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The map label for this gene is proS

Identifier: 116620962

GI number: 116620962

Start: 2294939

End: 2296381

Strand: Reverse

Name: proS

Synonym: Acid_1843

Alternate gene names: 116620962

Gene position: 2296381-2294939 (Counterclockwise)

Preceding gene: 116620963

Following gene: 116620960

Centisome position: 23.04

GC content: 61.75

Gene sequence:

>1443_bases
ATGGCTGAATCACCGATAACTGCACGAAGCCAGGACTTTGCCGCCTGGTATCAGGACGTTGTTCTGCAAGGCGATATGGC
GGAGCCCGCCGAAATCGTCAAAGGCTGCATGGTCATCAAGGCCAATGGCTATGCGGTCTGGGAAGTGCTGCAGCGCGAGC
TGGACGATCGCTTCAAAGCGACCAATCACCAGAACGTGTATTTCCCGCTGCTGATTCCGCAGAGTTTCCTGAAGAAGGAA
GCGGAGCACGTGGAAGGCTTCGCGCCGGAGCTGGCGGTGGTCACGGTCGCCGGCGGCAAGGAACTGGAGGAGCCGTACGT
AATCCGTCCGACGTCGGAGACCATCATCGGCCACTTCTTCTCCAAGTGGATTCACAGCTACCGCGACCTGCCGCTGCTGT
ACAACCAGTGGGCCAACGTAATCCGCTGGGAGCTGCGCACGCGCATGTTCCTGCGCACCACGGAATTCCTCTGGCAGGAA
GGCCACACCGCGCACTCCAACCACGAGGAAGCCGTGGAAGAAGTGCTGCGGATCCTCGATCTCTACGCCCAGGTCGCCGA
GGATATCATGGCGATGCCGGTGATCAAAGGGGTGAAGACGCACGCGGAGAAATTCGCCGGCGCGCTCAAGAGCTATTCGA
TCGAGGCGATGATGCAGAACGGCCTCGCACTGCAGGCCGGCACGAGCCACGACCTGGGACAGAATTTCGGCAAGGCCTTC
GACGTGCAGTTCCAGAGCAAGGAAGGCCAGCTGGAATACGTCTGGCAGACCAGCTGGGGAGTCAGCACGCGGTTGATCGG
CGGCTTGATCATGACGCACTCCGACGATAAGGGGCTGGTACTGCCGCCCAAGCTCGCGCCGGTGAAGTCCGTGATCGTGC
CGATCTATCGCAAGGAGGAGGAAAGGGCCGCGGTGTTGGAGGCTGCGCACCGCATCGCCAAGGAGATCGGCGCCAAGGTG
GACGATCGCGAGGGCCAATCGCCGGGCGCGAAGTTCTTCCACTGGGAGCGTCGCGGTGTGCCGGTGGTGCTGGAGCTCGG
CCCGCGCGACCTGGCGAGCGGCAAGATCGTGCTCAAGCGGCGTGACACGGGGACCAAGGAGCCGATCGACCAGAGCGAAG
TCGGCGCGAAGGTGGCGGCGGCGCTCGAAGACATGCAGAAGGGCCTGTACACGCGCGCCAAGGATCGCATGAAGGCCAAC
ACGGTGCTGGCGAATTCCATCGAGGAAGTGGAAGCGATCCTCAAAGACGCGACCGCGGAAAAGGGCGGCGGCAAATTCGT
GATGGCGCACATTAAGGATGAGCCGGCCTGCGACGCTCGTTTGAAGGAATTCAAGGCCACGGTCCGCAACATCCCGCTGG
TAGATGAATACGATGGGCCGGGCAAATGCATCGTCAGCGGCGAGACAATCGAAAAGCGCGCCGTGATCGCGAAGGCCTAC
TAA

Upstream 100 bases:

>100_bases
ATCCGCGAAAAGTACACGGTGGAAAAGAAGTCCGAAGCACGCCCCAATTAACCGTAAGAAACATCGTGCCGGTTATGCCA
AACTGGTGATTACACCTCAT

Downstream 100 bases:

>100_bases
GACCGCGAGATAAAAAGCGAATTAGCAACCGCTCGCTAACGCGCGCGGCTCGGATCGGAGCCGCGACCGTGAGGGAGCGG
TTGCTGATTTTAGAACCTGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MAESPITARSQDFAAWYQDVVLQGDMAEPAEIVKGCMVIKANGYAVWEVLQRELDDRFKATNHQNVYFPLLIPQSFLKKE
AEHVEGFAPELAVVTVAGGKELEEPYVIRPTSETIIGHFFSKWIHSYRDLPLLYNQWANVIRWELRTRMFLRTTEFLWQE
GHTAHSNHEEAVEEVLRILDLYAQVAEDIMAMPVIKGVKTHAEKFAGALKSYSIEAMMQNGLALQAGTSHDLGQNFGKAF
DVQFQSKEGQLEYVWQTSWGVSTRLIGGLIMTHSDDKGLVLPPKLAPVKSVIVPIYRKEEERAAVLEAAHRIAKEIGAKV
DDREGQSPGAKFFHWERRGVPVVLELGPRDLASGKIVLKRRDTGTKEPIDQSEVGAKVAAALEDMQKGLYTRAKDRMKAN
TVLANSIEEVEAILKDATAEKGGGKFVMAHIKDEPACDARLKEFKATVRNIPLVDEYDGPGKCIVSGETIEKRAVIAKAY

Sequences:

>Translated_480_residues
MAESPITARSQDFAAWYQDVVLQGDMAEPAEIVKGCMVIKANGYAVWEVLQRELDDRFKATNHQNVYFPLLIPQSFLKKE
AEHVEGFAPELAVVTVAGGKELEEPYVIRPTSETIIGHFFSKWIHSYRDLPLLYNQWANVIRWELRTRMFLRTTEFLWQE
GHTAHSNHEEAVEEVLRILDLYAQVAEDIMAMPVIKGVKTHAEKFAGALKSYSIEAMMQNGLALQAGTSHDLGQNFGKAF
DVQFQSKEGQLEYVWQTSWGVSTRLIGGLIMTHSDDKGLVLPPKLAPVKSVIVPIYRKEEERAAVLEAAHRIAKEIGAKV
DDREGQSPGAKFFHWERRGVPVVLELGPRDLASGKIVLKRRDTGTKEPIDQSEVGAKVAAALEDMQKGLYTRAKDRMKAN
TVLANSIEEVEAILKDATAEKGGGKFVMAHIKDEPACDARLKEFKATVRNIPLVDEYDGPGKCIVSGETIEKRAVIAKAY
>Mature_479_residues
AESPITARSQDFAAWYQDVVLQGDMAEPAEIVKGCMVIKANGYAVWEVLQRELDDRFKATNHQNVYFPLLIPQSFLKKEA
EHVEGFAPELAVVTVAGGKELEEPYVIRPTSETIIGHFFSKWIHSYRDLPLLYNQWANVIRWELRTRMFLRTTEFLWQEG
HTAHSNHEEAVEEVLRILDLYAQVAEDIMAMPVIKGVKTHAEKFAGALKSYSIEAMMQNGLALQAGTSHDLGQNFGKAFD
VQFQSKEGQLEYVWQTSWGVSTRLIGGLIMTHSDDKGLVLPPKLAPVKSVIVPIYRKEEERAAVLEAAHRIAKEIGAKVD
DREGQSPGAKFFHWERRGVPVVLELGPRDLASGKIVLKRRDTGTKEPIDQSEVGAKVAAALEDMQKGLYTRAKDRMKANT
VLANSIEEVEAILKDATAEKGGGKFVMAHIKDEPACDARLKEFKATVRNIPLVDEYDGPGKCIVSGETIEKRAVIAKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=455, Percent_Identity=43.956043956044, Blast_Score=383, Evalue=1e-106,
Organism=Escherichia coli, GI1786392, Length=158, Percent_Identity=27.2151898734177, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71991342, Length=491, Percent_Identity=37.8818737270876, Blast_Score=357, Evalue=6e-99,
Organism=Caenorhabditis elegans, GI71991346, Length=358, Percent_Identity=45.2513966480447, Blast_Score=339, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6321808, Length=411, Percent_Identity=43.5523114355231, Blast_Score=366, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24649468, Length=405, Percent_Identity=42.962962962963, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24649466, Length=405, Percent_Identity=42.962962962963, Blast_Score=349, Evalue=2e-96,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_SOLUE (Q027I0)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_823118.1
- ProteinModelPortal:   Q027I0
- SMR:   Q027I0
- STRING:   Q027I0
- GeneID:   4429989
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_1843
- NMPDR:   fig|234267.9.peg.1756
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   HEAYEDL
- PhylomeDB:   Q027I0
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 53642; Mature: 53511

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESPITARSQDFAAWYQDVVLQGDMAEPAEIVKGCMVIKANGYAVWEVLQRELDDRFKA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHC
TNHQNVYFPLLIPQSFLKKEAEHVEGFAPELAVVTVAGGKELEEPYVIRPTSETIIGHFF
CCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHH
SKWIHSYRDLPLLYNQWANVIRWELRTRMFLRTTEFLWQEGHTAHSNHEEAVEEVLRILD
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LYAQVAEDIMAMPVIKGVKTHAEKFAGALKSYSIEAMMQNGLALQAGTSHDLGQNFGKAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHCCCEE
DVQFQSKEGQLEYVWQTSWGVSTRLIGGLIMTHSDDKGLVLPPKLAPVKSVIVPIYRKEE
EEEEECCCCCEEEEEECCCCCHHHHHHCEEEEECCCCCCEECCCCCCHHHHHHHHHCCCH
ERAAVLEAAHRIAKEIGAKVDDREGQSPGAKFFHWERRGVPVVLELGPRDLASGKIVLKR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCEEEEEE
RDTGTKEPIDQSEVGAKVAAALEDMQKGLYTRAKDRMKANTVLANSIEEVEAILKDATAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KGGGKFVMAHIKDEPACDARLKEFKATVRNIPLVDEYDGPGKCIVSGETIEKRAVIAKAY
CCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCEEEECCCHHHHEEEEECC
>Mature Secondary Structure 
AESPITARSQDFAAWYQDVVLQGDMAEPAEIVKGCMVIKANGYAVWEVLQRELDDRFKA
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHC
TNHQNVYFPLLIPQSFLKKEAEHVEGFAPELAVVTVAGGKELEEPYVIRPTSETIIGHFF
CCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHH
SKWIHSYRDLPLLYNQWANVIRWELRTRMFLRTTEFLWQEGHTAHSNHEEAVEEVLRILD
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LYAQVAEDIMAMPVIKGVKTHAEKFAGALKSYSIEAMMQNGLALQAGTSHDLGQNFGKAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHCCCEE
DVQFQSKEGQLEYVWQTSWGVSTRLIGGLIMTHSDDKGLVLPPKLAPVKSVIVPIYRKEE
EEEEECCCCCEEEEEECCCCCHHHHHHCEEEEECCCCCCEECCCCCCHHHHHHHHHCCCH
ERAAVLEAAHRIAKEIGAKVDDREGQSPGAKFFHWERRGVPVVLELGPRDLASGKIVLKR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCEEEEEE
RDTGTKEPIDQSEVGAKVAAALEDMQKGLYTRAKDRMKANTVLANSIEEVEAILKDATAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KGGGKFVMAHIKDEPACDARLKEFKATVRNIPLVDEYDGPGKCIVSGETIEKRAVIAKAY
CCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCEEEECCCHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA