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The map label for this gene is glnA [H]

Identifier: 116621020

GI number: 116621020

Start: 2359407

End: 2360837

Strand: Reverse

Name: glnA [H]

Synonym: Acid_1901

Alternate gene names: 116621020

Gene position: 2360837-2359407 (Counterclockwise)

Preceding gene: 116621026

Following gene: 116621018

Centisome position: 23.69

GC content: 60.45

Gene sequence:

>1431_bases
ATGCCCAATCAACTCCAAGACTTTTTGACCCTCTCCTACGCGGAATTGGAAGAGTTGAATCTGCAGGCCAAAGCCGAGCG
CCTCGGCCGCGCGCCCACGCACCAGATTCAGGAGCAGCGCCTCAAGTACCTCACCGACGAGAAGCGCATCAAGGCCGTCA
CGGTCCTGTTCAGTGACCTGGAAGGCCGCCTCCACATGCTCGATTACGACAAGAAGTTCCTCGTGAAGAGCTGGGACAAC
CTGACCTTCGACGGCTCCTCCATCCGCGGCTTCACCGCGCAGCGCGAGAGCGACCTGCGTCTTTCGATGGATTGGAGTTC
CTTCTACTGGGCGCCTTCCGACGTGTTCGGCGCCGGCAAAGTGCTGGTCTTCGGCGAGGTGATCGATAAGGACGGCACGC
CCTACAGCGCCGACATCCGCGGCGTGCTCAAGGCGTACGCGCAGAGCCTCTATGAGAAGCAGGGCTACACGCTCAACGCC
GCCAACGAAATCGAGGGCTTCCTGTTCAACGGCTCCGATGCCGAGCGCCGCTACCACGAAACCGCGAAGTTCGATTACGT
CAATACCGGCGGCTACTACCACTCGCTGCCCGGGGACCCGCTCCGCACCTTCATCGACAGCTGCGCCGAAGTGCAGCGCG
CGATGGGCTTCCAGAATGAAAAGGATCACCCGGAAGTCGCGCCCTCCCAGTTCGAGATCAACTATAGCTACGCCGAGGTC
GTAGCCGCGGCCGACCAGATTCAGCTTTACAAGCTGATCTGCCGCCAGGTCGCCACCCGCATGGGCCTCACCGCCAGCTT
CCTGCCCAAGCCGGTGGTGGGCGTGAATGGCAGCGGCATGCACACCAACGTATCCATCACCAAGGGCGGCAAGAACATTT
TCTGGGATCCCAAAGGCGAAGAGAAAATGAGCAAATTCGCCTGGCAGTTCGTGGACCGCATCCTCACCCACGGCAACGAT
CTCTGCCTGCTGCTCAACGCCAGCGTCAACGCCTACCGCCGTCTCGACCCGCACTTCGAAGCGCCCAACCAGATTAAGGC
CTCGGCCGTGGATCGCGGCTCCATGATCCGCATTCCGATCGGCAACGAGCGCAGCTCACGCGTCGAAGTCCGCTCCGTCG
GCCCGGACGCGAATCCCTACATGGTGATGCTTGGCGTTTTCCGCACCGGCCTGGAAGGCGAAATCTCGCGCATTAAGAAT
CTCCGCGGCGCCGAACGCTACCTGCCCGACAACATCTACGACGCCATGGCCAATTTCAAGAACGCGGAATGGACCACCAA
ATTACTTGGCGAAGACGTTAAGGGCCGGTATGCCGACTTGAAGCAGGCATCCGCGGATCGCTGTCCCCGGCTGCTCGGCA
CTTTTGTCAAGACGCCCGAAGTTCAGTACCACCACGAAGTCTACAACCAGTTCCTCTGGAACATGTTCTAA

Upstream 100 bases:

>100_bases
GCGCCGGCTTGTGACTGATTAATTTCTTGACGGCGGCCCATAAAGACAATCTATCGCTGGCTCCTCGAAAACGATCGACA
ATTAAGTTTCACCCCTGATT

Downstream 100 bases:

>100_bases
CCCTGCGAATCCCTGGCGTGTGCCAGTGGGTACAGTGGCGAGCGGCAAACTGAACTTCCGAGAGAAGGAATTGCCCGCAT
CGAGGATGGACCGGAAGAAT

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MPNQLQDFLTLSYAELEELNLQAKAERLGRAPTHQIQEQRLKYLTDEKRIKAVTVLFSDLEGRLHMLDYDKKFLVKSWDN
LTFDGSSIRGFTAQRESDLRLSMDWSSFYWAPSDVFGAGKVLVFGEVIDKDGTPYSADIRGVLKAYAQSLYEKQGYTLNA
ANEIEGFLFNGSDAERRYHETAKFDYVNTGGYYHSLPGDPLRTFIDSCAEVQRAMGFQNEKDHPEVAPSQFEINYSYAEV
VAAADQIQLYKLICRQVATRMGLTASFLPKPVVGVNGSGMHTNVSITKGGKNIFWDPKGEEKMSKFAWQFVDRILTHGND
LCLLLNASVNAYRRLDPHFEAPNQIKASAVDRGSMIRIPIGNERSSRVEVRSVGPDANPYMVMLGVFRTGLEGEISRIKN
LRGAERYLPDNIYDAMANFKNAEWTTKLLGEDVKGRYADLKQASADRCPRLLGTFVKTPEVQYHHEVYNQFLWNMF

Sequences:

>Translated_476_residues
MPNQLQDFLTLSYAELEELNLQAKAERLGRAPTHQIQEQRLKYLTDEKRIKAVTVLFSDLEGRLHMLDYDKKFLVKSWDN
LTFDGSSIRGFTAQRESDLRLSMDWSSFYWAPSDVFGAGKVLVFGEVIDKDGTPYSADIRGVLKAYAQSLYEKQGYTLNA
ANEIEGFLFNGSDAERRYHETAKFDYVNTGGYYHSLPGDPLRTFIDSCAEVQRAMGFQNEKDHPEVAPSQFEINYSYAEV
VAAADQIQLYKLICRQVATRMGLTASFLPKPVVGVNGSGMHTNVSITKGGKNIFWDPKGEEKMSKFAWQFVDRILTHGND
LCLLLNASVNAYRRLDPHFEAPNQIKASAVDRGSMIRIPIGNERSSRVEVRSVGPDANPYMVMLGVFRTGLEGEISRIKN
LRGAERYLPDNIYDAMANFKNAEWTTKLLGEDVKGRYADLKQASADRCPRLLGTFVKTPEVQYHHEVYNQFLWNMF
>Mature_475_residues
PNQLQDFLTLSYAELEELNLQAKAERLGRAPTHQIQEQRLKYLTDEKRIKAVTVLFSDLEGRLHMLDYDKKFLVKSWDNL
TFDGSSIRGFTAQRESDLRLSMDWSSFYWAPSDVFGAGKVLVFGEVIDKDGTPYSADIRGVLKAYAQSLYEKQGYTLNAA
NEIEGFLFNGSDAERRYHETAKFDYVNTGGYYHSLPGDPLRTFIDSCAEVQRAMGFQNEKDHPEVAPSQFEINYSYAEVV
AAADQIQLYKLICRQVATRMGLTASFLPKPVVGVNGSGMHTNVSITKGGKNIFWDPKGEEKMSKFAWQFVDRILTHGNDL
CLLLNASVNAYRRLDPHFEAPNQIKASAVDRGSMIRIPIGNERSSRVEVRSVGPDANPYMVMLGVFRTGLEGEISRIKNL
RGAERYLPDNIYDAMANFKNAEWTTKLLGEDVKGRYADLKQASADRCPRLLGTFVKTPEVQYHHEVYNQFLWNMF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=400, Percent_Identity=31.5, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI87081870, Length=441, Percent_Identity=27.891156462585, Blast_Score=115, Evalue=9e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 54056; Mature: 53925

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNQLQDFLTLSYAELEELNLQAKAERLGRAPTHQIQEQRLKYLTDEKRIKAVTVLFSDL
CCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHC
EGRLHMLDYDKKFLVKSWDNLTFDGSSIRGFTAQRESDLRLSMDWSSFYWAPSDVFGAGK
CCCEEEECCCHHHHHHCCCCCEECCCCCCCCCCCCCCCCEEEEEHHHEECCCCCCCCCCE
VLVFGEVIDKDGTPYSADIRGVLKAYAQSLYEKQGYTLNAANEIEGFLFNGSDAERRYHE
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCHHHHHHHH
TAKFDYVNTGGYYHSLPGDPLRTFIDSCAEVQRAMGFQNEKDHPEVAPSQFEINYSYAEV
HHCCCEEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHH
VAAADQIQLYKLICRQVATRMGLTASFLPKPVVGVNGSGMHTNVSITKGGKNIFWDPKGE
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEEEEEECCCEEEECCCCH
EKMSKFAWQFVDRILTHGNDLCLLLNASVNAYRRLDPHFEAPNQIKASAVDRGSMIRIPI
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCCCHHHHHCCCCCEEEEEC
GNERSSRVEVRSVGPDANPYMVMLGVFRTGLEGEISRIKNLRGAERYLPDNIYDAMANFK
CCCCCCEEEEEECCCCCCCCEEEEEHHHCCCCHHHHHHHHCCCHHHHCCHHHHHHHHCCC
NAEWTTKLLGEDVKGRYADLKQASADRCPRLLGTFVKTPEVQYHHEVYNQFLWNMF
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PNQLQDFLTLSYAELEELNLQAKAERLGRAPTHQIQEQRLKYLTDEKRIKAVTVLFSDL
CCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHC
EGRLHMLDYDKKFLVKSWDNLTFDGSSIRGFTAQRESDLRLSMDWSSFYWAPSDVFGAGK
CCCEEEECCCHHHHHHCCCCCEECCCCCCCCCCCCCCCCEEEEEHHHEECCCCCCCCCCE
VLVFGEVIDKDGTPYSADIRGVLKAYAQSLYEKQGYTLNAANEIEGFLFNGSDAERRYHE
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCHHHHHHHH
TAKFDYVNTGGYYHSLPGDPLRTFIDSCAEVQRAMGFQNEKDHPEVAPSQFEINYSYAEV
HHCCCEEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHH
VAAADQIQLYKLICRQVATRMGLTASFLPKPVVGVNGSGMHTNVSITKGGKNIFWDPKGE
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEEEEEECCCEEEECCCCH
EKMSKFAWQFVDRILTHGNDLCLLLNASVNAYRRLDPHFEAPNQIKASAVDRGSMIRIPI
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCCCHHHHHCCCCCEEEEEC
GNERSSRVEVRSVGPDANPYMVMLGVFRTGLEGEISRIKNLRGAERYLPDNIYDAMANFK
CCCCCCEEEEEECCCCCCCCEEEEEHHHCCCCHHHHHHHHCCCHHHHCCHHHHHHHHCCC
NAEWTTKLLGEDVKGRYADLKQASADRCPRLLGTFVKTPEVQYHHEVYNQFLWNMF
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]