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The map label for this gene is steT [H]

Identifier: 116621046

GI number: 116621046

Start: 2392488

End: 2393858

Strand: Reverse

Name: steT [H]

Synonym: Acid_1927

Alternate gene names: 116621046

Gene position: 2393858-2392488 (Counterclockwise)

Preceding gene: 116621048

Following gene: 116621045

Centisome position: 24.02

GC content: 59.52

Gene sequence:

>1371_bases
TTGACCCAGACTGAGTCTCCGCATCTCGTACGCGGCATCGGCCTTCTCGGAGCCGTCTCAGCCAATATGCTGGAGATGAT
CGGAGTGGGTCCGTTCATCACCATTCCGATTCTGCTTGCGAAAATGAACGGACCGCAGGCGATTCTCGGTTGGTTGTTGG
GCGCCCTGGTCGCGCTTTGCGACGGCATGGTCTGGGCCGAACTGGGAGCCGCCATGCCGGGCACCGGAGGACCGTATCAT
TACCTCTCCGAGGCATATGGGCCTCAGCGGATGGGCCGCCTCATGAGCTTTCTGTTCATCTGGCAAACAATGGCGCTGGC
TCCGCTTTCCATCGGTTCCGGCGCCGTCGGCTTTGCCCAATACGCCAGGTTTCTATTCAAAGACATCACTCCGCTCCAGG
AAAAGCTGATTGCCGTGAGTGTCTGCGCGCTGATCACGGTGCTGCTCTACCGGGACATTCGTTCGGTCGGCCGGCTTTCG
ATCATCATGTGGATTGTCGTGGTCGGCACGGTACTCTGGATTACGGCGGCGGGAGTAATGCACTTTGATCCGCGGCGTGT
CCTGGATTTTCCGTCGGGCGCGTTCACACCCTCGCGAAGCTTTTTCTTCGGCCTCGGCGGCGCGACTTTGATTGCCATGT
ACGATTACGGCGGATACAACAACGTCTGCTTTTTCGCGGGTGAAGTGCGCCGTCCGGAGCGGGTCATCCCACGATCCATC
CTGTTGTCGATTGCCGCGGTAGCCACGCTCTACCTGACGATGAACATCACCATCATCGGCGTGGTGCCCTGGCGGGAAGC
GATCCGCTCCACCGCCATTGCCTCCGATTTTATCCAGAGAATTTACGGCGCGAAGGCCGCCGCCGTGGTCACCGTTTTGA
TCCTGTGGACCACATTCGCCTCGGTCTTCGCCGTCCTCCTGGGCTATTCGCGCGTACCGTATGCAGCCGCGGTGGACGGC
CGGTTCTTCCGGCCCTTCGCCCGCCTGCACTCATCCAAGAACTTCCCGTATTTCTCAGTGCTCTTCATCGGAGTTCTATC
GGCGGCAGCATCGTTGTTGACTCTGGATGTGTTGATCAACGGTCTGATTGTGATTCAGGTGCTGATCCAATTCATGGCGC
AGATATTCGCGGTCACACTCATCCGGCGAGATCGGCCGGACATTGTGCGGCCTTTCAAAATGCCTCTGTACCCGCTCCCC
AGCATCATCGCGTTTCTAGGTTGGCTGTACATTCTGATCGCCAGCGGACTGGCCTACATCGTCGCAGGCTTGGTGCTCAT
GATGTTGGGCGTGGCCGCCTACGTTTGGCACGCCCACGGGGCTGGCGAGTGGCCTTACGCCAGCCGGTGGAAGGCGGGTG
CGGATGCCTGA

Upstream 100 bases:

>100_bases
CAGCCTGCTCCAACGGCGATTGGGCCAGTGCTCCGATCGCCGTCGCCAGCGCGAAAATGAGAGTCGAGCGGATCGTACCA
ACTATGATAACTTGGGCAAA

Downstream 100 bases:

>100_bases
CCCGAGGGCTTTCGACGCGATCGTCGCCGGTGAACTCTATACCGACCTGATCCTAAGCGGATTCGATTTCTGGCCGCGGC
CCGGCCAGGAGGCCTTCGCC

Product: amino acid permease-associated protein

Products: Proton [Cytoplasm]; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTQTESPHLVRGIGLLGAVSANMLEMIGVGPFITIPILLAKMNGPQAILGWLLGALVALCDGMVWAELGAAMPGTGGPYH
YLSEAYGPQRMGRLMSFLFIWQTMALAPLSIGSGAVGFAQYARFLFKDITPLQEKLIAVSVCALITVLLYRDIRSVGRLS
IIMWIVVVGTVLWITAAGVMHFDPRRVLDFPSGAFTPSRSFFFGLGGATLIAMYDYGGYNNVCFFAGEVRRPERVIPRSI
LLSIAAVATLYLTMNITIIGVVPWREAIRSTAIASDFIQRIYGAKAAAVVTVLILWTTFASVFAVLLGYSRVPYAAAVDG
RFFRPFARLHSSKNFPYFSVLFIGVLSAAASLLTLDVLINGLIVIQVLIQFMAQIFAVTLIRRDRPDIVRPFKMPLYPLP
SIIAFLGWLYILIASGLAYIVAGLVLMMLGVAAYVWHAHGAGEWPYASRWKAGADA

Sequences:

>Translated_456_residues
MTQTESPHLVRGIGLLGAVSANMLEMIGVGPFITIPILLAKMNGPQAILGWLLGALVALCDGMVWAELGAAMPGTGGPYH
YLSEAYGPQRMGRLMSFLFIWQTMALAPLSIGSGAVGFAQYARFLFKDITPLQEKLIAVSVCALITVLLYRDIRSVGRLS
IIMWIVVVGTVLWITAAGVMHFDPRRVLDFPSGAFTPSRSFFFGLGGATLIAMYDYGGYNNVCFFAGEVRRPERVIPRSI
LLSIAAVATLYLTMNITIIGVVPWREAIRSTAIASDFIQRIYGAKAAAVVTVLILWTTFASVFAVLLGYSRVPYAAAVDG
RFFRPFARLHSSKNFPYFSVLFIGVLSAAASLLTLDVLINGLIVIQVLIQFMAQIFAVTLIRRDRPDIVRPFKMPLYPLP
SIIAFLGWLYILIASGLAYIVAGLVLMMLGVAAYVWHAHGAGEWPYASRWKAGADA
>Mature_455_residues
TQTESPHLVRGIGLLGAVSANMLEMIGVGPFITIPILLAKMNGPQAILGWLLGALVALCDGMVWAELGAAMPGTGGPYHY
LSEAYGPQRMGRLMSFLFIWQTMALAPLSIGSGAVGFAQYARFLFKDITPLQEKLIAVSVCALITVLLYRDIRSVGRLSI
IMWIVVVGTVLWITAAGVMHFDPRRVLDFPSGAFTPSRSFFFGLGGATLIAMYDYGGYNNVCFFAGEVRRPERVIPRSIL
LSIAAVATLYLTMNITIIGVVPWREAIRSTAIASDFIQRIYGAKAAAVVTVLILWTTFASVFAVLLGYSRVPYAAAVDGR
FFRPFARLHSSKNFPYFSVLFIGVLSAAASLLTLDVLINGLIVIQVLIQFMAQIFAVTLIRRDRPDIVRPFKMPLYPLPS
IIAFLGWLYILIASGLAYIVAGLVLMMLGVAAYVWHAHGAGEWPYASRWKAGADA

Specific function: Exhibits an obligate exchange activity for serine, threonine and aromatic amino acids [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family [H]

Homologues:

Organism=Homo sapiens, GI7657683, Length=391, Percent_Identity=26.3427109974425, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI33286428, Length=446, Percent_Identity=25.7847533632287, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI187423910, Length=416, Percent_Identity=23.7980769230769, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI7657591, Length=416, Percent_Identity=23.7980769230769, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI71999149, Length=421, Percent_Identity=23.9904988123515, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI186910308, Length=412, Percent_Identity=24.0291262135922, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI186910306, Length=412, Percent_Identity=24.0291262135922, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI186910304, Length=412, Percent_Identity=24.0291262135922, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI115648063, Length=354, Percent_Identity=22.8813559322034, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI115648022, Length=354, Percent_Identity=22.8813559322034, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI9790235, Length=305, Percent_Identity=25.5737704918033, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI71979932, Length=402, Percent_Identity=23.8805970149254, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI33286430, Length=250, Percent_Identity=29.2, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786908, Length=447, Percent_Identity=23.7136465324385, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082250, Length=450, Percent_Identity=23.5555555555556, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI2367353, Length=455, Percent_Identity=23.956043956044, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786694, Length=281, Percent_Identity=25.9786476868327, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540018, Length=408, Percent_Identity=25.7352941176471, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32566699, Length=374, Percent_Identity=26.4705882352941, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71993915, Length=446, Percent_Identity=24.4394618834081, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71982612, Length=411, Percent_Identity=25.0608272506083, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71982608, Length=409, Percent_Identity=25.1833740831296, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI193208714, Length=373, Percent_Identity=23.8605898123324, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32566695, Length=373, Percent_Identity=23.8605898123324, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71995382, Length=368, Percent_Identity=23.9130434782609, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71995388, Length=368, Percent_Identity=23.9130434782609, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17568033, Length=446, Percent_Identity=25.1121076233184, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321492, Length=343, Percent_Identity=24.198250728863, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221331183, Length=465, Percent_Identity=24.7311827956989, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17647653, Length=465, Percent_Identity=24.7311827956989, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24664379, Length=465, Percent_Identity=24.7311827956989, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24641993, Length=401, Percent_Identity=26.1845386533666, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24641995, Length=407, Percent_Identity=25.5528255528256, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI45550968, Length=408, Percent_Identity=26.9607843137255, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19921172, Length=408, Percent_Identity=26.9607843137255, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21357367, Length=415, Percent_Identity=25.0602409638554, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24651635, Length=412, Percent_Identity=23.3009708737864, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24651633, Length=412, Percent_Identity=23.3009708737864, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 49687; Mature: 49556

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTESPHLVRGIGLLGAVSANMLEMIGVGPFITIPILLAKMNGPQAILGWLLGALVALC
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
DGMVWAELGAAMPGTGGPYHYLSEAYGPQRMGRLMSFLFIWQTMALAPLSIGSGAVGFAQ
CCHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
YARFLFKDITPLQEKLIAVSVCALITVLLYRDIRSVGRLSIIMWIVVVGTVLWITAAGVM
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFDPRRVLDFPSGAFTPSRSFFFGLGGATLIAMYDYGGYNNVCFFAGEVRRPERVIPRSI
CCCCHHHHCCCCCCCCCCCCCEEECCCHHEEEEEECCCCCCEEEEECCCCCCHHHHHHHH
LLSIAAVATLYLTMNITIIGVVPWREAIRSTAIASDFIQRIYGAKAAAVVTVLILWTTFA
HHHHHHHHHHHHHEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SVFAVLLGYSRVPYAAAVDGRFFRPFARLHSSKNFPYFSVLFIGVLSAAASLLTLDVLIN
HHHHHHHCCCCCCCHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLIVIQVLIQFMAQIFAVTLIRRDRPDIVRPFKMPLYPLPSIIAFLGWLYILIASGLAYI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAGLVLMMLGVAAYVWHAHGAGEWPYASRWKAGADA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQTESPHLVRGIGLLGAVSANMLEMIGVGPFITIPILLAKMNGPQAILGWLLGALVALC
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
DGMVWAELGAAMPGTGGPYHYLSEAYGPQRMGRLMSFLFIWQTMALAPLSIGSGAVGFAQ
CCHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
YARFLFKDITPLQEKLIAVSVCALITVLLYRDIRSVGRLSIIMWIVVVGTVLWITAAGVM
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFDPRRVLDFPSGAFTPSRSFFFGLGGATLIAMYDYGGYNNVCFFAGEVRRPERVIPRSI
CCCCHHHHCCCCCCCCCCCCCEEECCCHHEEEEEECCCCCCEEEEECCCCCCHHHHHHHH
LLSIAAVATLYLTMNITIIGVVPWREAIRSTAIASDFIQRIYGAKAAAVVTVLILWTTFA
HHHHHHHHHHHHHEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SVFAVLLGYSRVPYAAAVDGRFFRPFARLHSSKNFPYFSVLFIGVLSAAASLLTLDVLIN
HHHHHHHCCCCCCCHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLIVIQVLIQFMAQIFAVTLIRRDRPDIVRPFKMPLYPLPSIIAFLGWLYILIASGLAYI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAGLVLMMLGVAAYVWHAHGAGEWPYASRWKAGADA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; putrescine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + putrescine [Periplasm] = Proton [Cytoplasm] + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]