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The map label for this gene is gltA [H]

Identifier: 116620772

GI number: 116620772

Start: 2048498

End: 2049808

Strand: Reverse

Name: gltA [H]

Synonym: Acid_1653

Alternate gene names: 116620772

Gene position: 2049808-2048498 (Counterclockwise)

Preceding gene: 116620777

Following gene: 116620771

Centisome position: 20.57

GC content: 57.82

Gene sequence:

>1311_bases
ATGGATAACGAGACGCTGACCATAACGGACAATCGGACGCAACAGACATACACCGTCCCGATCTATCGCGGGACGGTACG
AGCAATGGACCTGCGGCAGATTAAGAAGGATGCCGACGATTTCGGGCTGATGACGTACGACCCGGCGTTTATGAACACGG
CGTCGTGCCAGAGCGCGATCACCTACCTGGATGGCGAGAAAGGAATCCTGCGATATCGCGGATACCCGATCGAGCAACTG
GCGGAGCAGTGCAGCTTCCTGGAAGTCGCGTACCTGCTGTTGAATGGGGAACTGCCTACGGCGGAGCAATTGGAATACTG
GCAGTGCAAGGTGATGCGGCACACGATGACCCATGAAAACGTCAAAAAGTTCATGGACGGCTTCCATTACGATGCGCATC
CAATGGGGATTCTGATCAGCACGGTGGCCGCGCTTTCGACCTTTTATCCCGAGGCGAAGAATGTAGGCGACGCAAAGTGC
CGGCACAAACAGATCGTGCGACTGATCGGCAAGATGCCGACGCTGGCGGCTTACGCGTACCGCCACCGGCTGGGGTTGCC
GTATATCGCTCCGGATCCGGAGTTGGGATATACGAAGAACTACATGAACATGCTGTGGAAGCGGACGGAACCGAAGTACG
CGGCGAATCCAGTGCTGGCGCGGGCGCTGGAAGTACTCTTCATTTTGCACGCGGATCACGAGCAGAATTGCAGCACCAGC
ACGATGCGGGCGGTGGGAAGTTCGAATGCGGACCCGTTTGTGACGACGTCGGCGGCGATTGCCGCGCTATCGGGACCTCT
GCACGGGGGTGCGAACGAAGAAGTGCTGCGTATGCTGGACGAGATCGGCAAACCGGAGAACATTCCGGGGTTCATCGAAC
AGGTGAAGGAAGGCAAGCGCAAGCTCATGGGATTCGGGCACCGGGTCTACAAGAACTACGACCCGCGCGCGCGGATCATC
AAGCAGGTGGCGGAGGAAGTCTTCGAGGTTACGGGGCGCAATCCGAAGATCGATATCGCGCTGGAACTGGAAAAGATCGC
GTTGTCGGACGATTACTTCATTAAGCGCAAGCTGTATCCGAACGTGGATTTCTATTCGGGGATCATTTACCAGGCGATGG
GTTTCACGCCGGACCAGTTTACGGTGCTGTTCGGAATTCCGCGCACGGTGGGATGGCTGGCGCAGTGGCAGGAAATGCAG
CAGGATCCGGACAAGAAGATCGCGCGACCGCGGCAGGTGTATACGGGTGAAGATGAGCGCGACTTCGTGCCGATTGCGGA
TCGCGGAGCGAGGGCGATGACCGCCGTATAA

Upstream 100 bases:

>100_bases
GGAAAGCGACGAAGAAGCCCACAACATGTAGAAAATTCGGACAATCTAGTATGGAATATGACGTGCAGGTTAGCCGCCGA
CGATTCAGGAGGATAGCCTG

Downstream 100 bases:

>100_bases
ATTCCTTGGTGGAAGTAGATCGCTCCCTGGCTCGCTGCAAGGCAGGCTACGGGAGCGATTTTGCTTTTTGGGAACCGGGA
TATCTTTACCGGGGTGCGGA

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MDNETLTITDNRTQQTYTVPIYRGTVRAMDLRQIKKDADDFGLMTYDPAFMNTASCQSAITYLDGEKGILRYRGYPIEQL
AEQCSFLEVAYLLLNGELPTAEQLEYWQCKVMRHTMTHENVKKFMDGFHYDAHPMGILISTVAALSTFYPEAKNVGDAKC
RHKQIVRLIGKMPTLAAYAYRHRLGLPYIAPDPELGYTKNYMNMLWKRTEPKYAANPVLARALEVLFILHADHEQNCSTS
TMRAVGSSNADPFVTTSAAIAALSGPLHGGANEEVLRMLDEIGKPENIPGFIEQVKEGKRKLMGFGHRVYKNYDPRARII
KQVAEEVFEVTGRNPKIDIALELEKIALSDDYFIKRKLYPNVDFYSGIIYQAMGFTPDQFTVLFGIPRTVGWLAQWQEMQ
QDPDKKIARPRQVYTGEDERDFVPIADRGARAMTAV

Sequences:

>Translated_436_residues
MDNETLTITDNRTQQTYTVPIYRGTVRAMDLRQIKKDADDFGLMTYDPAFMNTASCQSAITYLDGEKGILRYRGYPIEQL
AEQCSFLEVAYLLLNGELPTAEQLEYWQCKVMRHTMTHENVKKFMDGFHYDAHPMGILISTVAALSTFYPEAKNVGDAKC
RHKQIVRLIGKMPTLAAYAYRHRLGLPYIAPDPELGYTKNYMNMLWKRTEPKYAANPVLARALEVLFILHADHEQNCSTS
TMRAVGSSNADPFVTTSAAIAALSGPLHGGANEEVLRMLDEIGKPENIPGFIEQVKEGKRKLMGFGHRVYKNYDPRARII
KQVAEEVFEVTGRNPKIDIALELEKIALSDDYFIKRKLYPNVDFYSGIIYQAMGFTPDQFTVLFGIPRTVGWLAQWQEMQ
QDPDKKIARPRQVYTGEDERDFVPIADRGARAMTAV
>Mature_436_residues
MDNETLTITDNRTQQTYTVPIYRGTVRAMDLRQIKKDADDFGLMTYDPAFMNTASCQSAITYLDGEKGILRYRGYPIEQL
AEQCSFLEVAYLLLNGELPTAEQLEYWQCKVMRHTMTHENVKKFMDGFHYDAHPMGILISTVAALSTFYPEAKNVGDAKC
RHKQIVRLIGKMPTLAAYAYRHRLGLPYIAPDPELGYTKNYMNMLWKRTEPKYAANPVLARALEVLFILHADHEQNCSTS
TMRAVGSSNADPFVTTSAAIAALSGPLHGGANEEVLRMLDEIGKPENIPGFIEQVKEGKRKLMGFGHRVYKNYDPRARII
KQVAEEVFEVTGRNPKIDIALELEKIALSDDYFIKRKLYPNVDFYSGIIYQAMGFTPDQFTVLFGIPRTVGWLAQWQEMQ
QDPDKKIARPRQVYTGEDERDFVPIADRGARAMTAV

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=386, Percent_Identity=30.3108808290155, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1786939, Length=407, Percent_Identity=48.1572481572482, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1786527, Length=378, Percent_Identity=32.010582010582, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17555174, Length=372, Percent_Identity=31.1827956989247, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319850, Length=362, Percent_Identity=28.4530386740331, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6324328, Length=361, Percent_Identity=28.5318559556787, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325257, Length=395, Percent_Identity=26.0759493670886, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24640126, Length=429, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24640124, Length=429, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21356863, Length=393, Percent_Identity=26.972010178117, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 49554; Mature: 49554

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNETLTITDNRTQQTYTVPIYRGTVRAMDLRQIKKDADDFGLMTYDPAFMNTASCQSAI
CCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHHCCHHHCCEEEECHHHHCHHHHHHHH
TYLDGEKGILRYRGYPIEQLAEQCSFLEVAYLLLNGELPTAEQLEYWQCKVMRHTMTHEN
HHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VKKFMDGFHYDAHPMGILISTVAALSTFYPEAKNVGDAKCRHKQIVRLIGKMPTLAAYAY
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RHRLGLPYIAPDPELGYTKNYMNMLWKRTEPKYAANPVLARALEVLFILHADHEQNCSTS
HHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHEECCCCCCCCHH
TMRAVGSSNADPFVTTSAAIAALSGPLHGGANEEVLRMLDEIGKPENIPGFIEQVKEGKR
HHHHHCCCCCCCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KLMGFGHRVYKNYDPRARIIKQVAEEVFEVTGRNPKIDIALELEKIALSDDYFIKRKLYP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCEEEEECCC
NVDFYSGIIYQAMGFTPDQFTVLFGIPRTVGWLAQWQEMQQDPDKKIARPRQVYTGEDER
CCCHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCCCC
DFVPIADRGARAMTAV
CEECCCCCCCCHHCCC
>Mature Secondary Structure
MDNETLTITDNRTQQTYTVPIYRGTVRAMDLRQIKKDADDFGLMTYDPAFMNTASCQSAI
CCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHHCCHHHCCEEEECHHHHCHHHHHHHH
TYLDGEKGILRYRGYPIEQLAEQCSFLEVAYLLLNGELPTAEQLEYWQCKVMRHTMTHEN
HHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VKKFMDGFHYDAHPMGILISTVAALSTFYPEAKNVGDAKCRHKQIVRLIGKMPTLAAYAY
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RHRLGLPYIAPDPELGYTKNYMNMLWKRTEPKYAANPVLARALEVLFILHADHEQNCSTS
HHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHEECCCCCCCCHH
TMRAVGSSNADPFVTTSAAIAALSGPLHGGANEEVLRMLDEIGKPENIPGFIEQVKEGKR
HHHHHCCCCCCCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KLMGFGHRVYKNYDPRARIIKQVAEEVFEVTGRNPKIDIALELEKIALSDDYFIKRKLYP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCEEEEECCC
NVDFYSGIIYQAMGFTPDQFTVLFGIPRTVGWLAQWQEMQQDPDKKIARPRQVYTGEDER
CCCHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCCCC
DFVPIADRGARAMTAV
CEECCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]