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The map label for this gene is metK [H]

Identifier: 116620805

GI number: 116620805

Start: 2098476

End: 2099570

Strand: Direct

Name: metK [H]

Synonym: Acid_1686

Alternate gene names: 116620805

Gene position: 2098476-2099570 (Clockwise)

Preceding gene: 116620804

Following gene: 116620809

Centisome position: 21.06

GC content: 64.57

Gene sequence:

>1095_bases
ATGCTTTACGCAGCCGAATATGTGCTTCCGGGTCACCCGGACAAGTTGTGCGACGCGATCGCGGACGCTCTGGTCGAAGA
GGCAGTGCGGCACGAAAAGCGGGCCCTGTGCGCGATCGAGGTGGCAGTTCACCGCGCATCGGTTTTCGTCACCGGGCGAA
TCGCGTGCCGGGGAGCCAGACGAATCCCGGTCAAGGATATCGTGCGCGAGTGTTATCGCGTGGCAGGCTATCGCACGGAG
TGGCGGCCGTCGCCCGAAGAACTCGACGTGCGCACGAACCTGTGCCTGGGCCCTCTGGTGGAGGGTGAAGCGGGCTTCCG
CGAAGTCGCCGACGACCAGTCGATCATCACCGGGTACGCAATCGATTCGCCTGGCACGAACTTTTTGCCCCCCGAACACT
GGCTGGCTTCGCGTCTGGCACGGCGGTTGGAGACGCTACGAACCGGGCGGCCGGACCTGCGGCTCGGACCGGACGGGAAA
CTCGCGCTGGTTTTCGATGCAGGATCGAAAGCGCTCGCCTGCTTGACCGCGTCGGTGCAGCAGACCGTCGTCGGCGACGA
GATCGAGTTGAACCGCGCCGTCCGTCAGTGCGTCGCGGAGGAACTGGCAAGCTCCGTCGCGGCGATTTCCGGGTTGCAGG
CTGAAATACCGGAATCATTCCACGTGAACGGCGCCGGCAACTTCGAAGTCGGCGGTCCAGAAGGGGACAACGGGCTGAGC
GGGAAGAAACTGGTCGTGGATGGCTACGGTCCGCGCGTCCCGATCGGAGGTGGTGCACTCAGCGGGAAGGACTTCTTCAA
GGCGGACCGCGCCGGCGCCATTCTCGCCCGGCGGCTCGCCAAGGCGGTTGTGATGAGCGGCGCCGCGCCGGAGTGCACAG
CCACGATCACAATTGCACCAGGCGAACGCGCCTTCCGAATCGTGTCGCTCGTGGGCTGTGGTGCCGCTCTCGATCCGCGT
CGATGGACCGGGTTGATGGACCTCTCGCTGATGAAGGCTGGTGAGAACTACGCCGGAATGACGGGACTCCCGGATCTCGC
CCGTTACGGACACTTCACCTTACCGGACCGACCGTGGGAGAGAATCGAGTTCTGA

Upstream 100 bases:

>100_bases
CGAGCGTGCGCCTGGGCGTCGCGTTGACGCCTGCCGCCAATCGCAGCGACCTGGAGAAGTTCGTCCGCCACTGGGCGGAA
CTCGAAACGGAGAATTGACG

Downstream 100 bases:

>100_bases
TCCTGACGAAGAATCGCGAAGAGCTGAACGCCATTCTCACGGCCGATCAAATTCGCTATCGACAACGCATGAAGAAGTGC
GCCGGATCAAAAGAGCCCCA

Product: methionine adenosyltransferase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MLYAAEYVLPGHPDKLCDAIADALVEEAVRHEKRALCAIEVAVHRASVFVTGRIACRGARRIPVKDIVRECYRVAGYRTE
WRPSPEELDVRTNLCLGPLVEGEAGFREVADDQSIITGYAIDSPGTNFLPPEHWLASRLARRLETLRTGRPDLRLGPDGK
LALVFDAGSKALACLTASVQQTVVGDEIELNRAVRQCVAEELASSVAAISGLQAEIPESFHVNGAGNFEVGGPEGDNGLS
GKKLVVDGYGPRVPIGGGALSGKDFFKADRAGAILARRLAKAVVMSGAAPECTATITIAPGERAFRIVSLVGCGAALDPR
RWTGLMDLSLMKAGENYAGMTGLPDLARYGHFTLPDRPWERIEF

Sequences:

>Translated_364_residues
MLYAAEYVLPGHPDKLCDAIADALVEEAVRHEKRALCAIEVAVHRASVFVTGRIACRGARRIPVKDIVRECYRVAGYRTE
WRPSPEELDVRTNLCLGPLVEGEAGFREVADDQSIITGYAIDSPGTNFLPPEHWLASRLARRLETLRTGRPDLRLGPDGK
LALVFDAGSKALACLTASVQQTVVGDEIELNRAVRQCVAEELASSVAAISGLQAEIPESFHVNGAGNFEVGGPEGDNGLS
GKKLVVDGYGPRVPIGGGALSGKDFFKADRAGAILARRLAKAVVMSGAAPECTATITIAPGERAFRIVSLVGCGAALDPR
RWTGLMDLSLMKAGENYAGMTGLPDLARYGHFTLPDRPWERIEF
>Mature_364_residues
MLYAAEYVLPGHPDKLCDAIADALVEEAVRHEKRALCAIEVAVHRASVFVTGRIACRGARRIPVKDIVRECYRVAGYRTE
WRPSPEELDVRTNLCLGPLVEGEAGFREVADDQSIITGYAIDSPGTNFLPPEHWLASRLARRLETLRTGRPDLRLGPDGK
LALVFDAGSKALACLTASVQQTVVGDEIELNRAVRQCVAEELASSVAAISGLQAEIPESFHVNGAGNFEVGGPEGDNGLS
GKKLVVDGYGPRVPIGGGALSGKDFFKADRAGAILARRLAKAVVMSGAAPECTATITIAPGERAFRIVSLVGCGAALDPR
RWTGLMDLSLMKAGENYAGMTGLPDLARYGHFTLPDRPWERIEF

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=384, Percent_Identity=28.90625, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI4557737, Length=387, Percent_Identity=26.8733850129199, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1789311, Length=382, Percent_Identity=28.0104712041885, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=29.0155440414508, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17538494, Length=388, Percent_Identity=28.6082474226804, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17538498, Length=385, Percent_Identity=28.3116883116883, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=27.5064267352185, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32565905, Length=378, Percent_Identity=27.5132275132275, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32565903, Length=268, Percent_Identity=30.2238805970149, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25145635, Length=273, Percent_Identity=30.03663003663, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32565911, Length=265, Percent_Identity=29.811320754717, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323209, Length=386, Percent_Identity=27.979274611399, Blast_Score=109, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6320710, Length=398, Percent_Identity=27.1356783919598, Blast_Score=105, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24580527, Length=326, Percent_Identity=29.7546012269939, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24580525, Length=326, Percent_Identity=29.7546012269939, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI45552159, Length=326, Percent_Identity=30.0613496932515, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24580529, Length=326, Percent_Identity=30.0613496932515, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24580537, Length=326, Percent_Identity=30.0613496932515, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24580533, Length=326, Percent_Identity=30.0613496932515, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24580535, Length=326, Percent_Identity=30.0613496932515, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24580531, Length=326, Percent_Identity=30.0613496932515, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24580541, Length=313, Percent_Identity=29.073482428115, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 39060; Mature: 39060

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYAAEYVLPGHPDKLCDAIADALVEEAVRHEKRALCAIEVAVHRASVFVTGRIACRGAR
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCC
RIPVKDIVRECYRVAGYRTEWRPSPEELDVRTNLCLGPLVEGEAGFREVADDQSIITGYA
CCCHHHHHHHHHHHCCCCCCCCCCHHHHCHHHCCEECCCCCCCCCHHHHCCCCCEEEEEE
IDSPGTNFLPPEHWLASRLARRLETLRTGRPDLRLGPDGKLALVFDAGSKALACLTASVQ
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEECCCCEEHHHHHHHH
QTVVGDEIELNRAVRQCVAEELASSVAAISGLQAEIPESFHVNGAGNFEVGGPEGDNGLS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEECCCCCCCCCCCCCCCCCC
GKKLVVDGYGPRVPIGGGALSGKDFFKADRAGAILARRLAKAVVMSGAAPECTATITIAP
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
GERAFRIVSLVGCGAALDPRRWTGLMDLSLMKAGENYAGMTGLPDLARYGHFTLPDRPWE
CCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHH
RIEF
HCCC
>Mature Secondary Structure
MLYAAEYVLPGHPDKLCDAIADALVEEAVRHEKRALCAIEVAVHRASVFVTGRIACRGAR
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCC
RIPVKDIVRECYRVAGYRTEWRPSPEELDVRTNLCLGPLVEGEAGFREVADDQSIITGYA
CCCHHHHHHHHHHHCCCCCCCCCCHHHHCHHHCCEECCCCCCCCCHHHHCCCCCEEEEEE
IDSPGTNFLPPEHWLASRLARRLETLRTGRPDLRLGPDGKLALVFDAGSKALACLTASVQ
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEECCCCEEHHHHHHHH
QTVVGDEIELNRAVRQCVAEELASSVAAISGLQAEIPESFHVNGAGNFEVGGPEGDNGLS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEECCCCCCCCCCCCCCCCCC
GKKLVVDGYGPRVPIGGGALSGKDFFKADRAGAILARRLAKAVVMSGAAPECTATITIAP
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
GERAFRIVSLVGCGAALDPRRWTGLMDLSLMKAGENYAGMTGLPDLARYGHFTLPDRPWE
CCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHH
RIEF
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA