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The map label for this gene is oprM [H]

Identifier: 116620698

GI number: 116620698

Start: 1931766

End: 1933253

Strand: Reverse

Name: oprM [H]

Synonym: Acid_1578

Alternate gene names: 116620698

Gene position: 1933253-1931766 (Counterclockwise)

Preceding gene: 116620699

Following gene: 116620697

Centisome position: 19.4

GC content: 64.99

Gene sequence:

>1488_bases
ATGATGCGCACAACTTCCCTATGGGCCGCGGCCTGCGCGATCCTGCTGCTCGCGGGGTGCACGGTCGGTCCGAATTACAA
GCGTCCTACGGCGCCGGCTCCGCCCGCCTTCAAAGAGCAGCCTCCGCCCAATTTCAAGGAGGCCGACGCAGCCGGCTGGA
AGCAGGCGCACCCGGGCGACGCGTTTCTCAAGGGGAAATGGTGGGAGGTCTACCAGGATCCGGCGCTCAACGCGCTCGAG
GAACAGGTGGCCCTGAATAATCAGAACGTAATGCAGGCGGAGGCGCAATTCCGGCAGGCGAAGGCCGCGGTCCGGGTGGC
TCGCGCCGCGCTCTTCCCCACCATCGGCACCGGAGCTTCCACCACGGTTTCGCGGGCACAGGGCGGGACCGGCGGCTTCG
GAAACAGCGGCAATTCCTCACCCGGCGCGCGCGTTGTGTACAGCCTGCCTGTCGACGCGAGCTGGGAGCCGGATCTCTGG
GGCAGCATCCGGCGCGGCGTGACCGGGGCCGCGGCCACGGCGCAATCCTTCGCCGCGGAAGTGGAAAACGCGCGGCTTCT
CTATCAATCCGAGCTGGCCTCCGATTACTTCCAGATGCACAGCATCGACGCCGAGATTGACCTGCTGAAGCGCACCGAAG
CGTCGTACGCCGAATATCTGACGCTGACGCAGAACCGTTTCAAGGGGGGCGTCGCGTCCGACCTGGATGTCGCACAGGCC
GAATCGCAGCTGTATGGAACTCAAGCATCGCTGATCGATCTAGGCGTGGAGCGCGCGGCCTTCGAACACGCGATCGCGAT
CCTGATCGGCAAAGCGCCCGCCGAGGTCACGCTGCCCTCTGCCTTGCTCGCCACACTGCCGCCCCCCGTGCCGGTCGGAG
TGCCCTCGCAATTGCTGGAGCGCAGGCCCGATATTGCCGCCTCCGAACGCCGGGTTGCCGCCGCCAACGAGCAAATCGGA
ATCGCGATGGCGGCGTTCTATCCGAATCTGACGCTCAGCGGCTCGGTCGGTCTTCAAAACTCCACGATCAGCCGCGTGAT
CTCCGCGCCTACCTATCTCTGGTCGCTCGGCGGCGGCCTGGCCTACACGTTATTCGATGCCGGACGCAGACGCGCAATCG
TGGTCAGCCAGCAGGCCGCCTATGATGCCACGGTGGCGGCGTACCGCGAAACTGTACTCACGGCCCTGCAGCAGGTGGAA
GACAATTTGGCCGCCCTGCGCATTCTGGAGCAGGAAGCCGCCAAGATCGGGGACACGGTTTCCTCCGCACAGCGAGCGCT
GAACATTTCCACCGCGCAGTATCGCGCCGGCACTACAAGTTATCTCACGGTGCTGACCTCGCAGGCCCAGTTGCTGAGCG
CGGAGCGCACCGCCGTCACCTTGCAATCGCGGCGCCTGACCTCCAGCGTCCAATTGATCGAAGCCATCGGCGGCGGCTGG
AATGCATCCCAACTGCCTTCGACACAGTCCGTCACCTCGACTAAATGA

Upstream 100 bases:

>100_bases
TCACCACTCCGGTGGTTTATCTTTATTTCGACCGCTTGCGGCTGTGGTGGGAACGCGTCTCCGCGCGCTTGCATGGGCGC
GAGCCGCTGGAGTCCACCAC

Downstream 100 bases:

>100_bases
TACGAGCCGCGAATGGTGATAAAGTTCCGGGAGAGAGGCCGACCATGAACCGTCGAGGATTTCTATTGAGCAGTGCGCTT
GCCGTTCCCTGCATCGCCCG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MMRTTSLWAAACAILLLAGCTVGPNYKRPTAPAPPAFKEQPPPNFKEADAAGWKQAHPGDAFLKGKWWEVYQDPALNALE
EQVALNNQNVMQAEAQFRQAKAAVRVARAALFPTIGTGASTTVSRAQGGTGGFGNSGNSSPGARVVYSLPVDASWEPDLW
GSIRRGVTGAAATAQSFAAEVENARLLYQSELASDYFQMHSIDAEIDLLKRTEASYAEYLTLTQNRFKGGVASDLDVAQA
ESQLYGTQASLIDLGVERAAFEHAIAILIGKAPAEVTLPSALLATLPPPVPVGVPSQLLERRPDIAASERRVAAANEQIG
IAMAAFYPNLTLSGSVGLQNSTISRVISAPTYLWSLGGGLAYTLFDAGRRRAIVVSQQAAYDATVAAYRETVLTALQQVE
DNLAALRILEQEAAKIGDTVSSAQRALNISTAQYRAGTTSYLTVLTSQAQLLSAERTAVTLQSRRLTSSVQLIEAIGGGW
NASQLPSTQSVTSTK

Sequences:

>Translated_495_residues
MMRTTSLWAAACAILLLAGCTVGPNYKRPTAPAPPAFKEQPPPNFKEADAAGWKQAHPGDAFLKGKWWEVYQDPALNALE
EQVALNNQNVMQAEAQFRQAKAAVRVARAALFPTIGTGASTTVSRAQGGTGGFGNSGNSSPGARVVYSLPVDASWEPDLW
GSIRRGVTGAAATAQSFAAEVENARLLYQSELASDYFQMHSIDAEIDLLKRTEASYAEYLTLTQNRFKGGVASDLDVAQA
ESQLYGTQASLIDLGVERAAFEHAIAILIGKAPAEVTLPSALLATLPPPVPVGVPSQLLERRPDIAASERRVAAANEQIG
IAMAAFYPNLTLSGSVGLQNSTISRVISAPTYLWSLGGGLAYTLFDAGRRRAIVVSQQAAYDATVAAYRETVLTALQQVE
DNLAALRILEQEAAKIGDTVSSAQRALNISTAQYRAGTTSYLTVLTSQAQLLSAERTAVTLQSRRLTSSVQLIEAIGGGW
NASQLPSTQSVTSTK
>Mature_495_residues
MMRTTSLWAAACAILLLAGCTVGPNYKRPTAPAPPAFKEQPPPNFKEADAAGWKQAHPGDAFLKGKWWEVYQDPALNALE
EQVALNNQNVMQAEAQFRQAKAAVRVARAALFPTIGTGASTTVSRAQGGTGGFGNSGNSSPGARVVYSLPVDASWEPDLW
GSIRRGVTGAAATAQSFAAEVENARLLYQSELASDYFQMHSIDAEIDLLKRTEASYAEYLTLTQNRFKGGVASDLDVAQA
ESQLYGTQASLIDLGVERAAFEHAIAILIGKAPAEVTLPSALLATLPPPVPVGVPSQLLERRPDIAASERRVAAANEQIG
IAMAAFYPNLTLSGSVGLQNSTISRVISAPTYLWSLGGGLAYTLFDAGRRRAIVVSQQAAYDATVAAYRETVLTALQQVE
DNLAALRILEQEAAKIGDTVSSAQRALNISTAQYRAGTTSYLTVLTSQAQLLSAERTAVTLQSRRLTSSVQLIEAIGGGW
NASQLPSTQSVTSTK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=474, Percent_Identity=25.7383966244726, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI2367348, Length=498, Percent_Identity=25.1004016064257, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52501; Mature: 52501

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRTTSLWAAACAILLLAGCTVGPNYKRPTAPAPPAFKEQPPPNFKEADAAGWKQAHPGD
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AFLKGKWWEVYQDPALNALEEQVALNNQNVMQAEAQFRQAKAAVRVARAALFPTIGTGAS
CEECCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TTVSRAQGGTGGFGNSGNSSPGARVVYSLPVDASWEPDLWGSIRRGVTGAAATAQSFAAE
CHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHH
VENARLLYQSELASDYFQMHSIDAEIDLLKRTEASYAEYLTLTQNRFKGGVASDLDVAQA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
ESQLYGTQASLIDLGVERAAFEHAIAILIGKAPAEVTLPSALLATLPPPVPVGVPSQLLE
HHHHHCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCHHHHH
RRPDIAASERRVAAANEQIGIAMAAFYPNLTLSGSVGLQNSTISRVISAPTYLWSLGGGL
CCCCCCCCCCHHHHCCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHCCHHHHHCCCCE
AYTLFDAGRRRAIVVSQQAAYDATVAAYRETVLTALQQVEDNLAALRILEQEAAKIGDTV
EEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSAQRALNISTAQYRAGTTSYLTVLTSQAQLLSAERTAVTLQSRRLTSSVQLIEAIGGGW
HHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCC
NASQLPSTQSVTSTK
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MMRTTSLWAAACAILLLAGCTVGPNYKRPTAPAPPAFKEQPPPNFKEADAAGWKQAHPGD
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AFLKGKWWEVYQDPALNALEEQVALNNQNVMQAEAQFRQAKAAVRVARAALFPTIGTGAS
CEECCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TTVSRAQGGTGGFGNSGNSSPGARVVYSLPVDASWEPDLWGSIRRGVTGAAATAQSFAAE
CHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHH
VENARLLYQSELASDYFQMHSIDAEIDLLKRTEASYAEYLTLTQNRFKGGVASDLDVAQA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
ESQLYGTQASLIDLGVERAAFEHAIAILIGKAPAEVTLPSALLATLPPPVPVGVPSQLLE
HHHHHCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCHHHHH
RRPDIAASERRVAAANEQIGIAMAAFYPNLTLSGSVGLQNSTISRVISAPTYLWSLGGGL
CCCCCCCCCCHHHHCCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHCCHHHHHCCCCE
AYTLFDAGRRRAIVVSQQAAYDATVAAYRETVLTALQQVEDNLAALRILEQEAAKIGDTV
EEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSAQRALNISTAQYRAGTTSYLTVLTSQAQLLSAERTAVTLQSRRLTSSVQLIEAIGGGW
HHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCC
NASQLPSTQSVTSTK
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]