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The map label for this gene is hsdR [H]

Identifier: 116620817

GI number: 116620817

Start: 2116466

End: 2118178

Strand: Reverse

Name: hsdR [H]

Synonym: Acid_1698

Alternate gene names: 116620817

Gene position: 2118178-2116466 (Counterclockwise)

Preceding gene: 116620818

Following gene: 116620816

Centisome position: 21.25

GC content: 58.49

Gene sequence:

>1713_bases
ATGCACCGCGGTTTCTTATCAAACGACGTTCTGGCGAACTGCAGCTGGCAATACCTCGAAAGATCAGTCGGCCGGCTGCT
ATTGAGTGAAGGATTCAGCGGAGTGCGGATGTTGGGACAGAGTGGGGACGAGGGCGCCGACCTCTTGGCTCACAGAAATG
GGAAGCGGTGGCTGGTTCAGGTCAAGCGCCGCCGTTCCACCAACATTGGGGTTGAAGTACTGGATGAGATCATTCGGGCC
GCGCACATTTACCGCGCTGACATTCCAGTAGTCGCGACAAATCTCGGTTTCACTGACCAGGCGCGCACTCAGCAATCAAT
GCTGATGGCGGCGGGGACCCCGCTCCAGCTTTGGGACCGTACCACGCTCTCCAGGAGGTGGGAGAAGCTAGCAGATCGGC
CAGTTGCAACGAGACAGCTGCGCGGCTATCAGGAGAGCGCTATCGCATCGATTCTCGAGGTCGCTGTTTCAGGCAAACGG
CGCTCCGCTCTGGTTGTTATGGCCACGGGGCTCGGGAAGACCTTCGTTGCGGCAGAGTCTTACCGACGCATGCGGGAGAC
GCACCCCACCTTGCGCGCGCTTGTCCTGGCACACACGAATGACCTCGTCTACCAACTGGAGCGGGCTTTCTGGCCATGTC
TCGACAAGAGCGAAATCACCGGTGTCTGGAACGGCTACGAGGTCCCCGATCCTGAAGCGAGCTTGACCTTCGCATGTATT
GACTCCGTCGCCTCCAAGCTTCAAGTGTCTGGACGTCTCGACCTGGGCGTCGATCTCGTAATCGTCGACGAATGCCACCA
TGCGGGAAGCGACGAGTATCGAGTGGTCTTGGAGGCCCTGCGATGCGGCTCGCAAGATGGACCTTTCCTGATTGGGATGA
CGGCTACCCCGTGGCGCCCCGACGGCACCAGCATCCACTCCCTTTTTGGTGAGCCAGTATCTTGTGTTGATATCGTGCAA
GGGCTCAAGATGGGGTGGCTTTCAAATGTGGATTACCGCATGCACGTTGACAACATTAACTGGCAGGAGCTCAACCGGGT
GCATCATCTCACGCCACGAGGGCTCAACCGGACGATCTTCATCCAGGAGTGGGATGACGCGGTGGTCCAAAAGCTCAAGG
AAACCTGGTACGAAATCCCTCATGCGCGGGCCATCGTTTTCTGCGGCAACATCGACCATGCGATCACAATGCGCGATCGC
GTCAACGGAGTAGGCTTTGCCAGGGCGGAGGCCTTGTACGCAGGTAGCCCGGGCGCTCCAAGACTCAGTCATGCGCAGCG
GTCGAAAATCCTCGCTGATTTTCACGACGGACACGCCGGCGTGTTGTGCGCCGTGGATCTCCTCAATGAGGGGATTGATC
TTCCCGACGTGAACATCTGCGTGTTCCAGAGAGTGACGCACAGCCGCAGGATCTTTGTACAGCAGCTGGGACGGGGCCTG
AGACTAGCATCTGGGAAAGACCGGGTGATCGTCCTCGACTTCGTGTCAGATGTCCGTCGGTTTGCCGCAGGGCTGGACCT
GCAGAACAAGTTGCAGGAGGGACCCCAGTACGTCTCCCTCGGCAGCAAGGTCCGCTTCATGAACGTGGGGGGCGAAGACA
AGGAAGCCGAATCCTTCTTGCGAGCCTGGCTCCAGGATGTGGCCGCTATCGAGTCCGCAGGTGAAGATGACCACGTACTC
AAGTTTCCGCCGTCGCTTTCAGATAGATTATAG

Upstream 100 bases:

>100_bases
GTTTCTTGGACACGCGACAGTCTGATCCGGGTTACGTTGTTCGTGTCGAGAGAACGCTGCATCTGCTGTTCCGAAACCTG
AGGCCAGGACTCTAAAACGG

Downstream 100 bases:

>100_bases
GGATGGATTGGAACCAAGTCAGGACTTCCTTGAAACAGATGGAAGGCCGGCTTCTAAGCTCGATCTCGGGCAAGAGCGAT
ATTAGAATTGCCGAGATCGA

Product: type III restriction enzyme, res subunit

Products: NA

Alternate protein names: R.EcoEI [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MHRGFLSNDVLANCSWQYLERSVGRLLLSEGFSGVRMLGQSGDEGADLLAHRNGKRWLVQVKRRRSTNIGVEVLDEIIRA
AHIYRADIPVVATNLGFTDQARTQQSMLMAAGTPLQLWDRTTLSRRWEKLADRPVATRQLRGYQESAIASILEVAVSGKR
RSALVVMATGLGKTFVAAESYRRMRETHPTLRALVLAHTNDLVYQLERAFWPCLDKSEITGVWNGYEVPDPEASLTFACI
DSVASKLQVSGRLDLGVDLVIVDECHHAGSDEYRVVLEALRCGSQDGPFLIGMTATPWRPDGTSIHSLFGEPVSCVDIVQ
GLKMGWLSNVDYRMHVDNINWQELNRVHHLTPRGLNRTIFIQEWDDAVVQKLKETWYEIPHARAIVFCGNIDHAITMRDR
VNGVGFARAEALYAGSPGAPRLSHAQRSKILADFHDGHAGVLCAVDLLNEGIDLPDVNICVFQRVTHSRRIFVQQLGRGL
RLASGKDRVIVLDFVSDVRRFAAGLDLQNKLQEGPQYVSLGSKVRFMNVGGEDKEAESFLRAWLQDVAAIESAGEDDHVL
KFPPSLSDRL

Sequences:

>Translated_570_residues
MHRGFLSNDVLANCSWQYLERSVGRLLLSEGFSGVRMLGQSGDEGADLLAHRNGKRWLVQVKRRRSTNIGVEVLDEIIRA
AHIYRADIPVVATNLGFTDQARTQQSMLMAAGTPLQLWDRTTLSRRWEKLADRPVATRQLRGYQESAIASILEVAVSGKR
RSALVVMATGLGKTFVAAESYRRMRETHPTLRALVLAHTNDLVYQLERAFWPCLDKSEITGVWNGYEVPDPEASLTFACI
DSVASKLQVSGRLDLGVDLVIVDECHHAGSDEYRVVLEALRCGSQDGPFLIGMTATPWRPDGTSIHSLFGEPVSCVDIVQ
GLKMGWLSNVDYRMHVDNINWQELNRVHHLTPRGLNRTIFIQEWDDAVVQKLKETWYEIPHARAIVFCGNIDHAITMRDR
VNGVGFARAEALYAGSPGAPRLSHAQRSKILADFHDGHAGVLCAVDLLNEGIDLPDVNICVFQRVTHSRRIFVQQLGRGL
RLASGKDRVIVLDFVSDVRRFAAGLDLQNKLQEGPQYVSLGSKVRFMNVGGEDKEAESFLRAWLQDVAAIESAGEDDHVL
KFPPSLSDRL
>Mature_570_residues
MHRGFLSNDVLANCSWQYLERSVGRLLLSEGFSGVRMLGQSGDEGADLLAHRNGKRWLVQVKRRRSTNIGVEVLDEIIRA
AHIYRADIPVVATNLGFTDQARTQQSMLMAAGTPLQLWDRTTLSRRWEKLADRPVATRQLRGYQESAIASILEVAVSGKR
RSALVVMATGLGKTFVAAESYRRMRETHPTLRALVLAHTNDLVYQLERAFWPCLDKSEITGVWNGYEVPDPEASLTFACI
DSVASKLQVSGRLDLGVDLVIVDECHHAGSDEYRVVLEALRCGSQDGPFLIGMTATPWRPDGTSIHSLFGEPVSCVDIVQ
GLKMGWLSNVDYRMHVDNINWQELNRVHHLTPRGLNRTIFIQEWDDAVVQKLKETWYEIPHARAIVFCGNIDHAITMRDR
VNGVGFARAEALYAGSPGAPRLSHAQRSKILADFHDGHAGVLCAVDLLNEGIDLPDVNICVFQRVTHSRRIFVQQLGRGL
RLASGKDRVIVLDFVSDVRRFAAGLDLQNKLQEGPQYVSLGSKVRFMNVGGEDKEAESFLRAWLQDVAAIESAGEDDHVL
KFPPSLSDRL

Specific function: The EcoEI enzyme recognizes 5'-GAGN(7)ATGC-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG1061

COG function: function code KL; DNA or RNA helicases of superfamily II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788511, Length=378, Percent_Identity=26.7195767195767, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR013670
- InterPro:   IPR007409 [H]

Pfam domain/function: PF08463 EcoEI_R_C; PF04313 HSDR_N; PF04851 ResIII [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 63591; Mature: 63591

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRGFLSNDVLANCSWQYLERSVGRLLLSEGFSGVRMLGQSGDEGADLLAHRNGKRWLVQ
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHCCCCHHHHH
VKRRRSTNIGVEVLDEIIRAAHIYRADIPVVATNLGFTDQARTQQSMLMAAGTPLQLWDR
HHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHH
TTLSRRWEKLADRPVATRQLRGYQESAIASILEVAVSGKRRSALVVMATGLGKTFVAAES
HHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
YRRMRETHPTLRALVLAHTNDLVYQLERAFWPCLDKSEITGVWNGYEVPDPEASLTFACI
HHHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
DSVASKLQVSGRLDLGVDLVIVDECHHAGSDEYRVVLEALRCGSQDGPFLIGMTATPWRP
HHHHHHHEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
DGTSIHSLFGEPVSCVDIVQGLKMGWLSNVDYRMHVDNINWQELNRVHHLTPRGLNRTIF
CCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEE
IQEWDDAVVQKLKETWYEIPHARAIVFCGNIDHAITMRDRVNGVGFARAEALYAGSPGAP
EEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEHHHCCCCCHHHHHEEECCCCCCC
RLSHAQRSKILADFHDGHAGVLCAVDLLNEGIDLPDVNICVFQRVTHSRRIFVQQLGRGL
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RLASGKDRVIVLDFVSDVRRFAAGLDLQNKLQEGPQYVSLGSKVRFMNVGGEDKEAESFL
EECCCCCCEEEEHHHHHHHHHHHCCCHHHHHHCCCCEEECCCEEEEEECCCCCHHHHHHH
RAWLQDVAAIESAGEDDHVLKFPPSLSDRL
HHHHHHHHHHHHCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MHRGFLSNDVLANCSWQYLERSVGRLLLSEGFSGVRMLGQSGDEGADLLAHRNGKRWLVQ
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHCCCCHHHHH
VKRRRSTNIGVEVLDEIIRAAHIYRADIPVVATNLGFTDQARTQQSMLMAAGTPLQLWDR
HHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHH
TTLSRRWEKLADRPVATRQLRGYQESAIASILEVAVSGKRRSALVVMATGLGKTFVAAES
HHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
YRRMRETHPTLRALVLAHTNDLVYQLERAFWPCLDKSEITGVWNGYEVPDPEASLTFACI
HHHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
DSVASKLQVSGRLDLGVDLVIVDECHHAGSDEYRVVLEALRCGSQDGPFLIGMTATPWRP
HHHHHHHEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
DGTSIHSLFGEPVSCVDIVQGLKMGWLSNVDYRMHVDNINWQELNRVHHLTPRGLNRTIF
CCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEE
IQEWDDAVVQKLKETWYEIPHARAIVFCGNIDHAITMRDRVNGVGFARAEALYAGSPGAP
EEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEHHHCCCCCHHHHHEEECCCCCCC
RLSHAQRSKILADFHDGHAGVLCAVDLLNEGIDLPDVNICVFQRVTHSRRIFVQQLGRGL
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RLASGKDRVIVLDFVSDVRRFAAGLDLQNKLQEGPQYVSLGSKVRFMNVGGEDKEAESFL
EECCCCCCEEEEHHHHHHHHHHHCCCHHHHHHCCCCEEECCCEEEEEECCCCCHHHHHHH
RAWLQDVAAIESAGEDDHVLKFPPSLSDRL
HHHHHHHHHHHHCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]