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The map label for this gene is mutY [C]

Identifier: 116620815

GI number: 116620815

Start: 2115041

End: 2115700

Strand: Reverse

Name: mutY [C]

Synonym: Acid_1696

Alternate gene names: 116620815

Gene position: 2115700-2115041 (Counterclockwise)

Preceding gene: 116620816

Following gene: 116620808

Centisome position: 21.23

GC content: 60.76

Gene sequence:

>660_bases
ATGGAATCTCACCAGCGTGCGACGCTCGTTCGTCGCCTGCTCCTGAAATGGTTCCGTAGTTCCGGCCGCAGTTTCTATTG
GCGCGAGAATCGCGATCCCTACGTCGTTCTGGTCTCGGAATTGCTGCTCAAGAAAACCGCTGCGCCAGTCGTAGATAGAT
TCTTACCCGCATTCCTAAAACGCTTCCCAGACTTCGCGAGCCTTTCCCGCGCGCGTCACGCCACACTGGTGAGGATCCTG
CAGCCGCTCGGACTCTCTGATCAGCGCGCAGGGCAGCTTCGAGCACTCGCCCAGGCAATCAGCGGGTCAAAGGATGGATG
CGTCCCGGCGACGCGGCAGGACCTTTTGGCGCTGCCCGGAGTTGGCGAATATACCGCGAACTCGCTCCTGTGTGTTTCAT
TTGGCCAGGCAGTGCCAGTCGTCGATACGAATGTCGCCAGGATCGTGATGCGAGCATTCGGGATTGGCCACAGCCGTTGT
GAAGCGCGCAGAAGCCCGGAAATCTGGGGACTGGCGGCTGACATCACGGGAAATGTCCCCACAAGGGCCGTCGAGGTCAA
CTGGGCACTGCTCGATCTGGGCGCCAACGTCTGCACGGCGCGGACACCGCGGTGCAGGGACTGCCCTGTCAGCTCGATCT
GCCGCTCATCAAACACCTGA

Upstream 100 bases:

>100_bases
GATCCTCGGTCGGAACAGGATACCCTCACGCTCGGTGGCCCTGTAGTCGTGGTTCGCTTTTCAGATGGCGCGAAACTTGC
ATTCGGACAATGATCAGGCT

Downstream 100 bases:

>100_bases
CACGACCGACCCGCCGATGACGGCGCTCCCTCCGGCCGGAGTCCAGAAGACAAGGTTCTCAGATTTGCCGCCAGTTAAAG
GGCGTGGACGGCTTCTTTCT

Product: helix-hairpin-helix DNA-binding motif-containing protein

Products: NA

Alternate protein names: Mismatch glycosylase; MIG; Thymine-DNA glycosylase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MESHQRATLVRRLLLKWFRSSGRSFYWRENRDPYVVLVSELLLKKTAAPVVDRFLPAFLKRFPDFASLSRARHATLVRIL
QPLGLSDQRAGQLRALAQAISGSKDGCVPATRQDLLALPGVGEYTANSLLCVSFGQAVPVVDTNVARIVMRAFGIGHSRC
EARRSPEIWGLAADITGNVPTRAVEVNWALLDLGANVCTARTPRCRDCPVSSICRSSNT

Sequences:

>Translated_219_residues
MESHQRATLVRRLLLKWFRSSGRSFYWRENRDPYVVLVSELLLKKTAAPVVDRFLPAFLKRFPDFASLSRARHATLVRIL
QPLGLSDQRAGQLRALAQAISGSKDGCVPATRQDLLALPGVGEYTANSLLCVSFGQAVPVVDTNVARIVMRAFGIGHSRC
EARRSPEIWGLAADITGNVPTRAVEVNWALLDLGANVCTARTPRCRDCPVSSICRSSNT
>Mature_219_residues
MESHQRATLVRRLLLKWFRSSGRSFYWRENRDPYVVLVSELLLKKTAAPVVDRFLPAFLKRFPDFASLSRARHATLVRIL
QPLGLSDQRAGQLRALAQAISGSKDGCVPATRQDLLALPGVGEYTANSLLCVSFGQAVPVVDTNVARIVMRAFGIGHSRC
EARRSPEIWGLAADITGNVPTRAVEVNWALLDLGANVCTARTPRCRDCPVSSICRSSNT

Specific function: Could act in DNA repair. Restriction methylase M.mthtI, which is encoded by this plasmid, generates 5-methylcytosine which is, especially under thermophilic conditions, subject to deamination resulting in G-T mismatches. This protein could correct these m

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298654, Length=218, Percent_Identity=33.9449541284404, Blast_Score=120, Evalue=7e-28,
Organism=Homo sapiens, GI115298652, Length=218, Percent_Identity=33.9449541284404, Blast_Score=120, Evalue=7e-28,
Organism=Homo sapiens, GI115298650, Length=218, Percent_Identity=33.9449541284404, Blast_Score=120, Evalue=8e-28,
Organism=Homo sapiens, GI115298648, Length=218, Percent_Identity=33.9449541284404, Blast_Score=120, Evalue=8e-28,
Organism=Homo sapiens, GI6912520, Length=218, Percent_Identity=33.9449541284404, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI190358497, Length=218, Percent_Identity=33.9449541284404, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1789331, Length=203, Percent_Identity=32.0197044334975, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 24131; Mature: 24131

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESHQRATLVRRLLLKWFRSSGRSFYWRENRDPYVVLVSELLLKKTAAPVVDRFLPAFLK
CCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
RFPDFASLSRARHATLVRILQPLGLSDQRAGQLRALAQAISGSKDGCVPATRQDLLALPG
HCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC
VGEYTANSLLCVSFGQAVPVVDTNVARIVMRAFGIGHSRCEARRSPEIWGLAADITGNVP
CCCHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCC
TRAVEVNWALLDLGANVCTARTPRCRDCPVSSICRSSNT
CEEEEEEEHHEECCCCHHCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MESHQRATLVRRLLLKWFRSSGRSFYWRENRDPYVVLVSELLLKKTAAPVVDRFLPAFLK
CCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
RFPDFASLSRARHATLVRILQPLGLSDQRAGQLRALAQAISGSKDGCVPATRQDLLALPG
HCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC
VGEYTANSLLCVSFGQAVPVVDTNVARIVMRAFGIGHSRCEARRSPEIWGLAADITGNVP
CCCHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCC
TRAVEVNWALLDLGANVCTARTPRCRDCPVSSICRSSNT
CEEEEEEEHHEECCCCHHCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1336177; 11786018 [H]