62,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=39.6875, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1786524, Length=327, Percent_Identity=39.1437308868502, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1787583, Length=266, Percent_Identity=39.0977443609023, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1789828, Length=273, Percent_Identity=34.4322344322344, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI87081858, Length=286, Percent_Identity=33.9160839160839, Blast_Score=134, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78362; Mature: 78231

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTMWAAPAIRAVSLSEGQSSERYEALIRVSQAIGAHRDPKELFRAMTRELHRVMQFDGI
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCE
VIAQYDEASNEILWNACEVCSQQGAVRPPDIPADETITKWVYQRQEPLVIPALDQETRFP
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCH
RMIEFLRSKGFQSICALPLTTVHRRIGSVAVASKRPDAYCKEEVRFLSLVADQAALAIDD
HHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALNFETSQVAQAALQRKNDRLRLLLEVNNSIASNLELRELLRAISASIRPVMQCDGVGVA
HCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHCCHHHEECCCEEE
LPEGENQQLRVYALDFPNGNGRVREDILIPANEDSPGARAFQNGEPANILSASIDPTDPL
CCCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCC
ATAEGLKAVCHIPLISRNRTVGLLSLARLEPVAFSPRDIEFLSLVGNQVAIAVENALAYH
HHHHHHHHHEECEEECCCCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHH
EISALKDRLAQEKIYLEDEIRSELNFEEIVGRSQALRTVLQEIETVAPTDSTVLIYGETG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEECCC
TGKELIARAIHNLSSRSRNSFVKLNCAAIPTGLLESEMFGHEKGAFTGAVAQRIGRFELA
CCHHHHHHHHHHHHHCCCCCEEEEEEHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
HHGTVFLDEIGEIPLELQPKLLRVLQEREFERLGSSRTMRTDARLIAATNRDLATMVEDR
CCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCEEEEECCCHHHHHHHCC
NFRADLFYRLNVFPVQVPALRDRPEDIPLLVRHFVQQFARRMGKAVDTIPTETMNVLVRY
CCCEEEEEEEEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
HWPGNIRELQNLLERAVILSAGPVLKVPLNDLQLQAPAPTTARKTETLESAERRHILEAL
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EAADWVISGPKGAAEALGLKRSTLQARMEKLGIRRARAAAPSRSAG
HHHCCEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GTMWAAPAIRAVSLSEGQSSERYEALIRVSQAIGAHRDPKELFRAMTRELHRVMQFDGI
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCE
VIAQYDEASNEILWNACEVCSQQGAVRPPDIPADETITKWVYQRQEPLVIPALDQETRFP
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCH
RMIEFLRSKGFQSICALPLTTVHRRIGSVAVASKRPDAYCKEEVRFLSLVADQAALAIDD
HHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALNFETSQVAQAALQRKNDRLRLLLEVNNSIASNLELRELLRAISASIRPVMQCDGVGVA
HCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHCCHHHEECCCEEE
LPEGENQQLRVYALDFPNGNGRVREDILIPANEDSPGARAFQNGEPANILSASIDPTDPL
CCCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCC
ATAEGLKAVCHIPLISRNRTVGLLSLARLEPVAFSPRDIEFLSLVGNQVAIAVENALAYH
HHHHHHHHHEECEEECCCCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHH
EISALKDRLAQEKIYLEDEIRSELNFEEIVGRSQALRTVLQEIETVAPTDSTVLIYGETG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEECCC
TGKELIARAIHNLSSRSRNSFVKLNCAAIPTGLLESEMFGHEKGAFTGAVAQRIGRFELA
CCHHHHHHHHHHHHHCCCCCEEEEEEHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
HHGTVFLDEIGEIPLELQPKLLRVLQEREFERLGSSRTMRTDARLIAATNRDLATMVEDR
CCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCEEEEECCCHHHHHHHCC
NFRADLFYRLNVFPVQVPALRDRPEDIPLLVRHFVQQFARRMGKAVDTIPTETMNVLVRY
CCCEEEEEEEEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
HWPGNIRELQNLLERAVILSAGPVLKVPLNDLQLQAPAPTTARKTETLESAERRHILEAL
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EAADWVISGPKGAAEALGLKRSTLQARMEKLGIRRARAAAPSRSAG
HHHCCEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]