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The map label for this gene is dapE [H]

Identifier: 116621069

GI number: 116621069

Start: 2436850

End: 2438238

Strand: Reverse

Name: dapE [H]

Synonym: Acid_1950

Alternate gene names: 116621069

Gene position: 2438238-2436850 (Counterclockwise)

Preceding gene: 116621070

Following gene: 116621063

Centisome position: 24.47

GC content: 62.06

Gene sequence:

>1389_bases
GTGAGATCCGCGGCCGCGCTGCTGGCGGCGCTGTGTCTGCCAGGTGTGACACTGGCGCAGCCCGCGGTAGCGGATCTGCT
GGCACGCCTCATCCGCGTGGACACCAGCAATCCTCCGGGGAACGAGGCCAGGCTCGCCGAGTTGCTGGCCGCGGAGTTCA
AGCCGCTCGGTTTCCAAATCGAGATAGTGCCCGCCCCGGAGGGCAAGGCCCACTTCATCGCGCGACTGAAAGGCGATGGC
TCAAAGCGCCCCGTGCTCCTGGCTGCGCACGCCGATGTGGTCGGGGTAGAGAGGGAGAAGTGGTCGGTGGATCCCTTCGC
GGGCCAGATCAAAGATGGGTACGTCTTCGGTCGCGGCGCCATCGATTTCAAAGGCGGGTTGGCGGTCTTCGCTCGGGCAG
TGATGATGCTGGCGATCAACAAGGTCCCCCTTCATCGTGACGTCATTTTCCTTTCCGAGTCCGATGAAGAGGGCGGCCGC
TACAATACGTCCTGGCTGGCGGCGGACCACTGGGACAAAATCGATTGCGATTTCGCCTTAAACGAAGGGGGCTGGATTAT
GGAGAACCCGGATGGCACGGTACGCTATGTCAGCATCTCCACCGCCGACAAGAGCGGCGTCACACTCCTGATCACCGCCA
AAGGCACCTCGACTCATTCCTCCATGCCGCGGCCCGACAACGCGATCTTTGCCCTCGCCAGGGCGATGGCGAAGATCGCG
GACTACGATACGCATCCCAAGCTGATCGAAAGCACTCGCGAATTCTTCCTCACCCTGGCGAAAACCAGCGCTCCACCGGA
ATCGACCTATTTCGAAAACCTGGTAAAAAGCGATGACCCGGTGCTGGTTCAGCGGGCTGACAAAGAAATCAGCAAGGACC
CGCTGTTGCACGCGATCATGCGTAACACCATCGCCCCTGTCTTCCTCAAGGCCGGTTTTCGCGGTAACGTGATTCCCGGC
TCGGCGGAAGCGACCATCAACTTCCGTACGATCCCCGGCACGAATCCGCAGGAGTTGATCTCGGAGATCAAGGCGGTGAT
CAATGATCCGGAAATCAGCGTCACGTTGGCCGCGACCGGCAATCGCCCCGCCACTGCGGGTCCCCGCTCCATGGAGTCGA
GCAAGGACACTGACCTTTACCGCGCCCTGGTGCGAGCGGCGAATAAGGTCTGGCCCGGCGCGCCGGTGACCCCGTATCTT
TTCCAGGCCGGCACCGACGCCATGGCGTGGCGCAGTCGCGGCGTTCCGGTGTACGGCATCTATCCCTACCCGATCTCCGC
CGAGGACCTCACCCGCATGCACGGCAATGACGAGCGTGTGGCGATCGCTTCACTCGAGCAGGGCGTGGACCTGATCTATC
GCACTTTAGTGGAGGTCGCGGCCAGATAA

Upstream 100 bases:

>100_bases
TGGAGAGCAGTTGGAAAAAGATTTCGGAAGACGAGGTGGCTGGCCTAAACCGCAGTCTCTCCTCGGCGGGCGTCTCTTAT
CTGGCCGCCGGAGGCTGGTA

Downstream 100 bases:

>100_bases
CCCGGCCTGCGGACGGGCTGGCTGTCCATACCTGGAAGAAGATGACTGTTTTCTTTAAGGCAGTGAAACGGCGATCGTAG
GAAGCACCCGTGTTCGCAGG

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MRSAAALLAALCLPGVTLAQPAVADLLARLIRVDTSNPPGNEARLAELLAAEFKPLGFQIEIVPAPEGKAHFIARLKGDG
SKRPVLLAAHADVVGVEREKWSVDPFAGQIKDGYVFGRGAIDFKGGLAVFARAVMMLAINKVPLHRDVIFLSESDEEGGR
YNTSWLAADHWDKIDCDFALNEGGWIMENPDGTVRYVSISTADKSGVTLLITAKGTSTHSSMPRPDNAIFALARAMAKIA
DYDTHPKLIESTREFFLTLAKTSAPPESTYFENLVKSDDPVLVQRADKEISKDPLLHAIMRNTIAPVFLKAGFRGNVIPG
SAEATINFRTIPGTNPQELISEIKAVINDPEISVTLAATGNRPATAGPRSMESSKDTDLYRALVRAANKVWPGAPVTPYL
FQAGTDAMAWRSRGVPVYGIYPYPISAEDLTRMHGNDERVAIASLEQGVDLIYRTLVEVAAR

Sequences:

>Translated_462_residues
MRSAAALLAALCLPGVTLAQPAVADLLARLIRVDTSNPPGNEARLAELLAAEFKPLGFQIEIVPAPEGKAHFIARLKGDG
SKRPVLLAAHADVVGVEREKWSVDPFAGQIKDGYVFGRGAIDFKGGLAVFARAVMMLAINKVPLHRDVIFLSESDEEGGR
YNTSWLAADHWDKIDCDFALNEGGWIMENPDGTVRYVSISTADKSGVTLLITAKGTSTHSSMPRPDNAIFALARAMAKIA
DYDTHPKLIESTREFFLTLAKTSAPPESTYFENLVKSDDPVLVQRADKEISKDPLLHAIMRNTIAPVFLKAGFRGNVIPG
SAEATINFRTIPGTNPQELISEIKAVINDPEISVTLAATGNRPATAGPRSMESSKDTDLYRALVRAANKVWPGAPVTPYL
FQAGTDAMAWRSRGVPVYGIYPYPISAEDLTRMHGNDERVAIASLEQGVDLIYRTLVEVAAR
>Mature_462_residues
MRSAAALLAALCLPGVTLAQPAVADLLARLIRVDTSNPPGNEARLAELLAAEFKPLGFQIEIVPAPEGKAHFIARLKGDG
SKRPVLLAAHADVVGVEREKWSVDPFAGQIKDGYVFGRGAIDFKGGLAVFARAVMMLAINKVPLHRDVIFLSESDEEGGR
YNTSWLAADHWDKIDCDFALNEGGWIMENPDGTVRYVSISTADKSGVTLLITAKGTSTHSSMPRPDNAIFALARAMAKIA
DYDTHPKLIESTREFFLTLAKTSAPPESTYFENLVKSDDPVLVQRADKEISKDPLLHAIMRNTIAPVFLKAGFRGNVIPG
SAEATINFRTIPGTNPQELISEIKAVINDPEISVTLAATGNRPATAGPRSMESSKDTDLYRALVRAANKVWPGAPVTPYL
FQAGTDAMAWRSRGVPVYGIYPYPISAEDLTRMHGNDERVAIASLEQGVDLIYRTLVEVAAR

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI269846968, Length=403, Percent_Identity=28.287841191067, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1790395, Length=211, Percent_Identity=30.3317535545024, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322289, Length=266, Percent_Identity=28.1954887218045, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24649210, Length=442, Percent_Identity=23.5294117647059, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24649208, Length=439, Percent_Identity=22.0956719817768, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24649212, Length=222, Percent_Identity=25.6756756756757, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50031; Mature: 50031

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSAAALLAALCLPGVTLAQPAVADLLARLIRVDTSNPPGNEARLAELLAAEFKPLGFQI
CCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEE
EIVPAPEGKAHFIARLKGDGSKRPVLLAAHADVVGVEREKWSVDPFAGQIKDGYVFGRGA
EEEECCCCCEEEEEEEECCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCEEEECCC
IDFKGGLAVFARAVMMLAINKVPLHRDVIFLSESDEEGGRYNTSWLAADHWDKIDCDFAL
EECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEEE
NEGGWIMENPDGTVRYVSISTADKSGVTLLITAKGTSTHSSMPRPDNAIFALARAMAKIA
CCCCEEEECCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
DYDTHPKLIESTREFFLTLAKTSAPPESTYFENLVKSDDPVLVQRADKEISKDPLLHAIM
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHHHH
RNTIAPVFLKAGFRGNVIPGSAEATINFRTIPGTNPQELISEIKAVINDPEISVTLAATG
HHHHHHHHEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECC
NRPATAGPRSMESSKDTDLYRALVRAANKVWPGAPVTPYLFQAGTDAMAWRSRGVPVYGI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHCCCCCEEEE
YPYPISAEDLTRMHGNDERVAIASLEQGVDLIYRTLVEVAAR
ECCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSAAALLAALCLPGVTLAQPAVADLLARLIRVDTSNPPGNEARLAELLAAEFKPLGFQI
CCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEE
EIVPAPEGKAHFIARLKGDGSKRPVLLAAHADVVGVEREKWSVDPFAGQIKDGYVFGRGA
EEEECCCCCEEEEEEEECCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCEEEECCC
IDFKGGLAVFARAVMMLAINKVPLHRDVIFLSESDEEGGRYNTSWLAADHWDKIDCDFAL
EECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEEE
NEGGWIMENPDGTVRYVSISTADKSGVTLLITAKGTSTHSSMPRPDNAIFALARAMAKIA
CCCCEEEECCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
DYDTHPKLIESTREFFLTLAKTSAPPESTYFENLVKSDDPVLVQRADKEISKDPLLHAIM
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHHHH
RNTIAPVFLKAGFRGNVIPGSAEATINFRTIPGTNPQELISEIKAVINDPEISVTLAATG
HHHHHHHHEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECC
NRPATAGPRSMESSKDTDLYRALVRAANKVWPGAPVTPYLFQAGTDAMAWRSRGVPVYGI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHCCCCCEEEE
YPYPISAEDLTRMHGNDERVAIASLEQGVDLIYRTLVEVAAR
ECCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA